BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0121.p (565 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MJ0277 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 285 2e-76 AF1720 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 279 1e-74 PAB0888 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a... 273 8e-73 MA3792 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 266 9e-71 MK0531 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 265 2e-70 >MJ0277 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Mja Methanococcus jannaschii Length = 591 Score = 285 bits (728), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 193/565 (34%), Positives = 291/565 (51%), Gaps = 51/565 (9%) Query: 1 MTGADVLVKLLEEYGVEVIFGVPGDTNVAFYKALNRSGKVRHVMARDERSALYMADAYAR 60 M GA+ ++K LE GV++IFG PG + FY AL S V H++ R E++A + AD +AR Sbjct: 1 MKGAEAIIKALEAEGVKIIFGYPGGAMLPFYDALYDSDLV-HILTRHEQAAAHAADGFAR 59 Query: 61 YSGKPGVAECPSGAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEGRGMITELDNAKLFE 120 SG+ GV SG GA + G+A A + S P+I LT +P K G E+D LF Sbjct: 60 ASGEAGVCVSTSGPGATNLVTGIATAYADSSPVIALTGQVPTKLIGNDAFQEIDALGLFM 119 Query: 121 PVCKLSFQVKTTAKIPEAVRRAFRTATSGRPGAVHLTFPEDILAHASASTPDEIYAEPEC 180 P+ K +FQ+K +IPE R AF AT+GRPG VH+ P+D+ E Y P Sbjct: 120 PITKHNFQIKKPEEIPETFRAAFEIATTGRPGPVHIDLPKDV---QDGEIDIEKYPIPAK 176 Query: 181 RRFPAFP---------MRPDADLVRQLAPRRPVLVAGGGVMHSDGGAALLALAEKIDAAV 231 P + ++ A L+ + RPV++AGGGV+ S LL LAE + V Sbjct: 177 VDLPGYKPKTVGHPLQIKKAAKLIAE--SERPVILAGGGVIISGASEELLRLAEFVKIPV 234 Query: 232 VTTITGQGAVPDEHPLALGVIGDNGFHPHAARAVEESDLLVYLGCKMGSVVT--IGWTFP 289 TT+ G+G P++HPLALG++G +G A AV E D+L+ +GC+ VT I + P Sbjct: 235 CTTLMGKGCFPEDHPLALGMVGMHGTK-AANYAVTECDVLIAIGCRFSDRVTGDIRYFAP 293 Query: 290 SHQQERQRVQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLLPLVTQHEPRAWNAELNGMRR 349 E + + +DIDPA +G R D+ I GD ++VL+DLL + E + L Sbjct: 294 ----EAKIIHIDIDPAEIGKNVRADIPIVGDAKNVLRDLLAALIALEIKDKETWL----E 345 Query: 350 RFWEQAQAD---LHSDALPLKPQR-------VVHELNRRLPPRAVMVSDAGTPTPHMTRY 399 R +E + + D P+KPQR V++E++ +L ++ +D G M + Sbjct: 346 RIYELKKLSIPMMDFDDKPIKPQRFVKDLMEVLNEIDSKL-KNTIITTDVGQNQMWMAHF 404 Query: 400 LRLPGDGSRLVIPRAF------GGLGYAIPAVVGAWLARPDVRPIGLFGDGSFSMSCGEL 453 + +PR+F G +G+ PA +GA +A+P I + GDG F M+ EL Sbjct: 405 FKTK-------MPRSFLASGGLGTMGFGFPAAIGAKVAKPYANVISITGDGGFLMNSQEL 457 Query: 454 ETLTRLQVPAVLLHFNNGCFGWIKALQKLHNDAQYLSVDFTRL-DVARIAQGFGLKGLRA 512 T++ +P V+ F+N G + Q L+ + V D ++A+ +G+K R Sbjct: 458 ATISEYDIPVVICIFDNRTLGMVYQWQNLYYGQRQSEVHLGESPDFVKLAESYGVKADRI 517 Query: 513 ANAEELVAALDEAFASEGPVLIDIV 537 + +E+ L EA S P L+DIV Sbjct: 518 ISPDEIKEKLKEAILSNEPYLLDIV 542 >AF1720 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Afu Archaeoglobus fulgidus Length = 552 Score = 279 bits (713), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 181/546 (33%), Positives = 277/546 (50%), Gaps = 29/546 (5%) Query: 1 MTGADVLVKLLEEYGVEVIFGVPGDTNVAFYKALNRSGKVRHVMARDERSALYMADAYAR 60 M AD +VK LE G+EV+FG+PG + Y AL SG +RH+ R E+ A + AD YAR Sbjct: 1 MRAADAIVKALEMEGIEVVFGIPGGAIIEVYDALYDSG-IRHITTRHEQGATHAADGYAR 59 Query: 61 YSGKPGVAECPSGAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEGRGMITELDNAKLFE 120 SGK GVA SG GA ++ G+A A S PL++ T +P G E D + Sbjct: 60 ASGKVGVAFATSGPGATNTVTGIATAYMDSSPLVVFTGQVPTSMIGNDAFQEADITGITM 119 Query: 121 PVCKLSFQVKTTAKIPEAVRRAFRTATSGRPGAVHLTFPEDILAHASASTPDEIYAEPEC 180 P+ K ++ V T + ++ AF A++GRPG V + P+DI +T D + P+ Sbjct: 120 PITKHNYLVTDTKDLLGTIKEAFHIASTGRPGPVLVDLPKDI------TTADIDFNYPKK 173 Query: 181 RRFPAFPMRPDADLVRQLAP--------RRPVLVAGGGVMHSDGGAALLALAEKIDAAVV 232 P + + + RQ+A RPV++AGGGV+ S+ L+ LAE I A VV Sbjct: 174 VSLPGYKPKLEGH-PRQIAKAAELIMKAERPVILAGGGVIISNASKELVELAETIPAFVV 232 Query: 233 TTITGQGAVPDEHPLALGVIGDNGFHPHAARAVEESDLLVYLGCKMGSVVTIGWTFPSHQ 292 TT+ G+G++P+ HPL+LG G +G +A A+ ESDL++ +GC+ T Sbjct: 233 TTLMGKGSIPETHPLSLGFAGMHG-TKYANYALSESDLIIAVGCRFSDRTT--GNVAMFA 289 Query: 293 QERQRVQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLLPLVTQHEPRAWNAELNGMRRRFW 352 E + + +DIDPA +G R D+ I GD R VL L V + + W ++N ++R+ Sbjct: 290 PEAKIIHIDIDPAEIGKNVRVDVPIVGDARKVLAKLKKAVEYKQRKEWEDKVNDWKKRY- 348 Query: 353 EQAQADLHSDALPLKPQRVVHELNRRLPPRAVMVSDAGTPTPHMTRYLRLPGDGSRLVIP 412 L KPQ V+ E + P A++ ++ G ++ + + + Sbjct: 349 -----PLKYKKEGFKPQYVI-ERACEIMPDAIITTEVGQNQMWAAQFFKTKYP-RQFITS 401 Query: 413 RAFGGLGYAIPAVVGAWLARPDVRPIGLFGDGSFSMSCGELETLTRLQVPAVLLHFNNGC 472 G +G+ PA +GA +A P+ I + GDGSF M+ EL T + ++P +L NNG Sbjct: 402 GGLGTMGFGFPAAMGAQVAFPEKTVIDIAGDGSFFMNIQELATCVKYEIPVKVLVLNNGY 461 Query: 473 FGWIKALQKLHNDAQY--LSVDFTRLDVARIAQGFGLKGLRAANAEELVAALDEAFASEG 530 G ++ Q+L +Y + + IA+GFG G+ E+ AL EA + Sbjct: 462 LGMVRQWQELFYRERYSATCIGCEKTGFEAIARGFGAIGMTVEKPSEVDDALKEAKEVDA 521 Query: 531 PVLIDI 536 PV+ID Sbjct: 522 PVVIDF 527 >PAB0888 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Pab Pyrococcus abyssi Length = 562 Score = 273 bits (698), Expect = 8e-73, Method: Compositional matrix adjust. Identities = 181/555 (32%), Positives = 294/555 (52%), Gaps = 37/555 (6%) Query: 1 MTGADVLVKLLEEYGVEVIFGVPGDTNVAFYKALNRSGKVRHVMARDERSALYMADAYAR 60 M+GA LVK LE+ GV+ FG+ G + + + L S +RH+ R E+ A + AD YAR Sbjct: 1 MSGARALVKALEKEGVKHTFGIIGGSIMPVFDELLESS-IRHITTRHEQGAAHAADGYAR 59 Query: 61 YSGKPGVAECPSGAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEGRGMITELDNAKLFE 120 SGKPGVA SG GA + G+A A S P++ +T +P G+ E D + + Sbjct: 60 VSGKPGVAITTSGPGATNLVTGIANAYMDSSPIVAITGQVPTHMIGKMAFQETDIISITK 119 Query: 121 PVCKLSFQVKTTAKIPEAVRRAFRTATSGRPGAVHLTFPEDILAHASASTPDEIYAEPEC 180 P+ K ++Q++ ++P+AV+ AF AT GRPG L FP+D + + DEI + + Sbjct: 120 PITKWNYQIRNPREVPKAVKMAFTIATLGRPGPTLLDFPKD-----AQTGEDEINFDVDV 174 Query: 181 RRF--PAFPMRPDA---------DLVRQLAPRRPVLVAGGGVMHSDGGAALLALAEKIDA 229 R P P + DA D++ L+ RPV + GGGV+ S +LA+AE + Sbjct: 175 SRLVTPWRPPKLDACPEEVKKAVDMI--LSAERPVFIVGGGVVWSGATEEVLAIAEYLMI 232 Query: 230 AVVTTITGQGAVPDEHPLALGVIGDNGFHPHAARAVEESDLLVYLGCKMGSVVTIGWTFP 289 +++T G+GAVP+ HPL +G +G +G A + + ++DL++ +G + S T+ F Sbjct: 233 PIMSTFMGKGAVPENHPLYIGNLGMHG-KIAANKLLPQTDLIIAVGMRW-SDRTVS-EFE 289 Query: 290 SHQQERQRVQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLLPLVTQHEPRAWNAELNGMRR 349 + E + + +DIDP +G + DL I GD + VL+ + +T+ + + R Sbjct: 290 NFAPEAKIIHIDIDPKEVGKNVKVDLGIVGDAKRVLRSIYSEITRGVKKREDWPWLKKVR 349 Query: 350 RFWEQAQADLHS-DALPLKPQRVVHELNRRLPPRAVMVSDAGTPTPHMTRYLRLPGDGSR 408 F E+ + +L D L+P ++ EL + LP A++ ++ G M L P Sbjct: 350 EFQEKYKEELIPLDGNYLRPPEILKELRKILPEDAIVATEVG--QNQMWVALYFP----- 402 Query: 409 LVIPRAF------GGLGYAIPAVVGAWLARPDVRPIGLFGDGSFSMSCGELETLTRLQVP 462 ++ PR F G +G+ PA +GA +A+PD I + GDGSF MS EL T +P Sbjct: 403 ILKPRTFLTSGGLGTMGFGFPAAIGAKVAKPDKVVIDIAGDGSFMMSERELATAVNENLP 462 Query: 463 AVLLHFNNGCFGWIKALQKLHNDAQYLSVDFTRL-DVARIAQGFGLKGLRAANAEELVAA 521 +++ NN G + Q++ + +Y++ F + D ++A+ +G +G RA EEL+ A Sbjct: 463 VIVVILNNSSLGMVAQWQRMFFNRRYVATYFKKNPDFVKLAEAYGAQGFRAETMEELLKA 522 Query: 522 LDEAFASEGPVLIDI 536 + E SE P +ID+ Sbjct: 523 VKEGMNSEVPTVIDV 537 >MA3792 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Mac Methanosarcina acetivorans str.C2A Length = 564 Score = 266 bits (680), Expect = 9e-71, Method: Compositional matrix adjust. Identities = 178/563 (31%), Positives = 295/563 (52%), Gaps = 37/563 (6%) Query: 1 MTGADVLVKLLEEYGVEVIFGVPGDTNVAFYKALNRSGKVRHVMARDERSALYMADAYAR 60 + GA L+K LE+ GV+ +FG PG + FY L S +RH++ R E++A + AD YAR Sbjct: 9 VNGAKALIKCLEKEGVDTLFGYPGGQIIPFYNELYDS-DLRHILVRHEQAAAHAADGYAR 67 Query: 61 YSGKPGVAECPSGAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEGRGMITELDNAKLFE 120 +GK GV SG GA + G+A A S+P++ LT +P G E D + Sbjct: 68 ATGKTGVCVSTSGPGATNLVTGIATAYMDSVPIVALTGQVPRPLIGNDAFQEADITGITL 127 Query: 121 PVCKLSFQVKTTAKIPEAVRRAFRTATSGRPGAVHLTFPEDIL-AHASASTPDEIYAEPE 179 P+ K ++ V+ +IP V+ AF A++GRPG V + P+D+ PD++ E Sbjct: 128 PITKHNYLVQDAEEIPRIVKEAFHIASTGRPGPVLIDLPKDVQNIEIDLQYPDKV----E 183 Query: 180 CRRF-PAFPMRPDADLVRQLAPR-----RPVLVAGGGVMHSDGGAALLALAEKIDAAVVT 233 R + P + + + +++ A RP++ AGGGV+ S+ A L+ LAE + A V T Sbjct: 184 LRGYKPTY--KGNTQQIKRAAEEIANSCRPIIYAGGGVISSNASAELVELAETLMAPVTT 241 Query: 234 TITGQGAVPDEHPLALGVIGDNGFHPHAARAVEESDLLVYLGCKMGSVVTIGWTFPSHQQ 293 T+ G A+P EHPL +G++G +G +A AV+ESDL++ +G + VT S Sbjct: 242 TLMGISAIPTEHPLYVGMLGMHGCK-YANYAVQESDLIIAVGARFDDRVT--GKLESFAP 298 Query: 294 ERQRVQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLLPLVTQHEPRAWNAELNGMRRRFWE 353 + + +D+DPA + + + I GD + +L+ L+ + + W ++N ++ + Sbjct: 299 NARVIHIDVDPAEISKNVKVHVPIVGDAKQILKSLIKYIQRCNSAEWIEKINQWKKEY-P 357 Query: 354 QAQADLHSDALPLKPQRVVHELNRRLPPRAVMVSDAGTPTPHMTRYLRLPGDGSRLVIPR 413 A + SD + PQ VV +++ + A++V++ G +Y + PR Sbjct: 358 LAYRYVSSDT--IMPQFVVEQIS-EVCKDAIIVTEVGQHQMWAAQYFKYRK-------PR 407 Query: 414 AF------GGLGYAIPAVVGAWLARPDVRPIGLFGDGSFSMSCGELETLTRLQVPAVLLH 467 F G +GY +PA +GA + +PD I + GDGSF M+ EL TL + +P V + Sbjct: 408 TFLTSGGLGTMGYGLPAAMGAKVGKPDKTVINIAGDGSFQMNSQELATLVQNDIPVVSVI 467 Query: 468 FNNGCFGWIKALQKLHNDAQYLSVDFTR--LDVARIAQGFGLKGLRAANAEELVAALDEA 525 NNG G ++ Q+L D +Y S F + +D ++A+ +G GLRA E+ A++EA Sbjct: 468 LNNGYLGMVRQWQELFYDRRY-SHTFIKGSVDFVKLAEAYGALGLRAEKPSEVRPAIEEA 526 Query: 526 FASEGPVLIDIVSESIVDECPPV 548 S P +++++ E + P V Sbjct: 527 VGSGRPAVVEVIVECEANVYPMV 549 >MK0531 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Mka Methanopyrus kandleri AV19 Length = 565 Score = 265 bits (677), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 177/558 (31%), Positives = 283/558 (50%), Gaps = 21/558 (3%) Query: 2 TGADVLVKLLEEYGVEVIFGVPGDTNVAFYKALNRSGKVRHVMARDERSALYMADAYARY 61 TGA VLV+ L+E GVE IFG PG + Y + + H++ R E+ A + AD YAR Sbjct: 6 TGARVLVECLKEEGVEHIFGYPGGAVLPIYDEIYDEVSIEHILVRHEQGAAHAADGYARV 65 Query: 62 SGKPGVAECPSGAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEGRGMITELDNAKLFEP 121 GKPGV SG GA + G+A A S P+I +T +P G+ E+D +F P Sbjct: 66 KGKPGVCMATSGPGATNLVTGIATAYMDSSPVIAITGQVPTTMIGKDAFQEVDAVGVFMP 125 Query: 122 VCKLSFQVKTTAKIPEAVRRAFRTATSGRPGAVHLTFPEDIL-AHASASTPDEIYAE--P 178 + K ++Q+ +IPE V+ AF+ A +GRPG VH+ P+D+ A P + E Sbjct: 126 ITKHNYQIGKPEEIPEVVKEAFKIAITGRPGPVHIDVPKDVQEAEVDVEIPKTVEVEGLN 185 Query: 179 ECRRFPAFPMRPDADLVRQLAPRRPVLVAGGGVMHSDGGAALLALAEKIDAAVVTTITGQ 238 +R ++ A+L+ + RPV++AGGG + S+ L+ LAE + A V TT+ G+ Sbjct: 186 VVKRGHPVQIKRAAELLAE--AERPVILAGGGCVISNATRELIELAELLGAPVATTLMGK 243 Query: 239 GAVPDEHPLALGVIGDNGFHPHAARAVEESDLLVYLGCKMGSVVTIGWTFPS-HQQERQR 297 GA P++HPLALG+ G +G A A+ E D+L+ +GC+ T PS E + Sbjct: 244 GAFPEDHPLALGMAGMHGTKA-ANYALTECDVLLAVGCRFSDRTT---GDPSGFAPEAKI 299 Query: 298 VQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLLPLVTQ----HEPRAWNAELNGMRRRFWE 353 + +DIDPA +G D+ I GD + VL+DL+ + + E + W + ++ E Sbjct: 300 IHIDIDPAEIGKNIPVDVPIVGDAKLVLRDLIKELKRRKYLRERKRWGERIEELKAEV-E 358 Query: 354 QAQADLHSDALPLKPQRVVHELNRRLPPR-AVMVSDAGTPTPHMTRYLRLPGDGSRLVIP 412 SD + P+ +V L+ L R ++ +D G M RY + + R + Sbjct: 359 MPPESTESDQR-ISPRELVRVLHEALKDRDYILTTDVGQNQMWMARYFPVE-EPRRFISS 416 Query: 413 RAFGGLGYAIPAVVGAWLARPDVRPIGLFGDGSFSMSCGELETLTRLQVPAVLLHFNNGC 472 G +G+ +PA +GA +A P+ + + GDG F M+ EL T + + +N Sbjct: 417 GGLGTMGFGLPAALGAKVAAPEKTVVAVVGDGGFLMTAQELATAVDNDIEVKVFVMDNRL 476 Query: 473 FGWIKALQKLHNDAQYLSVDF-TRLDVARIAQGFGLKGLRAANAEELVAALDEAFASEGP 531 G + Q+L D + + D+ ++ + +G G+ EL +A++EAF + G Sbjct: 477 LGMVAQWQRLFYDERLSESKLDEKTDIVKLTESYGAAGITVEEPSELESAVEEAFETPGT 536 Query: 532 VLIDIV--SESIVDECPP 547 V++D+ E I+ PP Sbjct: 537 VVVDVFVDPEEIIPMVPP 554