BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0032.p (370 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1339 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 553 e-157 PA2356 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 532 e-151 ECs1018 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 522 e-148 ycbN [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrah... 521 e-147 ZycbN [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetra... 520 e-147 >RSc1339 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Rso Ralstonia solanacearum Length = 387 Score = 553 bits (1425), Expect = e-157, Method: Compositional matrix adjust. Identities = 270/363 (74%), Positives = 314/363 (86%), Gaps = 4/363 (1%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARPVTHDYMKQIAVAADTLGYDGVLLPTGRSCEDAWVAA 60 M VFWF+PTHGDSRYLGT+EGAR V Y++Q+AVAADTLGY+GVL+PTGRSCED W+ A Sbjct: 1 MQVFWFLPTHGDSRYLGTAEGARQVDQAYLQQVAVAADTLGYEGVLIPTGRSCEDPWIVA 60 Query: 61 ASLIDATQRLKFLVAVRPGLTAPTLSARMAATFDRLSNGRLLINVVTGGDPVEQESDGIF 120 ASLI AT+RL+FLVAVRPGL APTL+ARMAATFDRLS GRLL+N+VTGGDP E DG+F Sbjct: 61 ASLIPATRRLRFLVAVRPGLVAPTLAARMAATFDRLSQGRLLVNLVTGGDPGELAGDGLF 120 Query: 121 GSHAERYEISDEFLKIWREVLAKTHTGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGG 180 HA+RYE S EF++IWRE LA +H G ++ G+HLSVKGA+ L+PPVQ P+PP+YFGG Sbjct: 121 LDHAQRYEASAEFIRIWRETLAASHEGAALDYTGRHLSVKGARVLFPPVQRPHPPVYFGG 180 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRDTNEA 240 SS+ A LAAEQVD YLTWGEPPAAVAEKIA VR RAA+ GRT+RFGIRLHVIVR+T +A Sbjct: 181 SSEAAHALAAEQVDTYLTWGEPPAAVAEKIADVRRRAARHGRTVRFGIRLHVIVRETEDA 240 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALH--GGRRDK--LEVSPNLWAGV 296 AW+AAD LIS+LDD+T+A+AQA F +MDS GQ+RMAALH G RR + LE+SPNLWAGV Sbjct: 241 AWQAADTLISKLDDDTVARAQAAFRKMDSAGQQRMAALHANGIRRSRAELEISPNLWAGV 300 Query: 297 GLVRGGAGTALVGDGPTVAARIKEYADLGIDTFILSGYPHLEESYRFAELVFPLLPRAQR 356 GLVRGGAGTALVGD TVAAR++EYADLGIDTF+LSGYPHLEE+YRFAELVFPLLPRA R Sbjct: 301 GLVRGGAGTALVGDPHTVAARMREYADLGIDTFVLSGYPHLEEAYRFAELVFPLLPRAVR 360 Query: 357 EGL 359 + L Sbjct: 361 DTL 363 >PA2356 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 381 Score = 532 bits (1370), Expect = e-151, Method: Compositional matrix adjust. Identities = 257/352 (73%), Positives = 296/352 (84%), Gaps = 4/352 (1%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARPVTHDYMKQIAVAADTLGYDGVLLPTGRSCEDAWVAA 60 MNVFWF+PTHGD +LGTS+GARPV+ Y+KQ+A AAD+LGY GVL+PTGRSCED+WV A Sbjct: 1 MNVFWFLPTHGDGHFLGTSQGARPVSLPYLKQVAQAADSLGYHGVLIPTGRSCEDSWVVA 60 Query: 61 ASLIDATQRLKFLVAVRPGLTAPTLSARMAATFDRLSNGRLLINVVTGGDPVEQESDGIF 120 ++L T+RL+FLVA+RPG+ +PT+SARMAAT DRLS GRLLINVVTGGDP E DGI Sbjct: 61 SALAPLTERLRFLVAIRPGIVSPTVSARMAATLDRLSGGRLLINVVTGGDPDENRGDGIH 120 Query: 121 GSHAERYEISDEFLKIWREVLAKTHTGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGG 180 HAERYE++DEFL++WR VL GE +F GKH+ V+ AKALYPP+Q PYPPLYFGG Sbjct: 121 LGHAERYEVTDEFLRVWRRVL----QGEAVDFHGKHIHVENAKALYPPLQRPYPPLYFGG 176 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRDTNEA 240 SS+ A +LA EQVDVYLTWGEP AVA KIA VR+RAA+ GRT++FGIRLHVIVR+T E Sbjct: 177 SSEAAHELAGEQVDVYLTWGEPLPAVAAKIADVRQRAARHGRTVKFGIRLHVIVRETAEE 236 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 AWRAAD LI + DETIA AQ +FAR DSEGQRRMAALHGGRRD+LE+ PNLWAGVGLVR Sbjct: 237 AWRAADRLIEHISDETIAAAQQSFARFDSEGQRRMAALHGGRRDRLEIQPNLWAGVGLVR 296 Query: 301 GGAGTALVGDGPTVAARIKEYADLGIDTFILSGYPHLEESYRFAELVFPLLP 352 GGAGTALVGD VA RI EYA+LGID+FI SGYPHLEE+YRFAELVFPLLP Sbjct: 297 GGAGTALVGDPRQVAERIGEYAELGIDSFIFSGYPHLEEAYRFAELVFPLLP 348 >ECs1018 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Ecs Escherichia coli O157:H7 Length = 381 Score = 522 bits (1344), Expect = e-148, Method: Compositional matrix adjust. Identities = 249/351 (70%), Positives = 290/351 (82%), Gaps = 4/351 (1%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARPVTHDYMKQIAVAADTLGYDGVLLPTGRSCEDAWVAA 60 +N+FWF+PTHGD YLGT EG+RPV H Y++QIA AAD LGY GVL+PTGRSCEDAW+ A Sbjct: 3 LNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGYTGVLIPTGRSCEDAWLVA 62 Query: 61 ASLIDATQRLKFLVAVRPGLTAPTLSARMAATFDRLSNGRLLINVVTGGDPVEQESDGIF 120 AS+I TQRLKFLVA+RP +T+PT++AR AAT DRLSNGR L N+VTG DP E DG+F Sbjct: 63 ASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGRALFNLVTGSDPQELAGDGVF 122 Query: 121 GSHAERYEISDEFLKIWREVLAKTHTGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGG 180 H+ERYE S EF ++WR +L GE +F GKH+ V+GAK L+PP+Q PYPPLYFGG Sbjct: 123 LDHSERYEASAEFTQVWRRLL----LGETVDFNGKHIHVRGAKLLFPPIQQPYPPLYFGG 178 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRDTNEA 240 SSD A +LAAEQVD+YLTWGEPP V EKI +VR +AA GR +RFGIRLHVIVR+TN+ Sbjct: 179 SSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAHGRKIRFGIRLHVIVRETNDE 238 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 AW+AA+ LIS LDDETIAKAQA FAR DS GQ+RMAALH G+RD LE+SPNLWAGVGLVR Sbjct: 239 AWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWAGVGLVR 298 Query: 301 GGAGTALVGDGPTVAARIKEYADLGIDTFILSGYPHLEESYRFAELVFPLL 351 GGAGTALVGDGPTVAARI EYA LGID+F+LSGYPHLEE+YR EL+FP L Sbjct: 299 GGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPHLEEAYRVGELLFPHL 349 >ycbN [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Eco Escherichia coli K12 Length = 381 Score = 521 bits (1342), Expect = e-147, Method: Compositional matrix adjust. Identities = 248/351 (70%), Positives = 290/351 (82%), Gaps = 4/351 (1%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARPVTHDYMKQIAVAADTLGYDGVLLPTGRSCEDAWVAA 60 +N+FWF+PTHGD YLGT EG+RPV H Y++QIA AAD LGY GVL+PTGRSCEDAW+ A Sbjct: 3 LNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGYTGVLIPTGRSCEDAWLVA 62 Query: 61 ASLIDATQRLKFLVAVRPGLTAPTLSARMAATFDRLSNGRLLINVVTGGDPVEQESDGIF 120 AS+I TQRLKFLVA+RP +T+PT++AR AAT DRLSNGR L N+VTG DP E DG+F Sbjct: 63 ASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGRALFNLVTGSDPQELAGDGVF 122 Query: 121 GSHAERYEISDEFLKIWREVLAKTHTGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGG 180 H+ERYE S EF ++WR +L + E +F GKH+ V+GAK L+P +Q PYPPLYFGG Sbjct: 123 LDHSERYEASAEFTQVWRRLLQR----ETVDFNGKHIHVRGAKLLFPAIQQPYPPLYFGG 178 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRDTNEA 240 SSD A +LAAEQVD+YLTWGEPP V EKI +VR +AA GR +RFGIRLHVIVR+TN+ Sbjct: 179 SSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAHGRKIRFGIRLHVIVRETNDE 238 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 AW+AA+ LIS LDDETIAKAQA FAR DS GQ+RMAALH G+RD LE+SPNLWAGVGLVR Sbjct: 239 AWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWAGVGLVR 298 Query: 301 GGAGTALVGDGPTVAARIKEYADLGIDTFILSGYPHLEESYRFAELVFPLL 351 GGAGTALVGDGPTVAARI EYA LGID+F+LSGYPHLEE+YR EL+FPLL Sbjct: 299 GGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPHLEEAYRVGELLFPLL 349 >ZycbN [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases EcZ Escherichia coli O157:H7 EDL933 Length = 381 Score = 520 bits (1339), Expect = e-147, Method: Compositional matrix adjust. Identities = 248/351 (70%), Positives = 289/351 (82%), Gaps = 4/351 (1%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARPVTHDYMKQIAVAADTLGYDGVLLPTGRSCEDAWVAA 60 +N+FWF+PTHGD YLGT EG+RPV H Y++QIA AAD LGY GVL+PTGRSCEDAW+ A Sbjct: 3 LNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGYTGVLIPTGRSCEDAWLVA 62 Query: 61 ASLIDATQRLKFLVAVRPGLTAPTLSARMAATFDRLSNGRLLINVVTGGDPVEQESDGIF 120 AS+I TQRLKFLVA+RP +T+PT++AR AAT DRLSNGR L N+VTG DP E DG+F Sbjct: 63 ASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGRALFNLVTGSDPQELAGDGVF 122 Query: 121 GSHAERYEISDEFLKIWREVLAKTHTGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGG 180 H+ERYE S EF ++WR +L GE +F GKH+ V+GAK L+PP+Q PYPPLYFGG Sbjct: 123 LDHSERYEASAEFTQVWRRLL----LGETVDFNGKHIHVRGAKLLFPPIQQPYPPLYFGG 178 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRDTNEA 240 SSD A +LAAEQVD+YLTWGEPP V EKI +VR +AA GR +RFGIRLHVIVR+TN+ Sbjct: 179 SSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAHGRKIRFGIRLHVIVRETNDE 238 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 AW+AA+ LIS LDDETIAKAQA FAR DS GQ+RMAALH G+RD LE+SPNLWAGVGL R Sbjct: 239 AWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWAGVGLXR 298 Query: 301 GGAGTALVGDGPTVAARIKEYADLGIDTFILSGYPHLEESYRFAELVFPLL 351 GGAGTALVGDGPTVAARI EYA LGID+F+LSGYPHLEE+YR EL+FP L Sbjct: 299 GGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPHLEEAYRVGELLFPHL 349