BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0004.p (408 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2244 [L] COG3569 Topoisomerase IB Pae Pseudomonas aeruginosa 341 2e-93 mll2873 Non COG 244 3e-64 DR0690 [L] COG3569 Topoisomerase IB Dra Deinococcus radiodurans 239 6e-63 AGl1631 [L] COG3569 Topoisomerase IB Atu Agrobacterium tumefacie... 238 2e-62 SMb21445 [L] COG3569 Topoisomerase IB Sme Sinorhizobium meliloti 235 2e-61 >PA2244 [L] COG3569 Topoisomerase IB Pae Pseudomonas aeruginosa Length = 333 Score = 341 bits (874), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 173/330 (52%), Positives = 224/330 (67%), Gaps = 15/330 (4%) Query: 39 LRYVNDGMPGIRRRRRGKGFAYHDAHGELIRDDDVVARLKSLAIPPAWEDVWISPHANGH 98 L YV+D PGIRRRR+GK F Y DA G+ I D + + RL LAIPPA+ +VWI P NGH Sbjct: 15 LCYVDDRQPGIRRRRQGKRFVYFDADGQRIGDAEEIRRLDKLAIPPAYREVWICPDPNGH 74 Query: 99 IQATGRDARGRKQYRYHARWRSVRDDAKYLRMISFARALPTIRRVVEQDLKRPGLHRQKV 158 +QATGRDARGRKQYRYH RWR VRD KY R++ F +ALP +RR ++ L+ PGL R KV Sbjct: 75 LQATGRDARGRKQYRYHPRWREVRDADKYERLLRFGQALPRLRRHIDAQLRLPGLGRDKV 134 Query: 159 LAAVVHLLQTTFMRVGNDEYAKQNQSFGLTTLRHRHVRVDGSDVLFRFRGKSGVQHDIKL 218 +A V LL T +RVGN YA+ N+S+GLTTLR RHV V+GS + FRF+GKSG++HD+ L Sbjct: 135 VALAVALLDATLIRVGNHRYARDNRSYGLTTLRTRHVDVNGSRIRFRFKGKSGIEHDVSL 194 Query: 219 HDPYVARIIRKMRELPGQELFQYVDDEGERHCIDSGDVNAYLREAAGEDFSAKDFRTWAG 278 P +AR++R+ ELPGQ+L QY+D++G+ H I S D+N YLR GEDFSAKD+RTWAG Sbjct: 195 EHPRLARVLRRCLELPGQDLLQYLDEDGQPHRIGSHDINEYLRRHTGEDFSAKDYRTWAG 254 Query: 279 TVLATLALQAFERVDSQAQAKQNVVRAIENVARRLGNTPSICRKCYVHPAVIESYLDGSF 338 + LAL+ R ++ + VA LGN+ +ICR+CY+HPA+IE+Y G Sbjct: 255 S---ALALERLRRAEADGLGA-----VVAEVAAELGNSVAICRQCYIHPAIIEAYQAGQL 306 Query: 339 AERLEERVQAELVEELQDLSPEEAAVLALL 368 Q + + LS EEA +LA L Sbjct: 307 G-------QLRRARKRRWLSAEEATLLAFL 329 >mll2873 Non COG Length = 288 Score = 244 bits (623), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 124/244 (50%), Positives = 164/244 (67%), Gaps = 1/244 (0%) Query: 10 QSSSSSPSAPPPADARHQDEAIDIARANQLRYVNDGMPGIRRRRRGKGFAYHDAHGELIR 69 Q S P DA ++ A A L YV+D PGIRR + GKGF+Y G+ + Sbjct: 3 QRSGLPPGRSTDGDAAPKNGAQSAAEHAALTYVSDAEPGIRRLKTGKGFSYKGPDGKAV- 61 Query: 70 DDDVVARLKSLAIPPAWEDVWISPHANGHIQATGRDARGRKQYRYHARWRSVRDDAKYLR 129 D AR++++ IPPAW DVWIS ANGHIQATGRD RGRKQYRYH +W RD AKY Sbjct: 62 SDAARARIEAIVIPPAWTDVWISLDANGHIQATGRDQRGRKQYRYHPQWAKERDGAKYSS 121 Query: 130 MISFARALPTIRRVVEQDLKRPGLHRQKVLAAVVHLLQTTFMRVGNDEYAKQNQSFGLTT 189 +++FA +L ++RR ++ DL+R GL ++V+AAVV LL T +RVGN YA+ NQSFGLTT Sbjct: 122 LVAFAESLASLRRTIDSDLRRHGLPFERVVAAVVWLLDNTMIRVGNAAYARDNQSFGLTT 181 Query: 190 LRHRHVRVDGSDVLFRFRGKSGVQHDIKLHDPYVARIIRKMRELPGQELFQYVDDEGERH 249 LR RHV + GS + F F+GKSG + ++L D +A I+R ++LPGQ+LFQY+D++G R Sbjct: 182 LRDRHVDIKGSSLRFAFKGKSGKEWKLRLVDRRIAGIVRGAQDLPGQKLFQYLDEDGSRR 241 Query: 250 CIDS 253 I S Sbjct: 242 PIRS 245 >DR0690 [L] COG3569 Topoisomerase IB Dra Deinococcus radiodurans Length = 346 Score = 239 bits (611), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 139/315 (44%), Positives = 192/315 (60%), Gaps = 7/315 (2%) Query: 58 FAYHDAHGELIRDDDVVARLKSLAIPPAWEDVWISPHANGHIQATGRDARGRKQYRYHAR 117 F Y G D D +AR+ LA+PPA++DV++SP A +QA GRDA GR QYRYH Sbjct: 23 FRYVHPDGTPYTDADGLARIARLAVPPAYQDVYVSPDAENELQAFGRDAAGRLQYRYHPD 82 Query: 118 WRSVRDDAKYLRMISFARALPTIRRVVEQDLKRPGLHRQKVLAAVVHLLQTTFMRVGNDE 177 + K+ R+ FA ALPT++ DL+ GL +KV+A + LL RVG+D Sbjct: 83 FVQAGALKKWQRLTRFAGALPTLKVATTADLRASGLPPRKVMALMTRLLHVARFRVGSDI 142 Query: 178 YAKQNQSFGLTTLRHRHVRVDGSDVLFRFRGKSGVQHDIKLHDPYVARIIRKMRELPGQE 237 YA+Q++++GL+TLR RHV VDG+ V FRF+GK GV D +A ++K+ +LPG Sbjct: 143 YARQHKTYGLSTLRQRHVVVDGNTVTFRFKGKHGVSQHKATSDRTLAANMQKLLDLPGPW 202 Query: 238 LFQYVD-DEGERHCIDSGDVNAYLREAAGEDFSAKDFRTWAGTVLAT--LALQAFERVDS 294 LFQ VD GER I S ++NAYLRE G F+AKDFRTW GT+LA LA Q E S Sbjct: 203 LFQTVDAGGGERRRIHSTELNAYLREVIGP-FTAKDFRTWGGTLLAAEYLAQQGTE--SS 259 Query: 295 QAQAKQNVVRAIENVARRLGNTPSICRKCYVHPAVIESYLDGSFAERLEERVQAELVEEL 354 + QAK+ +V ++ VA LGNTP++ R Y+ P + + YLDG + E R + + EL Sbjct: 260 ERQAKKVLVDCVKFVADDLGNTPAVTRGSYICPVIFDRYLDGKVLDDYEPRTERQEA-EL 318 Query: 355 QDLSPEEAAVLALLQ 369 + L+ E A+ +L+ Sbjct: 319 EGLTRSEGALKRMLE 333 >AGl1631 [L] COG3569 Topoisomerase IB Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 338 Score = 238 bits (607), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 130/293 (44%), Positives = 177/293 (60%), Gaps = 5/293 (1%) Query: 39 LRYVNDGMPGIRRRRRGKGFAYHDAHGELIRDDDVVARLKSLAIPPAWEDVWISPHANGH 98 L YVND PGI R +RG+GF Y G+L+ D + R+KSL +PPA+ DVWI GH Sbjct: 14 LAYVNDQEPGIAREKRGRGFCYRLPGGDLLSDSVELKRIKSLGVPPAYRDVWICIDPAGH 73 Query: 99 IQATGRDARGRKQYRYHARWRSVRDDAKYLRMISFARALPTIRRVVEQDLKRPGLHRQKV 158 +QATG DARGRKQYRYH W ++R + K+ ++ SF +ALP IRR D+ + ++ Sbjct: 74 LQATGFDARGRKQYRYHPDWHALRGETKFFQLKSFGKALPAIRRRAMADIGKQDHGQEMT 133 Query: 159 LAAVVHLLQTTFMRVGNDEYAKQNQSFGLTTLRHRHVRVDGSDVLFRFRGKSGVQHDIKL 218 LAA+ LL ++RVGN Y + N ++G TTL RHV G + RF K G + +L Sbjct: 134 LAALTLLLDAAYLRVGNRSYLETNGTYGATTLLKRHVSF-GETIELRFAAKGGQKVKRQL 192 Query: 219 HDPYVARIIRKMRELPGQELFQYVDDEGERHCIDSGDVNAYLREAAGEDFSAKDFRTWAG 278 P + +I+ ++ +LPG+ELF + D E H +DS D+NAYL G+ SAK FRTW G Sbjct: 193 RHPRLQKILEEIADLPGKELFVWQDSENRVHSVDSSDLNAYLSGIGGQGISAKTFRTWGG 252 Query: 279 TVLATLALQAFERVDSQAQAKQNVVRAIENVARRLGNTPSICRKCYVHPAVIE 331 T+ A A E V A K ++ + A L NTP+ICRK YVHPAV++ Sbjct: 253 TLAAF--CNAMEHV--AAGEKPSIKGMCQAAAAELSNTPAICRKSYVHPAVLD 301 >SMb21445 [L] COG3569 Topoisomerase IB Sme Sinorhizobium meliloti Length = 351 Score = 235 bits (599), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 119/293 (40%), Positives = 178/293 (60%), Gaps = 6/293 (2%) Query: 39 LRYVNDGMPGIRRRRRGKGFAYHDAHGELIRDDDVVARLKSLAIPPAWEDVWISPHANGH 98 L YV+D PGIRR+RRGKGF Y G ++ D + +R+ +L +PPA+++VWI GH Sbjct: 7 LVYVSDSEPGIRRQRRGKGFVYRMPDGSIVTDPSIKSRIAALGLPPAYDNVWICLEERGH 66 Query: 99 IQATGRDARGRKQYRYHARWRSVRDDAKYLRMISFARALPTIRRVVEQDLKRPGLHRQKV 158 +QATG DARGRKQYRYH+ W+++R K+ +++ F +ALP IRR + + ++ + Q V Sbjct: 67 LQATGYDARGRKQYRYHSEWQALRSADKFAQLVEFGKALPKIRRTIRRHMQGDVENMQTV 126 Query: 159 LAAVVHLLQTTFMRVGNDEYAKQNQSFGLTTLRHRHVRVDGSDVLFRFRGKSGVQHDIKL 218 LAA+V LL +R GN Y + N S+G TTL RH+R+ + +F GK G + L Sbjct: 127 LAALVALLDEAHLRTGNQAYVQANGSYGATTLLKRHLRLGDGFIELKFIGKGGKRVQRLL 186 Query: 219 HDPYVARIIRKMRELPGQELFQYVDDEGERHCIDSGDVNAYLREAAGEDFSAKDFRTWAG 278 P + +++ ++ +LPG++LF + D+ +DSG +N YL + AG SAK FRTW G Sbjct: 187 RRPKLQQLLEEIADLPGRQLFVWKDENDVLRPVDSGRLNRYLSDVAGTAVSAKTFRTWGG 246 Query: 279 TVLATLALQAFERVDSQAQAKQNVVRAI-ENVARRLGNTPSICRKCYVHPAVI 330 T+ A A + S Q + ++ + E A L NTP+I R Y+HP V+ Sbjct: 247 TLAAFTAART-----SIEQGEWPTIKQMSEAAASVLHNTPAISRSSYIHPDVL 294