BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0133.p (633 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value APE1307 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming... 410 e-114 PAE2464_1 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-formi... 328 2e-89 PM0925 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 260 6e-69 YPO0537 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming... 256 1e-67 HI0002 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 244 5e-64 >APE1307 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Ape Aeropyrum pernix Length = 651 Score = 410 bits (1054), Expect = e-114, Method: Compositional matrix adjust. Identities = 236/635 (37%), Positives = 351/635 (55%), Gaps = 27/635 (4%) Query: 5 EGNGSFEPAQEGGKW-----WRRDKTRK--XSASSFACGLAALGFKRGMSLAIIGNNCPR 57 E +P + +W WRR R + A L LG G + A I N P Sbjct: 28 EWRARTQPGRVAFRWKRYGIWRRYTWRDYYLRVAYAALALENLGLGPGDTGAFITFNRPS 87 Query: 58 LYWAMSAAQALGGMPVPLYQDAPAADMAYVLADANVDFVMAEDQEQVDKVFEIKENLPRI 117 Q LGG + +Y+D+ + ++ Y+L + FV+ E QEQ+D+V + ++ +I Sbjct: 88 WVIYEVGVQLLGGRAMGIYRDSLSDEVGYLLDSGDAKFVLVEGQEQLDRVLDSGVDVDKI 147 Query: 118 AHVIYDDERGMRNYHQ---PELLSFARVQELGRA-YEHAHPGFFEQQVAAGSPDDVAIIL 173 I D+ +G+ Y +L++F V LGR Y ++ + PD V + Sbjct: 148 ---IVDEAKGLHQYRGMVGSKLVTFGDVMRLGRKLYSDGGDQQVKKLMGELEPDMVCGLF 204 Query: 174 YTSGTTGKPKGVCHSHRAMITTATTLVEFDHLDENDDILCYLPLAWVGDFLYSFAQQHVA 233 TSGTTG PK S + M+ A L D + E+ + + +LP AW+G+ + S + Sbjct: 205 TTSGTTGLPKLAMISFKNMLAMAHQLNTVDPVREDWEYVSFLPTAWIGEQMMSIPYHMIG 264 Query: 234 GFCLNCPESPNTVMTDLREIGPTYYFAPPRVYENILTQVMIRIEDAGWIKRKMFHGFMKV 293 GF +N PE+P+T+ D REI P + F+PPRV+E I +M R++DA IKR F K+ Sbjct: 265 GFKVNFPETPHTLWRDFREIAPHFMFSPPRVWERIAKDIMARVDDADPIKRAAFRLAYKI 324 Query: 294 ----ARRVGMRILDGKPGVSLVDRLQYAIGDLLVYGPLKNVLGMSRLRVAYTGGEAIGPD 349 ARR R++ G+ + R Y + L + + G+ R+ AYTGG IG D Sbjct: 325 GYEAARR---RLVKGRSRPPTLWRALYYLAYWLALRAVLDKAGLKRIVRAYTGGAMIGED 381 Query: 350 LFDFYRSLGINLKQLYGMTETCVTVCMQPSGDVKLDSVGRPMKGVEVRIDANGEVLVRSP 409 FY SLG+NLKQ+YG TE + P DV+ D+VG+P+ EV+I +GE+L+RSP Sbjct: 382 YVIFYHSLGVNLKQIYGQTEVAGIAVVHPDDDVRPDTVGKPLPLTEVKIAEDGEILMRSP 441 Query: 410 GLMKEYFKRPDATAEAIDQDGYFHTGDAGFFDSDGHLKIIDRAKDVGKMACGSMFAPKYI 469 +MK Y+K +ATA+ I DG+ TGD G DGHLK++DRAK++ ++ G+ AP+ I Sbjct: 442 AMMKGYYKNEEATAKTI-VDGWLRTGDVGVLTEDGHLKVLDRAKEIIILSDGTKVAPQVI 500 Query: 470 ENKLKFFPFIKEAVTFGNGREQCMAFINIDMDAVGNWAERRNLPYSGYTDLAANPAVYDL 529 +NKLKF P+I EA G+G+ A +NID + V WAERR +P++ Y+DL+ P V L Sbjct: 501 QNKLKFSPYIGEAAIVGSGKPYLAALVNIDFEIVSRWAERRRIPFTSYSDLSQKPEVLQL 560 Query: 530 VRECVEKVNADLVADPLLADSQIHRFLILHKELDPDDEELTRTRKVRRGFIAEKYAVLIE 589 ++ +EKVN L +I RF+ L KE PDDEE+TRTRK+RR I +YA LIE Sbjct: 561 LKREIEKVNRRLP-----EKERIRRFVSLFKEFHPDDEEMTRTRKLRRMVIESRYAGLIE 615 Query: 590 ALYAGHASQYIETQVKFEDGRQGMISADLRIADAK 624 A+Y+G + +K EDGR+ ++ ++I D + Sbjct: 616 AIYSGDEEYELTVTMKLEDGRRVQVTRRVKIIDVE 650 >PAE2464_1 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Pya Pyrobaculum aerophilum Length = 448 Score = 328 bits (841), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 165/400 (41%), Positives = 244/400 (61%), Gaps = 4/400 (1%) Query: 30 ASSFACGLAALGFKRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQDAPAADMAYVLA 89 S FA GL ALGF+RG LA++G N P + A +A ALGG+ V +Y D + AY L Sbjct: 50 VSKFAYGLLALGFRRGDHLAVVGYNRPEILIAEQSAMALGGISVGVYPDTLPEERAYWLD 109 Query: 90 DANVDFVMAEDQEQVDKVFEIKENLPRIAHVIYDDERGMRNYH---QPELLSFARVQELG 146 +V V+AEDQEQVDK+ +K++LP++ ++IY DER M Y +P LLS+ V++LG Sbjct: 110 YTDVKVVVAEDQEQVDKILVVKKDLPKLEYIIYWDERMMWQYKDIKEPGLLSWKEVEKLG 169 Query: 147 RAYEHAHPGFFEQQVAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVEFDHLD 206 + E+ +A PDDV +IL TSGTTG+PKGV ++ +M++ A L E D + Sbjct: 170 EEVRKSDSKLLEELIAKTHPDDVCLILSTSGTTGRPKGVMLTYSSMMSMAKNLWEVDPIQ 229 Query: 207 ENDDILCYLPLAWVGDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPTYYFAPPRVYE 266 EN + + YLP W+G+ + S A + GF +N E P T D REI P + F P RVYE Sbjct: 230 ENFEYVSYLPFGWIGEQMMSLAMHMLVGFKINFVEEPETFWRDFREIAPHFMFGPARVYE 289 Query: 267 NILTQVMIRIEDAGWIKRKMFHGFMKVARRVGMRILDGKPGVSLVDRLQYAIGDLLVYGP 326 I +++M +ED +K+F + + R L GK + L + I +L+Y Sbjct: 290 MIYSKIMEHLEDTWDFDKKIFEIALNIGYRKVEAELTGK-RIPFWLTLLWHISRMLIYRS 348 Query: 327 LKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLGINLKQLYGMTETCVTVCMQPSGDVKLDS 386 + + G+ R++ AY GG +GPD FYR+LG+NLK+++GMTE + P +V+LD+ Sbjct: 349 ILDKTGLKRIKYAYVGGSFLGPDYLKFYRALGVNLKRIWGMTEVSGIATVHPDNEVRLDT 408 Query: 387 VGRPMKGVEVRIDANGEVLVRSPGLMKEYFKRPDATAEAI 426 VG+P+ E++I +GE+LVR+P +M Y+KRP+ATAEA+ Sbjct: 409 VGKPIANTEIKIAEDGEILVRTPAVMAGYYKRPEATAEAL 448 >PM0925 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Pmu Pasteurella multocida Length = 606 Score = 260 bits (665), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 181/587 (30%), Positives = 291/587 (49%), Gaps = 49/587 (8%) Query: 32 SFACGLAALGFKRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQDAPAADMAYVLADA 91 +F+ L A +AI +N PR A + + VP+Y + A + Y+L +A Sbjct: 47 TFSYALLANHIGVQDKIAIFAHNMPRWTIADIGTMQVRAVVVPIYATSAAKQVEYILNNA 106 Query: 92 NVDFVMAEDQEQVDKVFEIKENLPRIAHVI-YDDERGMRNY-----HQPELLSFARVQEL 145 +V + DQE+ + EI + P+I ++ D ++N+ Q LL + +Q+ Sbjct: 107 DVKILFVGDQEEYNCTLEIIDACPQIQKIVAMKDNLDLKNHPKACDWQTFLLEGSPLQQT 166 Query: 146 GRAYEHAHPGFFEQQVAAGSPDDVAIILYTSGTTGKPKGV-------CHSHRAMITTATT 198 ++++ D+ ++YTSGTTG+PKGV H +A TT Sbjct: 167 A----------LQERLEQKQLTDLFTLIYTSGTTGEPKGVMLDYANLAHQLKAHDEAFTT 216 Query: 199 LVEFDHLDENDDILCYLPL------AWVGDFLYSFAQQHVAGFCLNC-PESPNTVMTDLR 251 L ++ + D L +LPL AWV L+ A +NC E N V L Sbjct: 217 L----NVSQYDSSLSFLPLSHIFERAWVAYVLHRGA--------VNCYLEDTNRVREALS 264 Query: 252 EIGPTYYFAPPRVYENILTQVMIRIEDAGWIKRKMFHGFMKVARRVGMRILDGKPGVSLV 311 EI PT A PR YE I T V +++ A +R +F+ + V ++ I KP + V Sbjct: 265 EIRPTLMCAVPRFYEKIYTAVWDKVQKAPLFRRMIFNWAIAVGQKRFQFISQKKP-IPFV 323 Query: 312 DRLQYAIGDLLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLGINLKQLYGMTETC 371 R QYA+ D LV L+ +LG R+R+ GG + P++ F+ S+GIN+K YGMTET Sbjct: 324 LRQQYALADKLVLSKLRQLLG-GRIRMMPCGGAKLEPNIGLFFHSIGINVKLGYGMTETT 382 Query: 372 VTVCMQPSGDVKLDSVGRPMKGVEVRIDANGEVLVRSPGLMKEYFKRPDATAEAIDQDGY 431 TV G + +S+G M G EV+I N E+LVR +M+ Y+K+P TA++ +DG+ Sbjct: 383 ATVSCWEEGHFEPNSIGTLMPGAEVKIGENNEILVRGGMVMRGYYKKPQETADSFTEDGF 442 Query: 432 FHTGDAGFFDSDGHLKIIDRAKDVGKMACGSMFAPKYIENKLKFFPFIKEAVTFGNGREQ 491 TGDAG FD G+L I DR K++ K + G AP+YIE K+ FI++ + ++ Sbjct: 443 LKTGDAGEFDPQGNLYITDRIKELMKTSNGKYIAPQYIETKVGKDKFIEQIAVIADAKKY 502 Query: 492 CMAFINIDMDAVGNWAERRNLPYSGYTDLAANPAVYDLVRECVEKVNADLVADPLLADSQ 551 A I +++ +A++ N+ Y +L + + + + + + +L + Q Sbjct: 503 VSALIVPCFNSLEEYAKQLNIKYHDRLELIKHSDILQMFEQRINDLQKELP-----SFEQ 557 Query: 552 IHRFLILHKELDPDDEELTRTRKVRRGFIAEKYAVLIEALYAGHASQ 598 I +F +L + EE+T T K+RR I E+Y IEA+Y + + Sbjct: 558 IKKFTLLPQAFTTKMEEITPTLKLRRKVILERYKAQIEAMYKEYTHK 604 >YPO0537 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Ype Yersinia pestis Length = 601 Score = 256 bits (654), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 170/570 (29%), Positives = 284/570 (49%), Gaps = 22/570 (3%) Query: 30 ASSFACGLAALGFKRGMSLAIIGNNCPRLYWAMS--AAQALGGMPVPLYQDAPAADMAYV 87 + + L +LG + I NN + W++ A L G+ VPLY AA AY+ Sbjct: 47 VTRISTALLSLGAAIQERIGIFANNS--MAWSLVDLAILQLRGVSVPLYATNTAAQAAYI 104 Query: 88 LADANVDFVMAEDQEQVDKVFEIKENLPRIAHVIYDDE----RGMRNYHQPELLSFARVQ 143 + DA+V + +Q Q D ++ P++AH+I D RG Y Q L F + Q Sbjct: 105 VNDADVRILFVGEQSQFDIAITLQPLCPQLAHIIVLDPAVDLRGC-EYAQ-HLADFEQQQ 162 Query: 144 ELGRAYEHAHPGFFEQQVAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVEFD 203 + +H ++ DD+ ++YTSGTTG+PKGV +R M + Sbjct: 163 QPDTVQQH----LLTTRIEGCDLDDLFTLIYTSGTTGEPKGVMLDYRNMAAQLYLHDQRL 218 Query: 204 HLDENDDILCYLPLAWVGDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPTYYFAPPR 263 L D L +LPL+ V + +SF H + + + V + ++ + PT A PR Sbjct: 219 TLTPQDVSLSFLPLSHVFERAWSFYVMHTGAQNVYI-RNTDWVRSAMQAVKPTVMCAVPR 277 Query: 264 VYENILTQVMIRIEDAGWIKRKMFHGFMKVARRVGMRILDGKPGVSLVDRLQYAIGDLLV 323 YE + + + ++ A W +R +FH + R R+ G+P L +++ Y + D LV Sbjct: 278 FYEKVFSAINDKVALAKWHQRVLFHWAVGCGERKFKRLQQGRPLPWLSEQM-YTLADRLV 336 Query: 324 YGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLGINLKQLYGMTETCVTVCMQPSGDVK 383 L+ VLG R+R G + ++ F++++G+N+K YGMTETC TV + + Sbjct: 337 LRKLRGVLG-GRVRFLPAAGARLDDNIILFFQAIGVNIKYGYGMTETCATVSCWEEQNFR 395 Query: 384 LDSVGRPMKGVEVRIDANGEVLVRSPGLMKEYFKRPDATAEAIDQDGYFHTGDAGFFDSD 443 S+G+P+ G++VR+ A E+ VR P +M+ YFK+P T EA +DG+ TGDAG D+ Sbjct: 396 FGSIGKPLPGIDVRLGAENEIQVRGPIVMRGYFKKPQETLEAFTEDGWLKTGDAGALDAQ 455 Query: 444 GHLKIIDRAKDVGKMACGSMFAPKYIENKLKFFPFIKEAVTFGNGREQCMAFINIDMDAV 503 GHL I +R KD+ K + G AP+ IE L FI++ + R+ A I +++ Sbjct: 456 GHLFITERLKDLMKTSGGKYIAPQMIEGTLGQDRFIEQVAIIADTRKFVSALIVPSFESL 515 Query: 504 GNWAERRNLPYSGYTDLAANPAVYDLVRECVEKVNADLVADPLLADSQIHRFLILHKELD 563 +A NL Y +L + + ++ + ++++ +L Q+ +F +L + Sbjct: 516 EEYARSINLKYHDRLELLRHSHIVNMFEQRLKEIQKELA-----LFEQVKKFTLLPQAFT 570 Query: 564 PDDEELTRTRKVRRGFIAEKYAVLIEALYA 593 + ELT T K+RR I ++Y I+ +Y+ Sbjct: 571 METGELTPTMKLRRKIILQRYQNEIDLMYS 600 >HI0002 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Hin Haemophilus influenzae Length = 607 Score = 244 bits (623), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 175/586 (29%), Positives = 284/586 (48%), Gaps = 25/586 (4%) Query: 14 QEGGKWWRRD---KTRKXSASSFACGLAALGFKRGMSLAIIGNNCPRLYWAMSAAQALGG 70 +E G W RD K + + + L A +AI +N R A + Sbjct: 35 KEHGLW--RDISWKNFQEQLNQLSRALLAHNIDVQDKIAIFAHNMERWTIVDIATLQIRA 92 Query: 71 MPVPLYQDAPAADMAYVLADANVDFVMAEDQEQVDKVFEIKENLPRIAHVIYDDERGMRN 130 + VP+Y A ++L A+V + DQEQ D+ EI + P++ ++ M++ Sbjct: 93 ITVPIYATNTAQQAEFILNHADVKILFVGDQEQYDQTLEIAHHCPKLQKIV-----AMKS 147 Query: 131 YHQPELLSFARVQE-LGRAYEHAHPGFFEQQVAAGSPDDVAIILYTSGTTGKPKGVCHSH 189 Q + + E + +A Q++ D+ I+YTSGTTG+PKGV + Sbjct: 148 TIQLQQDPLSCTWESFIKTGSNAQQDELTQRLNQKQLSDLFTIIYTSGTTGEPKGVMLDY 207 Query: 190 RAMITTATTLVEFDHLDENDDILCYLPLAWVGDFLYSFAQQHVAGFCLNCPESPNTVMTD 249 + T ++ + D L +LP + + + ++ H G L E N V + Sbjct: 208 ANLAHQLETHDLSLNVTDQDISLSFLPFSHIFERAWAAYILH-RGAILCYLEDTNQVRSA 266 Query: 250 LREIGPTYYFAPPRVYENILTQVMIRIEDAGWIKRKMFHGFMKVARRVGMRILD---GKP 306 L EI PT A PR YE I V+ +++ A +++ MFH A VG + D Sbjct: 267 LTEIRPTLMCAVPRFYEKIYAAVLDKVQKAPKLRQIMFHW----AISVGQKYFDLRANNK 322 Query: 307 GVSLVDRLQYAIGDLLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLGINLKQLYG 366 + + + Q+A+ D LV L+ +LG R+++ GG + P + F+ ++GIN+K YG Sbjct: 323 AIPFLLKKQFALADKLVLSKLRQLLG-GRIKMMPCGGAKLEPAIGLFFHAIGINIKLGYG 381 Query: 367 MTETCVTVCMQPSGDVKLDSVGRPMKGVEVRIDANGEVLVRSPGLMKEYFKRPDATAEAI 426 MTET TV +S+G M EV+I N E+LVR +MK Y+K+P+ TA+A Sbjct: 382 MTETTATVSCWHDFQFNPNSIGTLMPKAEVKIGENNEILVRGGMVMKGYYKKPEETAQAF 441 Query: 427 DQDGYFHTGDAGFFDSDGHLKIIDRAKDVGKMACGSMFAPKYIENKLKFFPFIKEAVTFG 486 +DG+ TGDAG FD G+L I DR K++ K + G AP+YIE+K+ FI++ Sbjct: 442 TEDGFLKTGDAGEFDEQGNLFITDRIKELMKTSNGKYIAPQYIESKIGKDKFIEQIAIIA 501 Query: 487 NGREQCMAFINIDMDAVGNWAERRNLPYSGYTDLAANPAVYDLVRECVEKVNADLVADPL 546 + ++ A I D++ +A++ N+ Y +L N D+++ ++NA V L Sbjct: 502 DAKKYVSALIVPCFDSLEEYAKQLNIKYHDRLELLKNS---DILKMFEHRINA--VQKEL 556 Query: 547 LADSQIHRFLILHKELDPDDEELTRTRKVRRGFIAEKYAVLIEALY 592 Q+ +F +L + E+T T K+RR I E+Y IEA+Y Sbjct: 557 AHFEQVKKFTLLSQAFSIKLGEITPTLKLRRKVILERYRKQIEAMY 602