BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0084.p (390 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc3159 [J] COG2890 Methylase of polypeptide chain release facto... 489 e-138 NMA1400 [J] COG2890 Methylase of polypeptide chain release facto... 321 2e-87 NMB1232 [J] COG2890 Methylase of polypeptide chain release facto... 300 5e-81 MJ0928 [J] COG2890 Methylase of polypeptide chain release factor... 68 4e-11 >RSc3159 [J] COG2890 Methylase of polypeptide chain release factors Rso Ralstonia solanacearum Length = 376 Score = 489 bits (1259), Expect = e-138, Method: Compositional matrix adjust. Identities = 253/382 (66%), Positives = 288/382 (75%), Gaps = 15/382 (3%) Query: 11 ITWTTAPTS--QQLRWHSEAGNPPPKRVVEADDQMAADAAYKLACEGTGLLWRGDFQNAR 68 ++WT A T+ RW SEAG PPPKRV ADD++ AD AY+ ACEG +LWRGDFQNAR Sbjct: 7 LSWTDAETATAHTARWRSEAGTPPPKRVTVADDRITADTAYRQACEGIAILWRGDFQNAR 66 Query: 69 QLLQALGRRVDRARERAQAKSKSKAAAAESPAQAFHHHRQMQAQRARILGMLLIPVDPGF 128 QLLQA+ RR DR +A A SP AF+ HRQ Q+QRAR LGMLLIP+D + Sbjct: 67 QLLQAMSRRADRKPPKA----------ATSPVDAFNLHRQAQSQRARTLGMLLIPLDADY 116 Query: 129 VVPLRRAPDVQAACREAYAPSLQDTSFAVSLRELQGLFGAHEWRKKGVEIGALG-SRIHP 187 +PLRRAPDV AC EAY + + VSLREL GL GAHEWRKKGV+I ALG +RIHP Sbjct: 117 TIPLRRAPDVHEACTEAYGAGEEPS--VVSLRELLGLIGAHEWRKKGVDIPALGGARIHP 174 Query: 188 WYGVFSPVRGEYVDLVAKAPLPSTELAFDIGVGSGVLSAVLARRGVRKIIATDMDPRALD 247 YGVFSPVRGEYVDLVAKAPLPS LAFDIG G+GVL+AVLA+RGV++++ TD D RAL Sbjct: 175 HYGVFSPVRGEYVDLVAKAPLPSQALAFDIGTGTGVLAAVLAKRGVKRVVGTDQDARALA 234 Query: 248 CARENLQGLGVLPQVELQEADLFPAGRAPLIVCNPPWLPARPSSPIEHAVYDPDSRMLLG 307 CA ENL LG+ QVE+ EADLFP GRAPLIVCNPPWLPARPSSPIE AVYDPDSRML G Sbjct: 235 CAHENLTRLGLQSQVEVIEADLFPEGRAPLIVCNPPWLPARPSSPIERAVYDPDSRMLRG 294 Query: 308 FLKGLPDHLTPQGEGWLILSDFAEHLGLRAPGMLAEAIAQAGLEVVARHDIKPRHGKAVD 367 FL GL HL P GEGWLILSDFAEHLGLR L I AGL+VV R D+KP+H KA D Sbjct: 295 FLDGLAAHLEPGGEGWLILSDFAEHLGLRTRDALLAMIEAAGLQVVGRDDVKPKHPKASD 354 Query: 368 RDDPLHAARSKETTSLYRLRAR 389 +DPL+ AR+ E TSL+RL+AR Sbjct: 355 ANDPLYQARAAEVTSLWRLKAR 376 >NMA1400 [J] COG2890 Methylase of polypeptide chain release factors NmA Neisseria meningitidis Z2491 Length = 331 Score = 321 bits (823), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 170/338 (50%), Positives = 223/338 (65%), Gaps = 10/338 (2%) Query: 50 KLACEGTGLLWRGDFQNARQLLQALGRRVDRARERAQAKSKSKAAAAESPAQAFHHHRQM 109 K A + +W+GDF NA+Q+L A+ ++V R+ A +S + AA FH HR Sbjct: 2 KAAYQNIATVWQGDFHNAKQVLSAMKKKV---RKPAAVRSDADIAAL------FHAHRMK 52 Query: 110 QAQRARILGMLLIPVDPGFVVPLRRAPDVQAACREAYAPSLQDTSFAVSLRELQGLFGAH 169 QAQ++RIL ML + + PGFV+ +RAPD++AA + Y + F + L L G GAH Sbjct: 53 QAQQSRILNMLAVEIRPGFVLDNKRAPDIRAALLDVYGEA-DGKPFFLPLNLLLGFMGAH 111 Query: 170 EWRKKGVEIGALGSRIHPWYGVFSPVRGEYVDLVAKAPLPSTELAFDIGVGSGVLSAVLA 229 EW KKGV + LG IH +GVFSP+RGEY+DL+A AP + AFDIG GSGVL+A+LA Sbjct: 112 EWHKKGVAVPQLGGSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILA 171 Query: 230 RRGVRKIIATDMDPRALDCARENLQGLGVLPQVELQEADLFPAGRAPLIVCNPPWLPARP 289 ++G+ +I TD +P+A+ CAR N+ LG QVE++E DLFP G A LIVCNPPWLPA+P Sbjct: 172 KQGIPSVIGTDTNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKP 231 Query: 290 SSPIEHAVYDPDSRMLLGFLKGLPDHLTPQGEGWLILSDFAEHLGLRAPGMLAEAIAQAG 349 +S +E A+YDP+S ML FL+ P HL P GE LI+SD AEHL LR L +A AQAG Sbjct: 232 TSAVESALYDPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAG 291 Query: 350 LEVVARHDIKPRHGKAVDRDDPLHAARSKETTSLYRLR 387 L V KP H KA D ++PL AR++ETT LYRL+ Sbjct: 292 LRVADVLKTKPVHKKAADPNNPLAFARNRETTFLYRLK 329 >NMB1232 [J] COG2890 Methylase of polypeptide chain release factors Nme Neisseria meningitidis MC58 Length = 306 Score = 300 bits (767), Expect = 5e-81, Method: Compositional matrix adjust. Identities = 158/308 (51%), Positives = 204/308 (66%), Gaps = 7/308 (2%) Query: 80 RARERAQAKSKSKAAAAESPAQAFHHHRQMQAQRARILGMLLIPVDPGFVVPLRRAPDVQ 139 R R+ A +S + AA FH HR QAQ++RIL ML + + PGFV+ +RAPD++ Sbjct: 4 RVRKPAAVRSDADIAAL------FHAHRMKQAQQSRILNMLAVEIRPGFVLDNKRAPDIR 57 Query: 140 AACREAYAPSLQDTSFAVSLRELQGLFGAHEWRKKGVEIGALGSRIHPWYGVFSPVRGEY 199 +A + Y + F + L L G GAHEW KKGV + LG IH +GVFSP+RGEY Sbjct: 58 SALLDVYGEA-DGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLGGSIHVPFGVFSPLRGEY 116 Query: 200 VDLVAKAPLPSTELAFDIGVGSGVLSAVLARRGVRKIIATDMDPRALDCARENLQGLGVL 259 +DL+A AP + AFDIG GSGVL+A+LA++G+ +I TD +P+A+ CAR N+ LG Sbjct: 117 LDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTDTNPKAVACARANIARLGFE 176 Query: 260 PQVELQEADLFPAGRAPLIVCNPPWLPARPSSPIEHAVYDPDSRMLLGFLKGLPDHLTPQ 319 QVE++E DLFP G A LIVCNPPWLPA+P+S +E A+YDP+S ML FL+ P HL P Sbjct: 177 KQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALYDPESAMLAAFLRDAPKHLNPD 236 Query: 320 GEGWLILSDFAEHLGLRAPGMLAEAIAQAGLEVVARHDIKPRHGKAVDRDDPLHAARSKE 379 GE LI+SD AEHL LR L +A AQAGL V KP+H KA + DPL AR++E Sbjct: 237 GEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADMMKTKPKHKKAANPSDPLAFARTRE 296 Query: 380 TTSLYRLR 387 TT LYRL+ Sbjct: 297 TTFLYRLK 304 >MJ0928 [J] COG2890 Methylase of polypeptide chain release factors Mja Methanococcus jannaschii Length = 197 Score = 67.8 bits (164), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 45/153 (29%), Positives = 81/153 (52%), Gaps = 13/153 (8%) Query: 176 VEIGALGSRIHPWYGVFSPVRGEYVDLVAKAPLPSTELAFDIGVGSGVLSAVLARRGVRK 235 +EI + ++HP V+ P + L + + ++ +IGVG+G++S A++G +K Sbjct: 3 IEIEGIKLKLHPE--VYEPAEDSILLLKNLVDVKNKDV-LEIGVGTGLISIACAKKGAKK 59 Query: 236 IIATDMDPRALDCARENLQGLGVLPQVELQEADLFP--AGRAPLIVCNPPWLPARPSSPI 293 I+ D++P A+ A+EN + V + E+DLF G+ +I+ NPP+LP I Sbjct: 60 IVGVDINPYAVKLAKENAKLNNV--NISFFESDLFENVTGKFDVILFNPPYLPTSEDEKI 117 Query: 294 E---HAVYD--PDSRMLLG-FLKGLPDHLTPQG 320 + + +D D R +L F+ LP++L G Sbjct: 118 DSYLNFAFDGGKDGREILDRFIYELPNYLKKGG 150