BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0005.p (438 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1803 [P] COG0038 Chloride channel protein EriC Rso Ralstonia ... 504 e-142 BH0663 [P] COG0038 Chloride channel protein EriC Bha Bacillus ha... 303 6e-82 L105113 [P] COG0038 Chloride channel protein EriC Lla Lactococcu... 219 1e-56 SPy0723 [P] COG0038 Chloride channel protein EriC Spy Streptococ... 206 8e-53 CAC0442 [P] COG0038 Chloride channel protein EriC Cac Clostridiu... 176 8e-44 >RSc1803 [P] COG0038 Chloride channel protein EriC Rso Ralstonia solanacearum Length = 431 Score = 504 bits (1299), Expect = e-142, Method: Compositional matrix adjust. Identities = 274/419 (65%), Positives = 329/419 (78%) Query: 12 LLPYIVKWCLIASLIAALAGSASAFFLFALDYATHWRETHPWIIWLLPLGGFAVGWLYLK 71 +LPY+++W +ASL LAG SA L ALD+AT R HPW++WLLPL G AVG LY Sbjct: 1 MLPYLLRWLFLASLTGVLAGLGSAALLLALDWATLTRTAHPWLLWLLPLAGLAVGLLYHY 60 Query: 72 TGKPVEAGNNLLIDEIHDPKKVIPLRMAPLVLFGTVMSHLFGASVGREGTAVQMGGALAD 131 TG+ VE GNNLLIDEIHDPK+V+P RMAPL+L GTV++HLFG S GREGTAVQMGG+ AD Sbjct: 61 TGRAVEGGNNLLIDEIHDPKRVVPKRMAPLILLGTVVTHLFGGSAGREGTAVQMGGSFAD 120 Query: 132 QLTTVLRLRTEDRRILLMAGISAGFASVFGTPMAGALFGLEVLAIGRMRYDAILPCFVAA 191 LT + RL E RRILLM+GISAGFASVFGTP+AGA+FGLEVLAIGR+RYDAILPCFVAA Sbjct: 121 GLTRLFRLDNEARRILLMSGISAGFASVFGTPLAGAVFGLEVLAIGRLRYDAILPCFVAA 180 Query: 192 IAADQVGLAWGVHHTHYHMGGVVPVALWSVAAVIAAGVVFGLVGMVFANSTHRLSAFMKR 251 I D V GVHHT Y + V + ++ AV+ AG+VFGL GM FA THRL ++K+ Sbjct: 181 IVGDLVPPLLGVHHTPYAIPFVPHLTPLAIGAVVVAGIVFGLAGMTFAELTHRLGRWLKK 240 Query: 252 HVSYAPLRPFIGGAIVAVAVWALGTQRYIGLGIPVIVEAFQQPLAPWDFLGKMVFTVTSL 311 + + PLRP +GG +VA A ALGT +Y+GLGIPVIV+AF QPL +DF GK FT+ +L Sbjct: 241 RIPFGPLRPVLGGCVVAAAATALGTDKYLGLGIPVIVDAFHQPLPAYDFAGKAAFTIVTL 300 Query: 312 GTGFKGGEVTPLFYIGATVGNALAPLLNLPFSMLAGIGFVAVFAGAANTPIASTIMAVEL 371 +GFKGGEVTPLFYIGAT+GNAL +L LPF++LAG+GFVAVFAGAANTPIASTIMA+EL Sbjct: 301 ASGFKGGEVTPLFYIGATLGNALGYVLPLPFALLAGLGFVAVFAGAANTPIASTIMAIEL 360 Query: 372 FGPEIGPFAALACVVAYLFSGHTGIYHAQRVGQGKYLSLPEGLKIGEIGAYRARRQAER 430 FGPE+G FA +ACVV+YLFSGHTGIY AQRVG K S+PEGL++ E+ A+R A + Sbjct: 361 FGPEVGTFAGIACVVSYLFSGHTGIYPAQRVGHAKRASVPEGLRLSEVPAFRQASTAAK 419 >BH0663 [P] COG0038 Chloride channel protein EriC Bha Bacillus halodurans Length = 424 Score = 303 bits (775), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 163/427 (38%), Positives = 255/427 (59%), Gaps = 21/427 (4%) Query: 16 IVKWCLIASLIAALAGSASAFFLFALDYATHWRETHPWIIWLLPLGGFAVGWLYLKTG-- 73 ++ W +I A+ GSA+A L D+ RE PW + LPL G A+G+LY+K G Sbjct: 9 LLTWIFFGIMIGAIVGSATALLLTVNDHLGETRENRPWFVLFLPLAGLALGYLYMKAGTS 68 Query: 74 --KPVEAGNNLLIDEIHDPKKVIPLRMAPLVLFGTVMSHLFGASVGREGTAVQMGGALAD 131 + GNNL+I+ + K++ LR+ PLV GT M+ LFG S GREG A+QMGG++A+ Sbjct: 69 AGNELYKGNNLVIESVQGKGKML-LRLGPLVYLGTFMTILFGGSTGREGAAIQMGGSVAE 127 Query: 132 QLTTVLRLRTEDRRILLMAGISAGFASVFGTPMAGALFGLEVLAIGRMRYDAILPCFVAA 191 + + +++ D RILLM GISAGF + FGTP+ A+FG+E+ ++GR++++A++PC VA+ Sbjct: 128 AVNKLFKVKLIDTRILLMGGISAGFGAAFGTPITAAIFGMEMASLGRLKFEALVPCLVAS 187 Query: 192 IAADQVGLA-WGVHHTHYHMGGVVPVALWSVAAVIAAGVVFGLVGMVFA---NSTHRLSA 247 W V H + + V V+ + + VI +VF LV +++ + H+LS Sbjct: 188 FVGHYTTEKFWHVEHEKFIIATVPEVSALTFSKVILLAIVFSLVSVLYCQLRHGIHKLSE 247 Query: 248 --FMKRHVSYAPLRPFIGGAIVAVAVWALGTQRYIGLGIPVIVEAFQQPLAPWDFLGKMV 305 MK H +R F+GG I+ + +G+ Y G G+ ++ ++F Q + P+ FL K+V Sbjct: 248 KYTMKNHT----VRAFVGGLIIVALTYIIGSYDYNGRGLDMLEDSFTQDVPPYAFLAKLV 303 Query: 306 FTVTSLGTGFKGGEVTPLFYIGATVGNALAPLLNLPFSMLAGIGFVAVFAGAANTPIAST 365 FT ++G GF GGE PLF++GAT+GN L ++LP S LA +G + F G ANTPIA+ Sbjct: 304 FTAVTMGMGFVGGEAIPLFFVGATLGNTLHAFIDLPLSFLAALGMIVTFGGGANTPIAAF 363 Query: 366 IMAVELFGPEIGPFAALACVVAYLFSGHTGIYHAQRVGQGKYLSLPEGLKIGEIGAYRAR 425 ++ VE+F + F +AC+ +YLFSGH G++ +Q + + K S G++ GE + Sbjct: 364 LLGVEMFNGKGIEFFFVACLTSYLFSGHHGLWPSQTIYEPK--SRLYGVRKGE----TIK 417 Query: 426 RQAERDQ 432 R E + Sbjct: 418 RTEEMKE 424 >L105113 [P] COG0038 Chloride channel protein EriC Lla Lactococcus lactis Length = 403 Score = 219 bits (558), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 136/394 (34%), Positives = 222/394 (56%), Gaps = 16/394 (4%) Query: 17 VKWCLIASLIAALAGSASAFFLFALDYATHWRETHP-WIIWLLPLGGFAVGWLYLKTGKP 75 + + +A +I L G A F L Y T +R++H ++I L L G + ++Y GK Sbjct: 13 ILYVFLAVIIGGLIGIVDAIFGRGLIYITEFRDSHALYLIPFLTLAGLLIVYIYKNFGKD 72 Query: 76 VEAGNNLLIDEIHDPKKVIPLRMAPLVLFGTVMSHLFGASVGREGTAVQMGGALADQLTT 135 + G L+ + + ++ IP R+ PL++F T +SHLFGASVGREG AVQ+GG + + Sbjct: 73 SQKGMGLIFEAGNHGREDIPKRLVPLIVFSTWLSHLFGASVGREGVAVQIGGVIGHAIGK 132 Query: 136 VLRLRTEDRRILLMAGISAGFASVFGTPMAGALFGLEVLAIGRMRYDAILPCFVAAIAAD 195 L + E ++ILL+ G++AGFA +F TP+A F +E+L +G++ Y A++P V + A Sbjct: 133 KLSAK-EAKKILLITGMAAGFAGLFQTPIAATFFAIEILMLGKIEYRALIPALVGSYVAS 191 Query: 196 QVGLAWGVH------HTHYHMGGVVPVALWSVAAVIAAGVVFGLVGMVFANSTHRLSAFM 249 + G+ +T+ H+ P+ L +A + V FGLVG FA S + A + Sbjct: 192 WTSSSLGLEKFSFAINTNIHID---PLVLLKLAVI---AVCFGLVGRFFAESLAFMKATV 245 Query: 250 KRHVSYAPLRPFIGGAIVAVAVWALGTQRYIGLGIPVIVEAFQ-QPLAPWDFLGKMVFTV 308 + + R + G ++++ + + RY GLG +I +F + +D++ K++FTV Sbjct: 246 AKRIVNPYYRIILMGIVISIGLLVIHLDRYAGLGTNLISLSFNGGHINGYDWILKLIFTV 305 Query: 309 TSLGTGFKGGEVTPLFYIGATVGNALAPLLNLPFSMLAGIGFVAVFAGAANTPIASTIMA 368 S+ GF+GGEVTPLF IG+T+G ALA L LP +A G+V++F+ A N+ +A Sbjct: 306 LSISAGFQGGEVTPLFAIGSTLGAALAMLFGLPVEFVAAAGYVSIFSAATNSYFGPIFIA 365 Query: 369 VELFGPEIGPFAALACVVAYLFSGHTGIYHAQRV 402 E+FG + +AY+ +G++ IY AQ V Sbjct: 366 AEVFGFGSVQYILPIMTIAYVLNGNSSIY-AQEV 398 >SPy0723 [P] COG0038 Chloride channel protein EriC Spy Streptococcus pyogenes M1 GAS Length = 420 Score = 206 bits (524), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 129/337 (38%), Positives = 186/337 (55%), Gaps = 2/337 (0%) Query: 67 WLYLKTGKPVEAGNNLLIDEIHDPKKVIPLRMAPLVLFGTVMSHLFGASVGREGTAVQMG 126 +LY K GK V G L+ H K IP + PL+LF T ++HLFGAS GREG AVQ+G Sbjct: 68 FLYDKLGKEVRQGMGLVFQVGHGQKNQIPPMLIPLILFSTWVTHLFGASAGREGVAVQIG 127 Query: 127 GALADQLTTVLRLRTEDRRILLMAGISAGFASVFGTPMAGALFGLEVLAIGRMRYDAILP 186 ++ + + R +L+M G++AGFA +F TP+A +F LEVL +G +RY A+LP Sbjct: 128 ATISHYCRRFVTSQEAARHLLIM-GMAAGFAGLFQTPIAAVVFALEVLLVGTLRYSALLP 186 Query: 187 CFVAAIAADQVGLAWGVHHTHYHMGGVVPVALWSVAAVIAAGVVFGLVGMVFANSTHRLS 246 VAA A A G+ + + + ++ +I G++FGLVG FA Sbjct: 187 SLVAAYVASWTSHALGLEKFTIVLEETLTITPLTLVKLIGLGLIFGLVGNSFAYLLGWFK 246 Query: 247 AFMKRHVSYAPLRPFIGGAIVAVAVWALGTQRYIGLGIPVIVEAFQ-QPLAPWDFLGKMV 305 ++ + + R GA++++ + RY GLG +I AF Q + +D+L KM+ Sbjct: 247 PYLSQKLPNPYFRIAFIGALLSICLMIGHVGRYSGLGTNLIAAAFSGQTILTYDWLLKMI 306 Query: 306 FTVTSLGTGFKGGEVTPLFYIGATVGNALAPLLNLPFSMLAGIGFVAVFAGAANTPIAST 365 TV SL GF+GGEVTPLF IGA++G LAP L LP ++A +G+ VF A NT A Sbjct: 307 VTVISLSAGFQGGEVTPLFAIGASLGIVLAPYLGLPVLLVAALGYTTVFGSATNTFWAPI 366 Query: 366 IMAVELFGPEIGPFAALACVVAYLFSGHTGIYHAQRV 402 + +E+FGPE + AY+ S IY Q+V Sbjct: 367 FIGIEVFGPENALAYFVTSAAAYMVSHRHSIYSYQKV 403 >CAC0442 [P] COG0038 Chloride channel protein EriC Cac Clostridium acetobutylicum Length = 444 Score = 176 bits (447), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 123/395 (31%), Positives = 210/395 (53%), Gaps = 1/395 (0%) Query: 17 VKWCLIASLIAALAGSASAFFLFALDYATHWRETHPWIIWLLPLGGFAVGWLYLKTGKPV 76 +KW +++ +I L GS + F+ ++ +T + + LLP+G F + Sbjct: 19 LKWIILSIIIGCLVGSFTTLFVKLVNTSTSFAFKFKYYYLLLPIGLFFSNLIINLLPAAK 78 Query: 77 EAGNNLLIDEIHDPKKVIPLRMAPLVLFGTVMSHLFGASVGREGTAVQMGGALADQLTTV 136 G I +++ K + +++ P+ L T+++ +FG SVG EG A Q+GGA++ L + Sbjct: 79 GHGTEKAIKSVNEDKGNMDIKVVPVKLITTLITIIFGGSVGLEGPATQIGGAISSFLGSQ 138 Query: 137 LRLRTEDRRILLMAGISAGFASVFGTPMAGALFGLEVLAIGRMRYDAILPCFVAAIAADQ 196 +++ D+R L++ GISAGF SVF P+ A+F EVL IG++ Y +LP +++ + Sbjct: 139 IKMDVADKRRLVVCGISAGFVSVFNAPIGAAIFACEVLYIGKLSYITLLPSLISSFVSYY 198 Query: 197 VGLAWGVHHTHYHMGGVVPVALWSVAAVIAAGVVFGLVGMVFANSTHRLSAFMKRHVSYA 256 GL G + + V L + VI + G++ ++F ++ Y Sbjct: 199 TGLYLGGKPLRFPVSYVPKNQLKAFIGVIVFSFIVGILSILFIKLVKTTEFLFRKLKIYP 258 Query: 257 PLRPFIGGAIVAVAVWALGTQRYIGLGIPVIVEAFQ-QPLAPWDFLGKMVFTVTSLGTGF 315 PL+ IGG I+ + V ++ +G+G V A +P FL K + T +LG+G Sbjct: 259 PLKGVIGGIIIIILVTLTNSREALGIGDDVFTNAIMGTQTSPHLFLVKSLTTSLTLGSGG 318 Query: 316 KGGEVTPLFYIGATVGNALAPLLNLPFSMLAGIGFVAVFAGAANTPIASTIMAVELFGPE 375 GG +TP+ +IG+T+G+A A L+N + IG VA A +N PI+S ++++ELFG Sbjct: 319 SGGILTPMLFIGSTLGSAFAQLINSNLIFYSAIGMVAFLAACSNVPISSIVISIELFGST 378 Query: 376 IGPFAALACVVAYLFSGHTGIYHAQRVGQGKYLSL 410 IG +A++A ++Y+ GHT IY Q + K SL Sbjct: 379 IGLYASIAIGISYIIVGHTSIYPTQFIMTSKTPSL 413