BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0531.p (399 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 444 e-124 AGc4953 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 424 e-118 AGl2806 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 420 e-117 SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carbox... 419 e-117 PA2922 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 410 e-114 >PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Pae Pseudomonas aeruginosa Length = 406 Score = 444 bits (1142), Expect = e-124, Method: Compositional matrix adjust. Identities = 224/388 (57%), Positives = 280/388 (72%), Gaps = 2/388 (0%) Query: 12 VADLHDIHDEITGIRRHLHQHPELSFEEVDTAALVASRLEEWGYAVTRHIGGNGLVATLK 71 VA L ++ D++ +R+ +H HPEL FEE TAALVA L WGY V IG G+V L+ Sbjct: 8 VAWLEEVADDLRSLRQDIHAHPELGFEERRTAALVAECLRGWGYEVHEGIGRTGVVGVLR 67 Query: 72 AGTSARSIGLRADMDALPIHEETGLDWASVNPGAMHACGHDGHTAMLLGAAKHLARTRRF 131 G R +GLRADMDALPI E TGL ++S + G MHACGHDGHTAMLLGAA++LA TRRF Sbjct: 68 QGDGTRRLGLRADMDALPIVEATGLGYSSCHGGRMHACGHDGHTAMLLGAARYLAATRRF 127 Query: 132 DGTLHLIFQPAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEAGTFMFRSGPFMA 191 DGTL LIFQPAEE GA+ MLADGL ERFPCDA+FG+HN PG+EAG FR+GP MA Sbjct: 128 DGTLVLIFQPAEEG--QGGAEAMLADGLLERFPCDALFGMHNMPGLEAGHLGFRAGPMMA 185 Query: 192 ACDTVKIRLTGRGSHAARPHLSVDPVVAAASLVMALQTVVSRNVDPMETAVVTVGSLHAG 251 + D + + L G G H + PHLSVDP++AA+S VMALQ+VV+RNVDP + AVVTVG+L AG Sbjct: 186 SQDLLSVTLEGVGGHGSMPHLSVDPLLAASSAVMALQSVVARNVDPQKAAVVTVGALQAG 245 Query: 252 KASNVIPEFATLELSVRSFKPEVRALLEERIRALVALHAESYGARADIEYLRGYPVLVNS 311 +A+NVIP+ A L LS+R+ +VR + +R+R ++ L A SYG +A IE+ YPVLVNS Sbjct: 246 EAANVIPQRAVLRLSLRALDGQVREQVLQRVRQIIELQAASYGCQASIEHYPAYPVLVNS 305 Query: 312 DAETEFARSVAEELVGAEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNGQGQPMLHNAG 371 ETEFAR V EL GAE+V + GSEDFA+ LQ+ PG +L +GNG+G+PM+HN Sbjct: 306 VEETEFARQVGVELAGAEQVDGDTPKLMGSEDFAWMLQRCPGSYLFIGNGRGRPMVHNPA 365 Query: 372 YDFNDANIPVGAAFWTRLVERWLPVKAS 399 YDFND + GAA+W L E WL A+ Sbjct: 366 YDFNDDILVRGAAYWGALAETWLAAPAA 393 >AGc4953 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 393 Score = 424 bits (1089), Expect = e-118, Method: Compositional matrix adjust. Identities = 208/379 (54%), Positives = 261/379 (68%), Gaps = 4/379 (1%) Query: 21 EITGIRRHLHQHPELSFEEVDTAALVASRLEEWGYAVTRHIGGNGLVATLKAGTSARSIG 80 ++ IRRHLH+HPE+ E T+ +A +L E GY VTR + G G+VATL+ G S R++G Sbjct: 17 DVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATLRNGDSTRALG 76 Query: 81 LRADMDALPIHEETGLDWASVNPGAMHACGHDGHTAMLLGAAKHLARTRRFDGTLHLIFQ 140 +RAD+DALPIHEETG D+AS + G MHACGHDGHTAMLLGAAK +A R FDGTLHLIFQ Sbjct: 77 IRADIDALPIHEETGADYASTHQGVMHACGHDGHTAMLLGAAKIIAERRNFDGTLHLIFQ 136 Query: 141 PAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIRL 200 PAEE GA+ M+ DGLFERFPCDAVF +HN PG+ G F+ R GP +AA D KI + Sbjct: 137 PAEENF--GGARIMIEDGLFERFPCDAVFALHNDPGLPFGQFVLRDGPILAAVDECKITV 194 Query: 201 TGRGSHAARPHLSVDPVVAAASLVMALQTVVSRNVDPMETAVVTVGSLHAGKASNVIPEF 260 G G H A P + DP+VA AS++MALQTVVSRN+ P +AVVTVG+ HAG ASNVIPE Sbjct: 195 RGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGMASNVIPET 254 Query: 261 ATLELSVRSFKPEVRALLEERIRALVALHAESYGARADIEYLRGYPVLVNSDAETEFARS 320 A + L++RSF P VR LE+RIRA+ A SYG I+Y RGY VN AET++ Sbjct: 255 AEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHKAETDYVAD 314 Query: 321 VAEELVGAEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNGQ--GQPMLHNAGYDFNDAN 378 +A G EKV+ P G+EDFAY L++RPGC+ +G + P LH+ +DFND Sbjct: 315 LARRFAGPEKVLEMKRPSMGAEDFAYMLEKRPGCYFFLGTARTDNDPPLHHPKFDFNDEI 374 Query: 379 IPVGAAFWTRLVERWLPVK 397 +P+G FW L E +L + Sbjct: 375 LPIGTTFWVDLAEDYLKAR 393 >AGl2806 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 422 Score = 420 bits (1079), Expect = e-117, Method: Compositional matrix adjust. Identities = 212/375 (56%), Positives = 263/375 (70%), Gaps = 3/375 (0%) Query: 20 DEITGIRRHLHQHPELSFEEVDTAALVASRLEEWGYAVTRHIGGNGLVATLKAGTSARSI 79 +E IR LH+HPELS +E TAAL+A L +GY VT +GG+G+VATLK G + + Sbjct: 50 EESIAIRHDLHRHPELSLKETRTAALIARYLLSFGYEVTEGVGGHGVVATLKRGHGEKRL 109 Query: 80 GLRADMDALPIHEETGLDWASVNPGAMHACGHDGHTAMLLGAAKHLARTRRFDGTLHLIF 139 G+RAD+DALPIHEETGL +AS G MHACGHDGHT +LL AA++LA T F GTL LIF Sbjct: 110 GIRADIDALPIHEETGLAYASETSGIMHACGHDGHTTVLLAAARYLAETSNFSGTLTLIF 169 Query: 140 QPAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIR 199 QPAEE G +GA+ M+ D LFERFP DAVFG+HN PGVE G F F GP MA+ D +R Sbjct: 170 QPAEEIG--AGARAMIKDRLFERFPVDAVFGLHNWPGVETGQFGFVEGPAMASVDKASVR 227 Query: 200 LTGRGSHAARPHLSVDPVVAAASLVMALQTVVSRNVDPMETAVVTVGSLHAGKASNVIPE 259 + G+G H A PH +VDPV+A+AS + ALQ++VSRN+DP E AV+TVGS+H G ASNVIPE Sbjct: 228 IIGKGGHGAAPHETVDPVLASASFITALQSIVSRNIDPREMAVLTVGSIHGGTASNVIPE 287 Query: 260 FATLELSVRSFKPEVRALLEERIRALVALHAESYGARADIEYLRGYPVLVNSDAETEFAR 319 L+++ R+F VR LEER+ AL AES+GA AD+ Y RG+P ++N ET FAR Sbjct: 288 SVELQITARAFSTAVRDRLEERLPALAKAQAESFGATADVSYSRGFPPVINHAPETAFAR 347 Query: 320 SVAEELVGAEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNGQGQPMLHNAGYDFNDANI 379 VA A ++ F P SEDFA+ LQ+RPG +L VGNG+ P LH+ YDFNDA I Sbjct: 348 DVARRHFPAAQIADAFQPRTASEDFAFMLQERPGSYLFVGNGESAP-LHSPHYDFNDAII 406 Query: 380 PVGAAFWTRLVERWL 394 P A FW RL E +L Sbjct: 407 PPAARFWVRLAEAYL 421 >SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Sme Sinorhizobium meliloti Length = 389 Score = 419 bits (1077), Expect = e-117, Method: Compositional matrix adjust. Identities = 207/377 (54%), Positives = 259/377 (68%), Gaps = 6/377 (1%) Query: 21 EITGIRRHLHQHPELSFEEVDTAALVASRLEEWGYAVTRHIGGNGLVATLKAGTSARSIG 80 E+ IRR LH HPEL EE T+A +A LEE GY V I G+V TL+ GT +RSIG Sbjct: 13 ELVAIRRDLHAHPELGLEETRTSAFIARHLEELGYEVATGIAKTGVVGTLRNGTGSRSIG 72 Query: 81 LRADMDALPIHEETGLDWASVNPGAMHACGHDGHTAMLLGAAKHLARTRRFDGTLHLIFQ 140 +RAD+DALPI EETG+ +AS PG MHACGHDGHTAMLLGAA+ LA R FDGT+HLIFQ Sbjct: 73 IRADIDALPIQEETGVAYASTKPGLMHACGHDGHTAMLLGAARALAERRNFDGTIHLIFQ 132 Query: 141 PAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIRL 200 PAEE GA+ M+ +GLF+RFPCDAVF +HN P + G F R GP MAA D +I + Sbjct: 133 PAEENA--GGAKIMVDEGLFDRFPCDAVFALHNEPNLPFGQFALREGPIMAAVDEARITV 190 Query: 201 TGRGSHAARPHLSVDPVVAAASLVMALQTVVSRNVDPMETAVVTVGSLHAGKASNVIPEF 260 GRG H A P + DP+V AS+VMALQT+V+RN+ PM+ +VVTVG+ HAG ASN+IPE Sbjct: 191 HGRGGHGAEPQATADPIVCGASIVMALQTIVARNIHPMDPSVVTVGAFHAGSASNIIPER 250 Query: 261 ATLELSVRSFKPEVRALLEERIRALVALHAESYGARADIEYLRGYPVLVNSDAETEFARS 320 A + + +RSF P VR LE RIR + A S+G RA ++Y R Y +N AET+F R Sbjct: 251 AEIVVGIRSFDPAVRDELERRIRMIAEAQASSFGMRATVDYERSYDATINHKAETDFLRE 310 Query: 321 VAEELVGAEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGN---GQGQPMLHNAGYDFNDA 377 A GA+KV+ P GSEDFAY L++RPG + +G+ G+ + LH+ GYDFND Sbjct: 311 AAIRFAGADKVVDLARPFMGSEDFAYMLKERPGSYFFLGSRVTGE-EKSLHHPGYDFNDD 369 Query: 378 NIPVGAAFWTRLVERWL 394 +P+GAAFWT L E +L Sbjct: 370 LLPIGAAFWTELAEAYL 386 >PA2922 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Pae Pseudomonas aeruginosa Length = 389 Score = 410 bits (1055), Expect = e-114, Method: Compositional matrix adjust. Identities = 211/379 (55%), Positives = 263/379 (69%), Gaps = 5/379 (1%) Query: 18 IHDEITGIRRHLHQHPELSFEEVDTAALVASRLEEWGYAVTRHIGGNGLVATLKAGTSAR 77 + +E+ +RRH+H HPEL FEE TAALVA LE WGY V+R +G G+V TL G Sbjct: 13 LREEMVTLRRHIHAHPELGFEERRTAALVAECLEAWGYQVSRGVGRTGVVGTLCRGEGP- 71 Query: 78 SIGLRADMDALPIHEETGLDWASVNPGAMHACGHDGHTAMLLGAAKHLARTRRFDGTLHL 137 ++GLRADMDALPI E TGL +AS G MHACGHDGHTAMLL AA+HL + + GTL L Sbjct: 72 ALGLRADMDALPIQEATGLPYASQVDGVMHACGHDGHTAMLLAAARHLVESPHWRGTLQL 131 Query: 138 IFQPAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEAGTFMFRSGPFMAACDTVK 197 IFQPAEE GA+ ML DGL ERFPCDA+F +HN PG G F GPFMA+ DTV Sbjct: 132 IFQPAEEG--LGGARAMLDDGLLERFPCDAIFAMHNVPGYPVGHLGFHPGPFMASADTVV 189 Query: 198 IRLTGRGSHAARPHLSVDPVVAAASLVMALQTVVSRNVDPMETAVVTVGSLHAGKASNVI 257 IR+ G G H A P +VDPVV +++V+ALQ++VSRNVDP +TA+V+VG++HAG SNVI Sbjct: 190 IRVIGSGGHGAVPQRTVDPVVVCSAIVLALQSIVSRNVDPQDTAIVSVGAIHAGTVSNVI 249 Query: 258 PEFATLELSVRSFKPEVRALLEERIRALVALHAESYGARADIEYLRGYPVLVNSDAETEF 317 P A + LSVR+ E RALLE RI L A S+GARA+++Y +PVLVN +T F Sbjct: 250 PASAEMILSVRALTAETRALLERRIGELARGQAASFGARAEVDYRHCHPVLVNHPGQTAF 309 Query: 318 ARSVAEELVGAEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNGQGQP--MLHNAGYDFN 375 AR VA + +G E +I P SEDFA+ L++ PG +L +GNGQG+ LHN GYDFN Sbjct: 310 AREVARDWLGEECLIDGLRPFTASEDFAFILERCPGSYLVIGNGQGESGCQLHNPGYDFN 369 Query: 376 DANIPVGAAFWTRLVERWL 394 D + VGA++W RL ER+L Sbjct: 370 DDCLAVGASYWVRLAERFL 388