BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0298.p (261 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1147 [R] COG1235 Metal-dependent hydrolases of the beta-lacta... 268 1e-71 PA1012 [R] COG1235 Metal-dependent hydrolases of the beta-lactam... 238 8e-63 lin0319 [R] COG1235 Metal-dependent hydrolases of the beta-lacta... 163 3e-40 BH4023 [R] COG1235 Metal-dependent hydrolases of the beta-lactam... 161 1e-39 CAC3538 [R] COG1235 Metal-dependent hydrolases of the beta-lacta... 152 6e-37 >RSc1147 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Rso Ralstonia solanacearum Length = 261 Score = 268 bits (684), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 138/260 (53%), Positives = 177/260 (68%), Gaps = 6/260 (2%) Query: 1 MKFASLGSGSEGNALLISAAAGDQRPSSGTTVMLDCGFTLREAEKRMLRLGLDPSDVSGI 60 ++FASLGSGS+GNALL+ + G ++ T ++LDCGF +RE +R+ RLG P+ + I Sbjct: 3 VRFASLGSGSDGNALLVESTDG----TTSTRILLDCGFGIRETVRRLERLGCAPASLDAI 58 Query: 61 VVTHEHSDHVSGVFKFARRYRIPVWLSYGTWQAVAE--QANDVDVRFCADGQKLAIGDLE 118 +VTHEH DH+ + FA +Y +PVW S+GT++A A A+ VRFC +IGDL Sbjct: 59 LVTHEHGDHIGSAYAFAAKYSVPVWTSHGTYRATASLRGADRAAVRFCRADDAFSIGDLR 118 Query: 119 LSPYTVPHDAREPVQYVASDGDRRLGVLTDAGQPTAHLVQALDACDALLLECNHDRDMLD 178 + PYTVPHDAREP+Q++ DG RRLGVLTDAG T +L L A +LECNHDR+ML Sbjct: 119 ILPYTVPHDAREPLQFIFDDGARRLGVLTDAGMSTEYLCGHLTGLHAFVLECNHDREMLA 178 Query: 179 RSPYPAFLKRRIGGDYGHLANDASAEILRLLDKSRLKRVIGAHLSQQNNTPELARAAIES 238 S YP LK+RIGGD+GHLAND +A IL + L V+ AHLS+QNNTPELA AAI S Sbjct: 179 NSAYPWSLKQRIGGDFGHLANDIAAAILARVRHDGLHTVVAAHLSKQNNTPELAAAAIAS 238 Query: 239 VLGTTFGEIGIACQEEGFAW 258 LG G++ IA QE G W Sbjct: 239 ALGVPAGDVPIADQEAGLGW 258 >PA1012 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Pae Pseudomonas aeruginosa Length = 252 Score = 238 bits (608), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 124/261 (47%), Positives = 166/261 (63%), Gaps = 10/261 (3%) Query: 1 MKFASLGSGSEGNALLISAAAGDQRPSSGTTVMLDCGFTLREAEKRMLRLGLDPSDVSGI 60 M+FA LGSGS GN+ L+S SS T +++DCGF LRE KR+ RLGL P+ + I Sbjct: 1 MRFAVLGSGSRGNSALVS--------SSDTRILIDCGFPLREIIKRLARLGLSPAQLDAI 52 Query: 61 VVTHEHSDHVSGVFKFARRYRIPVWLSYGTWQAVAEQANDVDVRFCADGQKLAIGDLELS 120 +VTHEH+DH+ GV AR Y+IPV+LS GT + + V+ GQ+LAI LE+ Sbjct: 53 LVTHEHADHIHGVELLARHYQIPVYLSAGTLSGMRRPVHPVE--LLRAGQRLAIRGLEVQ 110 Query: 121 PYTVPHDAREPVQYVASDGDRRLGVLTDAGQPTAHLVQALDACDALLLECNHDRDMLDRS 180 V HDAREP Q+V SDG RR G+LTD G TA +++ A DAL++E NHD ML Sbjct: 111 VVGVSHDAREPTQFVLSDGQRRFGMLTDLGVATAEVLECYRAVDALVVEANHDSTMLAGG 170 Query: 181 PYPAFLKRRIGGDYGHLANDASAEILRLLDKSRLKRVIGAHLSQQNNTPELARAAIESVL 240 PYP FLK R+GG GHL N SAE+++ LD R++ ++ AHLS++NN+P+LAR L Sbjct: 171 PYPYFLKARVGGSRGHLNNHQSAELVKQLDWGRMQHLVLAHLSEKNNSPQLARQTFVDTL 230 Query: 241 GTTFGEIGIACQEEGFAWLDV 261 G G + +A Q G W ++ Sbjct: 231 GCDPGWLQVADQHSGLDWREI 251 >lin0319 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Lin Listeria innocua Length = 276 Score = 163 bits (413), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 83/243 (34%), Positives = 143/243 (58%), Gaps = 11/243 (4%) Query: 1 MKFASLGSGSEGNALLISAAAGDQRPSSGTTVMLDCGFTLREAEKRMLRLGLDPSDVSGI 60 ++F+ L SGS GNA L+ GDQ+ +++DCG + ++ E ++G D +D+ I Sbjct: 15 IRFSILASGSSGNATLVET--GDQK------ILIDCGLSGKKMEGLFAQVGRDMNDLDAI 66 Query: 61 VVTHEHSDHVSGVFKFARRYRIPVWLSYGTWQAVAEQANDV--DVRFCADGQKL-AIGDL 117 ++THEHSDH+ G+ AR+Y++P++ + TW+A+ +V + +F D + + + G + Sbjct: 67 LITHEHSDHIKGLGVLARKYKLPIYANAKTWKAMDNMIGEVSSEQKFQFDMETVKSFGSM 126 Query: 118 ELSPYTVPHDAREPVQYVASDGDRRLGVLTDAGQPTAHLVQALDACDALLLECNHDRDML 177 ++ + V HDA EP+ Y+ G+++ ++TD G + + + DA L E NHD +ML Sbjct: 127 QVESFGVSHDAIEPMFYIFHKGNKKFVMITDTGYVSDRMKGHIAGADAYLFESNHDVEML 186 Query: 178 DRSPYPAFLKRRIGGDYGHLANDASAEILRLLDKSRLKRVIGAHLSQQNNTPELARAAIE 237 YP +KRRI GD GH++N+ +A + + + KR+ HLS+ NN ELAR ++ Sbjct: 187 RMGRYPWNVKRRILGDEGHVSNEDAAIAMSEVITDQTKRIYLGHLSKDNNMKELARMSVT 246 Query: 238 SVL 240 L Sbjct: 247 QTL 249 >BH4023 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Bha Bacillus halodurans Length = 264 Score = 161 bits (408), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 92/260 (35%), Positives = 144/260 (55%), Gaps = 22/260 (8%) Query: 1 MKFASLGSGSEGNALLISAAAGDQRPSSGTTVMLDCGFTLREAEKRMLRLGLDPSDVSGI 60 ++F+ L SGS GNAL + + +++D G + ++ ++ ++ DPS ++GI Sbjct: 3 LRFSVLASGSTGNALYVE--------TDKQKLLVDAGLSGKKMDELFQKIDCDPSQLNGI 54 Query: 61 VVTHEHSDHVSGVFKFARRYRIPVWLSYGTWQAVAEQANDV--DVRFCAD-GQKLAIGDL 117 +VTHEHSDH+ GV AR+Y +P++ + TWQA+ + + D RF + G+ DL Sbjct: 55 LVTHEHSDHIKGVGIVARKYNLPIYANAKTWQAMEKALGTLTTDQRFHFEIGEVKTFADL 114 Query: 118 ELSPYTVPHDAREPVQYVASDGDRRLGVLTDAGQPTAHLVQALDACDALLLECNHDRDML 177 E+ + V HDA EP+ YV ++L + TD G + + + ++ D L+ E NHD +ML Sbjct: 115 EVESFGVSHDAAEPMFYVFHHDGKKLVLATDMGYVSERIKKTIENADTLIFEANHDLNML 174 Query: 178 DRSPYPAFLKRRIGGDYGHLANDASAEILRLLDKSRLKRVIGAHLSQQNNTPELARAAIE 237 YP +KRRI D GH++N+ +A L + R +R+ AHLS NN ELAR +E Sbjct: 175 RMGRYPWNVKRRILSDVGHVSNEDAAYALAEVIGDRTERIYLAHLSLDNNMKELARLTVE 234 Query: 238 SVLGTTFGEIGIACQEEGFA 257 VL QE+G A Sbjct: 235 QVL-----------QEQGLA 243 >CAC3538 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Cac Clostridium acetobutylicum Length = 261 Score = 152 bits (385), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 87/240 (36%), Positives = 132/240 (55%), Gaps = 14/240 (5%) Query: 1 MKFASLGSGSEGNALLISAAAGDQRPSSGTTVMLDCGFTLREAEKRMLRLGLDPSDVSGI 60 MKF SL SGS GN++ ++ S + V++D G + + EK + + DP+++ GI Sbjct: 1 MKFCSLYSGSSGNSIFVA--------SDNSKVLIDAGLSGKTIEKALKEIKEDPNEIDGI 52 Query: 61 VVTHEHSDHVSGVFKFARRYRIPVWLSYGTWQAVAE---QANDVDVRFCADGQKLAIGDL 117 VTHEHSDH+ GV +R+Y IP++ + TW+A+ + D +++ + I D+ Sbjct: 53 FVTHEHSDHIKGVGVLSRKYNIPIYANELTWKAMMNSIGKIKDDNIKIITQ-DYIEIKDM 111 Query: 118 ELSPYTVPHDAREPVQYVASDGDRRLGVLTDAGQPTAHLVQALDACDALLLECNHDRDML 177 + Y +PHDA P Y G++ V TD G + + D +LLE NHD +ML Sbjct: 112 NILNYKIPHDAASPCGYKILSGNKSACVATDLGHFADEVKSKISDADVILLESNHDVEML 171 Query: 178 DRSPYPAFLKRRIGGDYGHLAN-DASAEILRLLDKSRLKRVIGAHLSQQNNTPELARAAI 236 PYP LKRRI + GHL+N D I+ + K + K ++G HLS+ NN PELA + Sbjct: 172 KFGPYPYPLKRRILSEVGHLSNEDCGKAIVDIAKKGKKKIILG-HLSRTNNYPELAYKTV 230