BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0256.p (242 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0510_1 [T] COG3300 MHYT domain (predicted integral membrane s... 193 4e-49 PA1727_1 [T] COG3300 MHYT domain (predicted integral membrane se... 166 6e-41 STM3388_1 [T] COG3300 MHYT domain (predicted integral membrane s... 131 2e-30 PA3311_1 [T] COG3300 MHYT domain (predicted integral membrane se... 124 1e-28 slr2098_1 [T] COG3300 MHYT domain (predicted integral membrane s... 103 4e-22 >RSc0510_1 [T] COG3300 MHYT domain (predicted integral membrane sensor domain) Rso Ralstonia solanacearum Length = 236 Score = 193 bits (490), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 106/158 (67%), Positives = 119/158 (75%), Gaps = 3/158 (1%) Query: 27 MLIANYDKPLVVVSLLVAILASYTALDMAERINTTSHLHSRAARFWLAGGAVAMGLGIWS 86 ML +Y LV+ SL VAILASYTALDMA RI RAA++WLAGGA AMGLGIWS Sbjct: 1 MLAGSYHPLLVLFSLCVAILASYTALDMAGRIAMA---RGRAAQWWLAGGACAMGLGIWS 57 Query: 87 MHFIGMLAFRLPIALGYDVWITTLSLLIAIATSGYALWIVTRSELPLRRLFGSALLMGTG 146 MHF+GMLAFRLPI LGYD WIT SLLIAIA S +ALW+V + LP RL G ALLMG G Sbjct: 58 MHFVGMLAFRLPIPLGYDTWITLASLLIAIALSAFALWLVCQRRLPWPRLAGGALLMGAG 117 Query: 147 IAGMHYTGMAAMRMTPGIDYDLVLFIASILIAVAASAA 184 +A MHYTGMAAMRM+PGI YD LF S++IA+ AS A Sbjct: 118 VASMHYTGMAAMRMSPGILYDPALFCLSVVIAMLASGA 155 Score = 59.3 bits (142), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 53/131 (40%), Positives = 71/131 (54%), Gaps = 12/131 (9%) Query: 31 NYDKPLVVVSLLVAILASYTALDMAERINTTSHLHSRAARFWLAGGAVAMGLGIWSMHFI 90 YD + + SLL+AI S AL + + R LAGGA+ MG G+ SMH+ Sbjct: 73 GYDTWITLASLLIAIALSAFALWLVCQ--------RRLPWPRLAGGALLMGAGVASMHYT 124 Query: 91 GMLAFRLPIALGYDVWITTLSLLIAIATSGYALWI----VTRSELPLRRLFGSALLMGTG 146 GM A R+ + YD + LS++IA+ SG ALWI +S G+A++MG Sbjct: 125 GMAAMRMSPGILYDPALFCLSVVIAMLASGAALWIAFRLRRQSRRRRAMRAGAAVVMGLA 184 Query: 147 IAGMHYTGMAA 157 I GMHYTGMAA Sbjct: 185 IVGMHYTGMAA 195 >PA1727_1 [T] COG3300 MHYT domain (predicted integral membrane sensor domain) Pae Pseudomonas aeruginosa Length = 237 Score = 166 bits (419), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 98/158 (62%), Positives = 118/158 (74%), Gaps = 3/158 (1%) Query: 27 MLIANYDKPLVVVSLLVAILASYTALDMAERINTTSHLHSRAARFWLAGGAVAMGLGIWS 86 MLI++Y++ LV SL+VAILASYTALDMA R+ R A WL GGA AMG GIWS Sbjct: 1 MLISSYNQVLVAFSLIVAILASYTALDMAGRVTLA---KGREALSWLIGGAFAMGFGIWS 57 Query: 87 MHFIGMLAFRLPIALGYDVWITTLSLLIAIATSGYALWIVTRSELPLRRLFGSALLMGTG 146 MHF+GMLAF LPI LGYD+ +T LSLL+A+ +S +ALW+V ++ELP +RL ALLMG+G Sbjct: 58 MHFVGMLAFSLPIPLGYDLGLTLLSLLLAVGSSAFALWLVCQAELPWQRLALGALLMGSG 117 Query: 147 IAGMHYTGMAAMRMTPGIDYDLVLFIASILIAVAASAA 184 IA MHYTGMAA+ M PGI YD + SILIAV AS A Sbjct: 118 IAAMHYTGMAALLMMPGIVYDPLWLGLSILIAVIASGA 155 Score = 63.9 bits (154), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 45/95 (47%), Positives = 56/95 (58%), Gaps = 4/95 (4%) Query: 73 LAGGAVAMGLGIWSMHFIGMLAFRLPIALGYDVWITTLSLLIAIATSGYALWIVTRSELP 132 LA GA+ MG GI +MH+ GM A + + YD LS+LIA+ SG ALWI R Sbjct: 107 LALGALLMGSGIAAMHYTGMAALLMMPGIVYDPLWLGLSILIAVIASGAALWIAFRLRHG 166 Query: 133 LRRLF----GSALLMGTGIAGMHYTGMAAMRMTPG 163 RR+ G+AL+MG I GMHYTGMAA + G Sbjct: 167 SRRIVLVRAGAALVMGCAIVGMHYTGMAAAQFPLG 201 >STM3388_1 [T] COG3300 MHYT domain (predicted integral membrane sensor domain) Sty Salmonella typhimurium LT2 Length = 262 Score = 131 bits (329), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 75/157 (47%), Positives = 106/157 (67%), Gaps = 3/157 (1%) Query: 27 MLIANYDKPLVVVSLLVAILASYTALDMAERINTTSHLHSRAARFWLAGGAVAMGLGIWS 86 M ++ Y+ LV VS VAI ASYTAL+MA R+ ++ + AR WL GG A+G+GIW+ Sbjct: 1 MPVSEYNHILVAVSFAVAIFASYTALNMAGRVAGSARSN---ARIWLMGGGFALGVGIWA 57 Query: 87 MHFIGMLAFRLPIALGYDVWITTLSLLIAIATSGYALWIVTRSELPLRRLFGSALLMGTG 146 MHF+GMLA + + +D ++T LS+LIAI +S +ALW+V+ +L LRRL AL+MG G Sbjct: 58 MHFVGMLAMDHAMNMRFDPFLTGLSMLIAIGSSLFALWLVSAEKLRLRRLLPGALVMGLG 117 Query: 147 IAGMHYTGMAAMRMTPGIDYDLVLFIASILIAVAASA 183 I+ MHYTGMAA++ I ++ SI+IA+ AS Sbjct: 118 ISAMHYTGMAALQFASIIVWNSAWVALSIIIALLASC 154 Score = 61.2 bits (147), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 52/133 (39%), Positives = 76/133 (57%), Gaps = 18/133 (13%) Query: 32 YDKPLVVVSLLVAILASYTALDM--AERINTTSHLHSRAARFWLAGGAVAMGLGIWSMHF 89 +D L +S+L+AI +S AL + AE++ R R L GA+ MGLGI +MH+ Sbjct: 74 FDPFLTGLSMLIAIGSSLFALWLVSAEKL--------RLRR--LLPGALVMGLGISAMHY 123 Query: 90 IGMLAFRLPIALGYDVWITTLSLLIAIATSGYALWIVTR-----SELPLRRLFGSALLMG 144 GM A + + ++ LS++IA+ S ALW+ R +++ LRR G+A+LMG Sbjct: 124 TGMAALQFASIIVWNSAWVALSIIIALLASCGALWLTFRLRHEGTDVALRRA-GAAVLMG 182 Query: 145 TGIAGMHYTGMAA 157 IAGMHY GM A Sbjct: 183 IAIAGMHYAGMKA 195 >PA3311_1 [T] COG3300 MHYT domain (predicted integral membrane sensor domain) Pae Pseudomonas aeruginosa Length = 313 Score = 124 bits (312), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 90/209 (43%), Positives = 110/209 (52%), Gaps = 23/209 (11%) Query: 5 TESPAFQRPSLNR------------RF---SQASLFAMLIANYDKPLVVVSLLVAILASY 49 T SP +RP + R RF S + + Y LV ++ LVA LA Y Sbjct: 39 TPSPHPRRPRVFRVQGDTAMDWQGLRFLGESPVDGYVLQNCTYSPSLVALAFLVACLAGY 98 Query: 50 TALDMAERI-NTTSHLHSRAARFWLAGGAVAMGLGIWSMHFIGMLAFRLPIALGYDVWIT 108 TALDM ER+ N+ SH R W GA +G GIW+ HF+ MLAF PIAL YD+ IT Sbjct: 99 TALDMVERVGNSLSH-----PRLWQWIGAFCLGSGIWATHFVAMLAFHAPIALRYDLPIT 153 Query: 109 TLSLLIAIATSGYALWIVTRSELPLRRLFGSALLMGTGIAGMHYTGMAAMRMTPGIDYDL 168 LSLLIA+A S +++ R L +A +G GIA MHYTGMAAMR Y Sbjct: 154 GLSLLIAVAASYLTMYMTARPRFGLLPCLLAACCIGLGIAAMHYTGMAAMRSVATQYYQP 213 Query: 169 VLFIASILIAVAASAANFLGXVPVGTRGR 197 LF S+LIA+ A A L VP RGR Sbjct: 214 SLFALSVLIAIGA-AFTALAAVPY-LRGR 240 >slr2098_1 [T] COG3300 MHYT domain (predicted integral membrane sensor domain) Syn Synechocystis Length = 260 Score = 103 bits (257), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 64/149 (42%), Positives = 88/149 (59%), Gaps = 2/149 (1%) Query: 31 NYDKPLVVVSLLVAILASYTALDMAERINTTSHLHSRAARFWLAGGAVAMGLGIWSMHFI 90 +YD LV++S+ +AI +Y A MA + SR L+G MGLGIW+MHFI Sbjct: 19 SYDPKLVLLSVAIAIFTAYMAFLMASSALSLPGRWSRKIALALSG--TVMGLGIWAMHFI 76 Query: 91 GMLAFRLPIALGYDVWITTLSLLIAIATSGYALWIVTRSELPLRRLFGSALLMGTGIAGM 150 GML F LP + YD IT +S++ AIA S YAL ++ + L A+ G GI M Sbjct: 77 GMLGFELPCPISYDPLITFISVIPAIAASIYALNFLSFQAPTAKNLMVGAIWFGLGIGTM 136 Query: 151 HYTGMAAMRMTPGIDYDLVLFIASILIAV 179 HY+GMAA+ + I Y+L LF+ S++IAV Sbjct: 137 HYSGMAALELHGNIYYELPLFVLSLVIAV 165