BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0106.p (345 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr4915 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 426 e-119 SMc01992 [ER] COG1063 Threonine dehydrogenase and related Zn-dep... 422 e-118 AGl1091 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 409 e-114 YLR070c [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 256 6e-68 BS_gutB [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 236 4e-62 >mlr4915 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Mlo Mesorhizobium loti Length = 348 Score = 426 bits (1096), Expect = e-119, Method: Compositional matrix adjust. Identities = 203/345 (58%), Positives = 261/345 (75%) Query: 1 MQALVLEATRELALRDIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMV 60 M+ALVLE EL+LR+I LP D+G DV+I IHTVG+CGSD+HYYTHG IG + V APMV Sbjct: 1 MRALVLEKKGELSLREIALPLDVGPDDVKIAIHTVGVCGSDVHYYTHGAIGSYVVRAPMV 60 Query: 61 LGHEASGTIIEVGSGVTQLKVGDRVCMEPGIPRLDSPATLRGLYNLDPSVRFWATPPIHG 120 LGHEA+GT++E G+ V K GDRVCMEPG+P L S AT G+YN+DP V FWATPP+HG Sbjct: 61 LGHEAAGTVVETGANVETFKAGDRVCMEPGVPNLSSRATKLGIYNVDPDVSFWATPPVHG 120 Query: 121 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 L VHPAAFTY+LPDNVSFAEGA+VEP +IG+QAA++AR+ PGD AVV+G G IG M Sbjct: 121 VLAPYAVHPAAFTYKLPDNVSFAEGAMVEPFAIGMQAASRARIVPGDVAVVVGCGPIGIM 180 Query: 181 TALAALAGGAARVILADVVAEKLAHFANNPAVITVDVTQQALTDVVQQVTEGWGADVVFE 240 ALAALAGG ++V+++D A KL A ++ V++ +++L D V T+ WGAD+VFE Sbjct: 181 IALAALAGGCSKVLISDFSAPKLKIAAQYAGIVPVNIGERSLVDAVAAATDKWGADIVFE 240 Query: 241 ASGHAGVYQTLLDLVCPGGCAVLVGMPPEPVGLDVVTMQTKEVRLESVFRYANIFPRALA 300 ASG + L D+V PGG VLVG+P EPV L+V +KEVR+E+VFRYANIF RAL Sbjct: 241 ASGSPKAFADLFDVVRPGGAVVLVGLPVEPVALNVPAAISKEVRIETVFRYANIFDRALQ 300 Query: 301 LLSSGMIDVKPFISRSFPFSQGIEAFEEAARGRPQDVKIQIEMEG 345 L++SG +D+KP I+ ++ F+ I+AFE AA+G P+DVK+QI + G Sbjct: 301 LIASGKVDLKPLITGTYDFADSIKAFERAAQGNPEDVKLQILLTG 345 >SMc01992 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Sme Sinorhizobium meliloti Length = 346 Score = 422 bits (1084), Expect = e-118, Method: Compositional matrix adjust. Identities = 206/342 (60%), Positives = 256/342 (74%) Query: 2 QALVLEATRELALRDIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMVL 61 +ALVLE +L+LRDI + +DL DV I I TVG+CGSD+HYYTHG+IGPF V PM+L Sbjct: 3 KALVLEKKGQLSLRDIPVRRDLSPTDVLIGIRTVGVCGSDVHYYTHGKIGPFVVNEPMIL 62 Query: 62 GHEASGTIIEVGSGVTQLKVGDRVCMEPGIPRLDSPATLRGLYNLDPSVRFWATPPIHGC 121 GHEA+G ++EVGS V LK GDRVCMEPGIP L S ++ G+YN+DPSVRFWATPP+HGC Sbjct: 63 GHEAAGVVLEVGSQVRHLKKGDRVCMEPGIPDLSSRSSKLGIYNVDPSVRFWATPPVHGC 122 Query: 122 LTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAMT 181 LT VVHPAAFTYRLPD+VSFAEGA+VEP +IG+QAA +A ++PGD V+GAG IG MT Sbjct: 123 LTPEVVHPAAFTYRLPDHVSFAEGAMVEPFAIGVQAALRAGIRPGDVGAVMGAGPIGMMT 182 Query: 182 ALAALAGGAARVILADVVAEKLAHFANNPAVITVDVTQQALTDVVQQVTEGWGADVVFEA 241 ALAALAGG ++V +AD+ KL + T++V QQA+++ + T GWGADVVFE Sbjct: 183 ALAALAGGCSKVYVADLAQPKLDVIGAYEGIETINVRQQAVSEALAGATGGWGADVVFEC 242 Query: 242 SGHAGVYQTLLDLVCPGGCAVLVGMPPEPVGLDVVTMQTKEVRLESVFRYANIFPRALAL 301 SG A L L PGG VLVGMP EPV D+V MQ KE+R+E+VFRYAN++ RA+ L Sbjct: 243 SGAAPAILALPSLARPGGTVVLVGMPVEPVPFDIVGMQAKELRIETVFRYANVYDRAIEL 302 Query: 302 LSSGMIDVKPFISRSFPFSQGIEAFEEAARGRPQDVKIQIEM 343 ++SG +D+KP IS + PF + I AF+ A RP DVKIQIEM Sbjct: 303 IASGKVDLKPLISATIPFDESIAAFDRAVEARPTDVKIQIEM 344 >AGl1091 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 350 Score = 409 bits (1051), Expect = e-114, Method: Compositional matrix adjust. Identities = 209/348 (60%), Positives = 261/348 (75%), Gaps = 5/348 (1%) Query: 1 MQALVLEATRELALRDIDLPQ-----DLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKV 55 M+ALVLE +L+LRD D+P +LG +DVRIR HTVGICGSD+HYYTHG+IG F V Sbjct: 1 MKALVLEEKGKLSLRDFDIPGGAGSGELGPKDVRIRTHTVGICGSDVHYYTHGKIGHFVV 60 Query: 56 EAPMVLGHEASGTIIEVGSGVTQLKVGDRVCMEPGIPRLDSPATLRGLYNLDPSVRFWAT 115 APMVLGHEASGT+IE GS VT LK+GDRVCMEPGIP S A+ G+YN+DP+VRFWAT Sbjct: 61 NAPMVLGHEASGTVIETGSDVTHLKIGDRVCMEPGIPDPTSRASKLGIYNVDPAVRFWAT 120 Query: 116 PPIHGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAG 175 PPIHGCLT V+HPAAFTY+LPDNVSFAEGA+VEP +IG+QAA +AR++PGD AVV GAG Sbjct: 121 PPIHGCLTPEVIHPAAFTYKLPDNVSFAEGAMVEPFAIGMQAALRARIQPGDIAVVTGAG 180 Query: 176 TIGAMTALAALAGGAARVILADVVAEKLAHFANNPAVITVDVTQQALTDVVQQVTEGWGA 235 IG M ALAALAGG A+VI+AD+ KL A + T+++ ++ L + V T+GWG Sbjct: 181 PIGMMVALAALAGGCAKVIVADLAQPKLDIIAAYDGIETINIRERNLAEAVSAATDGWGC 240 Query: 236 DVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPEPVGLDVVTMQTKEVRLESVFRYANIF 295 D+VFE SG A + L PGG VLVGMP +PV +D+V +Q KE+R+E+VFRYAN++ Sbjct: 241 DIVFECSGAAPAILGMAKLARPGGAIVLVGMPVDPVPVDIVGLQAKELRVETVFRYANVY 300 Query: 296 PRALALLSSGMIDVKPFISRSFPFSQGIEAFEEAARGRPQDVKIQIEM 343 RA+AL++SG +D+KP IS + PF I F+ A R DVK+QI M Sbjct: 301 DRAVALIASGKVDLKPLISATIPFEDSIAGFDRAVEARETDVKLQIVM 348 >YLR070c [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Sce Saccharomyces cerevisiae Length = 356 Score = 256 bits (653), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 143/353 (40%), Positives = 201/353 (56%), Gaps = 19/353 (5%) Query: 2 QALVLEATRELALRDIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMVL 61 +A+VLE ++ L ++ +P+ +V I+I GICGSD+HYYTHGRI + VE+PMVL Sbjct: 8 EAIVLERPGKITLTNVSIPKISDPNEVIIQIKATGICGSDIHYYTHGRIANYVVESPMVL 67 Query: 62 GHEASGTIIEVGSGVTQLKVGDRVCMEPGIPRLDSPATLRGLYNLDPSVRFWATPPIHGC 121 GHE+SG + +G V LKVGDRV +EPGIP SP G YNLDP+++F ATPP G Sbjct: 68 GHESSGIVALIGENVKTLKVGDRVALEPGIPDRFSPEMKEGRYNLDPNLKFAATPPFDGT 127 Query: 122 LTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAMT 181 LT F Y+LPD+VSF EGA++EPLS+ + A A++K G VV GAG IG + Sbjct: 128 LTKYYKTMKDFVYKLPDDVSFEEGALIEPLSVAIHANKLAKIKFGARCVVFGAGPIGLLA 187 Query: 182 ALAALAGGAARVILADVVAEKL--------AHFANN---PAVITVDVTQQALTDVVQQVT 230 A GAA V+ D++ KL H N+ P +TVD V+++ Sbjct: 188 GKVASVFGAADVVFVDLLENKLETARQFGATHIVNSGDLPHGVTVD-------SVIKKAI 240 Query: 231 EGWGADVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPEPVGLDVVTMQTKEVRLESVFR 290 GADVVFE SG + +++ GG V VGM E + + + TKE+ + FR Sbjct: 241 GKKGADVVFECSGAEPCVRAGIEVCKAGGTIVQVGMGQEEIQFPISIIPTKELTFQGCFR 300 Query: 291 YAN-IFPRALALLSSGMIDVKPFISRSFPFSQGIEAFEEAARGRPQDVKIQIE 342 Y + ++ L+SS + +KPFI+ + F +EAFEE + ++K IE Sbjct: 301 YCQGDYSDSIELVSSRKLSLKPFITHRYSFKDAVEAFEETSHHPLNNIKTIIE 353 >BS_gutB [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Bsu Bacillus subtilis Length = 353 Score = 236 bits (603), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 124/342 (36%), Positives = 200/342 (58%), Gaps = 2/342 (0%) Query: 1 MQALVLEATRELALRDIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMV 60 M+A V+ TRE+ + + +P D+ +V I++ VGICGSDLHYYT+GRIG + VE P + Sbjct: 9 MKAAVMHNTREIKIETLPVP-DINHDEVLIKVMAVGICGSDLHYYTNGRIGNYVVEKPFI 67 Query: 61 LGHEASGTIIEVGSGVTQLKVGDRVCMEPGIPRLDSPATLRGLYNLDPSVRFWATPPIHG 120 LGHE +G I VGS V Q KVGDRV +EPG+ A G YNL P V+F ATPP+ G Sbjct: 68 LGHECAGEIAAVGSSVDQFKVGDRVAVEPGVTCGRCEACKEGRYNLCPDVQFLATPPVDG 127 Query: 121 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 + F + +PD++S+ E A++EP S+G+ AA + +++PG T ++G G +G M Sbjct: 128 AFVQYIKMRQDFVFLIPDSLSYEEAALIEPFSVGIHAAARTKLQPGSTIAIMGMGPVGLM 187 Query: 181 TALAALAGGAARVILADVVAEKLAHFANNPAVITVDVTQQALTDVVQQVTEGWGADVVFE 240 AA A GA +I+ D+ +L A +++ +Q + ++ +T G DV +E Sbjct: 188 AVAAAKAFGAGTIIVTDLEPLRLEAAKKMGATHIINIREQDALEEIKTITNDRGVDVAWE 247 Query: 241 ASGHAGVYQTLLDLVCPGGCAVLVGMPPE-PVGLDVVTMQTKEVRLESVFRYANIFPRAL 299 +G+ Q+ L V GG +VG+P + + L+V + E+ + +FRYAN +P+ + Sbjct: 248 TAGNPAALQSALASVRRGGKLAIVGLPSQNEIPLNVPFIADNEIDIYGIFRYANTYPKGI 307 Query: 300 ALLSSGMIDVKPFISRSFPFSQGIEAFEEAARGRPQDVKIQI 341 L+SG++D K ++ + Q +A E A + + + +K+ + Sbjct: 308 EFLASGIVDTKHLVTDQYSLEQTQDAMERALQFKNECLKVMV 349