BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0170.p (298 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3829 [R] COG1073 Hydrolases of the alpha/beta superfamily Pae ... 170 3e-42 PA2218 [R] COG1073 Hydrolases of the alpha/beta superfamily Pae ... 94 5e-19 RSc0206 [R] COG1073 Hydrolases of the alpha/beta superfamily Rso... 92 2e-18 MA0419 [R] COG1073 Hydrolases of the alpha/beta superfamily Mac ... 89 1e-17 XF1745 [R] COG1073 Hydrolases of the alpha/beta superfamily Xfa ... 87 5e-17 >PA3829 [R] COG1073 Hydrolases of the alpha/beta superfamily Pae Pseudomonas aeruginosa Length = 307 Score = 170 bits (431), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 105/298 (35%), Positives = 159/298 (53%), Gaps = 5/298 (1%) Query: 1 MRTEIIHFQSEGDTCAGTIYRPEGDGPFPAVLLSHGFGAVRTMRNIPEVATLLAEAGILA 60 M+ + F+SEG G +Y PE PA++++HGF V+ + + A + A AG + Sbjct: 1 MQRLNVEFRSEGVALRGWLYLPEARAERPAIVMTHGFSGVKE-QYLDRYAEVFAAAGFVV 59 Query: 61 LAIDFRYLGDSGGEPRQQVRPTAQRQDLGNALSYLETREDVRTDRLGLWGTSFSGGQSLR 120 L D GDS GEPRQ++ P QR+ +A+++L + V R+G+WGTS+SGG L Sbjct: 60 LLYDHPNFGDSDGEPRQEIDPVMQRRGYRDAITWLGAQARVDASRIGIWGTSYSGGHVLE 119 Query: 121 IAAFDKRVKALVAQVPATDLYRQIKFEAPPEHRKLLESTIAAERPRHFGQLAPATMK--L 178 +AA D+RVK +VAQVP + E L ++ A+R R F L PA + Sbjct: 120 VAALDRRVKCVVAQVPTVSGHASALRRTRAEQLPALLASFDADRQRRFEGLPPALLPAVA 179 Query: 179 ADEDGKPSVFGVESWEWAQNNVREHETFHNAVTVSSLEEALQTAPADYIESIAPTPLLVI 238 A D ++ G ++ + ++ + N VT+ S E A + P +I I+PTPLL+I Sbjct: 180 ASPDEPCAMAGADAHRFFTDSAALAPNWCNRVTLRSAELARENEPGIHIGRISPTPLLMI 239 Query: 239 MAEPDKTVLTKFTKAAFERAGEPKKLITYAGLHYDVYDKPEILAMASKAARDWFVTYL 296 +A+ D T A++RA PK+L+ AG H+ Y E S+AA DWFV +L Sbjct: 240 VADRDLLTPTDLCLEAYQRALPPKRLLMLAGGHFTPYI--EHFDATSQAAADWFVRHL 295 >PA2218 [R] COG1073 Hydrolases of the alpha/beta superfamily Pae Pseudomonas aeruginosa Length = 367 Score = 93.6 bits (231), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 83/285 (29%), Positives = 130/285 (45%), Gaps = 31/285 (10%) Query: 12 GDTCAGTIYRPEGDGP--FPAVLLSHGFGAVRTMRNIPEVATLLAEAGILALAIDFRYLG 69 G T A +Y P+ G PA+++ FGAV+ ++ A +AE G + LA D Y G Sbjct: 78 GITLAADLYLPKNRGGDRLPAIVIGGPFGAVKE-QSSGLYAQTMAERGFVTLAFDPSYTG 136 Query: 70 DSGGEPRQQVRPTAQRQDLGNALSYLETREDVRTDRLGLWGTSFSGGQSLRIAAFDKRVK 129 +SGG+PR P +D A+ ++ +V +R+G+ G GG +L A DKRVK Sbjct: 137 ESGGQPRNVASPDINTEDFSAAVDFISLLPEVNRERIGVIGICGWGGMALNAVAVDKRVK 196 Query: 130 ALVAQVPATDLYRQIKFEAPPEHRKLLESTIAAERPRHFGQLAPATMKLADEDGKPSV-- 187 A+V D+ R + + +S +R R QL K A E G P+ Sbjct: 197 AVVTST-MYDMTRVMS-------KGYNDSVTLEQRTRTLEQLGQQRWKDA-ESGTPAYQP 247 Query: 188 -FGVESWEWAQNNVREHETFH-------------NAVTVSSLEEALQTAPADYIESIAPT 233 + AQ V H+ + NA T+++ + YI+ I+P Sbjct: 248 PYNELKGGEAQFLVDYHDYYMTPRGYHPRAVNSGNAWTMTTPLSFMNMPILTYIKEISPR 307 Query: 234 PLLVIMAEPDKTVLTKFTKAAFERAGEPKKLITYAGL-HYDVYDK 277 P+L+I E + F++ A+ A EPK+L+ G H D+YD+ Sbjct: 308 PILLIHGE--RAHSRYFSETAYAAAAEPKELLIVPGASHVDLYDR 350 >RSc0206 [R] COG1073 Hydrolases of the alpha/beta superfamily Rso Ralstonia solanacearum Length = 342 Score = 91.7 bits (226), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 82/286 (28%), Positives = 131/286 (45%), Gaps = 24/286 (8%) Query: 14 TCAGTIYRPEG---DGPFPAVLLSHGFGAVRTMRNIPEVATLLAEAGILALAIDFRYLGD 70 T AG ++ P G + PA+++ H GAV+ + A LAE G + L++D + G+ Sbjct: 49 TVAGNLFIPNGLNANARTPAIIVGHPMGAVKEQSS-NLYAQKLAEQGFVTLSLDLSFWGE 107 Query: 71 SGGEPRQQVRPTAQRQDLGNALSYLETREDVRTDRLGLWGTSFSGGQSLRIAAFDKRVKA 130 S G PR V P +D A+ +L TR V +R+G+ G SG + A D R+KA Sbjct: 108 SEGRPRNAVSPDIYAEDFSAAVDFLGTRPFVDRERIGVLGICGSGSFVISAAKIDPRMKA 167 Query: 131 LVAQVPATDL----YRQIKFEAPPEHRKLLESTIAAERPRHF----GQLAPATMKLADED 182 +A V D+ + P E RK + + AA+R F Q T+ D Sbjct: 168 -IATVSMYDMGAANRNALHHSLPLEQRKAIIAQAAAQRYVEFTGGETQYTSGTVHELTAD 226 Query: 183 GKP------SVFGVESWEWAQNNVREHETFHNAVTVSSLEEALQTAPADYIESIAPTPLL 236 P + E+ +T H T++S + + P + IE+I+P P+L Sbjct: 227 TDPIQREFFDFYRTPRGEYTPKGSSPKQTTHP--TLTSNVKFMNFYPFNDIETISPRPVL 284 Query: 237 VIMAEPDKTVLTKFTKAAFERAGEPKKLITYAGL-HYDVYDKPEIL 281 I D+ +F++ A+ A EPK+L G H D+YD+ ++ Sbjct: 285 FITG--DQAHSKEFSEEAYRLAAEPKELFIVRGAGHVDLYDRVSLI 328 >MA0419 [R] COG1073 Hydrolases of the alpha/beta superfamily Mac Methanosarcina acetivorans str.C2A Length = 327 Score = 89.0 bits (219), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 76/265 (28%), Positives = 121/265 (45%), Gaps = 15/265 (5%) Query: 28 FPAVLLSHGFGAVRTMRNIPEVATLLAEAGILALAIDFRYLGDSGGEPRQQVRPTAQRQD 87 +PA+++ +G V+ + A +AE G +A+A D + G+S GEPR P +D Sbjct: 53 YPAIVIGTPYGGVKE-QGAGIYAQNMAERGFVAIAFDESFNGESSGEPRHVSSPDIFVED 111 Query: 88 LGNALSYLETREDVRTDRLGLWGTSFSGGQSLRIAAFDKRVKALVAQVPATDLYRQIK-- 145 + +L TR V +R+G G SGG S+ A D R+KA VA D+ I+ Sbjct: 112 FSAGVDFLGTRLFVDRNRIGAIGICGSGGFSITTAQVDPRIKA-VATASMYDISSMIRDG 170 Query: 146 --FEAPPEHRKLLESTIAAERPRHFGQLAPAT-MKLADE--DGKPSVF---GVESWEWAQ 197 E R + +R + F P M E D P E +E+ Sbjct: 171 FGKTMTDEQRAQTLVQLGEQRWKDFENGTPEVPMGFPSEPADSIPEGLDPISSEFFEYYG 230 Query: 198 NNVREHETFHNAVTVSSLEEALQTAPADYIESIAPTPLLVIMAEPDKTVLTKFTKAAFER 257 H A T++S + +YI++I+P P+L I+ E + F++ A+ER Sbjct: 231 MERGHHPNAGAAFTITSSMSFMNFPLMNYIDTISPRPILFIIGENAHS--RYFSEDAYER 288 Query: 258 AGEPKKLITYAGL-HYDVYDKPEIL 281 A EPK+L G H D+YD+ +++ Sbjct: 289 AAEPKELYVVPGARHIDLYDRTDMI 313 >XF1745 [R] COG1073 Hydrolases of the alpha/beta superfamily Xfa Xylella fastidiosa 9a5c Length = 338 Score = 86.7 bits (213), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 76/263 (28%), Positives = 122/263 (46%), Gaps = 17/263 (6%) Query: 30 AVLLSHGFGAVRTMRNIPEVATLLAEAGILALAIDFRYLGDSGGEPRQQVRPTAQRQDLG 89 A+ + H GAV+ ++ A LAE G + L D Y G+S G+PR V P D Sbjct: 68 AIAVGHPMGAVKE-QSANLYAQKLAERGFVTLTFDLSYWGESEGQPRNAVLPDVYADDFS 126 Query: 90 NALSYLETREDVRTDRLGLWGTSFSGGQSLRIAAFDKRVKALVAQVPATDLYRQIKFEAP 149 A+ +L T+ V +R+G+ G GG + A D R+KA +A V D+ + Sbjct: 127 AAVDFLSTQPFVDRERIGVLGICAGGGFVISAAKLDPRMKA-IATVSMVDMGSAARGMVS 185 Query: 150 PEHRKLLESTIAAERPRHFG----QLAPATMKLADEDGKP------SVFGVESWEWAQNN 199 E RK + + AA+R F + T+ +D P + E+ Sbjct: 186 VEQRKQITAAAAAQRQAEFDGGKVEYTGGTVHALHDDTPPMQREFYDFYRTPRGEYTPAG 245 Query: 200 VREHETFHNAVTVSSLEEALQTAPADYIESIAPTPLLVIMAEPDKTVLTKFTKAAFERAG 259 T H T++S + L P + IE+I+P P+L I D+ + F++ A++ AG Sbjct: 246 ATRETTTHP--TLASDVKFLNFYPFNDIETISPRPMLFIAG--DQAISRGFSEQAYKLAG 301 Query: 260 EPKKLITYAGL-HYDVYDKPEIL 281 EPK+L G H D+YD+ +++ Sbjct: 302 EPKELYWVKGAGHVDLYDRVDLI 324