BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0149.p (356 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2149 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase Rso Ral... 186 1e-46 SMa1961 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase Sme Sin... 104 3e-22 mlr2444 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase Mlo Mes... 95 2e-19 Rv0671 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase Mtu Myco... 67 6e-11 MT0700 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase MtC Myco... 67 6e-11 >RSc2149 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase Rso Ralstonia solanacearum Length = 343 Score = 186 bits (471), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 110/278 (39%), Positives = 148/278 (53%), Gaps = 12/278 (4%) Query: 73 QYRLCLPKPTPSELP-LIVMLHGCQQNAADFAQGTRMNAHAAKHGFAVLYPEQSLRAHPH 131 +Y L P S P L+VMLHGC+Q+A A GTRMNA A + GF VLYPEQ RAHP Sbjct: 69 EYWLYTPPSRGSTPPALMVMLHGCKQSAEALAHGTRMNALADREGFIVLYPEQPRRAHPQ 128 Query: 132 RCWRWYAQDAQKGEGDVALVASLIQEILKTHPIDPQRIYVCGISAGAALAHLLALTHPEL 191 CW WY + G + +A L+ + L DP R+Y+ G+SAGA LA L+AL HP L Sbjct: 129 CCWHWYDPE-HAGAHEADDLARLVAQTLTETGADPSRVYLAGLSAGAGLAGLMALRHPHL 187 Query: 192 IAAVALHAAPVSGVCSNAAGAYSVMQHGSLRAAAAAKRSLSRFPRTGALPAILIGGTDDK 251 AA+ALH+ PV GV AA A ++M+ G+ A ++ +P +L+ G D+ Sbjct: 188 FAALALHSGPVLGVARTAASALNLMRRGARIDPVEALATMVDTGTYPGIPTLLLHGLRDE 247 Query: 252 IVRPVNQRQLLEQFLLLNHTQSLTEQPPRETPYGRTSKKQPRRRIVATRDYVRGRKLIIR 311 V PVNQ QL QF LNH + ++ R R +R ++ Sbjct: 248 AVSPVNQAQLATQFRALNHLCDAAD----------VRRETTRGDGYVLRSDLRDDACLLA 297 Query: 312 SVLIEGLGHAWSGGDTTLSYNAAGPDASRLVVDFFSRH 349 + GHAWSGGD +++AAGPDA+ L F +H Sbjct: 298 TCQFTSTGHAWSGGDPRYAFHAAGPDATWLWWQFARQH 335 >SMa1961 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase Sme Sinorhizobium meliloti Length = 375 Score = 104 bits (260), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 71/237 (29%), Positives = 117/237 (49%), Gaps = 15/237 (6%) Query: 88 LIVMLHGCQQNAADFAQGTRMNAHAAKHGFAVLYPEQSLRAHPHRCWRWYA-QDAQKGEG 146 L+V+LHGC QNAA + + + A GFAVLYPEQ +P+ C+ W+ D ++G+G Sbjct: 69 LVVVLHGCTQNAAGYDHASGWSKIAEDFGFAVLYPEQVPANNPNVCFNWFTPSDIRRGQG 128 Query: 147 DVALVASLIQEILKTHPIDPQRIYVCGISAGAALAHLLALTHPELIAAVALHAAPVSGVC 206 +V + +++ I+ + ID +R+Y+ G+SAG A+A+ +PE+ A+ A Sbjct: 129 EVHSIRQMVETIIVEYGIDRRRVYITGLSAGGAMANAALCAYPEIFTGGAIIAGLPFAAA 188 Query: 207 SNAAGAYSVMQ-HGSLRAAAAAKRSLSRFPRTGALPAILIG-GTDDKIVRPVNQRQLLEQ 264 + A+ M+ HG + R P G P I + GT+D+ V N + ++ Q Sbjct: 189 TTVPEAFDRMRGHGIPDVESLRSRLSGASPHAGPWPTISVWHGTNDRTVAEANAKAIIAQ 248 Query: 265 FLLLNHTQSLTEQPPRETPYGRTSKKQPRRRIVATRDYVRGRKLIIRSVLIEGLGHA 321 + ++ S P + +R +A RD R + I LIEG+GH Sbjct: 249 WSGVHGVPS--------NPSSVETVDGHKR--LAWRD--RSGRDAIELYLIEGMGHG 293 >mlr2444 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase Mlo Mesorhizobium loti Length = 371 Score = 94.7 bits (234), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 85/288 (29%), Positives = 137/288 (47%), Gaps = 25/288 (8%) Query: 71 SLQYRLCLPKPTPSELPLIVMLHGCQQNAADFAQGTRMNAHAAKHGFAVLYPEQSLRAHP 130 +L+ R P+ P L+V+LHGC Q AA + G + AA+ GFAVL+PEQ + Sbjct: 28 ALRARFYAPEDLPKGAALVVVLHGCTQTAAGYDHGAGWSRLAAEEGFAVLFPEQQRANNA 87 Query: 131 HRCWRWYA-QDAQKGEGDVALVASLIQEILKTHPIDPQRIYVCGISAGAALAHLLALTHP 189 + C+ W+ +D + G+ + +I+ ++ H +D +RI++ G+SAG A+A + T+P Sbjct: 88 NLCFNWFVPEDVTRDRGEALSIRRMIEAMVTKHGLDRERIFITGLSAGGAMAAAMLATYP 147 Query: 190 ELIAAVALHAAPVSGVCSNAAGAYSVMQ-HGSLRAAAAAKRSLSRFPRTGALPAILIG-G 247 ++ A A+ A G S A+ M+ HG A +R P G+ P I I G Sbjct: 148 DVFAGGAIIAGLAYGSASTIPEAFDRMRGHGGPTPAELHRRLAQASPHQGSWPRISIWQG 207 Query: 248 TDDKIVRPVNQRQLLEQFLLLNHTQSLTEQPPRETPYGRTSKKQPRRRIVATRDYVRGRK 307 T D V P N L+ Q+ ++ + P R R + R++ D Sbjct: 208 TADHTVAPSNADSLVAQWQSVHRVGT---GPDRAETVDRQT-----RQVWCDADG----N 255 Query: 308 LIIRSVLIEGLGHAW---SGGDTTLSYNAA------GPDASRLVVDFF 346 +I I G+GH +GG+ L NAA G ++R +V F+ Sbjct: 256 EVIEKYTIAGMGHGTPLKTGGEDGLG-NAAPFMLDVGISSTRRMVAFW 302 >Rv0671 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase Mtu Mycobacterium tuberculosis H37Rv Length = 280 Score = 67.0 bits (162), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 42/138 (30%), Positives = 67/138 (48%), Gaps = 1/138 (0%) Query: 60 SAVHHVPATTHSLQYRLCLPKPTPSELPLIVMLHGCQQNAADFAQGTRMNAHAAKHGFAV 119 ++VH + YRL P PS PL+VMLHG +A + + A F V Sbjct: 32 NSVHTLDVDGAGRSYRLYKPVGLPSSAPLVVMLHGGFGSAKQAERSYGWDELADSEKFLV 91 Query: 120 LYPEQSLRAHPHRCWRWYAQDAQKGEGDVALVASLIQEILKTHPIDPQRIYVCGISAGAA 179 YP+ RA + A++G D+ V +++ +I IDP R+YV G+S GA Sbjct: 92 AYPDGYHRAWNANGGGCCGRPAREGVDDIGFVRAVVADIANNVSIDPARVYVTGMSNGAI 151 Query: 180 LAHLLALTHPELIAAVAL 197 +++ LA + + AA+ + Sbjct: 152 MSYTLA-CNTSIFAAIGV 168 >MT0700 [Q] COG3509 Poly(3-hydroxybutyrate) depolymerase MtC Mycobacterium tuberculosis CDC1551 Length = 280 Score = 67.0 bits (162), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 42/138 (30%), Positives = 67/138 (48%), Gaps = 1/138 (0%) Query: 60 SAVHHVPATTHSLQYRLCLPKPTPSELPLIVMLHGCQQNAADFAQGTRMNAHAAKHGFAV 119 ++VH + YRL P PS PL+VMLHG +A + + A F V Sbjct: 32 NSVHTLDVDGAGRSYRLYKPVGLPSSAPLVVMLHGGFGSAKQAERSYGWDELADSEKFLV 91 Query: 120 LYPEQSLRAHPHRCWRWYAQDAQKGEGDVALVASLIQEILKTHPIDPQRIYVCGISAGAA 179 YP+ RA + A++G D+ V +++ +I IDP R+YV G+S GA Sbjct: 92 AYPDGYHRAWNANGGGCCGRPAREGVDDIGFVRAVVADIANNVSIDPARVYVTGMSNGAI 151 Query: 180 LAHLLALTHPELIAAVAL 197 +++ LA + + AA+ + Sbjct: 152 MSYTLA-CNTSIFAAIGV 168