BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0126.p (416 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2521 [M] COG0845 Membrane-fusion protein Pae Pseudomonas aerug... 373 e-103 RSp0492 [M] COG0845 Membrane-fusion protein Rso Ralstonia solana... 347 3e-95 CC2722 [M] COG0845 Membrane-fusion protein Ccr Caulobacter vibri... 124 5e-28 aq_468 [M] COG0845 Membrane-fusion protein Aae Aquifex aeolicus 116 1e-25 CC2389 [M] COG0845 Membrane-fusion protein Ccr Caulobacter vibri... 115 2e-25 >PA2521 [M] COG0845 Membrane-fusion protein Pae Pseudomonas aeruginosa Length = 484 Score = 373 bits (957), Expect = e-103, Method: Compositional matrix adjust. Identities = 192/345 (55%), Positives = 250/345 (72%), Gaps = 1/345 (0%) Query: 72 KEEGKIALSKEQMAAAGIRVDTAGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVS 131 +EEGK+ L EQ+ AAGI +DTA + + V LPGEIRFNEDRTAH+VP++ GIV+SV Sbjct: 141 REEGKLELDAEQIEAAGITLDTARTISLAQVVSLPGEIRFNEDRTAHIVPRLPGIVDSVP 200 Query: 132 ANLGQKVKKGQVLAVISSSAVSEQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYL 191 ANLGQ VK+G++LAVISS +S+QRS+ A++RLSLA+S Y+RE+ LW++ ISAEQ++L Sbjct: 201 ANLGQAVKQGELLAVISSPQLSDQRSEFAAAQRRLSLAQSTYKREQQLWKEGISAEQEFL 260 Query: 192 QAQAALREAEVAVRNAQQKLRAIGATGTSSGNLNRYEIRAPFDGIVVEKHLGLGEAVRED 251 A+ L+EAE+A+ NA+ K+ A+G + G NRYE+RAPF G++VEKHL GE V Sbjct: 261 LARQGLQEAEIALNNARAKIAALGGNPSLQGG-NRYELRAPFAGVLVEKHLTQGEPVDGT 319 Query: 252 ANAFLISDLSSVWAEIIVSPKDIQVVRVGEKVVVRATAFDASAEGKISYVGSLIGEQSRT 311 AN F +SDLSSVWA V + + VRVG KV V A A D+ EG +SY+G L+GEQ+R Sbjct: 320 ANVFTLSDLSSVWATFNVPAQLLGQVRVGSKVKVLAQALDSEVEGTVSYIGDLLGEQTRA 379 Query: 312 AKAHVVLPNTGDLWRPGLFVNVEVIAGETQVPVAVAADAIQTVEGENVVFVRDADGFVAK 371 A A V L N WRPGLFV+V+V + + VA A+Q V+GE+VVFVR ADGFV + Sbjct: 380 ATARVTLSNPESTWRPGLFVSVQVAEATRKEVLTVADGAVQNVDGEDVVFVRVADGFVVQ 439 Query: 372 AVSLGRSDGKSVEITSGLDAGTGYAATGSFAIKAELGKGSAEHSH 416 V LG SDG+ VE+ GL AG+ AA+GSF +K+ELGKGSAEH H Sbjct: 440 PVKLGISDGQRVEVLEGLRAGSQVAASGSFILKSELGKGSAEHGH 484 >RSp0492 [M] COG0845 Membrane-fusion protein Rso Ralstonia solanacearum Length = 404 Score = 347 bits (890), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 187/340 (55%), Positives = 242/340 (71%), Gaps = 2/340 (0%) Query: 77 IALSKEQMAAAGIRVDTAGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQ 136 IA++ E +A A I V+TAG Q+R+ V LPGEIR N+DRTAHVVP+VAG+ E V+ LGQ Sbjct: 67 IAMTAEAIANAAIGVETAGAAQLRTEVVLPGEIRLNDDRTAHVVPRVAGVAEQVAVELGQ 126 Query: 137 KVKKGQVLAVISSSAVSEQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYLQAQAA 196 V +GQ+LAV+SS A+S+QRS+L A+QRL+LA+ A+ REK LWEQ I+A QDY QA+ A Sbjct: 127 PVTRGQLLAVLSSPALSDQRSELQAAQQRLALAQEAHAREKRLWEQGIAAAQDYQQARTA 186 Query: 197 LREAEVAVRNAQQKLRAIGATGTSSGNLNRYEIRAPFDGIVVEKHLGLGEAVREDANAFL 256 L+EA +A NA+QKL AIGA + LNR+E+RAPFDG+VV +HL GEAV+ +A F Sbjct: 187 LQEARIAADNARQKLAAIGAAPAGTA-LNRFELRAPFDGVVVARHLSQGEAVQAEAAVFT 245 Query: 257 ISDLSSVWAEIIVSPKDIQVVRVGEKVVVRATAFDASAEGKISYVGSLIGEQSRTAKAHV 316 I+DL +VWA+ V+ KD++ V VRATA + +GK+SYVG+L+GEQ+R+A A V Sbjct: 246 IADLRTVWADFAVTAKDLEAVTTHATATVRATATGTAVQGKVSYVGALLGEQTRSAPARV 305 Query: 317 VLPNTGDLWRPGLFVNVEVIAGETQVPVAVAADAIQTVEGENVVFVRDADGFVAKAVSLG 376 L N WRPG+FV+V V +G VPVAV ADA+QTV+ + VFV GF + V G Sbjct: 306 TLDNPKRAWRPGMFVSVSVESGRVPVPVAVRADAVQTVDEKPSVFVPVPAGFEVRPVKTG 365 Query: 377 RSDGKSVEITSGLDAGTGYAATGSFAIKAELGKGSAEHSH 416 RSDG EI GL AGT YAA S+ +KAE GK SA+H H Sbjct: 366 RSDGTWTEILDGLPAGTRYAAANSYVLKAEAGK-SADHGH 404 >CC2722 [M] COG0845 Membrane-fusion protein Ccr Caulobacter vibrioides Length = 411 Score = 124 bits (310), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 119/375 (31%), Positives = 182/375 (48%), Gaps = 20/375 (5%) Query: 49 EGGHKDKTA-HDDDKDDEHKEGEHKEEGKIALSKEQMAAAGIRVDTAGPLQIRSTVQLPG 107 EGG D A H + K G+ +EG I +S E++AA+GIRV+T + + + Sbjct: 50 EGGEADHVAGHIESKAGV---GQTDKEGHIDMSAERLAASGIRVETLMAGGLSAGIVAQA 106 Query: 108 EIRFNEDRTAHVVPQVAGIVESVSANLGQKVKKGQVLAVISSSAVSEQRSDLLNAEQRLS 167 + + D A + + G V ++ LG V G+ LA+I S + S+ A + + Sbjct: 107 SVVGSPDGAATLAARADGAVVRINKRLGDAVAAGEPLALIESRDAAAISSEYAAAAAKAT 166 Query: 168 LARSAYQREKHLWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRAIGATGTSSGNLNRY 227 AR + RE+ L++ KI+A QD A A + AE +R A + A A G S+ RY Sbjct: 167 AARQTFVRERRLFDAKITARQDLEAALAEIAIAEAELRRA---INAGAAAGVSADG--RY 221 Query: 228 -EIRAPFDGIVVEKHLGLGEAVREDANAFLISDLSSVWAEIIVSPKDIQVVRVGEKVVVR 286 + +P G + L LG V F ++D + + + D + G++ ++ Sbjct: 222 VTVSSPIAGKITAAPLVLGSYVAAGTELFRVADPRKIEVQAWLPASDAARIAAGDQALIE 281 Query: 287 ATAFDASAEGKISYVGSLIGEQSRTAKAHVVLPNTGDLWRPGLFVNVEVI-----AGETQ 341 + + + + + +SR A A + L PG V V +I AGE Sbjct: 282 TS--QGALPAVVRSITPGVDVESRAATAILTLRQGHKGLVPGQAVRVRIIPRGAAAGER- 338 Query: 342 VPVAVAADAIQTVEGENVVFVRDADGFVAKAVSLGRSDGKSVEITSGLDAGTGYAATGSF 401 V+V +A+Q+V+G + VFVR DGF A+ V +GR G VEI SGL AG A G+F Sbjct: 339 --VSVPEEAVQSVKGADSVFVRGKDGFTARPVKIGRRGGDRVEILSGLTAGEQIAGQGAF 396 Query: 402 AIKAELGKGSAEHSH 416 +KAELGKG AEH H Sbjct: 397 LLKAELGKGEAEHGH 411 >aq_468 [M] COG0845 Membrane-fusion protein Aae Aquifex aeolicus Length = 359 Score = 116 bits (290), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 91/339 (26%), Positives = 158/339 (46%), Gaps = 9/339 (2%) Query: 79 LSKEQMAAAGIRVDTAGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQKV 138 L+ +Q+ GI++ + V++P E+ N V V GIV + G V Sbjct: 29 LTDKQIKLLGIKLYEVKREPVGKIVKMPAEVEENPLLNYVVYSPVEGIVRKLYKKEGDLV 88 Query: 139 KKGQVLAVISSSAVSEQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYLQAQAALR 198 KKG+ LA + S ++ ++ A+ R+ AR Y+R K L+EQK+ + A Sbjct: 89 KKGEPLAEVYSPELANLIGEVRTAKVRMESARKLYERYKKLYEQKVIKYTLFYNAMVEYE 148 Query: 199 EAEVAVRNAQQKLRAIGATGTSSGNLNRYEIRAPFDGIVVEKHLGLGEAVREDANAFLIS 258 A+ KL++ G NL +R+P G VV++++ LGE+V D F I Sbjct: 149 RAKGEYEALLSKLKSFGE--VKGYNLV---LRSPGKGYVVKQNVVLGESVGLDNFLFKIH 203 Query: 259 DLSSVWAEIIVSPKDIQVVRVGEKVVVRATAFDASAEGKISYVGSLIGEQSRTAKAHVVL 318 VW K + ++ G K A + + ++ Y+G + ++R K V Sbjct: 204 SHEVVWVYGWADEKSAKDIKEGMKGT--ALSHEDRLPCRVDYIGHEVDRKTRRVKVRCVA 261 Query: 319 PNTGDLWRPGLFVNVEVIAGETQVPVAVAADAIQTVEGENVVFVRDADGFVAKAVSLGRS 378 N + +PG+FV + ++ + + + + +A+Q +EG+ VVFVR +GF A+ V++ + Sbjct: 262 QNKNHVLKPGMFVEL-LVKTQGEPAILIPKNAVQEIEGKKVVFVRTEEGFEARPVTILKE 320 Query: 379 DGKSVEITSGLDAGTGYAATGSFAIKAEL-GKGSAEHSH 416 + GL G A TG+ +K +L G H+H Sbjct: 321 LNGYYVVLEGLKEGERIAVTGTVFLKTKLVGVEEGGHAH 359 >CC2389 [M] COG0845 Membrane-fusion protein Ccr Caulobacter vibrioides Length = 420 Score = 115 bits (288), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 72/198 (36%), Positives = 105/198 (53%), Gaps = 2/198 (1%) Query: 220 SSGNLNRYEIRAPFDGIVVEKHLGLGEAVREDANAFLISDLSSVWAEIIVSPKDIQVVRV 279 SS +L Y I AP G++VEK+L +G+ V D F+I+D + + AE V P+D + VRV Sbjct: 224 SSESLQTYAIPAPISGVIVEKNLNVGD-VTGDRAIFVIADPTKLHAEFFVYPRDAEKVRV 282 Query: 280 GEKVVVRATAFDASAEGKISYVGSLIGEQSRTAKAHVVLPNTGD-LWRPGLFVNVEVIAG 338 G+ V VR+ + D ++ V S+T AHV LP D +RPG+ V G Sbjct: 283 GQPVEVRSLSGDTKLRAEVEAVLPTADLTSQTLLAHVHLPGGADSAFRPGMGVEGSFQVG 342 Query: 339 ETQVPVAVAADAIQTVEGENVVFVRDADGFVAKAVSLGRSDGKSVEITSGLDAGTGYAAT 398 P+AV AIQ VVFV+ + + + + LGR + E+ SGL G+ Y Sbjct: 343 AATAPLAVRTKAIQRFRDFQVVFVKVGETYEVRMLELGRQTPEWTEVLSGLKPGSEYVTD 402 Query: 399 GSFAIKAELGKGSAEHSH 416 G+F I+A++ K A H H Sbjct: 403 GAFLIRADIEKSGASHDH 420