BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0114.p (315 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the T... 197 2e-50 AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the T... 118 2e-26 RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the T... 95 2e-19 Cgl0451 Non COG 72 2e-12 >YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Ype Yersinia pestis Length = 289 Score = 197 bits (502), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 110/268 (41%), Positives = 146/268 (54%), Gaps = 2/268 (0%) Query: 50 LPADACDCHMHIYDPRFAWAPGAKLLHAPATVP--MYRRLQQRLGTTRNVVVTPSAYGVD 107 LP DACD H H+ PR + A+ + P P Y + G +R V+V S YG D Sbjct: 22 LPEDACDSHAHVISPRAIYPMVAERSYTPPPAPEEKYLSMLAATGMSRGVLVQISVYGSD 81 Query: 108 NSCTLDALAQLGATARGVAVVNDQVSDAELQRLHQAGVRGLRFNLALGSVTTVEMMEPLA 167 N L L + RGVAVV + V+D ELQ + AGVRGLR N+ G + ME LA Sbjct: 82 NRYMLQVLKRHPEYLRGVAVVTEDVTDQELQDMALAGVRGLRINVLFGGGIGFDAMENLA 141 Query: 168 RRVAALGWHLQVNMPNDVLLANRALLSALPVPLVFDHFARIPLQAGPGHPVFDFVTGLMR 227 ++A LGWH+Q M L A + LP P V DH +P+ G HP + + ++R Sbjct: 142 SKIAPLGWHMQFLMDVRQLPALIPRMKKLPCPCVIDHMGHMPVSLGLNHPGYQTLLHMVR 201 Query: 228 EQRASVKLSGAYLYSKRGAPDYEDVAPLARTLVQTAPSQVVWGSDWPHPTEQHKPDDARL 287 E VKLSGAY S+ Y DV P A+ L++TAP ++VWGSDWPH + PD L Sbjct: 202 EYGWWVKLSGAYRISESWQDGYLDVVPFAQKLIETAPDRMVWGSDWPHVSVSRMPDTGGL 261 Query: 288 LDVMTTWLGSDAMLRTILVDNPARLYQF 315 +++ W+ + + ILVDNPARLY F Sbjct: 262 RNLLAAWVPDEQVRNRILVDNPARLYDF 289 >AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 292 Score = 118 bits (295), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 79/271 (29%), Positives = 125/271 (46%), Gaps = 6/271 (2%) Query: 50 LPADACDCHMHIYDPRFAWAPGAKLLHAPATVPM--YRRLQQRLGTTRNVVVTPSAYGVD 107 P A D MH+Y P + PG L A YRRL Q LG R ++ +A+ D Sbjct: 17 FPRGAVDTQMHMYLPGYPALPGGPGLPPGALPGPEDYRRLMQWLGIDRVIITQGNAHQRD 76 Query: 108 NSCTLDALAQLGATARGVAVVNDQVSDAELQRLHQAGVRGLRFNLALGSVTTVEMMEPLA 167 N TL +A++G A V +++ ++ ++++L AG G R G + ++ + Sbjct: 77 NGNTLACVAEMGEAAHAVVIIDATTTEKDMEKLTAAGTVGARIMDLPGGAVNLSELDAVD 136 Query: 168 RRVAALGWHLQVNMPNDVLLANRALLSALPVPLVFDHFARIPLQAGPGHPVFDFVTGLMR 227 R A W + V + LL + L + VFDH + P + L+ Sbjct: 137 ERAHAADWMVAVQFDGNGLLDHLPRLQKIRSRWVFDHHGKFFKGIRTDGPEMAALLKLID 196 Query: 228 EQRASVKLSGAYLYSKRGAPDYEDVAPLARTLVQTAPSQVVWGSDWPHPTEQHK---PDD 284 K +G Y S++ P Y DVA +R + AP ++VWG++WPH + + PDD Sbjct: 197 RGNLWFKFAGVYESSRKSWP-YADVAAFSRVIAAHAPERIVWGTNWPHNSVRETAAYPDD 255 Query: 285 ARLLDVMTTWLGSDAMLRTILVDNPARLYQF 315 ARL ++ WL +A LV+NP L++ Sbjct: 256 ARLAELTLGWLPDEAARHRALVENPEALFKL 286 >RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Rso Ralstonia solanacearum Length = 302 Score = 95.1 bits (235), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 100/320 (31%), Positives = 140/320 (43%), Gaps = 27/320 (8%) Query: 4 LPSRRAFLRHSTRLALGLGAIAQLP-LARAQTEPYAHSAGTQHAASRLPADACDCHMHIY 62 +P+RRAF +T L LGA A + R P A G D H H++ Sbjct: 1 MPTRRAF---NTGLLAMLGATALAGCVTRGMPAPTARITG------------IDTHAHVF 45 Query: 63 DPRFAWAPGAKLLHAP---ATVPMYRRLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLG 119 A GA+ +AP AT+P Y G + V++ PS G DNS L AL Q Sbjct: 46 KRGLPLA-GARR-YAPSYDATLPAYLAQLDAHGLSHGVLIQPSFLGGDNSYLLAALRQAP 103 Query: 120 ATARGVAVVNDQVSDAELQRLHQAGVRGLRFNL--ALGSVTTVEMMEPLARRVAALGWHL 177 RGVAV++ + L +L+ G+ G+R NL A + R+ ALGWH+ Sbjct: 104 QRLRGVAVIDPAAPEPFLAQLNAEGIVGIRLNLIGAPDPQLRAPAWQAALHRLHALGWHV 163 Query: 178 QVNMPNDVLLANRALLSALPVPLVFDHFARIPLQAGPGHPVFDFVTGLMREQRASVKLSG 237 +++ L A L V +V DHF R P F + R +R VK+SG Sbjct: 164 ELHTQAHRLPALLPPLLEAQVDVVVDHFGRPDPALSVEDPGFAALLAAGRTRRVWVKVSG 223 Query: 238 AYLYSKRGAPDYEDVAPLARTLVQTAPSQVVWGSDWPHPTEQHKP--DDARLLDVMTTWL 295 AY G + +A + R ++VWGSDWPH + + D AR T L Sbjct: 224 AYRNGDAGRGEAIALAAMPRLKDALGTDRLVWGSDWPHTQYESRIGYDSARAF--ADTLL 281 Query: 296 GSDAMLRTILVDNPARLYQF 315 +LV+ PARL++F Sbjct: 282 PDAGDRHQVLVETPARLFRF 301 >Cgl0451 Non COG Length = 250 Score = 71.6 bits (174), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 70/263 (26%), Positives = 109/263 (41%), Gaps = 19/263 (7%) Query: 52 ADACDCHMHIYDPRFAWAPGAKLLHAPATVPMYRRLQQRLGTTRNVVVTPSAYGVDNSCT 111 A D H HI DP+ L P TV Y + L +V+ S D Sbjct: 2 AKLFDSHFHIIDPQHPLIENNGYLPEPFTVEDYTARVEGLEVAGGAIVSGSFQAFDQGYL 61 Query: 112 LDALAQLGATARGVAVVNDQVSDAELQRLHQAGVRGLRFNLALGSVTTVEMMEPLARRVA 171 DALA LG GV + SD E+ L +AGV+ +R NL G ++ +E LARRV Sbjct: 62 KDALAVLGPGYVGVTQIPADTSDQEILDLDKAGVKAVRLNLKRGGSAGLDDLETLARRVH 121 Query: 172 AL-GWHLQVNMPNDVLLANRALLSALPVPLVFDHFARIPLQAGPGHPVFDFVTGLMREQR 230 L GWH ++ + L + L++LP + DH H D + L+R Sbjct: 122 DLAGWHTELYVDARELDELESTLASLPA-VSIDHLGL--------HR--DGLPALLRLVE 170 Query: 231 ASVKLSGAYLYSKRGAPDYEDVAPLARTLVQTAPSQVVWGSDWPHPTEQHKPDDARLLDV 290 +K+ P + + ++ P+ ++ G+D P T +P + LD+ Sbjct: 171 NGIKVKATGFGRVELDP-----TEVIQAIMAVDPTALMIGTDLP-STRTKRPFEDADLDL 224 Query: 291 MTTWLGSDAMLRTILVDNPARLY 313 + +G D + + +N A Y Sbjct: 225 IAETVGED-HVDNVFWNNAAAFY 246