BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0109.p (288 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2218 [R] COG1073 Hydrolases of the alpha/beta superfamily Pae ... 487 e-137 XF1750 [R] COG1073 Hydrolases of the alpha/beta superfamily Xfa ... 467 e-131 ECs0310 [R] COG1073 Hydrolases of the alpha/beta superfamily Ecs... 359 4e-99 Z0347 [R] COG1073 Hydrolases of the alpha/beta superfamily EcZ E... 358 7e-99 MA0419 [R] COG1073 Hydrolases of the alpha/beta superfamily Mac ... 314 1e-85 >PA2218 [R] COG1073 Hydrolases of the alpha/beta superfamily Pae Pseudomonas aeruginosa Length = 367 Score = 487 bits (1253), Expect = e-137, Method: Compositional matrix adjust. Identities = 229/288 (79%), Positives = 254/288 (88%), Gaps = 1/288 (0%) Query: 1 VLAGDLYEPQNISG-RIAAIVVSGPFGAVKEQSSGLYAQTMAERGFVTLAFDPSFTGESG 59 LA DLY P+N G R+ AIV+ GPFGAVKEQSSGLYAQTMAERGFVTLAFDPS+TGESG Sbjct: 80 TLAADLYLPKNRGGDRLPAIVIGGPFGAVKEQSSGLYAQTMAERGFVTLAFDPSYTGESG 139 Query: 60 GEPRNVASPDINTEDFSAAVDFIGLLPHVDRQRIGMIGVCGWGGMALNAAAVDKRVKAVV 119 G+PRNVASPDINTEDFSAAVDFI LLP V+R+RIG+IG+CGWGGMALNA AVDKRVKAVV Sbjct: 140 GQPRNVASPDINTEDFSAAVDFISLLPEVNRERIGVIGICGWGGMALNAVAVDKRVKAVV 199 Query: 120 ASTMYDMTRVLSRGYNDSVTLAQRTELLEQLGQQRWKDAERGTPEHGPVMNQLKGGEPPF 179 STMYDMTRV+S+GYNDSVTL QRT LEQLGQQRWKDAE GTP + P N+LKGGE F Sbjct: 200 TSTMYDMTRVMSKGYNDSVTLEQRTRTLEQLGQQRWKDAESGTPAYQPPYNELKGGEAQF 259 Query: 180 LVQYHDYYRTPRGFHPRSVNSNASWTRTMPLSFMNMPILSYIKEISPRPLLLIHGEKAHS 239 LV YHDYY TPRG+HPR+VNS +WT T PLSFMNMPIL+YIKEISPRP+LLIHGE+AHS Sbjct: 260 LVDYHDYYMTPRGYHPRAVNSGNAWTMTTPLSFMNMPILTYIKEISPRPILLIHGERAHS 319 Query: 240 RYFSETAFAAAAEPKELVIIPGAVHVDLYDRVDLIPFDKLTSFFRTHL 287 RYFSETA+AAAAEPKEL+I+PGA HVDLYDR+D IPFD++ FF HL Sbjct: 320 RYFSETAYAAAAEPKELLIVPGASHVDLYDRLDRIPFDRIAGFFDEHL 367 >XF1750 [R] COG1073 Hydrolases of the alpha/beta superfamily Xfa Xylella fastidiosa 9a5c Length = 378 Score = 467 bits (1201), Expect = e-131, Method: Compositional matrix adjust. Identities = 227/288 (78%), Positives = 262/288 (90%) Query: 1 VLAGDLYEPQNISGRIAAIVVSGPFGAVKEQSSGLYAQTMAERGFVTLAFDPSFTGESGG 60 LAGDLY+P+N SG++AA+ VSGPFGAVKEQSSGLYAQTMAERGF+TLAFDPS+TGES G Sbjct: 88 TLAGDLYQPKNASGKLAALAVSGPFGAVKEQSSGLYAQTMAERGFITLAFDPSYTGESSG 147 Query: 61 EPRNVASPDINTEDFSAAVDFIGLLPHVDRQRIGMIGVCGWGGMALNAAAVDKRVKAVVA 120 EPRNVASPDI+ EDFSAAVD +GLLP+VDR+RIG+IG+CGWGGMAL+AAAVDKR+KAVVA Sbjct: 148 EPRNVASPDISVEDFSAAVDCLGLLPNVDRERIGIIGICGWGGMALSAAAVDKRIKAVVA 207 Query: 121 STMYDMTRVLSRGYNDSVTLAQRTELLEQLGQQRWKDAERGTPEHGPVMNQLKGGEPPFL 180 STMYDMTRV+S+GYNDSVTL QRT+ LEQL QQRWKDAE G P + P N+LKGGE FL Sbjct: 208 STMYDMTRVMSKGYNDSVTLEQRTQTLEQLSQQRWKDAEAGKPAYQPPYNELKGGEAQFL 267 Query: 181 VQYHDYYRTPRGFHPRSVNSNASWTRTMPLSFMNMPILSYIKEISPRPLLLIHGEKAHSR 240 V+YHDYY T RG+HPR+VNS +WT+T P FMNMPIL+YI+EISPRP+LL+HGEKAHSR Sbjct: 268 VEYHDYYMTARGYHPRAVNSGNAWTQTTPFPFMNMPILTYIQEISPRPMLLVHGEKAHSR 327 Query: 241 YFSETAFAAAAEPKELVIIPGAVHVDLYDRVDLIPFDKLTSFFRTHLA 288 YFSETA+AAAAEPKELVI+PGAVHVDLYD++D IPFDKLT+FF+ HLA Sbjct: 328 YFSETAYAAAAEPKELVIVPGAVHVDLYDKMDKIPFDKLTAFFQKHLA 375 >ECs0310 [R] COG1073 Hydrolases of the alpha/beta superfamily Ecs Escherichia coli O157:H7 Length = 378 Score = 359 bits (921), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 187/310 (60%), Positives = 220/310 (70%), Gaps = 24/310 (7%) Query: 1 VLAGDLYEPQNISGR-IAAIVVSGPFGAVKEQSSGLYAQTMAERGFVTLAFDPSFTGESG 59 L GDLY P++ R +AAI VSGPFGAVKEQSSGLYAQT+AERGFVTLAFDPS+TGESG Sbjct: 69 TLVGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG 128 Query: 60 GEPRNVASPDINTEDFSAAVDFIGLLPHVDRQRIGMIGVCGWGGMALNAAAVDKRVKAVV 119 G PRNVASPDINTEDFSAAVDF+GL VDR RIG++G+CGWGGMALN AA+D RVKAV Sbjct: 129 GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKAVA 188 Query: 120 ASTMYDMTRVLSRGYNDS---VTLAQRTELLEQLGQQRWKDAERGTPEHG------PVMN 170 S MYDM+R + G D + A R +L+ L +QRWKDA+ GT HG Sbjct: 189 TSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINVDSNG 248 Query: 171 QLKGGE-----------PPFLVQYHDYYRTPRGFHPRSVNSNASWTRTMPLSFMNMPILS 219 ++ GE P L ++ DYYR PRGFH RSVNS +WT TMPLSFMNMP+LS Sbjct: 249 KVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFMNMPLLS 308 Query: 220 YIKEISPRPLLLIHGEKAHSRYFSETAF-AAAAEPKELVIIPGAVHVDLYDRV-DLIPFD 277 Y EI+ P L++ GEKAHSRYF+E A+ A ++ KELV++PGA HVDLYD V IPF Sbjct: 309 YASEITI-PTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNVAGKIPFA 367 Query: 278 KLTSFFRTHL 287 K FF+T L Sbjct: 368 KFEQFFQTKL 377 >Z0347 [R] COG1073 Hydrolases of the alpha/beta superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 352 Score = 358 bits (920), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 187/310 (60%), Positives = 220/310 (70%), Gaps = 24/310 (7%) Query: 1 VLAGDLYEPQNISGR-IAAIVVSGPFGAVKEQSSGLYAQTMAERGFVTLAFDPSFTGESG 59 L GDLY P++ R +AAI VSGPFGAVKEQSSGLYAQT+AERGFVTLAFDPS+TGESG Sbjct: 43 TLVGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG 102 Query: 60 GEPRNVASPDINTEDFSAAVDFIGLLPHVDRQRIGMIGVCGWGGMALNAAAVDKRVKAVV 119 G PRNVASPDINTEDFSAAVDF+GL VDR RIG++G+CGWGGMALN AA+D RVKAV Sbjct: 103 GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKAVA 162 Query: 120 ASTMYDMTRVLSRGYNDS---VTLAQRTELLEQLGQQRWKDAERGTPEHG------PVMN 170 S MYDM+R + G D + A R +L+ L +QRWKDA+ GT HG Sbjct: 163 TSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINVDSNG 222 Query: 171 QLKGGE-----------PPFLVQYHDYYRTPRGFHPRSVNSNASWTRTMPLSFMNMPILS 219 ++ GE P L ++ DYYR PRGFH RSVNS +WT TMPLSFMNMP+LS Sbjct: 223 KVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFMNMPLLS 282 Query: 220 YIKEISPRPLLLIHGEKAHSRYFSETAF-AAAAEPKELVIIPGAVHVDLYDRV-DLIPFD 277 Y EI+ P L++ GEKAHSRYF+E A+ A ++ KELV++PGA HVDLYD V IPF Sbjct: 283 YASEITI-PTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNVAGKIPFA 341 Query: 278 KLTSFFRTHL 287 K FF+T L Sbjct: 342 KFEQFFQTKL 351 >MA0419 [R] COG1073 Hydrolases of the alpha/beta superfamily Mac Methanosarcina acetivorans str.C2A Length = 327 Score = 314 bits (805), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 154/294 (52%), Positives = 201/294 (68%), Gaps = 10/294 (3%) Query: 1 VLAGDLYEPQNI--SGRIAAIVVSGPFGAVKEQSSGLYAQTMAERGFVTLAFDPSFTGES 58 + D+Y ++I S + AIV+ P+G VKEQ +G+YAQ MAERGFV +AFD SF GES Sbjct: 36 TVVADMYLSKDIDMSKKYPAIVIGTPYGGVKEQGAGIYAQNMAERGFVAIAFDESFNGES 95 Query: 59 GGEPRNVASPDINTEDFSAAVDFIGLLPHVDRQRIGMIGVCGWGGMALNAAAVDKRVKAV 118 GEPR+V+SPDI EDFSA VDF+G VDR RIG IG+CG GG ++ A VD R+KAV Sbjct: 96 SGEPRHVSSPDIFVEDFSAGVDFLGTRLFVDRNRIGAIGICGSGGFSITTAQVDPRIKAV 155 Query: 119 VASTMYDMTRVLSRGYNDSVTLAQRTELLEQLGQQRWKDAERGTPE-----HGPVMNQLK 173 ++MYD++ ++ G+ ++T QR + L QLG+QRWKD E GTPE + + Sbjct: 156 ATASMYDISSMIRDGFGKTMTDEQRAQTLVQLGEQRWKDFENGTPEVPMGFPSEPADSIP 215 Query: 174 GGEPPFLVQYHDYYRTPRGFHPRSVNSNASWTRTMPLSFMNMPILSYIKEISPRPLLLIH 233 G P ++ +YY RG HP N+ A++T T +SFMN P+++YI ISPRP+L I Sbjct: 216 EGLDPISSEFFEYYGMERGHHP---NAGAAFTITSSMSFMNFPLMNYIDTISPRPILFII 272 Query: 234 GEKAHSRYFSETAFAAAAEPKELVIIPGAVHVDLYDRVDLIPFDKLTSFFRTHL 287 GE AHSRYFSE A+ AAEPKEL ++PGA H+DLYDR D+IPFDKL SFF +L Sbjct: 273 GENAHSRYFSEDAYERAAEPKELYVVPGARHIDLYDRTDMIPFDKLESFFSENL 326