BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0040.p (308 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the T... 139 6e-33 mll5767 Non COG 87 6e-17 SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the ... 75 1e-13 BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the ... 73 7e-13 AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the T... 72 2e-12 >AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 287 Score = 139 bits (351), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 96/287 (33%), Positives = 139/287 (48%), Gaps = 19/287 (6%) Query: 22 MRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHASITVQARPGHEET 81 M IDAHQHFW + W + + +D LP PLL A G+ ++ VQ +T Sbjct: 15 MLIDAHQHFWLMKDRAGQWPPPSLAAIYRDFLPTDLSPLLTAAGVSGTVLVQTMETAADT 74 Query: 82 GFLLEMARRDARIAGVIAWDDIASPQL-AERMEQWGRPKMLGLRHPMQDEDDAAQALVQP 140 F+LE+A I GV+ W D+ S ++ AE + P G+R +Q +D A + Sbjct: 75 DFMLELAENTDFIKGVVGWVDMKSAEVTAEIARRAKHPAFKGVRPMLQGMEDVAW-IDDA 133 Query: 141 QFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPALKEFRKGRAAY 200 N V L + V + L +LP L + H + VV++H KP + Y Sbjct: 134 ALNAAVEALVKHGLVFDALVLPPNLPHLLSFASKHPELPVVIDHGAKPLI-----ATGHY 188 Query: 201 ERWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTALELFGPQRLMFG 260 WR+ + LAA P+V CK+SGLLTE R R + +T LELFG R++FG Sbjct: 189 RDWRRNMAALAARPNVHCKLSGLLTERGEQRPEAVRPY------AETILELFGGDRVIFG 242 Query: 261 SDWPVCLLAASYARVVAVVREWAARRLSPA-DQDALWGESSARIYGL 306 SDWPV LA + + R+ + PA D A++G ++ R Y L Sbjct: 243 SDWPVLRLAGDFQGWLDFCRD-----IVPAKDHAAVFGGNAIRFYSL 284 >mll5767 Non COG Length = 297 Score = 86.7 bits (213), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 76/303 (25%), Positives = 128/303 (42%), Gaps = 32/303 (10%) Query: 24 IDAHQHFWRYRSEDYPWIGTGMDLLAQD-----RLPYQFQPLLD-------AQGLHASIT 71 ID H H W YPW+ G+ A R Y+ L+ + +H + Sbjct: 7 IDPHHHLWDLEVNYYPWLTDGVKPSAFGDYEAIRTNYRLDDYLEDARNQNLVKSVHLDVG 66 Query: 72 VQARPGHEETGFLLEMARRDARIAGVIAWDDIASPQLAERMEQ-WGRPKMLGLRHPMQDE 130 ET +L E+A + G++ + D++ P + E +++ P G+R M Sbjct: 67 FDPSDPVGETRWLQEIANKRGFPHGIVGYADLSKPDVGELLDRHMEYPNFRGIRQSMNYH 126 Query: 131 DDAAQALV-------QPQFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLN 183 D A+ P++ G R L +R ++ + + + QL ++LN Sbjct: 127 ADPAKTYQARPEVSRTPEWRRGFRELAKRRLSFDLQLYYPQMEEFLQLAHDFPDVQIILN 186 Query: 184 HLGKPALKEFRKGRAAYERWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQ 243 H G G + W++ + LA P+VACKISG L DW + I Sbjct: 187 HTGMQV-----DGPKHFNAWKKGMHRLAQAPNVACKISG-LGMGDWSWTTAS-----IRP 235 Query: 244 CLDTALELFGPQRLMFGSDWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARI 303 ++ A+ FG R MF +++PV L +S+ VV +E R ++ AL+ +++AR Sbjct: 236 YVEEAIAAFGVGRSMFATNFPVDKLFSSFDAVVNAFKE-ITRYYPHEERLALFHDNAARH 294 Query: 304 YGL 306 Y L Sbjct: 295 YRL 297 >SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 278 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 82/301 (27%), Positives = 132/301 (43%), Gaps = 38/301 (12%) Query: 22 MRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHASITVQARPGHEET 81 M ID H H + +YPW+ G+ L +D L + G+ AS+ ++ E Sbjct: 1 MIIDTHLHLIDKSALNYPWLA-GVPALDRDFLYATYAAEAKRVGVAASLHMEVDVDPAE- 58 Query: 82 GFLLEMARRD-ARIAG--------VIAW---DDIASPQLAERMEQWGRPKMLGLRHPMQD 129 +E+ R+ AR+AG IA +D ER E+ K G R + Sbjct: 59 ---IELETREVARLAGEPGSLLKGAIAACRPEDEGFAAYLERQEENAFVK--GFRRVLHV 113 Query: 130 -EDDAAQALVQPQFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKP 188 DD ++ QP F V+ L + ++ +P+ L L +L+H G P Sbjct: 114 VTDDLSE---QPLFRENVKRLSGTRFTFDLCVLPHQIPKAIALADLAPDVQFILDHCGVP 170 Query: 189 ALKEFRKGRAAYERWRQQLRELAAMPHVACKISGLLT--EADWLRGLCTRDFDHIVQCLD 246 +K G A + WR + E+A P+V KISG++ E DW D I ++ Sbjct: 171 DIK----GHAEHP-WRDHMTEIARHPNVVAKISGVVAYAEEDWA-------LDSIRPYVE 218 Query: 247 TALELFGPQRLMFGSDWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARIYGL 306 + +FG R+++GSDWPVC L + + VA + SP ++ L ++ RI+ L Sbjct: 219 HTISVFGWDRVVWGSDWPVCTLGGNLSTWVAATQA-LIEGCSPQERRKLLSGNAQRIWNL 277 Query: 307 M 307 + Sbjct: 278 I 278 >BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Bme Brucella melitensis Length = 282 Score = 73.2 bits (178), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 81/303 (26%), Positives = 125/303 (41%), Gaps = 39/303 (12%) Query: 22 MRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRL--PYQFQPLLDAQG--LHASITVQARPG 77 M ID H H + YPW+ + L +D L Y+ Q G LH + V Sbjct: 1 MLIDTHLHLIDKNALSYPWLESA-PALNRDFLFDSYRLQAERAGIGGALHIEVDVAPHAM 59 Query: 78 HEETGFLLEMARRDAR-IAGVIAWDDIASPQLAERME-QWGRPKMLGLR---HPMQDEDD 132 ET + ++ARR I G IA P A +E Q P + GLR H + DE Sbjct: 60 QAETDHIRQIARRSGGFIKGAIASCRPEEPGFAAYLERQLSDPFVKGLRRVLHVVPDEVS 119 Query: 133 AAQALVQPQFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPALKE 192 F ++ L+ ++ + + L L +++H G P +K Sbjct: 120 EGAL-----FRENIKRLEGTGLTFDLCVQPHQIGKAIALADLAPDLCFIVDHCGAPDIKA 174 Query: 193 FRKGRAAYERWRQQLRELAAMPHVACKISGLLTEAD---WLRGLCTRDFDHIVQCLDTAL 249 A + WR+ + E++ P+V KISGL+ D W + ++Q Sbjct: 175 -----GALDGWREGMAEISRRPNVIAKISGLVVYCDAESWTVETLRPYAEQVIQS----- 224 Query: 250 ELFGPQRLMFGSDWPVCLLAASYARVVAVVREWAA------RRLSPADQDALWGESSARI 303 FG R+++GSDWPVC LA+S A + W A S ++ L+ E++ RI Sbjct: 225 --FGWDRVIWGSDWPVCTLASSPD---AGLSTWVAATHALLEGCSTTEKSRLFCENARRI 279 Query: 304 YGL 306 + L Sbjct: 280 WNL 282 >AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 297 Score = 72.0 bits (175), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 75/295 (25%), Positives = 134/295 (45%), Gaps = 35/295 (11%) Query: 24 IDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHASITVQARPGHEETGF 83 ID HQH Y W G LA + L++ G+ +I + ETG Sbjct: 21 IDTHQHLILRGKLGYAWT-RGEPSLAGSFTRADYAALVEGSGVIGTIFM-------ETGV 72 Query: 84 LLEMARRDAR-IAGVIA------WDDIAS--PQLAERMEQWGRP----KMLGLRHPMQD- 129 R +AR +AG++ + IAS P+ + + W K++G R +Q Sbjct: 73 DDGDYRAEARLVAGMVGEGTLPMFGQIASCRPETDQGFDAWLEECRHLKVVGFRRILQVM 132 Query: 130 EDDAAQALVQPQFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPA 189 DD ++A + VR + + + +++ H R L +L +L+H G P Sbjct: 133 PDDTSRA---ENYRRNVRKIGKAGWPVDLCFHSRQLAIAAELVRACPDVRFMLDHCGTPD 189 Query: 190 LKEFRKGRAAYERWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTAL 249 + + W +++ +LA++P++ K+SG+ A G T + + ++T L Sbjct: 190 IAG-----NDFPAWSERMADLASLPNLFVKLSGV--TAYCAPGQVT--LESVRPYVETVL 240 Query: 250 ELFGPQRLMFGSDWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARIY 304 +LFGP R+++G DWPV L + +A+ RE+ + LSP +Q ++ ++ R Y Sbjct: 241 KLFGPARMVWGGDWPVVDLGSGLPEWIAMTREFLS-TLSPDEQASIGHGNARRFY 294