BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0023.p (595 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2699 [R] COG1574 Predicted metal-dependent hydrolase with the ... 923 0.0 RSc0239 [R] COG1574 Predicted metal-dependent hydrolase with the... 900 0.0 SMa1819 [R] COG1574 Predicted metal-dependent hydrolase with the... 785 0.0 AGl1745 [R] COG1574 Predicted metal-dependent hydrolase with the... 777 0.0 SMa0380 [R] COG1574 Predicted metal-dependent hydrolase with the... 777 0.0 >PA2699 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Pae Pseudomonas aeruginosa Length = 612 Score = 923 bits (2386), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/569 (79%), Positives = 480/569 (84%), Gaps = 9/569 (1%) Query: 27 DLGGRTVIPGLNDSHLHLIRGGLNYNLELRWEGVPSLADALRMLKDQAARTPHPQWVRVV 86 DL RTVIPGLNDSHLHLIRGGLNYNLELRWEGVPSLADALRML +QA RTP PQWVRVV Sbjct: 53 DLQKRTVIPGLNDSHLHLIRGGLNYNLELRWEGVPSLADALRMLNNQAERTPSPQWVRVV 112 Query: 87 GGWTEFQFAEKRMPTLEELNAAAPDTPVFVLHLYDRALLNRAALQAVGYTKDTPNPPGGE 146 GGW EFQFAEKRMPTLEELN AAPDTPVFVLHLYDRALLNRAAL+ VGYTKDTPNPPGGE Sbjct: 113 GGWNEFQFAEKRMPTLEELNKAAPDTPVFVLHLYDRALLNRAALRVVGYTKDTPNPPGGE 172 Query: 147 IVRDASGNPTGMLIARPNAMILYATLAKGPALPQEYQVNSTRQFMRELNRLGVTSAIDAG 206 IVRD+SG PTGMLIARPNAMILYATLAKGP LP EYQVNSTRQFMRELNRLG+TSAIDAG Sbjct: 173 IVRDSSGEPTGMLIARPNAMILYATLAKGPKLPLEYQVNSTRQFMRELNRLGLTSAIDAG 232 Query: 207 GGFQNYPDDYQIINKLAADGQLTIRIAYNLFTQSKGSELADFQKWTGMVKPGDGDDFLRH 266 GGFQNYPDDYQ+I +LA GQLT+RIAYNLFTQ ELADF+ WTG VK G G DF RH Sbjct: 233 GGFQNYPDDYQVIRELADQGQLTVRIAYNLFTQKPKEELADFKNWTGSVKYGQGSDFFRH 292 Query: 267 NGAGEMLVFSAADFEDFLEPRPDLADGMEEELERVVRHLVSNRWPFRLHATYDESISRML 326 NGAGEMLVFSAADFEDFLEPRPDL ME+ELE VVRHLV RWPFRLHATYDESISRML Sbjct: 293 NGAGEMLVFSAADFEDFLEPRPDLPQTMEQELEPVVRHLVEQRWPFRLHATYDESISRML 352 Query: 327 DVFEKVNRDIPFDGLHWMFDHAETITERNIERVKALGGGIAIQHRMAFQGEYFIDRYGSD 386 DVFEKV+RDIPF+GL W FDHAETIT RNIERV+ALGGGIAIQ RMAFQGEYF+DRYG+ Sbjct: 353 DVFEKVDRDIPFNGLPWFFDHAETITPRNIERVRALGGGIAIQDRMAFQGEYFVDRYGAK 412 Query: 387 AAEHTPPVAKMLEMGVPVGAGTDATRVASYNPWTALYWLVSGRTVGGTQLYRSTGRLPRE 446 AAE TPP+ +ML GVPVGAGTDATRV+SYNPWT+LYWLVSGRTVGG LY LPRE Sbjct: 413 AAEATPPIQRMLAEGVPVGAGTDATRVSSYNPWTSLYWLVSGRTVGGMALYPQG--LPRE 470 Query: 447 TALELWTAGSAWFSSEQAKKGRIQAGQLADLAVLSADFFSVHEDDIKSIESVMTVVDGKV 506 TAL+L+T GSAWFSSEQ KKG+I+ GQLADLA LSADFF V E+ IK IESV+TVVDGKV Sbjct: 471 TALQLFTHGSAWFSSEQGKKGQIKVGQLADLAALSADFFGVEEEAIKWIESVLTVVDGKV 530 Query: 507 VYGTGPFSAHGQGEIPVLPDWSPVAKVPGHYRAVASSDAARQKKTALPHACAGSCGVHGH 566 VY F G PVLPDWSPVAKVPGH+R A TA H C G+C VH H Sbjct: 531 VYAAAEFDKLGPPAPPVLPDWSPVAKVPGHWRQGAP-------LTAQVHQCVGACAVHAH 583 Query: 567 AHDVARKSTVPVSHFAGFWGALGCSCFAF 595 H+ AR S VPVS F GFWGA GCSCFAF Sbjct: 584 EHNRARHSNVPVSDFQGFWGAFGCSCFAF 612 >RSc0239 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Rso Ralstonia solanacearum Length = 612 Score = 900 bits (2325), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/569 (76%), Positives = 484/569 (85%), Gaps = 9/569 (1%) Query: 27 DLGGRTVIPGLNDSHLHLIRGGLNYNLELRWEGVPSLADALRMLKDQAARTPHPQWVRVV 86 DL GRTVIPGLNDSHLHLIRGGLNYNLEL WEGVPSLADALRML++QAARTP PQWVRVV Sbjct: 53 DLRGRTVIPGLNDSHLHLIRGGLNYNLELHWEGVPSLADALRMLREQAARTPSPQWVRVV 112 Query: 87 GGWTEFQFAEKRMPTLEELNAAAPDTPVFVLHLYDRALLNRAALQAVGYTKDTPNPPGGE 146 GGWTEFQFAE+RMPTL+ELN AAPDTPVFVLHLYDRALLNRAAL+ VGYT+DTPNPPGGE Sbjct: 113 GGWTEFQFAERRMPTLDELNQAAPDTPVFVLHLYDRALLNRAALRQVGYTRDTPNPPGGE 172 Query: 147 IVRDASGNPTGMLIARPNAMILYATLAKGPALPQEYQVNSTRQFMRELNRLGVTSAIDAG 206 I RDA G PTGMLIARPNAMILYATLAKGP LP EYQVNSTRQFMRELNRLGVTSAIDAG Sbjct: 173 IQRDAGGEPTGMLIARPNAMILYATLAKGPKLPPEYQVNSTRQFMRELNRLGVTSAIDAG 232 Query: 207 GGFQNYPDDYQIINKLAADGQLTIRIAYNLFTQSKGSELADFQKWTGMVKPGDGDDFLRH 266 GGFQNYP+DY +I +LA + QLT+RIAYNLFTQ ELADF+ WTG V GDDFLRH Sbjct: 233 GGFQNYPEDYTVIRQLAQENQLTVRIAYNLFTQKPKEELADFKHWTGSVPYRQGDDFLRH 292 Query: 267 NGAGEMLVFSAADFEDFLEPRPDLADGMEEELERVVRHLVSNRWPFRLHATYDESISRML 326 NGAGEMLVFSAADFEDFL+PRPDL + ME ELE VVRHLV+ RWPFRLHATYDESISRML Sbjct: 293 NGAGEMLVFSAADFEDFLQPRPDLPETMEPELEGVVRHLVAQRWPFRLHATYDESISRML 352 Query: 327 DVFEKVNRDIPFDGLHWMFDHAETITERNIERVKALGGGIAIQHRMAFQGEYFIDRYGSD 386 DVFE+V+R+IPFDGL W FDHAETI+ +NIERV+ALGGGIAIQ RMAFQGEYF++RYG+ Sbjct: 353 DVFERVDREIPFDGLPWFFDHAETISPKNIERVRALGGGIAIQDRMAFQGEYFVERYGAA 412 Query: 387 AAEHTPPVAKMLEMGVPVGAGTDATRVASYNPWTALYWLVSGRTVGGTQLYRSTGRLPRE 446 AA TPP+ +ML GVPVGAGTDATRV+SYNPWT+LYWLVSGRTVGG LY LPR+ Sbjct: 413 AAGQTPPIQRMLAEGVPVGAGTDATRVSSYNPWTSLYWLVSGRTVGGLPLYPQG--LPRD 470 Query: 447 TALELWTAGSAWFSSEQAKKGRIQAGQLADLAVLSADFFSVHEDDIKSIESVMTVVDGKV 506 AL L+T GSAWFS++Q KG+I+ GQLADLA LSADFFSV E IK+IESV+TVVDG+V Sbjct: 471 VALALYTHGSAWFSADQGNKGQIKVGQLADLAALSADFFSVDEAGIKAIESVLTVVDGRV 530 Query: 507 VYGTGPFSAHGQGEIPVLPDWSPVAKVPGHYRAVASSDAARQKKTALPHACAGSCGVHGH 566 VYG F+ +G IPVLP+WSPVA+VPGH+R A Q++ H CAG+CGVHGH Sbjct: 531 VYGAAEFTEYGPPPIPVLPEWSPVARVPGHWRPAAPL----QRQV---HHCAGACGVHGH 583 Query: 567 AHDVARKSTVPVSHFAGFWGALGCSCFAF 595 AH AR + VP + F FWGA GCSCFAF Sbjct: 584 AHQKARTANVPAADFRAFWGAFGCSCFAF 612 >SMa1819 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Sme Sinorhizobium meliloti Length = 661 Score = 785 bits (2027), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/573 (65%), Positives = 452/573 (78%), Gaps = 6/573 (1%) Query: 27 DLGGRTVIPGLNDSHLHLIRGGLNYNLELRWEGVPSLADALRMLKDQAARTPHPQWVRVV 86 DL G+ V+PGL D+H H++RGGLNYN+ELRW+GV SLADA+ MLK Q A TP PQWVR V Sbjct: 89 DLKGKRVLPGLIDNHTHVVRGGLNYNIELRWDGVRSLADAMDMLKRQVAITPPPQWVRAV 148 Query: 87 GGWTEFQFAEKRMPTLEELNAAAPDTPVFVLHLYDRALLNRAALQAVGYTKDTPNPPGGE 146 GG+TE QF EKR+PT++E+NA APDTPVF+LHLYDRALLN AAL+AVGYTKDTPNPPGGE Sbjct: 149 GGFTEHQFVEKRLPTIDEINAVAPDTPVFLLHLYDRALLNGAALRAVGYTKDTPNPPGGE 208 Query: 147 IVRDASGNPTGMLIARPNAMILYATLAKGPALPQEYQVNSTRQFMRELNRLGVTSAIDAG 206 I+RDASGNPTGML+A+PNA ILY+TLAKGP LP EYQVNSTR FMRELNRLGVT IDAG Sbjct: 209 IIRDASGNPTGMLLAKPNAAILYSTLAKGPKLPFEYQVNSTRHFMRELNRLGVTGVIDAG 268 Query: 207 GGFQNYPDDYQIINKLAADGQLTIRIAYNLFTQSKGSELADFQKWTGMVKPGDGDDFLRH 266 GG+QNYPDDY +I KLA DGQ+T+R+AYNLFTQ E DF WT VK GDD+ RH Sbjct: 269 GGYQNYPDDYAVIQKLADDGQMTVRLAYNLFTQKPKEEKQDFLNWTSSVKYKQGDDYFRH 328 Query: 267 NGAGEMLVFSAADFEDFLEPRPDLADGMEEELERVVRHLVSNRWPFRLHATYDESISRML 326 NGAGEMLVFSAADFEDF +PRPD+ ME +LE VVR L NRWP+R+HATYDE+ISR L Sbjct: 329 NGAGEMLVFSAADFEDFRQPRPDMPPEMEGDLEEVVRVLAENRWPWRMHATYDETISRAL 388 Query: 327 DVFEKVNRDIPFDGLHWMFDHAETITERNIERVKALGGGIAIQHRMAFQGEYFIDRYGSD 386 DVFEKVN+D+P +GL+W FDHAETI+ER+I+R+ ALGGGIA QHRMA+QGEYF++RYG Sbjct: 389 DVFEKVNQDMPLEGLNWFFDHAETISERSIDRIAALGGGIATQHRMAYQGEYFVERYGHG 448 Query: 387 AAEHTPPVAKMLEMGVPVGAGTDATRVASYNPWTALYWLVSGRTVGGTQLYRSTGRLPRE 446 AAE TPP+AKMLE GV V AGTDATRVASYNPW +L W+++G+T+GG QLY L RE Sbjct: 449 AAEATPPIAKMLEKGVHVSAGTDATRVASYNPWVSLSWMITGKTLGGMQLYPRANCLDRE 508 Query: 447 TALELWTAGSAWFSSEQAKKGRIQAGQLADLAVLSADFFSVHEDDIKSIESVMTVVDGKV 506 TAL +WT WFS+E+ KKGRI+ GQ ADL V DFF+ ED+I I S +T+V GK+ Sbjct: 509 TALRMWTENVTWFSNEEGKKGRIEKGQFADLIVPDKDFFACPEDEISFITSELTMVGGKI 568 Query: 507 VYGTGPFSAHGQGEI-PVLPDWSPVAKVPGHYRAVASSDAARQ---KKTALPHA-CAGSC 561 VYGTG F+ + ++ P +PDWSPV ++ G Y A + A + ++TA+ CA +C Sbjct: 569 VYGTGTFADFNENDVPPAMPDWSPV-RMFGGYAAWGEPEGAGKRSLRRTAMATCGCASNC 627 Query: 562 GVHGHAHDVARKSTVPVSHFAGFWGALGCSCFA 594 VHGH H A S +P+S GF+GALGCSC+A Sbjct: 628 NVHGHDHAGAWTSKLPISDVKGFFGALGCSCWA 660 >AGl1745 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 625 Score = 777 bits (2007), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/573 (65%), Positives = 446/573 (77%), Gaps = 6/573 (1%) Query: 27 DLGGRTVIPGLNDSHLHLIRGGLNYNLELRWEGVPSLADALRMLKDQAARTPHPQWVRVV 86 DL G+ V+PGL D+H H+IRGGLN+N+ELRW+GV SLADA+ MLK Q A TP PQWVRVV Sbjct: 53 DLKGKRVLPGLIDNHTHVIRGGLNFNMELRWDGVRSLADAMYMLKRQVAITPAPQWVRVV 112 Query: 87 GGWTEFQFAEKRMPTLEELNAAAPDTPVFVLHLYDRALLNRAALQAVGYTKDTPNPPGGE 146 GG+TE QF EKR+PT+EE+NA APDTPVF+LHLYDRALLN AAL+AVGYTKDTPNPPGGE Sbjct: 113 GGFTEHQFVEKRLPTIEEINAVAPDTPVFLLHLYDRALLNGAALRAVGYTKDTPNPPGGE 172 Query: 147 IVRDASGNPTGMLIARPNAMILYATLAKGPALPQEYQVNSTRQFMRELNRLGVTSAIDAG 206 I RDA+GNPTG+L+A+PNA ILY+TLAKGP LP +YQVNSTR FMRELNRLGVT IDAG Sbjct: 173 ITRDANGNPTGLLLAKPNAGILYSTLAKGPKLPLDYQVNSTRHFMRELNRLGVTGVIDAG 232 Query: 207 GGFQNYPDDYQIINKLAADGQLTIRIAYNLFTQSKGSELADFQKWTGMVKPGDGDDFLRH 266 GGFQNYPDDY++I KL+ + Q+T+R+AYNLFTQ E DF WT VK G+D+ RH Sbjct: 233 GGFQNYPDDYEVIQKLSDENQMTVRLAYNLFTQKPKQEKEDFLNWTSSVKYKQGNDYFRH 292 Query: 267 NGAGEMLVFSAADFEDFLEPRPDLADGMEEELERVVRHLVSNRWPFRLHATYDESISRML 326 NGAGEMLVFSAADFEDF +PRPD+ ME ELE VVR L NRWP+RLHATYDE+ISR L Sbjct: 293 NGAGEMLVFSAADFEDFRQPRPDMPPEMEGELEDVVRILAENRWPWRLHATYDETISRAL 352 Query: 327 DVFEKVNRDIPFDGLHWMFDHAETITERNIERVKALGGGIAIQHRMAFQGEYFIDRYGSD 386 DVFEKVNRDIP +GL+W FDHAETI++R+I+R+ ALGGGIA QHRMA+QGEYF++RYG Sbjct: 353 DVFEKVNRDIPLEGLNWFFDHAETISDRSIDRIAALGGGIATQHRMAYQGEYFVERYGQG 412 Query: 387 AAEHTPPVAKMLEMGVPVGAGTDATRVASYNPWTALYWLVSGRTVGGTQLYRSTGRLPRE 446 AE TPP+A+MLE GV V AGTDATRVASYNPW +L+W+V+G+TVGG QLY L RE Sbjct: 413 VAEATPPIARMLEKGVNVSAGTDATRVASYNPWVSLFWMVTGKTVGGMQLYPRANCLDRE 472 Query: 447 TALELWTAGSAWFSSEQAKKGRIQAGQLADLAVLSADFFSVHEDDIKSIESVMTVVDGKV 506 TAL +WT WFS+E+ KKGRI+ GQ ADL V D+FS ED+I + S +T+V GK+ Sbjct: 473 TALRMWTEKVTWFSNEEGKKGRIEKGQFADLVVPDKDYFSCAEDEISFLTSELTMVGGKI 532 Query: 507 VYGTGPFSAHGQGEI-PVLPDWSPVAKVPGHYRAVASSDAARQ---KKTALPHA-CAGSC 561 VYG G F + I P +PDWSPV + G Y A A + ++ A+ CA C Sbjct: 533 VYGAGDFRVLDESNIPPAMPDWSPV-RTFGGYAAWGEPQGAGKNSLRRAAITSCGCASDC 591 Query: 562 GVHGHAHDVARKSTVPVSHFAGFWGALGCSCFA 594 GVHGH H A S +P++ GF+GALGCSC+A Sbjct: 592 GVHGHDHAGAWTSKLPIADLKGFFGALGCSCWA 624 >SMa0380 [R] COG1574 Predicted metal-dependent hydrolase with the TIM-barrel fold Sme Sinorhizobium meliloti Length = 660 Score = 777 bits (2007), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/572 (65%), Positives = 447/572 (78%), Gaps = 4/572 (0%) Query: 27 DLGGRTVIPGLNDSHLHLIRGGLNYNLELRWEGVPSLADALRMLKDQAARTPHPQWVRVV 86 DL G+ V+PGL D+H H++RGGLNYN+ELRW+GV SLADA+ MLK Q A TP PQWVRVV Sbjct: 88 DLKGKGVLPGLIDNHTHVVRGGLNYNMELRWDGVRSLADAMDMLKRQVAITPAPQWVRVV 147 Query: 87 GGWTEFQFAEKRMPTLEELNAAAPDTPVFVLHLYDRALLNRAALQAVGYTKDTPNPPGGE 146 GG+TE QFAEKR+PT+EE+NA APDTPVF+LHLYDRALLN AAL+AVGYT+DTPNPPGGE Sbjct: 148 GGFTEHQFAEKRLPTIEEINAVAPDTPVFLLHLYDRALLNGAALRAVGYTRDTPNPPGGE 207 Query: 147 IVRDASGNPTGMLIARPNAMILYATLAKGPALPQEYQVNSTRQFMRELNRLGVTSAIDAG 206 I RDA+GNPTG+L+A+PNA ILY+TLAKGP LP +YQVNSTR FMRELNRLGVT IDAG Sbjct: 208 ITRDANGNPTGLLLAKPNAGILYSTLAKGPKLPLDYQVNSTRHFMRELNRLGVTGVIDAG 267 Query: 207 GGFQNYPDDYQIINKLAADGQLTIRIAYNLFTQSKGSELADFQKWTGMVKPGDGDDFLRH 266 GGFQNYPDDY++I KL+ + Q+T+R+AYNLFTQ E DF WT VK G+D+ RH Sbjct: 268 GGFQNYPDDYEVIQKLSDENQMTVRLAYNLFTQKPKEEKQDFLNWTQSVKYKQGNDYFRH 327 Query: 267 NGAGEMLVFSAADFEDFLEPRPDLADGMEEELERVVRHLVSNRWPFRLHATYDESISRML 326 NGAGEMLVFSAADFEDF +PRP++A ME ELE V+R L NRWP+RLHATYDE+ISR L Sbjct: 328 NGAGEMLVFSAADFEDFRQPRPEMAPEMEGELEEVLRVLAENRWPWRLHATYDETISRAL 387 Query: 327 DVFEKVNRDIPFDGLHWMFDHAETITERNIERVKALGGGIAIQHRMAFQGEYFIDRYGSD 386 DVFEKVN+DIP +GL+W FDHAETI++R+I+R+ ALGGGIA QHRMA+QGEYF +RYG Sbjct: 388 DVFEKVNKDIPLEGLNWFFDHAETISDRSIDRIAALGGGIATQHRMAYQGEYFAERYGHG 447 Query: 387 AAEHTPPVAKMLEMGVPVGAGTDATRVASYNPWTALYWLVSGRTVGGTQLYRSTGRLPRE 446 AE TPP+ +ML+ GV V AGTDATRVASYNPW +L W+V+G+TVGG QLY L RE Sbjct: 448 VAEATPPIRRMLDKGVNVSAGTDATRVASYNPWVSLSWMVTGKTVGGMQLYPRANCLDRE 507 Query: 447 TALELWTAGSAWFSSEQAKKGRIQAGQLADLAVLSADFFSVHEDDIKSIESVMTVVDGKV 506 TAL +WT WFS+E+ KKGRI+ GQ ADL V DFFS ED+I I S +T+V GK+ Sbjct: 508 TALRMWTEKVTWFSNEEGKKGRIEKGQFADLVVPDKDFFSCAEDEISFIVSELTMVGGKI 567 Query: 507 VYGTGPFSAHGQGEI-PVLPDWSPVAKVPGH--YRAVASSDAARQKKTALPHA-CAGSCG 562 VYG G F + EI P +PDWSPV K G+ + + A ++TA+ CA CG Sbjct: 568 VYGAGDFKTLDENEIPPAMPDWSPVRKFGGYAAWGEPERAGARSLRRTAISTCGCASDCG 627 Query: 563 VHGHAHDVARKSTVPVSHFAGFWGALGCSCFA 594 VHGH H A S +P++ GF+GALGCSC+A Sbjct: 628 VHGHDHAGAWTSKLPIADLKGFFGALGCSCWA 659