BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0010.p (323 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5386 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Pae Pseudomon... 293 3e-79 AF1206 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Afu Archaeogl... 242 1e-63 SSO0647 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Sso Sulfolob... 206 4e-53 SMc01638_1 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Sme Sinor... 201 2e-51 mlr5041 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Mlo Mesorhiz... 199 8e-51 >PA5386 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Pae Pseudomonas aeruginosa Length = 321 Score = 293 bits (751), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 152/307 (49%), Positives = 204/307 (66%) Query: 14 EVRRVAVVGTGVIGASWAAYFLARGLEVSATDPMPDAEVKLRAAVARHWPSLELLGLATG 73 E++ A +G+GVIG+ W A LA GL+V A DP P AE LRA VA WP+L GLA G Sbjct: 6 EIKTFAALGSGVIGSGWIARALAHGLDVVAWDPAPGAEAALRARVANAWPALRKQGLAPG 65 Query: 74 ASPERLRFSASLEEALEGADFVQENGPEREDLKIDLFRRMDAALPAHVVLASSSSGLLMS 133 A+ ERLRF AS+EE + ADF+QE+ PER DLK+DL R+ AA V++ SS+SGLL S Sbjct: 66 AAQERLRFVASIEECVGDADFIQESAPERLDLKLDLHARISAAARPDVLIGSSTSGLLPS 125 Query: 134 KVQAACRHPGRVVLGHPFNPPHLIPLVEVIGGAQTAPEAVQAALDFYTAIGKKAIHVRKE 193 + A HP R ++GHPFNP +L+PLVEV+GG +TA EAV+AA+ Y ++G + +HVRKE Sbjct: 126 EFYAEASHPERCLVGHPFNPVYLLPLVEVVGGERTAAEAVRAAMRVYESLGMRPLHVRKE 185 Query: 194 VKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPYLNLHMSGGEGG 253 V G IA+RL ALWREA HLV GVA+T +ID AI +G GLRW+ MG +L ++GG G Sbjct: 186 VPGFIADRLLEALWREALHLVNDGVATTGEIDDAIRFGAGLRWSFMGTFLTYTLAGGNAG 245 Query: 254 IGHMLDHLGPPIESWWADLGAPSITAALKAQIRAGVEEQMSGKTSAGIAAERDQLLVQLL 313 + H + GP ++ W L AP +T AL ++ G EQ ++ A + RD L+ +L Sbjct: 246 MRHFMAQFGPALQLPWTYLPAPELTEALIDRVVEGTAEQQGARSIAELERYRDDCLLAVL 305 Query: 314 AAKSKTQ 320 A +T+ Sbjct: 306 GAIRETK 312 >AF1206 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Afu Archaeoglobus fulgidus Length = 315 Score = 242 bits (617), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 129/310 (41%), Positives = 191/310 (61%), Gaps = 18/310 (5%) Query: 17 RVAVVGTGVIGASWAAYFLARGLEVSATDPMPDAEVKLRAAVARHWPSLELLGLATGASP 76 +VA +G G +GASWA+ F RG +V+ DP P+A + A++AR +L + +G+ Sbjct: 4 KVACIGAGTVGASWASLFAWRGCDVAVYDPFPEALNRAEASIARTVSTLS--EIFSGSED 61 Query: 77 E------RLRFSASLEEALEGADFVQENGPEREDLKIDLFRRMDAALPAHVVLASSSSGL 130 + R++F+ +LEEAL+GA +VQE+ E+ ++K DLF +MDA +LA+S+SGL Sbjct: 62 DVKSALSRVKFTENLEEALKGAYYVQESAVEKLEVKRDLFEKMDAIAEPETILATSTSGL 121 Query: 131 LMSKVQAACR-HPGRVVLGHPFNPPHLIPLVEVIGGAQTAPEAVQAALDFYTAIGKKAIH 189 +S++Q A R HP R + HP+NPPHLIPLVEV+ QT + ++F +GKK I Sbjct: 122 SISEIQTAARKHPERCITAHPYNPPHLIPLVEVVPRKQTDESCTEKTVEFMERMGKKPIV 181 Query: 190 VRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPYLNLHMSG 249 V+K+V G +ANRL AALWREA +LV QG+A+ E+ID A+ YGPG+RWA+ G YL H+ G Sbjct: 182 VKKDVPGMVANRLAAALWREAVNLVYQGIATPEEIDVAVKYGPGIRWAITGVYLTYHLGG 241 Query: 250 GEGGIGHMLDHLGPPIESWWADLG------APSITAALKAQIRAGVEEQMSGKTSAGIAA 303 G+GG+ + L+ W+DL S A LK+ + ++MS +A Sbjct: 242 GQGGMKYFLEFFDDHYRRLWSDLANWSEYPEGSFDAILKSMEGYDIIKRMSYDE---LAK 298 Query: 304 ERDQLLVQLL 313 RD L ++L Sbjct: 299 WRDDQLAKIL 308 >SSO0647 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Sso Sulfolobus solfataricus Length = 324 Score = 206 bits (525), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 119/317 (37%), Positives = 175/317 (55%), Gaps = 10/317 (3%) Query: 13 QEVRRVAVVGTGVIGASWAAYFLARGLEVSATDPMPDAEVKLRAAVARHWPSLELLGLAT 72 + + +VAV+G GVIG W LA+G +V+ + K A V+ + +L+ LG+ Sbjct: 6 KSINKVAVIGAGVIGVGWTTLLLAKGYKVNLYTEKKETLEKALAKVSAYLVNLKNLGM-I 64 Query: 73 GASPE----RLRFSASLEEALEGADFVQENGPEREDLKIDLFRRMDAALPAHVVLASSSS 128 PE L +++A+ DFV E E K +LF+ +D LP +++ASS+S Sbjct: 65 NEEPESYITNLTGITKIDDAIHNVDFVIEAIIEDYTAKKNLFKLLDTQLPQDIIIASSTS 124 Query: 129 GLLMSKVQAA-CRHPGRVVLGHPFNPPHLIPLVEVIGGAQTAPEAVQAALDFYTAIGKKA 187 GLLM+++Q A RHP R V+ HP+NPPHL+PLVE++ G +T+ E V +F + + Sbjct: 125 GLLMTEIQKAMIRHPERGVIAHPWNPPHLLPLVEIVPGEKTSKETVDLTREFMEKLDRVV 184 Query: 188 IHVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPYLNLHM 247 + +RKEV G I NRL AL+REA +LV++GVA+ EDID + GLRWA MGP+L H+ Sbjct: 185 VLLRKEVPGFIGNRLAFALFREAVNLVDEGVATVEDIDKVMTAAIGLRWAFMGPFLTYHL 244 Query: 248 SGGEGGIGHMLDH-LGPPIESWWADLGAPS---ITAALKAQIRAGVEEQMSGKTSAGIAA 303 GGEGGI + G W L TA K + E + GK+ ++ Sbjct: 245 GGGEGGIEYFFSKGFGYGANEWMHTLAKWDKFPYTAVKKVLEQMKEYEFIKGKSFQDLSR 304 Query: 304 ERDQLLVQLLAAKSKTQ 320 RD+ LV + A K + Sbjct: 305 WRDEKLVTIYKAVYKEK 321 >SMc01638_1 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Sme Sinorhizobium meliloti Length = 351 Score = 201 bits (510), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 108/302 (35%), Positives = 175/302 (57%), Gaps = 1/302 (0%) Query: 15 VRRVAVVGTGVIGASWAAYFLARGLEVSATDPMPDAEVKLRAAVARHWPSLELLGLATGA 74 + + A +G GVIG +WAA F G++V+ DP P+AE + +A + +L +A Sbjct: 4 ITKAACIGGGVIGGAWAARFALAGIDVNIFDPHPEAERIIGEVMANAERAYGMLTMAPLP 63 Query: 75 SPERLRFSASLEEALEGADFVQENGPEREDLKIDLFRRMDAALPAHVVLASSSSGLLMSK 134 + F S++EA+EG D++QE+ PER LK + +DAA ++ SS+SGLL S Sbjct: 64 PRGKFTFCRSIQEAVEGVDWIQESVPERLPLKRGVINEIDAAARPDALIGSSTSGLLPSD 123 Query: 135 VQAACRHPGRVVLGHPFNPPHLIPLVEVIGGAQTAPEAVQAALDFYTAIGKKAIHVRKEV 194 +QA +HP R+ + HP+NP +L+PLVE++GG +T+PE ++ A + IG K + + KE+ Sbjct: 124 LQAEMKHPERMFVAHPYNPVYLLPLVELVGGRKTSPETIRRAEEAVAEIGMKGVVIAKEI 183 Query: 195 KGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPYLNLHMSGGEGGI 254 + + +RL ALWREA L++ + TE +D + Y G+RWA MG + ++GGE G+ Sbjct: 184 EAFVGDRLLEALWREALWLIQDDICDTETLDDVMRYSFGMRWAQMGLFETYRIAGGEAGM 243 Query: 255 GHMLDHLGPPIESWWADL-GAPSITAALKAQIRAGVEEQMSGKTSAGIAAERDQLLVQLL 313 H L GP ++ W + AL +I A + Q +G++ + RD+ LV ++ Sbjct: 244 RHFLAQFGPCLKWPWTKFTDVVDLDDALVEKIGAQSDAQAAGRSIRELERIRDENLVGIM 303 Query: 314 AA 315 A Sbjct: 304 HA 305 >mlr5041 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Mlo Mesorhizobium loti Length = 364 Score = 199 bits (506), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 110/294 (37%), Positives = 170/294 (57%), Gaps = 8/294 (2%) Query: 30 WAAYFLARGLEVSATDPMPDAEVKLRAAVA---RHWPSLELLGLATGASPERLRFSASLE 86 W A L G++VS DP P+A K+ + R + + GL +L F+ ++ Sbjct: 19 WVARLLLNGIDVSIFDPDPEASRKVSEVMKGARRAYKQMVPGGLPKEG---KLTFAKTIA 75 Query: 87 EALEGADFVQENGPEREDLKIDLFRRMDAALPAHVVLASSSSGLLMSKVQAACR-HPGRV 145 EA+ ADF+QE+ PER DLK + +DA PA+ ++ SS+SG+ + +Q A + HP R+ Sbjct: 76 EAVADADFIQESVPERLDLKHRVLAEIDAHAPANAIVGSSTSGIKPTDMQVAMKKHPERL 135 Query: 146 VLGHPFNPPHLIPLVEVIGGAQTAPEAVQAALDFYTAIGKKAIHVRKEVKGHIANRLQAA 205 V+GHPFNP +L+PLVE++GG QT PEA++ A + Y +IG K + +RKE++ + +RL A Sbjct: 136 VVGHPFNPVYLLPLVEIVGGDQTFPEAIEVAKEIYASIGMKPVVIRKEIEAFVGDRLLEA 195 Query: 206 LWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPYLNLHMSGGEGGIGHMLDHLGPPI 265 WREA L++ G+ + E++D + YG GLRWA MG + ++GGE G+ H + GP + Sbjct: 196 AWREALWLIKDGICTVEELDDIMRYGFGLRWAQMGMFQVYRVAGGEAGMRHFMAQFGPCL 255 Query: 266 ESWWADL-GAPSITAALKAQIRAGVEEQMSGKTSAGIAAERDQLLVQLLAAKSK 318 + W L P L I ++Q G + + RD LV ++ A SK Sbjct: 256 KWPWTKLMDVPEFNDELVDLIATQSDDQAHGLSIRELEKIRDDNLVAIMDALSK 309