BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0256.p (242 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5281 [R] COG1011 Predicted hydrolase (HAD superfamily) Pae Pse... 134 2e-31 STM3950 [R] COG1011 Predicted hydrolase (HAD superfamily) Sty Sa... 92 9e-19 VC0129 [R] COG1011 Predicted hydrolase (HAD superfamily) Vch Vib... 89 9e-18 PM1700 [R] COG1011 Predicted hydrolase (HAD superfamily) Pmu Pas... 84 4e-16 ECs4742 [R] COG1011 Predicted hydrolase (HAD superfamily) Ecs Es... 83 5e-16 >PA5281 [R] COG1011 Predicted hydrolase (HAD superfamily) Pae Pseudomonas aeruginosa Length = 232 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 86/225 (38%), Positives = 120/225 (53%), Gaps = 7/225 (3%) Query: 15 SPIEAVLFDLDDTLWAIEPVLVRAEGLLYDWLREHAPAVLAAHTIASLRERRMALMQTDP 74 S + V FDLDDTLW + PV+ AE LL +WL +A A L I L R L+ +P Sbjct: 2 SSLRLVTFDLDDTLWDVAPVMNNAEALLREWLASNA-ARLGPVPIEHLWAIRTRLLDREP 60 Query: 75 MYRIDLWKLR-----HTALSEVFREHDVDLALVDPAMALFSEARSTVALFDDVEPALLRM 129 M R L +LR H L + + + + +L + +F EAR V LF +V P L + Sbjct: 61 MLRHRLSELRRRILFHALLDAGYPQAEAE-SLAEAGFQVFLEARHRVTLFPEVHPTLEIL 119 Query: 130 KGRLTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPAATVY 189 R TLG ++NG AD+ R+GLA +F ++ A G KPDP F A + V +A ++ Sbjct: 120 ADRFTLGVLTNGNADVRRLGLADYFRFALCAEELGVGKPDPTPFREALKRAGVEASAAIH 179 Query: 190 VGDDPLLDVQGAQRAGLRAVWMNRFDRTLPEGITPDAICRDLHEL 234 +GD P D+ GA+RAG+RA+W N + P A R L EL Sbjct: 180 IGDHPSDDIAGARRAGMRAIWFNPSGKPWAGEEEPSAEIRSLAEL 224 >STM3950 [R] COG1011 Predicted hydrolase (HAD superfamily) Sty Salmonella typhimurium LT2 Length = 238 Score = 92.4 bits (228), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 59/200 (29%), Positives = 103/200 (51%), Gaps = 5/200 (2%) Query: 17 IEAVLFDLDDTLWAIEPVLVRAEGLLYDWLREHAPAVLAAHTIASLRERRMALMQTDPMY 76 I A+ FDLDDTL+ PV++R E +++ + P++ + + L+ R A+ + +P Sbjct: 10 IAALTFDLDDTLYDNRPVILRTEQEALAFMQNYHPSLRSFQNV-DLQRIRQAVREAEPEI 68 Query: 77 RIDLWKLRHTALSEVFREHDVD----LALVDPAMALFSEARSTVALFDDVEPALLRMKGR 132 D+ + RH A+ + R+ + +A + AM F++ RS + + L ++ + Sbjct: 69 YHDVTRWRHRAIEQAMRDAGLSAQEAIAGANAAMMHFAKWRSQIEVPQATHETLQQLAKK 128 Query: 133 LTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPAATVYVGD 192 L +++NG A E GL +F + A GR+KP ++ A E L V ++VGD Sbjct: 129 WPLVAITNGNAQPELFGLGDYFKFVLRAGPDGRSKPFSDMYFLAAEKLHVPIGEILHVGD 188 Query: 193 DPLLDVQGAQRAGLRAVWMN 212 D DV GA R G++A W+ Sbjct: 189 DLTTDVAGAIRCGMQACWIK 208 >VC0129 [R] COG1011 Predicted hydrolase (HAD superfamily) Vch Vibrio cholerae Length = 239 Score = 89.0 bits (219), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 65/233 (27%), Positives = 112/233 (48%), Gaps = 23/233 (9%) Query: 17 IEAVLFDLDDTLWAIEPVLVRAEGLLYDWLREHAPAVLAAHTIASLR--------ERRMA 68 I+A+ FDLDDTL+ PV+ + E + +WL L+ H I + R +R +A Sbjct: 10 IQALTFDLDDTLYDNRPVIKQVEEKVTEWL-------LSEHPITATRPLAWWLAMKRDIA 62 Query: 69 LMQTDPMYRIDLWK---LRHTALSEVFREHDVDLALVDPAMALFSEARSTVALFDDVEPA 125 + + + W+ ++H L + + + + A + R+ V + + Sbjct: 63 RRFPEQCHDVSQWRYLQVQHGLLELGYAQPEAEQA-ASETLEQVMRWRNQVDVPAETHRV 121 Query: 126 LLRMKGRLTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPA 185 L ++ ++ L +++NG +E+IGL+G+F + A GRAKP P +F A + LQ+ P Sbjct: 122 LAQLAAKVPLIAITNGNVQIEKIGLSGYFQTVLRAGPDGRAKPYPDLFAQAAQQLQLEPR 181 Query: 186 ATVYVGDDPLLDVQGAQRAGLRAVWMN----RFDRTLPEGITPDAICRDLHEL 234 + ++VGD DV GA++ G +A W N R + PD L EL Sbjct: 182 SILHVGDHLQTDVLGARQNGFQACWFNDQGQSIRRLAKASVLPDVEIERLSEL 234 >PM1700 [R] COG1011 Predicted hydrolase (HAD superfamily) Pmu Pasteurella multocida Length = 236 Score = 83.6 bits (205), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 61/213 (28%), Positives = 105/213 (49%), Gaps = 11/213 (5%) Query: 16 PIEAVLFDLDDTLWAIEPVLVRAEGLLYDWLREHAPAVLAAHTIASLRERRMALMQTDPM 75 P + + FDLDDTL+ V+ AE + ++ + +++ + ++R + Q PM Sbjct: 9 PFKLISFDLDDTLYDNSDVIRLAEENFIEKVKLESQLNISSEEWRAWKQR---IEQRHPM 65 Query: 76 YRIDLWKLRHTALSEVFREHDVDLALVD----PAMALFSEARSTVALFDDVEPALLRMKG 131 D+ R L ++ + A ++ AMALF E R + + + L +K Sbjct: 66 LCEDVVAWRIETLHQLLANYQKSAAEIERVCQQAMALFVEWRHKIDVPVQSQQVLNLLKQ 125 Query: 132 RLTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPAATVYVG 191 + L +++NG + +RIGL+ F + + GRAKP +FH + V P ++VG Sbjct: 126 KYPLVAITNGNVEPQRIGLS-QFDLVLRGGEQGRAKPHQDLFHQTAQHFGVQPHEILHVG 184 Query: 192 DDPLLDVQGAQRAGLRAVWMN---RFDRTLPEG 221 D+ + DVQGA +A +AVW+N + R PE Sbjct: 185 DNLMTDVQGAIQADCQAVWINLSGKVLRDFPEA 217 >ECs4742 [R] COG1011 Predicted hydrolase (HAD superfamily) Ecs Escherichia coli O157:H7 Length = 238 Score = 83.2 bits (204), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 58/199 (29%), Positives = 99/199 (49%), Gaps = 5/199 (2%) Query: 17 IEAVLFDLDDTLWAIEPVLVRAEGLLYDWLREHAPAVLAAHTIASLRERRMALMQTDPMY 76 I A+ FDLDDTL+ PV++R E +++ + PA L + L+ R A+ + +P Sbjct: 10 ISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHPA-LRSFQNEDLQRLRQAVREAEPEI 68 Query: 77 RIDLWKLRHTALSEVFREHDVDL----ALVDPAMALFSEARSTVALFDDVEPALLRMKGR 132 D+ + R ++ + + + A AM F++ RS + + L ++ + Sbjct: 69 YHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAMINFAKWRSRIDVPQQTHDTLKQLAKK 128 Query: 133 LTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPAATVYVGD 192 L +++NG A E GL +F + A GR+KP ++ A E L V ++VGD Sbjct: 129 WPLVAITNGNAQPELFGLGDYFEFVLRAGPHGRSKPFSDMYFLAAEKLNVPIGEILHVGD 188 Query: 193 DPLLDVQGAQRAGLRAVWM 211 D DV GA R+G++A W+ Sbjct: 189 DLTTDVGGAIRSGMQACWI 207