BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0208.p (484 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0463 [ER] COG0591 Na+/proline symporter Rso Ralstonia solanac... 593 e-169 PA1418 [ER] COG0591 Na+/proline symporter Pae Pseudomonas aerugi... 112 2e-24 PA0287 [ER] COG0591 Na+/proline symporter Pae Pseudomonas aerugi... 103 6e-22 STM3382 [H] COG4145 Na+/panthothenate symporter Sty Salmonella t... 91 5e-18 panF [H] COG4145 Na+/panthothenate symporter Eco Escherichia col... 91 8e-18 >RSc0463 [ER] COG0591 Na+/proline symporter Rso Ralstonia solanacearum Length = 479 Score = 593 bits (1528), Expect = e-169, Method: Compositional matrix adjust. Identities = 302/463 (65%), Positives = 357/463 (77%), Gaps = 27/463 (5%) Query: 30 LLWFVVLYWVISVGIGLYATRYVKSSKDYAVAGRSLPMSVVTATVFATWFGSEAVLGVSS 89 L+WFV++YWVISVGIGL+A V+++ D+AVAGR LP VVTATVFATWFGSE VLG+ + Sbjct: 2 LIWFVIVYWVISVGIGLWAALRVRNTADFAVAGRGLPFYVVTATVFATWFGSETVLGIPA 61 Query: 90 TFLKDGLGGIVADPFGSSLCLILVGVFFAKKLYRMNLLTIGDYYKKRFGRAAEMLVTICI 149 FLK+GL G+VADPFGSSLCLILVG+FFA+ LYRMNLLTIGD+Y+ RFGR AE+L T+CI Sbjct: 62 VFLKEGLHGVVADPFGSSLCLILVGLFFARPLYRMNLLTIGDFYRNRFGRVAEVLTTLCI 121 Query: 150 VISYLGWVAAQIKALGLVFNVVSGGYIPMHWGMIIGAGSVLIYTLMGGMWAVAITDFLQM 209 V+SYLGWVAAQIKALGLVF VS G + GM+IGA SVL+YTL GGMW+VA+TDF+QM Sbjct: 122 VVSYLGWVAAQIKALGLVFYTVSDGGLSQQTGMMIGAASVLVYTLFGGMWSVAVTDFIQM 181 Query: 210 IIVVVGMLYIGYEVSGMVGGPGVVIAHAAEAGKFNFWPAAELKDVLWFFAAWITMMLGSI 269 II+V+GM+YIG+EVSG GG V+AHA+ AGKF+FWPA +V+ F AWITMMLGSI Sbjct: 182 IIIVIGMMYIGWEVSGQAGGVATVVAHASAAGKFSFWPAFNPIEVIGFVTAWITMMLGSI 241 Query: 270 PQQDVFQRVMSSKNENVARNASVLGGVIYFFFVFIPIFLAYSATLIDPAMVSRLLETDSQ 329 PQQDVFQRV SS+ E +A ASVLGGV+YF F FIP+FLAYSATLIDP MV+R + TDSQ Sbjct: 242 PQQDVFQRVTSSRTERIAGTASVLGGVLYFLFAFIPMFLAYSATLIDPQMVARYINTDSQ 301 Query: 330 LVLPTLIMEKMPVFAQVMFFGALLSAIKSCASATLLAPSVTFTENVLREIRPWKSDREFL 389 L+LP L++E P+ AQVMFFGALLSAIKSCASATLLAPSVTF ENVLR + P D+ FL Sbjct: 302 LILPKLVLEHAPLVAQVMFFGALLSAIKSCASATLLAPSVTFAENVLRPMLPRMDDKRFL 361 Query: 390 FSMRAVVLVFTVGVT---------------------------TLAFGVYWQRATRQGALM 422 M+AVVLVFT VT LAFG++W+RATRQG L+ Sbjct: 362 RVMQAVVLVFTALVTLFALNSHLSIFHMVENAYKVTLVAAFVPLAFGLFWKRATRQGGLL 421 Query: 423 AMVLGLVSWITLEYAAPDGLWPPQLAGLLFSIGGMVVGSLMPQ 465 A+ LGL WI E D ++PPQL GLL S+G MVVGSL PQ Sbjct: 422 AIALGLTVWIWCEVFLSDAMFPPQLVGLLASLGAMVVGSLGPQ 464 >PA1418 [ER] COG0591 Na+/proline symporter Pae Pseudomonas aeruginosa Length = 463 Score = 112 bits (280), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 115/448 (25%), Positives = 207/448 (46%), Gaps = 54/448 (12%) Query: 29 ALLWFVVLYWVIS-VGIGLYATRYVKSSKDYAVAGRSLPMSVVTATVFATWFGSEAVLGV 87 AL FVVL + + +G Y R K+ DY VAGR+L T+ AT G + +G Sbjct: 2 ALDIFVVLIYAAGMIALGWYGMRRAKTRDDYLVAGRNLGPGFYLGTMAATVLGGASTIGT 61 Query: 88 SSTFLKDGLGGI-VADPFGSSLCLILVGVFFAKKLYRMNLLTIGDYYKKRFGRAAEMLVT 146 G+ G + G L ++ + +F AK L ++ + T+ ++R+ AA Sbjct: 62 VRLGYVHGISGFWLCGAIG--LGIVGLSLFLAKPLLKLKIYTVTQVLERRYNPAARHASA 119 Query: 147 ICIVISYLGWVAAQIKALGLVFNVVSGGYIPMHWGMIIGAGSVLIYTLMGGMWAVAITDF 206 + +++ L A A+G V V+ G +P ++IG G V++Y+ +GGMW++ +TD Sbjct: 120 LIMLVYALMIGATSTIAIGTVMQVLFG--LPFWVSILIGGGVVVLYSTIGGMWSLTLTDI 177 Query: 207 LQMIIVVVGMLYIGYEVSGMVGGPGVVIAHAAEAGKFNF----WPAAELKDVLWFFAAWI 262 +Q +I+ VG++++ +S G + A F+F W +++FF +I Sbjct: 178 VQFLIMTVGLVFLLMPLSINDAGGWDALVAKLPASYFDFTAIGWDTIVTYFLIYFFGIFI 237 Query: 263 TMMLGSIPQQDVFQRVMSSKNENVARNASVLGGVIYFFFVFIPIFLAYSATLIDPAMVSR 322 QD++QRV ++++E VA+ A G+ + + +A ++ P + Sbjct: 238 --------GQDIWQRVFTARSETVAKVAGSAAGIYCVLYGMAGALIGMAAKVLLPDL--- 286 Query: 323 LLETDSQLVLPTLIMEKMPVFAQVMFFGALLSAIKSCASATLLAPSVTFTENVLREIRPW 382 + +++ +P + + A L+A+ S ASA LLA S T T+++L +R Sbjct: 287 ---ENVNNAFASVVEHSLPNGIRGLVIAAALAALMSTASAGLLAASTTVTQDLLPRLRRG 343 Query: 383 K--SDREFLFSMR-------------AVVLVFTVGVTTLAFG-------------VYWQR 414 + SD + R A+V+ + T+A+ +YW+R Sbjct: 344 RGQSDNGDVHENRIATLLLGLVVLGIALVVSDVISALTVAYNLLVGGMLIPLIGAIYWKR 403 Query: 415 ATRQGALMAMVLGLVSWITLEYAAPDGL 442 AT GA+ +M LG ++ L + DGL Sbjct: 404 ATTAGAITSMTLGFLT--VLVFMIKDGL 429 >PA0287 [ER] COG0591 Na+/proline symporter Pae Pseudomonas aeruginosa Length = 461 Score = 103 bits (258), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 124/476 (26%), Positives = 219/476 (46%), Gaps = 57/476 (11%) Query: 35 VLYWVISVGIGLYATRYVKSSKDYAVAGRSLPMSVVTATVFATWFGSEAVLGVSSTFLKD 94 ++Y + +G+G Y R KS D+ VAGR L + T+ A G + +G + Sbjct: 9 LVYALAMLGLGWYGMRKAKSQSDFLVAGRRLGPGLYLGTMAAVVLGGASTIGTVKLGYQF 68 Query: 95 GLGGIVADPFGSSLCLILVGVFFAKKLYRMNLLTIGDYYKKRFGRAAEMLVTICIVISYL 154 GL G+ F L +I++ + F++++ R+ + T+ ++R+ +A+ L+ ++++Y Sbjct: 69 GLSGLWL-VFMLGLGIIVLSLVFSRQIARLRVFTVTQVLEQRY-QASSRLIGGVVMVAYD 126 Query: 155 GWVAAQIK-ALGLVFNVVSGGYIPMHWGMIIGAGSVLIYTLMGGMWAVAITDFLQMIIVV 213 VA A+G V VV G IP ++ G G V++Y+++GGMW++ +TD +Q +I Sbjct: 127 LMVAVTATIAIGSVTEVVFG--IPRIPAILCGGGIVIVYSVIGGMWSLTLTDIIQFVIKT 184 Query: 214 VGMLYIGYEVSGMVGGPGVVIAHAAEAGKFNFWPAAELKDVLWFFAAWITMMLGSIPQQD 273 VG+ + +S G + AG F+ L +L +F + G++ QD Sbjct: 185 VGIFLVLLPLSIDGAGGLARMQEVLPAGFFDLGHIG-LDTILTYFLLY---FFGALIGQD 240 Query: 274 VFQRVMSSKNENVARNASVLGGVIYFFFVFIPIFLAYSATLIDPAMV---SRLLETDSQL 330 ++QRV ++++E V R A + GV + + +A ++ P + + E ++ Sbjct: 241 IWQRVFTARSETVVRYAGLGAGVYCMLYGAACALIGAAARVLLPDLAVPENAYAEITREV 300 Query: 331 VLPTLIMEKMPVFAQVMFFGALLSAIKSCASATLLAPSVTFTENVL-REIRPWKSDREFL 389 + P L + + A LSAI S AS LLA + E++ R +RP + L Sbjct: 301 LAPGL---------RGLVVAAALSAIMSTASGCLLAAATVLQEDIYARFLRPGTTSDIRL 351 Query: 390 FSMRAVVLVFTVGVTTLAF--------------------------GVYWQRATRQGALMA 423 R + L+ V + LA + W+RA+ QGA+ + Sbjct: 352 --SRCITLLMGVAMLVLACLVNDVIAALSIAYNLLVGGLLVPIVGALLWRRASPQGAIAS 409 Query: 424 MVLGLVSWITLEYAAPDGLW--PPQLAGLLFSIGGMVVGSLMPQVLAPVPPMVAVP 477 +V G ++ I A DGL P + GLL S+ V SL + P + A P Sbjct: 410 IVAGCLAVIACM--ARDGLLANSPIVYGLLASLATFVAVSLATR---PAASLRAQP 460 >STM3382 [H] COG4145 Na+/panthothenate symporter Sty Salmonella typhimurium LT2 Length = 483 Score = 91.3 bits (225), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 106/433 (24%), Positives = 189/433 (43%), Gaps = 48/433 (11%) Query: 34 VVLYWVISVGIGLYATRYVKSS---KDYAVAGRSLPMSVVTATVFATWFGSEAVLGVSST 90 +V Y V+ G+ +YA R + +Y + RS+ V+ T+ AT+ + + +G Sbjct: 9 LVAYLVVVFGVSIYAMRKRTAGTFLNEYFLGSRSMGGIVLAMTLTATYISASSFIGGPGA 68 Query: 91 FLKDGLG----GIVADPFGSSLCLILVGVFFAKKLYRMNLLTIGDYYKKRF-GRAAEMLV 145 K GLG ++ P L L ++G FA R N +T+ D R+ R L Sbjct: 69 AYKYGLGWVLLAMIQLP-AVWLSLGILGKKFAILARRYNAVTLNDMLFARYQSRLLVWLA 127 Query: 146 TICIVISYLGWVAAQIKALGLVFNVVSGGYIPMHWGMIIGAGSVLIYTLMGGMWAVAITD 205 ++ ++++++G + Q + +G IP G++I S+ +YT GG A + D Sbjct: 128 SLSLLVAFIGAMTVQFIGGARLLETAAG--IPYETGLLIFGVSIALYTAFGGFRASVLND 185 Query: 206 FLQMIIVVVGMLYIGYEVSGMVGG--PGVVIAHAAEAGKFNFWPAAELKDVLWFFAAWIT 263 LQ ++++VG + + V GG V HA + A ++ + + W+ Sbjct: 186 TLQGLVMLVGTIVLLVGVIHAAGGLSQAVDTLHALDPKLVTPQGADDILSPAFMTSFWVL 245 Query: 264 MMLGSIPQQDVFQRVMSSKNENVARNASVLGGVIYFFFVFIPIFLAYSATLIDPAMVSRL 323 + G I R +S K+ ++G ++ V I +F + A + A++ L Sbjct: 246 VCFGVIGLPHTAVRCISYKDSKAVHRGIIIGTIV----VAILMFGMHLAGALGRAVLPDL 301 Query: 324 LETDSQLVLPTLIMEKMPVFAQVMFFGALLSAIKSCASATLLAPSVTFTENVLREIRPWK 383 D LV+PTL+++ +P FA +F A ++AI S +A LL S T +++ +RP + Sbjct: 302 TVPD--LVIPTLMVKVLPPFAAGIFLAAPMAAIMSTINAQLLQSSATIIKDLYLNLRPDQ 359 Query: 384 SDREFLFSMRAVVLV----------------FTVGVTTLAF-------------GVYWQR 414 E + + + + LAF G+YW+R Sbjct: 360 MQNEIRLKRMSAAITLLLGALLLLAAWKPPEMIIWLNLLAFGGLEAVFLWPLVLGLYWER 419 Query: 415 ATRQGALMAMVLG 427 A GAL AM++G Sbjct: 420 ANAAGALSAMIVG 432 >panF [H] COG4145 Na+/panthothenate symporter Eco Escherichia coli K12 Length = 485 Score = 90.5 bits (223), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 109/434 (25%), Positives = 192/434 (44%), Gaps = 50/434 (11%) Query: 34 VVLYWVISVGIGLYATRYVKSS---KDYAVAGRSLPMSVVTATVFATWFGSEAVLGVSST 90 +V Y V+ GI +YA R + +Y + RS+ V+ T+ AT+ + + +G Sbjct: 11 LVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMGGIVLAMTLTATYISASSFIGGPGA 70 Query: 91 FLKDGLG----GIVADPFGSSLCLILVGVFFAKKLYRMNLLTIGDYYKKRF-GRAAEMLV 145 K GLG ++ P L L ++G FA R N +T+ D R+ R L Sbjct: 71 AYKYGLGWVLLAMIQLP-AVWLSLGILGKKFAILARRYNAVTLNDMLFARYQSRLLVWLA 129 Query: 146 TICIVISYLGWVAAQIKALGLVFNVVSGGYIPMHWGMIIGAGSVLIYTLMGGMWAVAITD 205 ++ ++++++G + Q + +G IP G++I S+ +YT GG A + D Sbjct: 130 SLSLLVAFVGAMTVQFIGGARLLETAAG--IPYETGLLIFGISIALYTAFGGFRASVLND 187 Query: 206 FLQMIIVVVGMLYIGYEVSGMVGGPGVVIAHAAEAGKFNFWPAAELKDVL---WFFAAWI 262 +Q +++++G + + V GG + P D+L + + W+ Sbjct: 188 TMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQTIDPQLVTPQGA-DDILSPAFMTSFWV 246 Query: 263 TMMLGSIPQQDVFQRVMSSKNENVARNASVLGGVIYFFFVFIPIFLAYSATLIDPAMVSR 322 + G I R +S K+ ++G ++ V I +F + A + A++ Sbjct: 247 LVCFGVIGLPHTAVRCISYKDSKAVHRGIIIGTIV----VAILMFGMHLAGALGRAVIPD 302 Query: 323 LLETDSQLVLPTLIMEKMPVFAQVMFFGALLSAIKSCASATLLAPSVTFTENVLREIRPW 382 L D LV+PTL+++ +P FA +F A ++AI S +A LL S T +++ IRP Sbjct: 303 LTVPD--LVIPTLMVKVLPPFAAGIFLAAPMAAIMSTINAQLLQSSATIIKDLYLNIRPD 360 Query: 383 KSDREF-LFSMRAVVLV---------------FTVGVTTLAF-------------GVYWQ 413 + E L M AV+ + + + LAF G+YW+ Sbjct: 361 QMQNETRLKRMSAVITLVLGALLLLAAWKPPEMIIWLNLLAFGGLEAVFLWPLVLGLYWE 420 Query: 414 RATRQGALMAMVLG 427 RA +GAL AM++G Sbjct: 421 RANAKGALSAMIVG 434