BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0150.p (453 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5025 [E] COG2873 O-acetylhomoserine sulfhydrylase Pae Pseudomo... 627 e-179 BH2603 [E] COG2873 O-acetylhomoserine sulfhydrylase Bha Bacillus... 489 e-138 SMc01809 [E] COG2873 O-acetylhomoserine sulfhydrylase Sme Sinorh... 488 e-137 lin0604 [E] COG2873 O-acetylhomoserine sulfhydrylase Lin Listeri... 483 e-136 MA2715 [E] COG2873 O-acetylhomoserine sulfhydrylase Mac Methanos... 480 e-135 >PA5025 [E] COG2873 O-acetylhomoserine sulfhydrylase Pae Pseudomonas aeruginosa Length = 425 Score = 627 bits (1616), Expect = e-179, Method: Compositional matrix adjust. Identities = 298/411 (72%), Positives = 345/411 (83%) Query: 21 KFETLSVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTQD 80 K ETL+VH GY PDPTTRAVAVPIYQT ++AFDDTQHGADLFDLKV GNIY+RIMNPT D Sbjct: 2 KLETLAVHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVPGNIYTRIMNPTND 61 Query: 81 VLEKRLAALEGGIAALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQF 140 VLE+R+AALEGG+ ALA+ASG AA+TYAIQT+AEAGDNIVS + LYGGTYNL AHTLP+ Sbjct: 62 VLEQRVAALEGGVGALAVASGMAAITYAIQTVAEAGDNIVSVAKLYGGTYNLLAHTLPRI 121 Query: 141 GIQTRFADPRQPESFAPLIDERTKAIFIESIGNPLGNVTDIAAVAEIAHRHGVPLIVDNT 200 GIQ RFA + LIDERTKA+F E+IGNP GN+ D+ A+A+ AHRHGVPLIVDNT Sbjct: 122 GIQARFAAHDDVAALEALIDERTKAVFCETIGNPAGNIIDLQALADAAHRHGVPLIVDNT 181 Query: 201 VATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKERFKRLNEPDVS 260 VATP L RPFEHGADIVVHSLTKY+GGHGT++GG +VDSGKF WA +K RF LN PD S Sbjct: 182 VATPVLCRPFEHGADIVVHSLTKYMGGHGTSIGGIVVDSGKFDWAANKSRFPLLNTPDPS 241 Query: 261 YHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLSLRLDRITSNALAV 320 YHGV YTEA G AA+IGR RVVPLRN GAALSPFNAF ILQG+ETL+LR++R NALAV Sbjct: 242 YHGVTYTEAFGPAAFIGRCRVVPLRNMGAALSPFNAFLILQGLETLALRMERHCDNALAV 301 Query: 321 AQYLKRHPKVRWVNYAGLEDHPDHALAQKYFKGRASGVLTFGVANGRDGGARFQDALQLF 380 A+YL++HP+V WV YAGL D+P+HALA++Y GR + +L+FG+ G GARF DAL+L Sbjct: 302 ARYLQQHPQVAWVKYAGLADNPEHALARRYLGGRPAAILSFGIQGGSAAGARFIDALKLV 361 Query: 381 TRLVNIGDAKSLATHPASTTHRQLSPAELEKAGVTEDTVRLSIGIEHIDDL 431 RLVNIGDAKSLA HPASTTHRQL+ EL +AGV++D VRLSIGIEHIDD+ Sbjct: 362 VRLVNIGDAKSLACHPASTTHRQLNAEELARAGVSDDMVRLSIGIEHIDDI 412 >BH2603 [E] COG2873 O-acetylhomoserine sulfhydrylase Bha Bacillus halodurans Length = 430 Score = 489 bits (1259), Expect = e-138, Method: Compositional matrix adjust. Identities = 250/426 (58%), Positives = 316/426 (74%), Gaps = 1/426 (0%) Query: 14 MPDNPQWKFETLSVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 73 M QW+ ET +VH G + DPTT + AVP+YQT ++ F DT H A+LF L GNIY+R Sbjct: 1 MNHENQWQLETKAVHSGQEIDPTTLSRAVPLYQTTSYGFKDTDHAANLFSLSEFGNIYTR 60 Query: 74 IMNPTQDVLEKRLAALEGGIAALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 133 +MNPT DV EKR+AALEGG AALA ASGQAA+TY+I IAEAGD IVSAS+LYGGTYNLF Sbjct: 61 LMNPTTDVFEKRVAALEGGAAALATASGQAAITYSILNIAEAGDEIVSASSLYGGTYNLF 120 Query: 134 AHTLPQFGIQTRFADPRQPESFAPLIDERTKAIFIESIGNPLGNVTDIAAVAEIAHRHGV 193 + TLP+ G+ RF DP PE+F I E+TKAIF ESIGNP G+V DI AVA++AH H + Sbjct: 121 SITLPKLGVNVRFVDPSDPENFKAAITEKTKAIFAESIGNPKGDVLDIEAVAKVAHDHHL 180 Query: 194 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKERFKR 253 PLIVDNT +PYLL+P +HGADIVVHS TK++GGHGT++GG IVD G F WAK +R+ Sbjct: 181 PLIVDNTFPSPYLLQPIKHGADIVVHSATKFIGGHGTSIGGIIVDGGTFDWAK-TDRYPG 239 Query: 254 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLSLRLDRI 313 L PD SYHGVVYT+A+G AYI +ARV LR+ GAA+SPFN+F +LQG+ETL LR++R Sbjct: 240 LTTPDPSYHGVVYTDAVGPIAYIIKARVQLLRDMGAAISPFNSFLLLQGLETLHLRMERH 299 Query: 314 TSNALAVAQYLKRHPKVRWVNYAGLEDHPDHALAQKYFKGRASGVLTFGVANGRDGGARF 373 + NA VA++L++H V V+Y+GL HP + LA+KY +LTF V G + G + Sbjct: 300 SENAYKVAEFLEQHQAVESVSYSGLPSHPSYPLAKKYLPKGQGAILTFEVKGGVEAGKKL 359 Query: 374 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSPAELEKAGVTEDTVRLSIGIEHIDDLLA 433 ++QLF+ L N+GD+KSL HPASTTH+QLS AE AGVT +RLS+G E I D++ Sbjct: 360 IHSVQLFSHLANVGDSKSLIIHPASTTHQQLSEAEQRDAGVTPGMIRLSVGTESIHDIIT 419 Query: 434 DLEQAL 439 DL+QA+ Sbjct: 420 DLKQAI 425 >SMc01809 [E] COG2873 O-acetylhomoserine sulfhydrylase Sme Sinorhizobium meliloti Length = 426 Score = 488 bits (1257), Expect = e-137, Method: Compositional matrix adjust. Identities = 244/419 (58%), Positives = 299/419 (71%), Gaps = 1/419 (0%) Query: 22 FETLSVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTQDV 81 F TL++H G PDPTT A A PIYQT +F F+DT H A LF L+ GNIY+RIMNPTQ V Sbjct: 7 FSTLAIHAGAQPDPTTGARATPIYQTTSFVFNDTDHAAALFGLQQFGNIYTRIMNPTQAV 66 Query: 82 LEKRLAALEGGIAALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQFG 141 LE+R+AALEGG A LA++SG AA TI GDN VSA LYGG+ N F H F Sbjct: 67 LEERIAALEGGTAGLAVSSGHAAQLLVFHTIMRPGDNFVSARQLYGGSANQFGHAFKAFD 126 Query: 142 IQTRFADPRQPESFAPLIDERTKAIFIESIGNPLGNVTDIAAVAEIAHRHGVPLIVDNTV 201 Q R+AD +PESF IDERTKAIFIES+ NP G DIAA+A++A RHG+PLIVDNT+ Sbjct: 127 WQVRWADSAEPESFDAQIDERTKAIFIESLANPGGTFVDIAAIADVARRHGLPLIVDNTM 186 Query: 202 ATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKERFKRLNEPDVSY 261 ATPYL+RP EHGADIVVHSLTK++GGHG ++GG IVD G F W+K ++ L+EP Y Sbjct: 187 ATPYLMRPLEHGADIVVHSLTKFIGGHGNSMGGIIVDGGTFDWSK-SGKYPLLSEPRPEY 245 Query: 262 HGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLSLRLDRITSNALAVA 321 GVV +A G A+ ARV+ LR+ G A+SPFNAF I G+ETL LR+ R NAL VA Sbjct: 246 GGVVLHQAFGNFAFAIAARVLGLRDFGPAISPFNAFLIQTGVETLPLRMQRHCDNALEVA 305 Query: 322 QYLKRHPKVRWVNYAGLEDHPDHALAQKYFKGRASGVLTFGVANGRDGGARFQDALQLFT 381 ++LK H KV WV Y+GLED P+HAL ++Y A V TFG+A G + G RF +AL++F+ Sbjct: 306 KWLKGHEKVSWVRYSGLEDDPNHALQKRYSPKGAGAVFTFGLAGGYEAGKRFVEALEMFS 365 Query: 382 RLVNIGDAKSLATHPASTTHRQLSPAELEKAGVTEDTVRLSIGIEHIDDLLADLEQALG 440 L NIGD +SL HPASTTHRQL+P + AG D +RLS+GIE + D++ADLEQALG Sbjct: 366 HLANIGDTRSLVIHPASTTHRQLTPEQQVAAGAGPDVIRLSVGIEDVADIIADLEQALG 424 >lin0604 [E] COG2873 O-acetylhomoserine sulfhydrylase Lin Listeria innocua Length = 425 Score = 483 bits (1243), Expect = e-136, Method: Compositional matrix adjust. Identities = 241/421 (57%), Positives = 305/421 (72%), Gaps = 2/421 (0%) Query: 19 QWKFETLSVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPT 78 ++KFET+ VHGG+ PD T + AVPIYQT ++ FD +H A LF L+ GNIY+RIMNPT Sbjct: 4 EYKFETIQVHGGHTPDGDTHSRAVPIYQTTSYTFDSPEHAAALFGLQETGNIYTRIMNPT 63 Query: 79 QDVLEKRLAALEGGIAALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLP 138 VLE+RL LEGGI A+A ASG AA+TY+I IA +GD+IV+A+TLYGGT+ LF+HT Sbjct: 64 TAVLEERLTLLEGGIGAVATASGMAAITYSILNIAGSGDHIVAAATLYGGTHTLFSHTFK 123 Query: 139 QFGIQTRFADPRQPESFAPLIDERTKAIFIESIGNPLGNVTDIAAVAEIAHRHGVPLIVD 198 FGI F DP +PE+F + E TKA+FIE+IGNP N+ DI VAEIAH VPLIVD Sbjct: 124 TFGIDVTFVDPNEPENFEKAMKENTKAVFIETIGNPDINIIDIEKVAEIAHASDVPLIVD 183 Query: 199 NTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKERFKRLNEPD 258 NT AT YL RPF+ GADIVV+S TK++GGHG +GGA++DSGKF WA K F +L PD Sbjct: 184 NTFATAYLNRPFDFGADIVVYSATKFIGGHGVAIGGAVIDSGKFNWANGK--FPKLVVPD 241 Query: 259 VSYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLSLRLDRITSNAL 318 SY+G+ YT +G AAYI + RV LR+TGAALSPFNAF ++ G+ETLSLRL++ NA Sbjct: 242 DSYNGLSYTNDVGAAAYITKLRVSLLRDTGAALSPFNAFLLILGLETLSLRLEQHVKNAK 301 Query: 319 AVAQYLKRHPKVRWVNYAGLEDHPDHALAQKYFKGRASGVLTFGVANGRDGGARFQDALQ 378 VA +L HPKV WVNY GL+D+ H LAQKY + TFGV G G + ++++ Sbjct: 302 QVANFLNDHPKVAWVNYPGLKDNKYHDLAQKYLPKGPGSIFTFGVKGGYAAGKKVIESVE 361 Query: 379 LFTRLVNIGDAKSLATHPASTTHRQLSPAELEKAGVTEDTVRLSIGIEHIDDLLADLEQA 438 LF+ L N+GDAKSL HPASTTH+QLS + AGV +++RLSIGIE+ DD++ DL +A Sbjct: 362 LFSHLANVGDAKSLIIHPASTTHQQLSEEQQLTAGVKPESIRLSIGIENADDIIQDLTKA 421 Query: 439 L 439 L Sbjct: 422 L 422 >MA2715 [E] COG2873 O-acetylhomoserine sulfhydrylase Mac Methanosarcina acetivorans str.C2A Length = 441 Score = 480 bits (1235), Expect = e-135, Method: Compositional matrix adjust. Identities = 237/424 (55%), Positives = 297/424 (70%), Gaps = 5/424 (1%) Query: 16 DNPQWKFETLSVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIM 75 +NP TL+VH G PDP T A +VPIYQT A+ FDDT+H ADLF L+ +GNIY+R+M Sbjct: 20 ENPG--ISTLAVHAGAKPDPVTGARSVPIYQTAAYVFDDTEHAADLFGLRKEGNIYTRLM 77 Query: 76 NPTQDVLEKRLAALEGGIAALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLFAH 135 NPT DV EKR+AALEGGI ALA +SG AA+T A+ T GD IVS +YGGTY LF + Sbjct: 78 NPTTDVFEKRIAALEGGIGALATSSGMAAITTALLTFTGPGDEIVSGDKIYGGTYELFNY 137 Query: 136 TLPQFGIQTRFADPRQPESFAPLIDERTKAIFIESIGNPLGNVTDIAAVAEIAHRHGVPL 195 T P+ G +F D PE F I +TKA+++ESIGNP +V D +AEIAH +PL Sbjct: 138 TFPKLGRTVKFVDSSSPEEFKKEITGKTKALYVESIGNPKLDVPDFEKLAEIAHTAEIPL 197 Query: 196 IVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKERFKRLN 255 +VDNTVA P +LRP EHGADIVVHS TKY+GGHGT++GG I+DSG F W+ E+F + Sbjct: 198 VVDNTVA-PVILRPIEHGADIVVHSATKYIGGHGTSIGGVIIDSGNFDWS--SEKFPEIC 254 Query: 256 EPDVSYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLSLRLDRITS 315 +PD YHG+ Y EA G+AA+I +ARV +R+ GA LSPFN+F G+ETL LR+ + Sbjct: 255 DPDPGYHGLKYKEAFGKAAFIAKARVQFMRDIGACLSPFNSFLFTLGLETLPLRVKKHCD 314 Query: 316 NALAVAQYLKRHPKVRWVNYAGLEDHPDHALAQKYFKGRASGVLTFGVANGRDGGARFQD 375 NALAVA++L+ HPKV WV+Y GLE H H LA+KY K ++ FG+ G + +F + Sbjct: 315 NALAVARFLQAHPKVAWVSYPGLESHRSHELAKKYLKSGYGAIIGFGIKGGTEATKKFVE 374 Query: 376 ALQLFTRLVNIGDAKSLATHPASTTHRQLSPAELEKAGVTEDTVRLSIGIEHIDDLLADL 435 +L+LF+ L NIGDAKSL HPASTTH QLS E GVTED +RLSIGIE DL+ DL Sbjct: 375 SLELFSHLANIGDAKSLVIHPASTTHEQLSAEEQRACGVTEDFIRLSIGIEDEKDLIFDL 434 Query: 436 EQAL 439 QAL Sbjct: 435 GQAL 438