BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0073.p (287 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1291 [J] COG0130 Pseudouridine synthase Rso Ralstonia solanac... 349 4e-96 PM0756 [J] COG0130 Pseudouridine synthase Pmu Pasteurella multocida 268 7e-72 PA4742 [J] COG0130 Pseudouridine synthase Pae Pseudomonas aerugi... 265 6e-71 HI1289 [J] COG0130 Pseudouridine synthase Hin Haemophilus influe... 256 6e-68 YPO3494 [J] COG0130 Pseudouridine synthase Ype Yersinia pestis 250 2e-66 >RSc1291 [J] COG0130 Pseudouridine synthase Rso Ralstonia solanacearum Length = 314 Score = 349 bits (896), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 190/287 (66%), Positives = 222/287 (77%), Gaps = 13/287 (4%) Query: 5 NDALIRAKRMLNAPKAGHTGTLDPFATGLLPLCFGEATKFSQDLLEADKTYEALVHLGVR 64 NDALIRAKR+L A KAGHTGTLDP ATGLLPLCFGEATKFSQDLLEADKTYEA+V LG+R Sbjct: 32 NDALIRAKRLLWAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLEADKTYEAVVRLGIR 91 Query: 65 TDTGDTEGQVISERAVEVSVAQIEAALAQFRGPISQVPPMHSALKRDGKPLYEYARAGIT 124 T T D EG+V+SER V V+ Q+ AA+ +F G I QVPPMHSALK+DGKPLYEYARAG T Sbjct: 92 TSTADAEGEVLSERPVAVTPEQLRAAIGRFVGEIDQVPPMHSALKKDGKPLYEYARAGQT 151 Query: 125 LEREARNVTIHALDL----LDYQAPMLRIRVTCSKGTYIRVLGEDIGEVLGCGAHLNALR 180 +ER AR VTI A+D+ LD AP + +RV CSKGTYIR LGED+GE LGCGAHL ALR Sbjct: 152 VERAARRVTIRAIDVLATDLDAAAPTVTLRVCCSKGTYIRTLGEDLGEALGCGAHLVALR 211 Query: 181 RTGVGALTLDGAVTLETFDAAAELEARKALLLPVDGLLRSFPAVELNDELARRFLHGQRL 240 RT VG+LTLDGAVTLE +AA+E + R ALL PVD LL++ P VEL E +RRFLHGQRL Sbjct: 212 RTQVGSLTLDGAVTLEALEAASE-DQRAALLAPVDALLQTLPRVELGAEDSRRFLHGQRL 270 Query: 241 PLGKEGVAVPEQSGRVRVYRS---SDAALLGTGLLQEYAILAPERLV 284 PL +++P + +VRVY + + A+LLG Q +L PERLV Sbjct: 271 PL---QLSLPS-AEQVRVYGARGEAGASLLGVAAWQG-GVLRPERLV 312 >PM0756 [J] COG0130 Pseudouridine synthase Pmu Pasteurella multocida Length = 307 Score = 268 bits (686), Expect = 7e-72, Method: Compositional matrix adjust. Identities = 142/285 (49%), Positives = 181/285 (63%), Gaps = 10/285 (3%) Query: 5 NDALIRAKRMLNAPKAGHTGTLDPFATGLLPLCFGEATKFSQDLLEADKTYEALVHLGVR 64 ND + + KR+ A KAGHTG LDP ATG+LP+C GEATKFSQ LL+ADK Y+ LG R Sbjct: 26 NDIMQKVKRVFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYQVTAKLGER 85 Query: 65 TDTGDTEGQVISERAVEVSVAQIEAALAQFRGPISQVPPMHSALKRDGKPLYEYARAGIT 124 TDT D EGQV+ R V+V V I AAL FRG + QVP M SALK GKPLYEYARAGIT Sbjct: 86 TDTSDAEGQVVETRDVQVDVQDILAALPHFRGNLMQVPTMFSALKHQGKPLYEYARAGIT 145 Query: 125 LEREARNVTIHALDLLDYQAPMLRIRVTCSKGTYIRVLGEDIGEVLGCGAHLNALRRTGV 184 +EREAR +TI L + Y AP L + V CSKGTYIR L +D+GE LGCGAH+ LRRT V Sbjct: 146 VEREARPITIFDLQFIAYDAPYLTLEVHCSKGTYIRTLVDDLGEYLGCGAHVTVLRRTAV 205 Query: 185 GALTLDGAV---TLETFDAAAELEARKALLLPVDGLLRSFPAVELNDELARRFLHGQRLP 241 ++ + TL+ A +L LLP D + + PA+ LN E ++ GQR+ Sbjct: 206 ANYPVEAMMNWDTLQVLAAQQDLALLDQHLLPTDSAVSALPALHLNQEQSKAISFGQRVK 265 Query: 242 LGKEGVAVPEQ-SGRVRVYRSSDAALLGTGLLQEYAILAPERLVS 285 P Q +G+VR++ S LG L+ E+ ++ P+RL++ Sbjct: 266 FDN-----PTQLTGQVRLF-SDTQQFLGVALVDEHNVIRPQRLMT 304 >PA4742 [J] COG0130 Pseudouridine synthase Pae Pseudomonas aeruginosa Length = 304 Score = 265 bits (678), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 144/287 (50%), Positives = 192/287 (66%), Gaps = 13/287 (4%) Query: 5 NDALIRAKRMLNAPKAGHTGTLDPFATGLLPLCFGEATKFSQDLLEADKTYEALVHLGVR 64 N AL + + +LNA KAGHTG+LDP ATG+LPLCFGEATKFSQ LL+ADK YE ++ +G+ Sbjct: 26 NQALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFGEATKFSQYLLDADKGYETVMRMGIT 85 Query: 65 TDTGDTEGQVISERAVEVSVAQIEAALAQFRGPISQVPPMHSALKRDGKPLYEYARAGIT 124 T TGD EG++++ER V V +E AL +FRG I QVPPM+SALK+DG+PLY+ ARAG Sbjct: 86 TTTGDAEGELLAERDVTVGRDDLEQALPRFRGDIEQVPPMYSALKKDGQPLYKLARAGEV 145 Query: 125 LEREARNVTIHALDLLDYQAPMLRIRVTCSKGTYIRVLGEDIGEVLGCGAHLNALRRTGV 184 +EREAR+VTI LDLL ++ P + V+CSKGTY+R L ED+G+VLGCGAH+ ALRRT Sbjct: 146 VEREARSVTITRLDLLSFEPPCATLAVSCSKGTYVRTLVEDLGQVLGCGAHVAALRRTQA 205 Query: 185 GALTLDGAVTLETFD---AAAELEARKALLLPVDGLLRSFPAVELNDELARRFLHGQRLP 241 G L A+TLET + A EA L+P D L +P ++L++ A +LHGQ Sbjct: 206 GPFALAQAITLETLERVHAEGGPEALDQFLMPEDSGLLHWPVLQLSEHSAYYWLHGQ--- 262 Query: 242 LGKEGVAVPE--QSGRVRVYRSSDAALLGTGLLQEYAILAPERLVST 286 V PE + G +RV + +G G + + +AP RL+ + Sbjct: 263 ----PVRAPEAPKFGWLRV-QDHTGRFIGIGEVTDDGRIAPRRLIRS 304 >HI1289 [J] COG0130 Pseudouridine synthase Hin Haemophilus influenzae Length = 312 Score = 256 bits (653), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 138/274 (50%), Positives = 172/274 (62%), Gaps = 8/274 (2%) Query: 5 NDALIRAKRMLNAPKAGHTGTLDPFATGLLPLCFGEATKFSQDLLEADKTYEALVHLGVR 64 ND + + KR+ A KAGHTG LDP ATG+LP+C GEATKFSQ LL+ADK Y LG R Sbjct: 26 NDIMQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYLVTAKLGER 85 Query: 65 TDTGDTEGQVISERAVEVSVAQIEAALAQFRGPISQVPPMHSALKRDGKPLYEYARAGIT 124 TDT D EGQV+ R V + QI AL QFRG I QVP M SALK +GKPLYEYAR GIT Sbjct: 86 TDTSDAEGQVVETREVNLETQQILTALEQFRGDILQVPTMFSALKHNGKPLYEYARQGIT 145 Query: 125 LEREARNVTIHALDLLDYQAPMLRIRVTCSKGTYIRVLGEDIGEVLGCGAHLNALRRTGV 184 +EREAR +TI L+ ++Y AP L + V CSKGTYIR L +D+GEVLGCGAH+ LRRT V Sbjct: 146 VEREARPITIFELNFIEYNAPFLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTMLRRTAV 205 Query: 185 GALTLDGAVTLETFDAAAE---LEARKALLLPVDGLLRSFPAVELNDELARRFLHGQRLP 241 + + + AE L LLLP D + PA+ L+ E ++ GQR+ Sbjct: 206 ADYPVAEMMPINELQLLAESFPLSELDRLLLPTDTAVSKLPALHLDAEQSKAIGFGQRVK 265 Query: 242 LGKEGVAVPEQSGRVRVYRSSDAALLGTGLLQEY 275 E + SG+VR++ S++ LG +EY Sbjct: 266 FANEQ----QLSGQVRLF-SAENLFLGVLNRREY 294 >YPO3494 [J] COG0130 Pseudouridine synthase Ype Yersinia pestis Length = 324 Score = 250 bits (639), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 136/289 (47%), Positives = 181/289 (62%), Gaps = 14/289 (4%) Query: 5 NDALIRAKRMLNAPKAGHTGTLDPFATGLLPLCFGEATKFSQDLLEADKTYEALVHLGVR 64 ND L + KR+ +A +AGHTG LDP ATG+LP+C GEATKFSQ LL++DK Y + LG R Sbjct: 26 NDVLQKVKRLFSANRAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVVARLGQR 85 Query: 65 TDTGDTEGQVISERAVEVSVAQIEAALAQFRGPISQVPPMHSALKRDGKPLYEYARAGIT 124 TDT D EG +ISER V ++ AQI+ AL FRG Q+P M+SALK GKPLYEYAR GI Sbjct: 86 TDTSDAEGALISEREVNLTQAQIDTALESFRGESQQIPSMYSALKHQGKPLYEYARQGIE 145 Query: 125 LEREARNVTIHALDLLDYQAPMLRIRVTCSKGTYIRVLGEDIGEVLGCGAHLNALRRTGV 184 +EREAR++T++ L + ++ L + + CSKGTYIR + +D+GE+LGCGAH++ LRR V Sbjct: 146 VEREARSITVYELLFIRWEGNDLELEIHCSKGTYIRTIIDDLGELLGCGAHVSYLRRLQV 205 Query: 185 GALTLDGAVTLETFDA---AAELEARK------ALLLPVDGLLRSFPAVELNDELARRFL 235 + VTLE A AA+ E R +LLLP+D + +FP V L +A Sbjct: 206 ATYPSERMVTLEQLTAMVEAAQAEGRSPNPELDSLLLPMDSAVLNFPEVNLLPSVAAYVK 265 Query: 236 HGQRLPLGKEGVAVPEQSGRVRVYRSSDAALLGTGLLQEYAILAPERLV 284 GQ P+ G G VR+ + +G G + E +AP+RLV Sbjct: 266 QGQ--PVHVSGAP---SEGMVRITEGKERNFIGIGTIAEDGRVAPKRLV 309