BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0033.p (222 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3718 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LP... 113 4e-25 ZwaaI [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS ... 112 9e-25 ECs4504 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LP... 112 9e-25 rfaI [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS E... 107 2e-23 ZwaaJ [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS ... 106 4e-23 >STM3718 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS Sty Salmonella typhimurium LT2 Length = 337 Score = 113 bits (282), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 65/217 (29%), Positives = 110/217 (50%), Gaps = 6/217 (2%) Query: 2 LKDTAREVLYLDADILCTGSIAGLRALDL-RDDVVAVVSDHLETTVRNRCAALQLP--AD 58 D VLYLDADI C GSI L L+ +++ AVV++ R +L P Sbjct: 119 FSDKTDRVLYLDADIACKGSIQELIDLNFAENEIAAVVAEGELEWWTKRSVSLATPGLVS 178 Query: 59 QYFNSGVLYINVANWIANDITAQVMQAIV--QHGQKLVFPDQDALNIVLAGRARYIEPCW 116 YFN+G + IN+ W A +I+ + ++ + + Q++ DQD LNI L +AR+++ + Sbjct: 179 GYFNAGFILINIPLWTAENISKKAIEMLKDPEVVQRITHLDQDVLNIFLVNKARFVDKKF 238 Query: 117 NFRYNLEFMLNGGQFDTWLPGPAVFLHFTGRVKPWHAWNLHPSRELFSKYLALSPWAGTP 176 N +++L + L + + VF+H+ G KPWH+W +P + F + + SPW+ Sbjct: 239 NTQFSLNYELKDSVINP-VDAETVFVHYIGPTKPWHSWGAYPVSQYFLQAKSNSPWSHCA 297 Query: 177 LDAPRHYKEMRMYAGFLRKKKQWGGWLAWYATYLAHK 213 L P ++R A + +K + + +Y Y K Sbjct: 298 LLNPVTSHQLRYAAKHMFNQKHYTSGINYYIAYFKRK 334 >ZwaaI [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS EcZ Escherichia coli O157:H7 EDL933 Length = 335 Score = 112 bits (279), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 70/214 (32%), Positives = 110/214 (51%), Gaps = 10/214 (4%) Query: 7 REVLYLDADILCTGSIAGLRALDLR---DDVVAVVSDHLETTVRNRCAALQLP--ADQYF 61 ++LYLDADI C GSI L LD + +++ AVV++ +NR + L P A YF Sbjct: 123 EKILYLDADIACKGSIKEL--LDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYF 180 Query: 62 NSGVLYINVANWIANDITAQVMQAIVQHG--QKLVFPDQDALNIVLAGRARYIEPCWNFR 119 N+G L IN+ W N+I+++ ++ + K+ DQD LN++L G+ ++I +N R Sbjct: 181 NAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTR 240 Query: 120 YNLEFMLNGGQFDTWLPGPAVFLHFTGRVKPWHAWNLHPSRELFSKYLALSPWAGTPLDA 179 Y++ + L + D + VF+H+ G KPWH W +P F A SPW+ L Sbjct: 241 YSINYEL-KDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLK 299 Query: 180 PRHYKEMRMYAGFLRKKKQWGGWLAWYATYLAHK 213 P + + R A K+K + + Y Y K Sbjct: 300 PVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEK 333 >ECs4504 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS Ecs Escherichia coli O157:H7 Length = 335 Score = 112 bits (279), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 70/214 (32%), Positives = 110/214 (51%), Gaps = 10/214 (4%) Query: 7 REVLYLDADILCTGSIAGLRALDLR---DDVVAVVSDHLETTVRNRCAALQLP--ADQYF 61 ++LYLDADI C GSI L LD + +++ AVV++ +NR + L P A YF Sbjct: 123 EKILYLDADIACKGSIKEL--LDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYF 180 Query: 62 NSGVLYINVANWIANDITAQVMQAIVQHG--QKLVFPDQDALNIVLAGRARYIEPCWNFR 119 N+G L IN+ W N+I+++ ++ + K+ DQD LN++L G+ ++I +N R Sbjct: 181 NAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTR 240 Query: 120 YNLEFMLNGGQFDTWLPGPAVFLHFTGRVKPWHAWNLHPSRELFSKYLALSPWAGTPLDA 179 Y++ + L + D + VF+H+ G KPWH W +P F A SPW+ L Sbjct: 241 YSINYEL-KDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLK 299 Query: 180 PRHYKEMRMYAGFLRKKKQWGGWLAWYATYLAHK 213 P + + R A K+K + + Y Y K Sbjct: 300 PVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEK 333 >rfaI [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS Eco Escherichia coli K12 Length = 339 Score = 107 bits (268), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 68/216 (31%), Positives = 107/216 (49%), Gaps = 7/216 (3%) Query: 4 DTAREVLYLDADILCTGSIAGLRALDLRDDVVAVVSDHLET---TVRNRCAALQLPADQY 60 + A +VLYLDADI+C G+I L DD VA+V + R + A Y Sbjct: 122 NKAPKVLYLDADIICQGTIEPLINFSFPDDKVAMVVTEGQADWWEKRAHSLGVAGIAKGY 181 Query: 61 FNSGVLYINVANWIANDITAQVMQAIVQHG--QKLVFPDQDALNIVLAGRARYIEPCWNF 118 FNSG L IN A W A ++A+ + + + +K+ PDQD LN++LA + + + +N Sbjct: 182 FNSGFLLINTAQWAAQQVSARAIAMLNEPEIIKKITHPDQDVLNMLLADKLIFADIKYNT 241 Query: 119 RYNLEFMLNGGQFDTWLPGPAVFLHFTGRVKPWHAWNL-HPSRELFSKYLALSPWAGTPL 177 +++L + L F + +F+H+ G KPWH W +P + F + SPW T L Sbjct: 242 QFSLNYQLKES-FINPVTNDTIFIHYIGPTKPWHDWAWDYPVSQAFMEAKNASPWKNTAL 300 Query: 178 DAPRHYKEMRMYAGFLRKKKQWGGWLAWYATYLAHK 213 P + ++R A + KK ++ + Y Y K Sbjct: 301 LKPNNSNQLRYSAKHMLKKHRYLKGFSNYLFYFIEK 336 >ZwaaJ [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS EcZ Escherichia coli O157:H7 EDL933 Length = 337 Score = 106 bits (264), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 61/176 (34%), Positives = 94/176 (53%), Gaps = 10/176 (5%) Query: 9 VLYLDADILCTGSIAGLRALDLRDDVVAVVSDHLETTVRNRCAALQLPAD---QYFNSGV 65 +LYLDADI+C GS+ L AL+ +D+ AVV D ++++ A D YFNSGV Sbjct: 126 LLYLDADIVCKGSLNELIALEFKDEYGAVVID--VDAMQSKSAERLCNEDFNGSYFNSGV 183 Query: 66 LYINVANWIANDITAQVMQAIVQHG--QKLVFPDQDALNIVLAGRARYIEPCWNFRYNL- 122 +YIN+ W+ +T + + +KL +PDQD LN++ A+ + +N Y + Sbjct: 184 MYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQDILNLMFLHHAKILPRKYNCIYTIK 243 Query: 123 -EFMLNGGQFDT-WLPGPAVFLHFTGRVKPWHAWNLHPSRELFSKYLALSPWAGTP 176 EF ++ T ++ VF+H+TG KPWH W + S + F +SPW P Sbjct: 244 SEFEEKNSEYYTRFINDDTVFIHYTGITKPWHDWANYASADYFRNIYNISPWRNIP 299