BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0020.p (259 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr3693 [M] COG0668 Small-conductance mechanosensitive channel N... 222 6e-58 PA4925 [M] COG0668 Small-conductance mechanosensitive channel Pa... 216 4e-56 RSc2662 [M] COG0668 Small-conductance mechanosensitive channel R... 213 4e-55 alr3692 [M] COG0668 Small-conductance mechanosensitive channel N... 206 5e-53 VC0480 [M] COG0668 Small-conductance mechanosensitive channel Vc... 133 4e-31 >alr3693 [M] COG0668 Small-conductance mechanosensitive channel Nos Nostoc sp. PCC 7120 Length = 286 Score = 222 bits (566), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 119/242 (49%), Positives = 164/242 (67%) Query: 16 LVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIHVILRVLLVM 75 L FGL+V+ AI +WI+ + IN K++RR ++ E TL+NY + + VIL+++L++ Sbjct: 14 LTQFGLRVLGAIALWIIAQLLINFGLKLLRRAFRSKHVEPTLINYIVNIVDVILKIVLIV 73 Query: 76 GILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDYITAAGQTG 135 IL GI TTSFAA++ A G+A+G AWSGLL+NFAAG FLV+ RPF VGD I AAG TG Sbjct: 74 AILGFFGIETTSFAALLAAAGIAIGAAWSGLLANFAAGAFLVIFRPFAVGDTIKAAGVTG 133 Query: 136 TVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAYSVDPQQAIA 195 V +IGL TT I T +N++ IIGNNK+F++NI N++ N RR DL Q+ + VD Q AI Sbjct: 134 KVEEIGLFTTTINTSDNVKTIIGNNKIFADNIQNFSANPYRRVDLEAQLHHEVDHQDAIR 193 Query: 196 KLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDVQNAIAEVCLK 255 +L +RI IP+VL PAP + I EFN GT++A+R + ++ QVY D AI E + Sbjct: 194 RLKQRISQIPHVLNNPAPDVEILEFNLLGTVLAVRPYCHHEHYWQVYFDTNKAIRETFGE 253 Query: 256 AA 257 A Sbjct: 254 AG 255 >PA4925 [M] COG0668 Small-conductance mechanosensitive channel Pae Pseudomonas aeruginosa Length = 283 Score = 216 bits (550), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 112/240 (46%), Positives = 159/240 (66%) Query: 12 INLYLVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIHVILRV 71 ++ Y FG+K++AAI W++G I +V++ L ++ + T++ Y S I V L V Sbjct: 21 VSQYAFAFGIKILAAIAFWVIGRWLIGFAVGMVQKSLERQKVDPTVLRYVGSFITVTLNV 80 Query: 72 LLVMGILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDYITAA 131 LLV+GIL GI TTS AA+I AVG+A+G+AWSGLL+N AAG F++VLRPFKVGD+I A Sbjct: 81 LLVVGILGFFGIQTTSLAALIAAVGLAIGMAWSGLLANLAAGGFIIVLRPFKVGDFICAG 140 Query: 132 GQTGTVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAYSVDPQ 191 G TGTV +IGL TT I T +N+ ++GNNK+F +NI N+ N RR +L+ Q++ + D Q Sbjct: 141 GVTGTVKEIGLFTTAINTPDNVLTMVGNNKIFGDNIQNFTHNPFRRVELKAQLSGAADYQ 200 Query: 192 QAIAKLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDVQNAIAE 251 AIA L +R+ AIPNVL P + I EFN G ++A+R + ++ QVY D I + Sbjct: 201 AAIALLKQRVAAIPNVLGEPPVDVEILEFNLVGPVLAVRPYCHNDHYWQVYFDTNKVIKD 260 >RSc2662 [M] COG0668 Small-conductance mechanosensitive channel Rso Ralstonia solanacearum Length = 298 Score = 213 bits (541), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 115/251 (45%), Positives = 164/251 (65%) Query: 7 NFETLINLYLVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIH 66 ++ ++ Y G+K++AAI W++G I+ ++V+ L ++ + T++ Y S I Sbjct: 15 KYQAIVAQYATDVGMKILAAIAFWVIGRWLIHLAVRLVQGSLEHQKVDPTVLRYVGSVIT 74 Query: 67 VILRVLLVMGILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGD 126 V L +LLV+GIL GI TT+FAA+I A GVA+G+AWSGLLSNFAAG FL+VLRPFKVGD Sbjct: 75 VTLNILLVIGILGYFGIQTTTFAALIAAAGVAIGMAWSGLLSNFAAGAFLIVLRPFKVGD 134 Query: 127 YITAAGQTGTVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAY 186 ++T AG TGTV +IGL T + T +N+ ++GNNK+FS+ I NY N RR +L+ Q+A Sbjct: 135 FVTVAGVTGTVKEIGLFATALNTPDNVVTLVGNNKIFSDTIQNYTSNPYRRVELKAQLAG 194 Query: 187 SVDPQQAIAKLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDVQ 246 + D + AIA L E++ AIPNVL P + I EFN G ++A+R H ++ QVY D Sbjct: 195 NADHRAAIALLKEKVGAIPNVLADPPVDVEILEFNLVGPVLAVRPHTHNDHYWQVYFDTN 254 Query: 247 NAIAEVCLKAA 257 I E +A Sbjct: 255 RTIKEALAEAG 265 >alr3692 [M] COG0668 Small-conductance mechanosensitive channel Nos Nostoc sp. PCC 7120 Length = 284 Score = 206 bits (523), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 122/242 (50%), Positives = 162/242 (66%) Query: 16 LVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIHVILRVLLVM 75 L FGLKV+ AI +WIV I K+VRR L + E TL++Y + + V LR++L++ Sbjct: 14 LTQFGLKVLGAIALWIVAQKLIEFALKLVRRGLRTQNIEPTLISYLLNIVSVTLRIVLIV 73 Query: 76 GILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDYITAAGQTG 135 IL GI TTSFAA++ A GVA+G AW GLL+NFAAG FL++ RPFKVGD+ITAAG TG Sbjct: 74 AILGFFGIETTSFAALLAAAGVAIGAAWGGLLANFAAGAFLIIFRPFKVGDFITAAGITG 133 Query: 136 TVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAYSVDPQQAIA 195 TVS+IGL TT ITT +N+ I+ NNK+F++NI N++ N RR DL Q+ + VD QAIA Sbjct: 134 TVSEIGLFTTDITTPDNVLTIVANNKIFADNIQNFSANPFRRVDLLAQLHHDVDHNQAIA 193 Query: 196 KLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDVQNAIAEVCLK 255 L RI IPNV++ PAP + I FN G ++A+R + ++ QVY D AI E + Sbjct: 194 LLKARISQIPNVIENPAPDVEILTFNLAGPVLAVRPYCNNDHYWQVYFDTNKAIRETFGE 253 Query: 256 AA 257 A Sbjct: 254 AG 255 >VC0480 [M] COG0668 Small-conductance mechanosensitive channel Vch Vibrio cholerae Length = 287 Score = 133 bits (335), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 73/236 (30%), Positives = 128/236 (54%) Query: 16 LVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIHVILRVLLVM 75 L+ +G+ VI+AI+I +G + + ++ V VL ++ + +V++ + L +++++ Sbjct: 30 LIQYGVNVISAILILFIGNLVVKGVAGSVANVLKKKEMDKAVVDFIHGLVRYTLFIIVLI 89 Query: 76 GILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDYITAAGQTG 135 L G+ T S A+IGA G+A+G+A G LSNFAAG+ +V RPFK GDY+ G G Sbjct: 90 AALSRIGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAG 149 Query: 136 TVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAYSVDPQQAIA 195 +V I + T++ + +N V++ N+ + I NY+ + TRR D+ ++Y D Q+ Sbjct: 150 SVDSIQIFQTVLKSPDNKMVVVPNSAVIGGAITNYSRHETRRVDMVIGVSYKSDLQKTKR 209 Query: 196 KLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDVQNAIAE 251 L E ++ P +L+ P I + + +R T ++ VY D AI E Sbjct: 210 VLRETLEKDPRILKDPDMTIGVLTLADSSINFVVRPWCKTSDYWAVYFDSMQAIKE 265