BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0046.p (330 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0322 [H] COG0320 Lipoate synthase Rso Ralstonia solanacearum 534 e-152 NMA1378 [H] COG0320 Lipoate synthase NmA Neisseria meningitidis ... 458 e-129 NMB1216 [H] COG0320 Lipoate synthase Nme Neisseria meningitidis ... 457 e-128 PA3996 [H] COG0320 Lipoate synthase Pae Pseudomonas aeruginosa 427 e-119 lipA [H] COG0320 Lipoate synthase Eco Escherichia coli K12 415 e-116 >RSc0322 [H] COG0320 Lipoate synthase Rso Ralstonia solanacearum Length = 333 Score = 534 bits (1376), Expect = e-152, Method: Compositional matrix adjust. Identities = 253/326 (77%), Positives = 281/326 (86%), Gaps = 8/326 (2%) Query: 10 DSSSNATGSARA-----NPLD---KQKGAGKTAWIPIKVVAAEKLKKPDWIRVKAGSPTT 61 DS+S A+ A P D KQK KTA IPIK+V AEKLKKP+WIRVKA + + Sbjct: 3 DSASGASAVANIATPSNEPYDATRKQKSLDKTARIPIKIVPAEKLKKPEWIRVKAATGNS 62 Query: 62 RFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDP 121 RFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDP Sbjct: 63 RFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDP 122 Query: 122 LDPNEPENLAKTIAQLRLSYVVITSVDRDDLRDGGAAHFAACIQRVRELSPETRIEILTP 181 LD NEPENLAKTIAQLRL+YVVITSVDRDDLRDGGA H+ CI R RELSP TRIE+L P Sbjct: 123 LDANEPENLAKTIAQLRLNYVVITSVDRDDLRDGGAQHYVDCISRTRELSPATRIEVLVP 182 Query: 182 DFRGRMDRALEILKMAPPDVMNHNLETAPRLYKEARPGSDYEYSLSLLKRFKDLHPEVPT 241 DFRGR+++AL+IL+ PPDVMNHN+ET PRLYK+ARPG+DY +SL LLK FK +P +PT Sbjct: 183 DFRGRLEKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLKDFKARNPNLPT 242 Query: 242 KSGIMVGLGETDEEVLQVMRDMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEE 301 KSG+MVGLGETDEE+L+VMRDMR H++DMLTIGQYL PSG HLPV RYVHPDTFKM+EE+ Sbjct: 243 KSGLMVGLGETDEEILEVMRDMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFEEK 302 Query: 302 AYKMGFVHAAVGAMVRSSYHADQQAH 327 AY+MGF HAAVGAMVRSSYHADQQAH Sbjct: 303 AYEMGFTHAAVGAMVRSSYHADQQAH 328 >NMA1378 [H] COG0320 Lipoate synthase NmA Neisseria meningitidis Z2491 Length = 327 Score = 458 bits (1179), Expect = e-129, Method: Compositional matrix adjust. Identities = 214/302 (70%), Positives = 257/302 (85%), Gaps = 3/302 (0%) Query: 26 KQKGAGKTAWIPIKVV-AAEKLKKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEEASC 84 K +GA KTA IPIKVV EKLKKP+WIR K P+ +F+EIKDILR + TVCEEASC Sbjct: 14 KLRGADKTARIPIKVVPLQEKLKKPEWIRAKL--PSRKFFEIKDILREQKMHTVCEEASC 71 Query: 85 PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLAKTIAQLRLSYVVI 144 PNIGECF KGTATFMIMGD CTRRCPFCDVGHGRP+ LDP+EP+NLA+++ + L YVVI Sbjct: 72 PNIGECFSKGTATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPKNLAESVKAMNLRYVVI 131 Query: 145 TSVDRDDLRDGGAAHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDVMNH 204 TSVDRDDLRDGGA HFA CI+ +RE SP T+IEIL PDFRGR+D AL+IL PPDVMNH Sbjct: 132 TSVDRDDLRDGGAQHFADCIKAIRETSPNTKIEILVPDFRGRLDIALKILAETPPDVMNH 191 Query: 205 NLETAPRLYKEARPGSDYEYSLSLLKRFKDLHPEVPTKSGIMVGLGETDEEVLQVMRDMR 264 NLET P LY++ARPG++Y++SL LLKR+K++ P +PTKSGIMVGLGETDE+V ++MRDMR Sbjct: 192 NLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETDEDVREIMRDMR 251 Query: 265 AHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYHADQ 324 AHN++M+TIGQYL PS HLPV RYV P+ FK++E+EAY++GF +AA+GAMVRSSYHAD+ Sbjct: 252 AHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADE 311 Query: 325 QA 326 QA Sbjct: 312 QA 313 >NMB1216 [H] COG0320 Lipoate synthase Nme Neisseria meningitidis MC58 Length = 327 Score = 457 bits (1177), Expect = e-128, Method: Compositional matrix adjust. Identities = 214/302 (70%), Positives = 256/302 (84%), Gaps = 3/302 (0%) Query: 26 KQKGAGKTAWIPIKVV-AAEKLKKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEEASC 84 K +GA KTA IPIKVV EKLKKP+WIR K P+ +F+EIKDILR + TVCEEASC Sbjct: 14 KLRGADKTARIPIKVVPLQEKLKKPEWIRAKL--PSRKFFEIKDILREQKMHTVCEEASC 71 Query: 85 PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLAKTIAQLRLSYVVI 144 PNIGECF KGTATFMIMGD CTRRCPFCDVGHGRP+ LDP+EP NLA+++ + L YVVI Sbjct: 72 PNIGECFSKGTATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPRNLAESVKAMNLRYVVI 131 Query: 145 TSVDRDDLRDGGAAHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDVMNH 204 TSVDRDDLRDGGA HFA CI+ +RE SP T+IEIL PDFRGR+D AL+IL PPDVMNH Sbjct: 132 TSVDRDDLRDGGAQHFADCIKAIRETSPNTKIEILVPDFRGRLDIALKILAETPPDVMNH 191 Query: 205 NLETAPRLYKEARPGSDYEYSLSLLKRFKDLHPEVPTKSGIMVGLGETDEEVLQVMRDMR 264 NLET P LY++ARPG++Y++SL LLKR+K++ P +PTKSGIMVGLGETDE+V ++MRDMR Sbjct: 192 NLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETDEDVREIMRDMR 251 Query: 265 AHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYHADQ 324 AHN++M+TIGQYL PS HLPV RYV P+ FK++E+EAY++GF +AA+GAMVRSSYHAD+ Sbjct: 252 AHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADE 311 Query: 325 QA 326 QA Sbjct: 312 QA 313 >PA3996 [H] COG0320 Lipoate synthase Pae Pseudomonas aeruginosa Length = 327 Score = 427 bits (1098), Expect = e-119, Method: Compositional matrix adjust. Identities = 201/304 (66%), Positives = 242/304 (79%), Gaps = 2/304 (0%) Query: 26 KQKGAGKTAWIPIKVVAAEKL-KKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEEASC 84 K +GA K A IP+K++ E+L KKPDWIRV+ + IK +LR + L +VCEEASC Sbjct: 21 KLRGAEKVARIPVKIIPTEELPKKPDWIRVRIPV-SPEVDRIKQLLRKHKLHSVCEEASC 79 Query: 85 PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLAKTIAQLRLSYVVI 144 PN+GECF GTATFMIMGD CTRRCPFCDVGHGRP PLD +EP NLA IA LRL YVVI Sbjct: 80 PNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVDEPTNLAIAIADLRLKYVVI 139 Query: 145 TSVDRDDLRDGGAAHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDVMNH 204 TSVDRDDLRDGGA HFA C++ +R+LSP ++E L PD+RGRMD ALEI PPDV NH Sbjct: 140 TSVDRDDLRDGGAQHFADCLREIRKLSPGIQLETLVPDYRGRMDIALEITANEPPDVFNH 199 Query: 205 NLETAPRLYKEARPGSDYEYSLSLLKRFKDLHPEVPTKSGIMVGLGETDEEVLQVMRDMR 264 NLET PRLY+ +RPGSD+E+SL LL++FK + P VPTKSG+M+GLGETD+EV++VM+ MR Sbjct: 200 NLETVPRLYRSSRPGSDFEWSLDLLQKFKQMVPHVPTKSGLMLGLGETDDEVIEVMQRMR 259 Query: 265 AHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYHADQ 324 H++DMLT+GQYL PS +HLPV+R+VHPDTF + EE KMGF + A G +VRSSYHADQ Sbjct: 260 EHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWFAEEGEKMGFKNVASGPLVRSSYHADQ 319 Query: 325 QAHG 328 QAHG Sbjct: 320 QAHG 323 >lipA [H] COG0320 Lipoate synthase Eco Escherichia coli K12 Length = 321 Score = 415 bits (1067), Expect = e-116, Method: Compositional matrix adjust. Identities = 195/307 (63%), Positives = 233/307 (75%), Gaps = 3/307 (0%) Query: 26 KQKGAGKTAWIPIKVVAAEK---LKKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEEA 82 K + A K A IP+K VA E+ L+KP+W+++K + +TR IK +R N L +VCEEA Sbjct: 12 KYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEA 71 Query: 83 SCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLAKTIAQLRLSYV 142 SCPN+ ECF GTATFMI+G CTRRCPFCDV HGRP D NEP LA+TIA + L YV Sbjct: 72 SCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLAQTIADMALRYV 131 Query: 143 VITSVDRDDLRDGGAAHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDVM 202 VITSVDRDDLRDGGA HFA CI +RE SP+ +IE L PDFRGRMDRAL+IL PPDV Sbjct: 132 VITSVDRDDLRDGGAQHFADCITAIREKSPQIKIETLVPDFRGRMDRALDILTATPPDVF 191 Query: 203 NHNLETAPRLYKEARPGSDYEYSLSLLKRFKDLHPEVPTKSGIMVGLGETDEEVLQVMRD 262 NHNLE PR+Y++ RPG+DY +SL LL+RFK+ HPE+PTKSG+MVGLGET+EE+++VMRD Sbjct: 192 NHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRD 251 Query: 263 MRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYHA 322 +R H V MLT+GQYL PS HLPV+RYV PD F + EA MGF HAA G VRSSYHA Sbjct: 252 LRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHA 311 Query: 323 DQQAHGL 329 D QA G+ Sbjct: 312 DLQAKGM 318