BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0011.p (260 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa1367 [IQR] COG1028 Dehydrogenases with different specificitie... 171 1e-42 CAC3574 [IQR] COG1028 Dehydrogenases with different specificitie... 144 3e-34 VC2021 [IQR] COG1028 Dehydrogenases with different specificities... 140 4e-33 BH2491 [IQR] COG1028 Dehydrogenases with different specificities... 135 8e-32 RSc1052 [IQR] COG1028 Dehydrogenases with different specificitie... 135 9e-32 >SMa1367 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 224 Score = 171 bits (434), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 105/216 (48%), Positives = 135/216 (62%) Query: 45 LVLHARSESPELQAVAQQCRAQGAQCTLALGDLADAHTAAQLRDAALTHYGRLDALVANA 104 L LH + + L+ VA++ A+GA+ LGDL+D L AA +G LD +V+NA Sbjct: 3 LFLHTGTNAEGLRTVAKEVSARGAEVATELGDLSDPTVPGHLVQAARAAFGGLDQIVSNA 62 Query: 105 GFADRKRIGELDDADWSRSLDALLGGFFRLITACREPLQASGQGRVVAVSSFVAHVFRLG 164 G A R G+L DAD + D + FFRL+ A L+ S QGRVVAVSSFVAH F Sbjct: 63 GRAQRSSFGQLTDADLQTAFDMMPMAFFRLVDAALPDLRTSMQGRVVAVSSFVAHGFGTN 122 Query: 165 ESGFPASAAAKAGIEALVKSLAVQVAAAGVTVNAVAPGYVQKSDPAKSAIDPAAWQAALA 224 FPAS AAKA +EAL KSLA Q+A GVTVN VAPG+ +K +A AA ++A A Sbjct: 123 GMHFPASGAAKAALEALAKSLAAQLAPVGVTVNCVAPGFTRKDTGGHAATSSAAMESARA 182 Query: 225 RIPLQRLGQPQEIANVIAFLLSPAASYVTGQVWHVD 260 P RLG+P ++A ++AFLLSP A ++TGQV HVD Sbjct: 183 VTPNGRLGEPIDVAELVAFLLSPGARHITGQVMHVD 218 >CAC3574 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Cac Clostridium acetobutylicum Length = 249 Score = 144 bits (362), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 83/245 (33%), Positives = 137/245 (55%), Gaps = 5/245 (2%) Query: 16 LSVRVILITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTLALG 75 LS +V ++TGA RG+G A+A +AA LV++ RS E Q + ++ G++ Sbjct: 5 LSGKVAVVTGAGRGLGRAIALKLAAEGANLVVNYRSSEAETQKLIKEIEELGSKAVAVKA 64 Query: 76 DLADAHTAAQLRDAALTHYGRLDALVANAGFADRKRIGELDDADWSRSLDALLGGFFRLI 135 D++ A + AL YG +D LV NAG + + + D+ ++ L G F I Sbjct: 65 DISKYDEAETIIKKALDEYGTVDILVNNAGITKDNLLLRMKEEDFDSVINVNLKGAFNCI 124 Query: 136 TACREPLQASGQGRVVAVSSFVAHVFRLGESGFPASAAAKAGIEALVKSLAVQVAAAGVT 195 + G+++ +SS + + G +G AAAKAGI + KS+A ++A+ G+T Sbjct: 125 KHTSRVMLKKKSGKIINISSVIGLI---GNAGQVNYAAAKAGIIGMTKSVAKELASRGIT 181 Query: 196 VNAVAPGYVQKSDPAKSAIDPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASYVTGQ 255 VNAVAPG + KSD + D ++ +A +PL ++G+ +++AN++ FL S +SY+TGQ Sbjct: 182 VNAVAPGII-KSDMTDALTDK-QRESIVAAVPLNKVGEAEDVANLVLFLASDLSSYITGQ 239 Query: 256 VWHVD 260 V +VD Sbjct: 240 VINVD 244 >VC2021 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Vch Vibrio cholerae Length = 248 Score = 140 bits (352), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 89/245 (36%), Positives = 135/245 (55%), Gaps = 9/245 (3%) Query: 16 LSVRVILITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTLALG 75 L +V L+TGA+RGIG A+A +A ++ A SES QA++ G L Sbjct: 7 LEGKVALVTGASRGIGKAIAELLAERGAKVIGTATSESGA-QAISDYLGDNGKGMAL--- 62 Query: 76 DLADAHTAAQLRDAALTHYGRLDALVANAGFADRKRIGELDDADWSRSLDALLGGFFRLI 135 ++ + + + A +G +D LV NAG + + + +WS ++ L FRL Sbjct: 63 NVTNPESIEAVLKAITDEFGGVDILVNNAGITRDNLLMRMKEEEWSDIMETNLTSIFRLS 122 Query: 136 TACREPLQASGQGRVVAVSSFVAHVFRLGESGFPASAAAKAGIEALVKSLAVQVAAAGVT 195 A + QGR++ V S V +G +G AAAKAG+ KS+A +VA+ GVT Sbjct: 123 KAVLRGMMKKRQGRIINVGSVVGT---MGNAGQANYAAAKAGVIGFTKSMAREVASRGVT 179 Query: 196 VNAVAPGYVQKSDPAKSAIDPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASYVTGQ 255 VN VAPG+++ +D K A++ A LA++P RLG P+EIA+ +AFL SP A+Y+TG+ Sbjct: 180 VNTVAPGFIE-TDMTK-ALNDEQRTATLAQVPAGRLGDPREIASAVAFLASPEAAYITGE 237 Query: 256 VWHVD 260 HV+ Sbjct: 238 TLHVN 242 >BH2491 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bha Bacillus halodurans Length = 246 Score = 135 bits (341), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 80/239 (33%), Positives = 124/239 (51%), Gaps = 5/239 (2%) Query: 22 LITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTLALGDLADAH 81 ++TGA+RGIG A A +A +V++ + + V + + G + D+AD+ Sbjct: 8 IVTGASRGIGRATAMELARHGANVVVNYAGNKEKAEKVVAEIKELGVEAIAIQADVADSE 67 Query: 82 TAAQLRDAALTHYGRLDALVANAGFADRKRIGELDDADWSRSLDALLGGFFRLITACREP 141 + + + +G +D LV NAG + + DW +D L G F A P Sbjct: 68 SVQAMVKETIDTFGAVDILVNNAGITRDNLFMRMKEEDWDAVIDTNLKGVFHCSKAVTRP 127 Query: 142 LQASGQGRVVAVSSFVAHVFRLGESGFPASAAAKAGIEALVKSLAVQVAAAGVTVNAVAP 201 + GR++ VSS V + G++ + AAKAG+ L K+LA ++A +TVNAVAP Sbjct: 128 MMKQRFGRIINVSSVVGAIGNAGQANY---VAAKAGVIGLTKTLARELANRNITVNAVAP 184 Query: 202 GYVQKSDPAKSAIDPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASYVTGQVWHVD 260 G+++ + D A L +IPL RLGQP+E+A + FL S ASY+TGQ HV+ Sbjct: 185 GFIETDMTGELPEDVKAQM--LGQIPLARLGQPEEVAKAVRFLASDDASYLTGQTIHVN 241 >RSc1052 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Rso Ralstonia solanacearum Length = 249 Score = 135 bits (340), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 87/248 (35%), Positives = 137/248 (55%), Gaps = 6/248 (2%) Query: 13 SSPLSVRVILITGAARGIGAAVATTVAAPDVALVLHARSESPELQAVAQQCRAQGAQCTL 72 + L+ +V L+TGA+RGIG A+A +A +V A SE+ QA++ G + Sbjct: 2 TQALNNQVALVTGASRGIGRAIALELARQGATVVGTATSEAGA-QAISAYFAEAGVKGAG 60 Query: 73 ALGDLADAHTAAQLRDAALTHYGRLDALVANAGFADRKRIGELDDADWSRSLDALLGGFF 132 + ++ DA + D A+ +G L+ LV NAG + D +W +D L F Sbjct: 61 VVLNVNDAARCEAVIDEAIKTHGGLNILVNNAGITQDNLAMRMKDDEWMAVIDTNLSAVF 120 Query: 133 RLITACREPLQASGQGRVVAVSSFVAHVFRLGESGFPASAAAKAGIEALVKSLAVQVAAA 192 RL A P+ + GR++ ++S V G++ + AAAKAG+E + +++A ++ + Sbjct: 121 RLSRAVLRPMMKARGGRIINITSVVGSAGNPGQANY---AAAKAGVEGMARAMAKEIGSR 177 Query: 193 GVTVNAVAPGYVQKSDPAKSAIDPAAWQAALARIPLQRLGQPQEIANVIAFLLSPAASYV 252 +TVN+VAPG++ +D K D A A+IPL RLG P++IAN +AFL SPAA Y+ Sbjct: 178 NITVNSVAPGFID-TDMTKVLSDEQ-HTALKAQIPLGRLGMPEDIANAVAFLASPAAGYI 235 Query: 253 TGQVWHVD 260 TG HV+ Sbjct: 236 TGATLHVN 243