BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0004.p (401 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4108 [T] COG2206 HD-GYP domain Pae Pseudomonas aeruginosa 386 e-107 RSc2515 [T] COG2206 HD-GYP domain Rso Ralstonia solanacearum 369 e-102 RSc0592 [T] COG2206 HD-GYP domain Rso Ralstonia solanacearum 320 3e-87 AGc4164 [T] COG2206 HD-GYP domain Atu Agrobacterium tumefaciens ... 179 9e-45 CAC0277 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum 172 9e-43 >PA4108 [T] COG2206 HD-GYP domain Pae Pseudomonas aeruginosa Length = 414 Score = 386 bits (991), Expect = e-107, Method: Compositional matrix adjust. Identities = 193/399 (48%), Positives = 263/399 (65%), Gaps = 24/399 (6%) Query: 1 MYVHSIPGGWINHPFW-RKSFKIETLEDLQTLRECPVEEVTIDTAKGRDIAPEHDIDDET 59 M+V S+ G W++HPFW R F +++ DLQ LRE V+EV ID +KG D+ E + Sbjct: 14 MFVQSLCGSWLDHPFWKRGGFLLDSQADLQRLRESAVKEVWIDASKGLDLPEEAAVSAAA 73 Query: 60 DAAEQDDAAP-PAEIAIAHQGASATVAVRKPATRTETAVERERAARIISSSKSTVLNMFS 118 A P PA +A+ E AA + S +K+ V++MF Sbjct: 74 VLPVTMPAGPSPARVALEE--------------------EIRHAALLCSRAKAAVVSMFR 113 Query: 119 EARMGKTVDVKDAAELVTEITSSVSRNADALISLARLKTTDDYTYMHSVAVCAMMISLAN 178 +ARMG+ +D A++LV EI++SV R+ +AL+SL RLKT+D+YTYMHSVAVCA+MI+LA Sbjct: 114 DARMGQAIDTAHASDLVDEISASVLRHPNALLSLVRLKTSDEYTYMHSVAVCALMIALAR 173 Query: 179 QLGMTQEQIKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRSHTVQGHSILKQIE 238 QL + +++AG+AGLLHD+GKMAVP ILNKP KLT+ EF VR H G +L Sbjct: 174 QLELPDPLVREAGLAGLLHDIGKMAVPDPILNKPGKLTDPEFGLVRRHPQNGARMLLDCR 233 Query: 239 GIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPA 298 + + +DV LHHHE+IDG+GYPF L + IS++A+MGAVCDVYDAITS+RPYK GW+ A Sbjct: 234 QVSALVVDVCLHHHERIDGTGYPFGLAQEQISLLARMGAVCDVYDAITSDRPYKKGWNAA 293 Query: 299 RSIRHMAASAGHFDPITMEAFVKAIGIYPTGSCVILQSGRLGIVVDQRPEHLLTPRIKLF 358 +IR MA GHFDP AFVKA+GIYP G+ V L+SGRLG+V++Q LLTPR+K+F Sbjct: 294 EAIRRMAEWNGHFDPQVFRAFVKAVGIYPVGALVRLESGRLGVVLEQHGRSLLTPRVKVF 353 Query: 359 YSTTSRMPLKPEIIDLARGG--DKIVAYADPAKWGIRSL 395 +S S++P+ +++DL R G D+IV + W R+L Sbjct: 354 FSARSKVPIPQQVVDLGRAGQTDRIVGFEPAEAWNFRNL 392 >RSc2515 [T] COG2206 HD-GYP domain Rso Ralstonia solanacearum Length = 402 Score = 369 bits (946), Expect = e-102, Method: Compositional matrix adjust. Identities = 186/396 (46%), Positives = 261/396 (65%), Gaps = 16/396 (4%) Query: 1 MYVHSIPGGWINHPFWRKSFKIETLEDLQTLRECPVEEVTIDTAKGRDIAPEHDIDDETD 60 MY+ W++HPFWR F++E LQ LR + EV IDT++G+D+A Sbjct: 14 MYLEEFVDSWLDHPFWRARFRVEDTAQLQRLRASGIREVWIDTSRGKDVAAG--AAAPAA 71 Query: 61 AAEQDDAAPPAEIAIAHQGASATVAVRKPATRTETAVERERAARIISSSKSTVLNMFSEA 120 A P E AIA +PAT A E RAARII + V MF+EA Sbjct: 72 ADMPAPPQPQLETAIAP----------RPAT---LADEMRRAARIIDKAGIAVQAMFAEA 118 Query: 121 RMGKTVDVKDAAELVTEITSSVSRNADALISLARLKTTDDYTYMHSVAVCAMMISLANQL 180 RMG+T++ +DA LV I+ SV R+ AL+SLARLKT D YT++HSVAVCA+M++L QL Sbjct: 119 RMGRTIEARDAQPLVEAISGSVDRHPGALVSLARLKTKDRYTFLHSVAVCALMVALGRQL 178 Query: 181 GMTQEQIKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRSHTVQGHSILKQIEGI 240 G++ ++++ AG+AGLLHD+GK+A+P ++LNKP LT +EF V H G+ IL + Sbjct: 179 GLSDDEVRDAGLAGLLHDIGKIAIPPEVLNKPGALTTDEFRHVIGHPKAGYDILVGDPSM 238 Query: 241 GEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARS 300 ++ALDV LHHHE++DG+GYP L ++IS+ A+MGA+CDVYDA+TS+RPYK W+ A + Sbjct: 239 RDIALDVCLHHHERMDGTGYPHKLAGENISLFARMGAICDVYDAVTSDRPYKKAWEAAYA 298 Query: 301 IRHMAASAG-HFDPITMEAFVKAIGIYPTGSCVILQSGRLGIVVDQRPEHLLTPRIKLFY 359 I+ M G HFDP+ +AFVK++GIYPTGS V L+SGRL +V++ LL+P +K+F+ Sbjct: 299 IQRMVEWRGNHFDPMVFQAFVKSVGIYPTGSLVRLRSGRLAVVMEHASGALLSPTVKVFF 358 Query: 360 STTSRMPLKPEIIDLARGGDKIVAYADPAKWGIRSL 395 S +S + PE+IDLA+ GD+IV+ +P WG+ L Sbjct: 359 SISSGTRITPEVIDLAQTGDQIVSREEPGDWGLTDL 394 >RSc0592 [T] COG2206 HD-GYP domain Rso Ralstonia solanacearum Length = 403 Score = 320 bits (821), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 155/393 (39%), Positives = 236/393 (60%), Gaps = 14/393 (3%) Query: 1 MYVHSIPGGWINHPFWRKSFKIETLEDLQTLRECPVEEVTIDTAKGRDIAPEHDIDDETD 60 M+V + G W+ HPFWR F++ + + + + E+ ID +G D+ Sbjct: 13 MFVVKLGGSWLKHPFWRSQFQLSSQAQVDDICRAGITEIWIDPERGEDVLAP-------- 64 Query: 61 AAEQDDAAPPAEIAIAHQGASATVAVRKPATRTETAVERERAARIISSSKSTVLNMFSEA 120 P A T+A P T T E + A +++ S K+T+ +FSEA Sbjct: 65 -----ALMPAAAQPAPEPLTRETLAALPPVTPTSLKEEWKHAQQLVQSGKATLGQLFSEA 119 Query: 121 RMGKTVDVKDAAELVTEITSSVSRNADALISLARLKTTDDYTYMHSVAVCAMMISLANQL 180 RMG+ + A LV +++S++RN+ ALI+LARLK DDYTY+HS AVCA+M++LA L Sbjct: 120 RMGRALQTDKALLLVDNVSNSLARNSYALIALARLKNKDDYTYLHSFAVCALMVALARTL 179 Query: 181 GMTQEQIKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRSHTVQGHSILKQIEGI 240 G+++++I++ G+ GL+HD+GK A+P +L+K LT+EE A +++H V GH +L Sbjct: 180 GLSEDEIRECGLGGLVHDIGKSAMPRTLLDKSTALTKEELALLQTHAVGGHHLLIGTGQF 239 Query: 241 GEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARS 300 E+ +V LHHHE+IDGSGYP K D IS+ AKMGA+CDVYD +TS+ PY W PA++ Sbjct: 240 SEIPREVCLHHHERIDGSGYPDGQKGDEISLWAKMGAICDVYDTLTSSSPYHHAWSPAQA 299 Query: 301 IRHMAA-SAGHFDPITMEAFVKAIGIYPTGSCVILQSGRLGIVVDQRPEHLLTPRIKLFY 359 +++M A + FD +AF +++GIYP G+ V L++ RLG+VV Q L P + +FY Sbjct: 300 LKYMMARTDSQFDRTVFQAFTRSVGIYPVGTLVKLRTNRLGVVVHQNESSALRPDVVVFY 359 Query: 360 STTSRMPLKPEIIDLARGGDKIVAYADPAKWGI 392 S ++ ++PE I L R D I+ D + WG+ Sbjct: 360 SGNTKTRVRPERISLGRSDDAIITVEDASTWGL 392 >AGc4164 [T] COG2206 HD-GYP domain Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 315 Score = 179 bits (455), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 106/325 (32%), Positives = 163/325 (50%), Gaps = 32/325 (9%) Query: 1 MYVHSIPGGWINHPFWRK-SFKIETLEDLQTLRECPVEEVTIDTAKGRDIAPEHDIDDET 59 M++ ++ G + NH +K F ++ E +Q L++ E + I+TAKG D+ Sbjct: 14 MFIEAVEGTFSNHKITQKYRFLLQREEVVQQLKKSGAESIVINTAKGSDVG--------- 64 Query: 60 DAAEQDDAAPPAEIAIAHQG-ASATVAVRKPATRTETAVERERAARIISSSKSTVLNMFS 118 A QG SA V+ P+ TAV + + S+ + FS Sbjct: 65 ----------------ASQGPGSAGVSQTDPSPSMVTAVTQA-----VRSAADVIAETFS 103 Query: 119 EARMGKTVDVKDAAELVTEITSSVSRNADALISLARLKTTDDYTYMHSVAVCAMMISLAN 178 A G V + +I+ ++ N I + RLK+ D+ T++HSV+V +MI N Sbjct: 104 VAEGGGCVSFQGMTAAAGKISDAIQANPAVFIGVTRLKSKDETTFVHSVSVSGLMILFGN 163 Query: 179 QLGMTQEQIKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRSHTVQGHSILKQIE 238 LG+ +E + G++GLLHD+GK+ +P ILNK L+ EE + H G IL + Sbjct: 164 YLGLNKETVNLLGVSGLLHDIGKVEIPSSILNKTEGLSIEEREIINLHPAFGRDILSRDG 223 Query: 239 GIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPA 298 + ++ LDV +HHE+IDG GYP IS+ A++ A+CDVYDA+TS RPYK W Sbjct: 224 QMPDMVLDVCFNHHERIDGGGYPSGRAGGEISLHARIAAICDVYDAVTSVRPYKKPWTAN 283 Query: 299 RSIRHMAASAGHFDPITMEAFVKAI 323 ++ M GHFD ++ F I Sbjct: 284 DALTWMLRRDGHFDIQLLKKFALCI 308 >CAC0277 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum Length = 372 Score = 172 bits (437), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 92/247 (37%), Positives = 142/247 (57%), Gaps = 5/247 (2%) Query: 115 NMFSEARMGKTVDVKDAAELVTEITSSVSRNADALISLARLKTTDDYTYMHSVAVCAMMI 174 N++ E+ D K + TEI + + +I L +K+ DDYTY HSV V + + Sbjct: 97 NLYKESEQ----DFKSIYYIATEIIEEILSQKNVMIGLVDIKSFDDYTYQHSVNVAVISL 152 Query: 175 SLANQLGMTQEQIKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRSHTVQGHSIL 234 + +L + ++ + LLHDVGKM +P +ILNK +LTEEEF ++ HTV+G+ L Sbjct: 153 IIGIKLKFHKYELFDLCIGALLHDVGKMFIPSEILNKKGELTEEEFKIMKEHTVRGYDYL 212 Query: 235 KQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAG 294 K + I A +SL HHE+ G GYP + ISI++++ AV DVYDA+TS+R Y+ Sbjct: 213 KGLYEISVPARIISLQHHERAGGQGYPEGRSGERISILSRIVAVADVYDAMTSDRVYRRA 272 Query: 295 WDPARSIRHMAASAG-HFDPITMEAFVKAIGIYPTGSCVILQSGRLGIVVDQRPEHLLTP 353 P ++ ++ A++G FD ++AF K++ YP GS V L + +G+V + L P Sbjct: 273 IAPNEALEYIMANSGSKFDYKVVQAFSKSVIPYPEGSIVRLSNNEIGVVTKIHEDFPLRP 332 Query: 354 RIKLFYS 360 IK+ YS Sbjct: 333 EIKIIYS 339