BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0026.p (429 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ECs5316 [GEPR] COG0477 Permeases of the major facilitator superf... 415 e-115 ZyjiZ [GEPR] COG0477 Permeases of the major facilitator superfam... 414 e-115 yjiZ [GEPR] COG0477 Permeases of the major facilitator superfami... 412 e-115 yhaU [GEPR] COG0477 Permeases of the major facilitator superfami... 223 1e-57 ZyhaU [GEPR] COG0477 Permeases of the major facilitator superfam... 223 1e-57 >ECs5316 [GEPR] COG0477 Permeases of the major facilitator superfamily Ecs Escherichia coli O157:H7 Length = 453 Score = 415 bits (1066), Expect = e-115, Method: Compositional matrix adjust. Identities = 204/423 (48%), Positives = 285/423 (67%), Gaps = 8/423 (1%) Query: 6 EKSPRLKRIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAY 65 ++S R+KRIQ A+ L A ++NYLDRS+LS+AN ++ +EL LSA+++G LLS FSLAY Sbjct: 31 QRSTRIKRIQTTAMLLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAY 90 Query: 66 AFSQLPIGAMLDKFGARVMLGAGMFVWSLAQLAGGFVQTLQQFLVARVFLGLGEAPQFPA 125 +QLP G +LD+ G R+MLG GMF WSL Q G V + QF++ R+ +G+GEAP P Sbjct: 91 GIAQLPCGPLLDRKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPC 150 Query: 126 GAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFIIMGLLGIAVS 185 G KV+++WF ++ERG P G F A+STIG A++PPIL +ML GWR MFI +G+LGI ++ Sbjct: 151 GVKVINDWFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLA 210 Query: 186 VGWYLFYRNRNEVQLTPSEVLYL---ADDHDQDPNANAKLSWAEWKSLFTYRTTWGMLFG 242 +GWY+ YRNR V+LT E YL + + +DP LS+AEW+SLF RT WGM+ G Sbjct: 211 IGWYMLYRNREHVELTAVEQAYLNAGSVNARRDP-----LSFAEWRSLFRNRTMWGMMLG 265 Query: 243 FMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGM 302 F G+ Y WLYL WLP YL+ +L + TG + IP++FG GML +GY+ D L+K GM Sbjct: 266 FSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGM 325 Query: 303 PAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIP 362 I+SRK I AG+ + AFT+ ++ MA++ I +A+ ++ A W L+ VA+ Sbjct: 326 APIKSRKICIIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVA 385 Query: 363 RRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVVDK 422 R+ AS+GS+QNF ++ S AP+ITG +VD THSF AL+I V A AL Y+F+V + Sbjct: 386 SRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQ 445 Query: 423 PIE 425 PI Sbjct: 446 PIN 448 >ZyjiZ [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 453 Score = 414 bits (1064), Expect = e-115, Method: Compositional matrix adjust. Identities = 203/423 (47%), Positives = 285/423 (67%), Gaps = 8/423 (1%) Query: 6 EKSPRLKRIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAY 65 ++S R+KRIQ A+ L A ++NYLDRS+LS+AN ++ +EL LSA+++G LLS FSLAY Sbjct: 31 QRSTRIKRIQTTAMLLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAY 90 Query: 66 AFSQLPIGAMLDKFGARVMLGAGMFVWSLAQLAGGFVQTLQQFLVARVFLGLGEAPQFPA 125 +QLP G +LD+ G R+MLG GMF WSL Q G V + QF++ R+ +G+GEAP P Sbjct: 91 GIAQLPCGPLLDRKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPC 150 Query: 126 GAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFIIMGLLGIAVS 185 G KV+++WF ++ERG P G F A+STIG A++PPIL +ML GWR MFI +G+LGI ++ Sbjct: 151 GVKVINDWFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLA 210 Query: 186 VGWYLFYRNRNEVQLTPSEVLYL---ADDHDQDPNANAKLSWAEWKSLFTYRTTWGMLFG 242 +GWY+ YRNR V+LT E YL + + +DP LS+AEW+SLF RT WGM+ G Sbjct: 211 IGWYMLYRNREHVELTAVEQAYLNAGSVNARRDP-----LSFAEWRSLFRNRTMWGMMLG 265 Query: 243 FMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGM 302 F G+ Y WLYL WLP YL+ +L + TG + IP++FG GML +GY+ D L+K GM Sbjct: 266 FSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGM 325 Query: 303 PAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIP 362 I+SRK I AG+ + AFT+ ++ MA++ I +A+ ++ A W L+ VA+ Sbjct: 326 APIKSRKICIIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVA 385 Query: 363 RRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVVDK 422 R+ AS+GS+QNF ++ S AP+ITG +VD THSF AL+I V A AL Y+F+V + Sbjct: 386 SRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQ 445 Query: 423 PIE 425 P+ Sbjct: 446 PLN 448 >yjiZ [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 453 Score = 412 bits (1059), Expect = e-115, Method: Compositional matrix adjust. Identities = 203/423 (47%), Positives = 283/423 (66%), Gaps = 8/423 (1%) Query: 6 EKSPRLKRIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAY 65 ++S R+KRIQ A+ L A ++NYLDRS+LS+AN ++ +EL LSA+++G LLS FSLAY Sbjct: 31 QRSTRIKRIQTTAMLLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAY 90 Query: 66 AFSQLPIGAMLDKFGARVMLGAGMFVWSLAQLAGGFVQTLQQFLVARVFLGLGEAPQFPA 125 +QLP G +LD+ G R+MLG GMF WSL Q G V QF++ R+ +G+GEAP P Sbjct: 91 GIAQLPCGPLLDRKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPC 150 Query: 126 GAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFIIMGLLGIAVS 185 G KV+++WF ++ERG P G F A+STIG A++PPIL +ML GWR MFI +G+LGI ++ Sbjct: 151 GVKVINDWFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLA 210 Query: 186 VGWYLFYRNRNEVQLTPSEVLYL---ADDHDQDPNANAKLSWAEWKSLFTYRTTWGMLFG 242 +GWY+ YRNR V+LT E YL + + +DP LS+AEW+SLF RT WGM+ G Sbjct: 211 IGWYMLYRNREHVELTAVEQAYLNAGSVNARRDP-----LSFAEWRSLFRNRTMWGMMLG 265 Query: 243 FMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGM 302 F G+ Y WLYL WLP YL+ +L + TG + IP++FG GML +GY+ D L+K GM Sbjct: 266 FSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGM 325 Query: 303 PAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIP 362 I+SRK I AG+ + AFT+ ++ A++ I +A+ ++ A W L+ VA+ Sbjct: 326 APIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVA 385 Query: 363 RRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVVDK 422 R+ AS+GS+QNF ++ S AP+ITG +VD THSF AL+I V A AL Y+F+V + Sbjct: 386 SRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQ 445 Query: 423 PIE 425 PI Sbjct: 446 PIN 448 >yhaU [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 444 Score = 223 bits (567), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 145/436 (33%), Positives = 227/436 (52%), Gaps = 25/436 (5%) Query: 3 VQKEKSPRLKRIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFS 62 V ++K R ++ + F++ A VNY DR+TLSIA V++EL LSA MG + SAF Sbjct: 6 VDEKKKGVHTRYLILLIIFIVTA--VNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFG 63 Query: 63 LAYAFSQLPIGAMLDKFGARVMLGAGMFVWSLAQLAGGFVQTLQ------QFLVARVFLG 116 AY Q+P G +LDKFG++ + +F WSL GFV R LG Sbjct: 64 WAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLG 123 Query: 117 LGEAPQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFII 176 EAP FPA A++V+ WF +ERG + IF ++ AL P+L L ++GW +F + Sbjct: 124 FSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTV 183 Query: 177 MGLLGIAVSVGWY-LFYRNRNEVQLTPSEVLYLAD-------DHDQDPNANA---KLSWA 225 MG++G ++ W L + + +++ E+ ++++ DH + +A A KL + Sbjct: 184 MGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYI 243 Query: 226 EWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTL 285 K L + R G+ FG + + W +LTW P YL E+ +SI + G V IP + G Sbjct: 244 --KQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFA 301 Query: 286 GMLSSGYIADGLMKRGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAF 345 G + G +D L+KRG+ +RK PI G++ A I YT +TT+ ++ +A+AF Sbjct: 302 GGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLAST-IILCNYTNNTTLVVML--MALAF 358 Query: 346 VNMASGG-AWALVSVAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLI 404 G W ++S P+ +V G + N G + + P++ G +V + HSF AL+ Sbjct: 359 FGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVF 418 Query: 405 SAAVAFAAALVYMFVV 420 A A + Y+FVV Sbjct: 419 VGCSALMAMVCYLFVV 434 >ZyhaU [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 444 Score = 223 bits (567), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 145/436 (33%), Positives = 227/436 (52%), Gaps = 25/436 (5%) Query: 3 VQKEKSPRLKRIQLVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFS 62 V ++K R ++ + F++ A VNY DR+TLSIA V++EL LSA MG + SAF Sbjct: 6 VDEKKKGVHTRYLILLIIFIVTA--VNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFG 63 Query: 63 LAYAFSQLPIGAMLDKFGARVMLGAGMFVWSLAQLAGGFVQTLQ------QFLVARVFLG 116 AY Q+P G +LDKFG++ + +F WSL GFV R LG Sbjct: 64 WAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLG 123 Query: 117 LGEAPQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFII 176 EAP FPA A++V+ WF +ERG + IF ++ AL P+L L ++GW +F + Sbjct: 124 FSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTV 183 Query: 177 MGLLGIAVSVGWY-LFYRNRNEVQLTPSEVLYLAD-------DHDQDPNANA---KLSWA 225 MG++G ++ W L + + +++ E+ ++++ DH + +A A KL + Sbjct: 184 MGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYI 243 Query: 226 EWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTL 285 K L + R G+ FG + + W +LTW P YL E+ +SI + G V IP + G Sbjct: 244 --KQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFA 301 Query: 286 GMLSSGYIADGLMKRGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAF 345 G + G +D L+KRG+ +RK PI G++ A I YT +TT+ ++ +A+AF Sbjct: 302 GGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLAST-IILCNYTNNTTLVVML--MALAF 358 Query: 346 VNMASGG-AWALVSVAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLI 404 G W ++S P+ +V G + N G + + P++ G +V + HSF AL+ Sbjct: 359 FGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVF 418 Query: 405 SAAVAFAAALVYMFVV 420 A A + Y+FVV Sbjct: 419 VGCSALMAMVCYLFVV 434