BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0174.p (430 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1149 [G] COG2706 3-carboxymuconate cyclase Ype Yersinia pestis 167 3e-41 ZybhE [G] COG2706 3-carboxymuconate cyclase EcZ Escherichia coli... 163 6e-40 ybhE [G] COG2706 3-carboxymuconate cyclase Eco Escherichia coli K12 163 8e-40 ECs0795 [G] COG2706 3-carboxymuconate cyclase Ecs Escherichia co... 163 8e-40 STM0785 [G] COG2706 3-carboxymuconate cyclase Sty Salmonella typ... 155 1e-37 >YPO1149 [G] COG2706 3-carboxymuconate cyclase Ype Yersinia pestis Length = 334 Score = 167 bits (424), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 100/281 (35%), Positives = 152/281 (54%), Gaps = 10/281 (3%) Query: 34 AYVSNADSQDIYVLRLNGDGSVNLIDKVDTGSTVMPLALSPDHKFLYAALRGQPYSVVSY 93 YV++ DSQ I+V +L+ G + L+ VD V P+A+SP+ + LY +R + +VSY Sbjct: 5 VYVASPDSQQIHVWQLDSAGELTLLQTVDVPGQVQPMAISPNQRHLYVGVRPD-FGIVSY 63 Query: 94 AIDPASGKLKALSKVPLADNMANIATDRSGRYLLAASYSGNRISVNAIGTDGIVQAP--P 151 I G L A PL + +I TDR GR+L +ASYS N +S++ I T G+VQAP Sbjct: 64 HI-ADDGTLTAAGMAPLPGSPTHIDTDRQGRFLFSASYSFNCVSISPIDTHGVVQAPIQQ 122 Query: 152 LAVIPTGKNAHSVQVSPDNAFVFASNLGSDVILQYRFDPATGTVTPNSPPSVATKAGAGP 211 L +P HS + P N + L D + + A G +TP++ + AGAGP Sbjct: 123 LDDLPA---PHSANIDPTNQILLVPCLKEDKVRLFDLS-AEGQLTPHAQADITVAAGAGP 178 Query: 212 RHFVFSPDQRFVYSTNELDATVNTYAYDRQSGTLTLLASDSALPEGFKTSEQLAAADLHL 271 RH F P+ + Y NEL+++V+ Y L+ S A+P F + AAD+H+ Sbjct: 179 RHMAFHPNHQVAYCVNELNSSVDVYQISNNGQEYHLVQSLDAMPADFTGTRW--AADIHI 236 Query: 272 TPDGRFLYATERASNTLSAYRVDPAGGKLTRILNIPTETQP 312 TP+GR+LY ++R +N L + V G ++ + + TE QP Sbjct: 237 TPNGRYLYISDRTANLLGIFTVSEDGRVISLVGHHLTEAQP 277 >ZybhE [G] COG2706 3-carboxymuconate cyclase EcZ Escherichia coli O157:H7 EDL933 Length = 331 Score = 163 bits (413), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 92/282 (32%), Positives = 153/282 (54%), Gaps = 12/282 (4%) Query: 34 AYVSNADSQDIYVLRLNGDGSVNLIDKVDTGSTVMPLALSPDHKFLYAALRGQPYSVVSY 93 Y+++ +SQ I+V LN +G++ L VD V P+ +SPD ++LY +R + + V++Y Sbjct: 5 VYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVSPDKRYLYVGVRPE-FRVLAY 63 Query: 94 AIDPASGKLKALSKVPLADNMANIATDRSGRYLLAASYSGNRISVNAIGTDGIVQAPPLA 153 I P G L ++ L + +I+TD G+++ SY+ +SV + DG+ P+ Sbjct: 64 RIAPDDGALTFAAEXALPGSPTHISTDHQGQFVFVGSYNAGNVSVTRL-EDGL----PVG 118 Query: 154 VIPTGKN---AHSVQVSPDNAFVFASNLGSDVILQYRFDPATGTVTPNSPPSVATKAGAG 210 V+ + HS +SPDN ++ L D I + G + P V T GAG Sbjct: 119 VVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSD-DGHLVAQDPAEVTTVEGAG 177 Query: 211 PRHFVFSPDQRFVYSTNELDATVNTYAYDRQSGTLTLLASDSALPEGFKTSEQLAAADLH 270 PRH VF P++++ Y NEL+++V+ + G + + + +PE F S+ AAD+H Sbjct: 178 PRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTLDMMPENF--SDTRWAADIH 235 Query: 271 LTPDGRFLYATERASNTLSAYRVDPAGGKLTRILNIPTETQP 312 +TPDGR LYA +R ++ ++ + V G L++ PTETQP Sbjct: 236 ITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQP 277 >ybhE [G] COG2706 3-carboxymuconate cyclase Eco Escherichia coli K12 Length = 331 Score = 163 bits (412), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 92/282 (32%), Positives = 153/282 (54%), Gaps = 12/282 (4%) Query: 34 AYVSNADSQDIYVLRLNGDGSVNLIDKVDTGSTVMPLALSPDHKFLYAALRGQPYSVVSY 93 Y+++ +SQ I+V LN +G++ L VD V P+ +SPD ++LY +R + + V++Y Sbjct: 5 VYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVSPDKRYLYVGVRPE-FRVLAY 63 Query: 94 AIDPASGKLKALSKVPLADNMANIATDRSGRYLLAASYSGNRISVNAIGTDGIVQAPPLA 153 I P G L ++ L + +I+TD G+++ SY+ +SV + DG+ P+ Sbjct: 64 RIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYNAGNVSVTRL-EDGL----PVG 118 Query: 154 VIPTGKN---AHSVQVSPDNAFVFASNLGSDVILQYRFDPATGTVTPNSPPSVATKAGAG 210 V+ + HS +SPDN ++ L D I + G + P V T GAG Sbjct: 119 VVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSD-DGHLVAQDPAEVTTVEGAG 177 Query: 211 PRHFVFSPDQRFVYSTNELDATVNTYAYDRQSGTLTLLASDSALPEGFKTSEQLAAADLH 270 PRH VF P++++ Y NEL+++V+ + G + + + +PE F S+ AAD+H Sbjct: 178 PRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTLDMMPENF--SDTRWAADIH 235 Query: 271 LTPDGRFLYATERASNTLSAYRVDPAGGKLTRILNIPTETQP 312 +TPDGR LYA +R ++ ++ + V G L++ PTETQP Sbjct: 236 ITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQP 277 >ECs0795 [G] COG2706 3-carboxymuconate cyclase Ecs Escherichia coli O157:H7 Length = 331 Score = 163 bits (412), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 92/282 (32%), Positives = 153/282 (54%), Gaps = 12/282 (4%) Query: 34 AYVSNADSQDIYVLRLNGDGSVNLIDKVDTGSTVMPLALSPDHKFLYAALRGQPYSVVSY 93 Y+++ +SQ I+V LN +G++ L VD V P+ +SPD ++LY +R + + V++Y Sbjct: 5 VYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVSPDKRYLYVGVRPE-FRVLAY 63 Query: 94 AIDPASGKLKALSKVPLADNMANIATDRSGRYLLAASYSGNRISVNAIGTDGIVQAPPLA 153 I P G L ++ L + +I+TD G+++ SY+ +SV + DG+ P+ Sbjct: 64 RIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYNAGNVSVTRL-EDGL----PVG 118 Query: 154 VIPTGKN---AHSVQVSPDNAFVFASNLGSDVILQYRFDPATGTVTPNSPPSVATKAGAG 210 V+ + HS +SPDN ++ L D I + G + P V T GAG Sbjct: 119 VVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSD-DGHLVAQDPAEVTTVEGAG 177 Query: 211 PRHFVFSPDQRFVYSTNELDATVNTYAYDRQSGTLTLLASDSALPEGFKTSEQLAAADLH 270 PRH VF P++++ Y NEL+++V+ + G + + + +PE F S+ AAD+H Sbjct: 178 PRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTLDMMPENF--SDTRWAADIH 235 Query: 271 LTPDGRFLYATERASNTLSAYRVDPAGGKLTRILNIPTETQP 312 +TPDGR LYA +R ++ ++ + V G L++ PTETQP Sbjct: 236 ITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQP 277 >STM0785 [G] COG2706 3-carboxymuconate cyclase Sty Salmonella typhimurium LT2 Length = 331 Score = 155 bits (393), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 89/279 (31%), Positives = 148/279 (53%), Gaps = 6/279 (2%) Query: 34 AYVSNADSQDIYVLRLNGDGSVNLIDKVDTGSTVMPLALSPDHKFLYAALRGQPYSVVSY 93 Y ++ +SQ I+V LN +G++ L+ VD V P+ +SPD ++LY +R + + V++Y Sbjct: 5 VYTASPESQQIHVWSLNHEGTLTLVQVVDVPGQVQPMVVSPDKRYLYVGVRPE-FRVLAY 63 Query: 94 AIDPASGKLKALSKVPLADNMANIATDRSGRYLLAASYSGNRISVNAIGTDGIVQAPPLA 153 I P G L ++ L + +I+TD GR++ SY+ +SV + DG+ + Sbjct: 64 RIAPDDGALTFAAESALPGSPTHISTDHHGRFVFVGSYNAGNVSVTRL-QDGL-PVELVD 121 Query: 154 VIPTGKNAHSVQVSPDNAFVFASNLGSDVILQYRFDPATGTVTPNSPPSVATKAGAGPRH 213 V+ HS ++PDN ++ L D I + G + P V T GAGPRH Sbjct: 122 VVEGLDGCHSANITPDNRTLWVPALKQDRICLFTLSD-DGHLVAQEPAEVNTVEGAGPRH 180 Query: 214 FVFSPDQRFVYSTNELDATVNTYAYDRQSGTLTLLASDSALPEGFKTSEQLAAADLHLTP 273 VF P++++ Y NEL+++V+ + G + + + +P F S+ AAD+H+TP Sbjct: 181 MVFHPNRQYAYCVNELNSSVDVWQLKNPHGEIECVQTLDMMPADF--SDTRWAADIHITP 238 Query: 274 DGRFLYATERASNTLSAYRVDPAGGKLTRILNIPTETQP 312 DGR LYA +R ++ ++ + V G L+ PTE QP Sbjct: 239 DGRHLYACDRTASLITVFSVSEDGSVLSVEGFQPTEAQP 277