BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0164.p (208 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0418 [E] COG1280 Putative threonine efflux protein Rso Ralsto... 172 6e-43 MA1855 [E] COG1280 Putative threonine efflux protein Mac Methano... 152 6e-37 BH0429 [E] COG1280 Putative threonine efflux protein Bha Bacillu... 150 2e-36 RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralsto... 134 2e-31 BH3495 [E] COG1280 Putative threonine efflux protein Bha Bacillu... 134 2e-31 >RSc0418 [E] COG1280 Putative threonine efflux protein Rso Ralstonia solanacearum Length = 206 Score = 172 bits (435), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 112/208 (53%), Positives = 152/208 (73%), Gaps = 2/208 (0%) Query: 1 MPDLFLFLLAAMTLTLAPGPDNIYVLTRGIAQGRKAGLVAAAGFCSGLIFHTLLAVLGFA 60 MP++ LFLL+++ +T+ PGPDN+ V+ RG +QGRKAGL AAAGF SG +FHT LA LG A Sbjct: 1 MPNVLLFLLSSVAITVMPGPDNLQVIARGASQGRKAGLAAAAGFASGCLFHTTLAALGLA 60 Query: 61 ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASSGIALSADGQGAAAVPLRRIYWQSVLA 120 A++++ P A+ A+++ GAAYL +LGV+ LRS G+ S Q AAA L +++ QSVLA Sbjct: 61 AVLQSAPAAFQAIRWLGAAYLVWLGVQALRS-KGGVGPSVSAQ-AAAPDLWQVFRQSVLA 118 Query: 121 NMLNPKVTLFFIAFLPQFVQREAGYEALQMLILALVFIVQAFVIFSAIALFSGAVGAVFR 180 NMLNPKVTLFF+ FLPQFV +AG+ ALQML+L VF+ Q V+F + A+G R Sbjct: 119 NMLNPKVTLFFVVFLPQFVDAQAGHAALQMLLLGGVFMAQTIVVFGLYGWCAAALGGWMR 178 Query: 181 RRASASVHLNRLAGCAFIGLGIRMALPE 208 R AS+ L+R++GC FIGLG+R+AL + Sbjct: 179 RTPRASLWLDRVSGCIFIGLGLRVALTK 206 >MA1855 [E] COG1280 Putative threonine efflux protein Mac Methanosarcina acetivorans str.C2A Length = 211 Score = 152 bits (383), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 81/211 (38%), Positives = 129/211 (61%), Gaps = 14/211 (6%) Query: 3 DLFLFLLAAMTLTLAPGPDNIYVLTRGIAQGRKAGLVAAAGFCSGLIFHTLLAVLGFAAL 62 L F+ A+ LT PGPD ++VLT+ I+QG+ AG+ A G C+G++ HT A LG +AL Sbjct: 9 QLIYFIAASAALTFLPGPDILFVLTQSISQGKMAGIATATGLCTGILVHTSAAALGISAL 68 Query: 63 IKAYPPAYHALQYAGAAYLAYLGVRTLRSASSGIALSADGQGAAAVPLRR-----IYWQS 117 + A+ ++YAGAAYL YL + L+ + G+ ++ P+R +Y + Sbjct: 69 LYKSALAFEIVKYAGAAYLLYLAWQALKES---------GELVSSAPVRETNALALYRRG 119 Query: 118 VLANMLNPKVTLFFIAFLPQFVQREAGYEALQMLILALVFIVQAFVIFSAIALFSGAVGA 177 + N+LNPKV LFF+AFLPQFV E+G +QM+ L +VF++QA++IFS+I++F+G +G Sbjct: 120 IFMNVLNPKVALFFLAFLPQFVNLESGSVPIQMIFLGIVFLIQAWLIFSSISIFAGIIGD 179 Query: 178 VFRRRASASVHLNRLAGCAFIGLGIRMALPE 208 +R ++N F +G+++AL Sbjct: 180 KIVQRPGIGKYINWGKAGIFTVIGVKLALSH 210 >BH0429 [E] COG1280 Putative threonine efflux protein Bha Bacillus halodurans Length = 207 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 84/208 (40%), Positives = 127/208 (61%), Gaps = 4/208 (1%) Query: 1 MPDLFLFLLAAMTLTLAPGPDNIYVLTRGIAQGRKAGLVAAAGFCSGLIFHTLLAVLGFA 60 + L FL A+ LTL PGPD ++VL + ++Q R+AG+V A G C+GL+ H AV+G + Sbjct: 3 LTSLLSFLGVAVLLTLMPGPDILFVLAQSMSQNRQAGIVTALGLCTGLLVHITAAVVGIS 62 Query: 61 ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASSGIALSADGQGAAAVPLRRIYWQSVLA 120 ALI A+ ++YAGAAYL YL + + G LS D Q A +Y + ++ Sbjct: 63 ALIYQSALAFTVVKYAGAAYLLYLAWKAFQEKGEG--LSIDKQTTLAYG--ALYKKGIIM 118 Query: 121 NMLNPKVTLFFIAFLPQFVQREAGYEALQMLILALVFIVQAFVIFSAIALFSGAVGAVFR 180 N+LNPKV+LFF+A LPQFV AG QML+L +VF++QAF+IFS ++ F+ VG + Sbjct: 119 NVLNPKVSLFFLALLPQFVNSGAGSAPWQMLLLGVVFLIQAFIIFSLVSWFAEKVGQLLM 178 Query: 181 RRASASVHLNRLAGCAFIGLGIRMALPE 208 R + ++R+ G +G+++A + Sbjct: 179 RSSFIKNQMHRIKGGLLALIGLQVAFSK 206 >RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralstonia solanacearum Length = 215 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 82/204 (40%), Positives = 116/204 (56%), Gaps = 1/204 (0%) Query: 3 DLFLFLLAAMTLTLAPGPDNIYVLTRGIAQGRKAGLVAAAGFCSGLIFHTLLAVLGFAAL 62 +L +F+LA + L + PGPD Y++ R ++QGR AGL+++ G +G H L G AL Sbjct: 8 NLPVFMLAVLLLNVTPGPDTAYIVGRSVSQGRAAGLMSSLGVSAGCCVHVLAVAFGLTAL 67 Query: 63 IKAYPPAYHALQYAGAAYLAYLGVRTLRS-ASSGIALSADGQGAAAVPLRRIYWQSVLAN 121 + A A+ ++ GAAYL YLG R L + A S A A A PLR ++ Q L N Sbjct: 68 LAASAVAFTVIKAIGAAYLIYLGGRMLLAPAGSDDAPVAQAVATARRPLRALFMQGFLTN 127 Query: 122 MLNPKVTLFFIAFLPQFVQREAGYEALQMLILALVFIVQAFVIFSAIALFSGAVGAVFRR 181 +LNPKV LFF++F PQFV A ++A L+L VFIV + S +A + +V Sbjct: 128 VLNPKVVLFFLSFFPQFVDPGASHKAAAFLVLGAVFIVMSTAWNSLVAWVAASVTRRVAG 187 Query: 182 RASASVHLNRLAGCAFIGLGIRMA 205 + L+R+ G AFIGLG R+A Sbjct: 188 KPGIKRWLDRVVGTAFIGLGARLA 211 >BH3495 [E] COG1280 Putative threonine efflux protein Bha Bacillus halodurans Length = 208 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 70/203 (34%), Positives = 118/203 (58%), Gaps = 4/203 (1%) Query: 6 LFLLAAMTLTLAPGPDNIYVLTRGIAQGRKAGLVAAAGFCSGLIFHTLLAVLGFAALIKA 65 LF+ +A+ L + PGPD ++ +T+G+ GRKAG++ A G G I HTL AVLG + +I+ Sbjct: 9 LFMGSAIMLIIIPGPDLVFTITQGMTNGRKAGVITAMGLSLGNIVHTLAAVLGLSLIIQT 68 Query: 66 YPPAYHALQYAGAAYLAYLGVRTLRSASSGIALSADGQGAAAVPLRRIYWQSVLANMLNP 125 + + AGA YL YL + L+ I ++++ L+ ++ + ++ N+LNP Sbjct: 69 SVVVFTIFKLAGAMYLFYLAYKALKHRKEEIRVNSEKTN----DLKGLFLRGLIMNVLNP 124 Query: 126 KVTLFFIAFLPQFVQREAGYEALQMLILALVFIVQAFVIFSAIALFSGAVGAVFRRRASA 185 KV +F++ FLPQFV ++ +LQ+ IL L+FI+ +IFS FSG + + Sbjct: 125 KVAIFYLTFLPQFVNYQSANISLQLCILGLIFIIMTAIIFSIFGYFSGTFRDRLLKNSRF 184 Query: 186 SVHLNRLAGCAFIGLGIRMALPE 208 + ++N A FIGLG+++ + Sbjct: 185 NEYMNIAATIIFIGLGLKLMTTQ 207