BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0040.p (528 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1363 [C] COG2225 Malate synthase Rso Ralstonia solanacearum 920 0.0 DRA0277 [C] COG2225 Malate synthase Dra Deinococcus radiodurans 651 0.0 CC1765 Non COG 532 e-151 YPO3726 [C] COG2225 Malate synthase Ype Yersinia pestis 525 e-148 YNL117w [C] COG2225 Malate synthase Sce Saccharomyces cerevisiae 518 e-146 >RSc1363 [C] COG2225 Malate synthase Rso Ralstonia solanacearum Length = 529 Score = 920 bits (2377), Expect = 0.0, Method: Compositional matrix adjust. Identities = 438/526 (83%), Positives = 477/526 (90%), Gaps = 1/526 (0%) Query: 3 QLKLPTGMEISGEIKPGYENVLTFEALSLVAKLSRAFEGRRQELLAARAERVKRLDAGER 62 +L LP GMEI EI P YE++LT EAL+LVA+L RAFE RRQ+ LA RAERVKRLDAGER Sbjct: 5 ELTLPQGMEIKAEILPAYEDILTPEALALVARLHRAFEPRRQQRLAERAERVKRLDAGER 64 Query: 63 PDFLKETESIRSGDWKIAPIPEALKCRRVEITGPVERKMVINAFNSGADSYMTDFEDSNS 122 PDFL ET IR GDW IAPIP AL+CRRVEITGPVE KMVINAFNSGADSYMTDFEDSN+ Sbjct: 65 PDFLPETRHIREGDWTIAPIPPALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSNA 124 Query: 123 PVWDNQISGQINLYDAIRRTISLESNGKSYKLNDKIATLVVRPRGWHLDEKHVTLDGKRI 182 P W NQI GQ+NL AIRRT++LES GKSYKLNDKIATL VRPRGWHLDEKHVT+DG+R+ Sbjct: 125 PSWHNQIQGQVNLKAAIRRTLTLESGGKSYKLNDKIATLQVRPRGWHLDEKHVTVDGQRV 184 Query: 183 SGGIFDFALFLFHNAKEQLARGAGPYFYLPKMESHLEARLWNDIFVMAQNEVGLPQGTIK 242 SGGIFDFALFLFHNA+EQLARGAGP+FYLPKMESHLEARLWND+F+ AQ+ +GLP+GTIK Sbjct: 185 SGGIFDFALFLFHNAREQLARGAGPFFYLPKMESHLEARLWNDVFIAAQDALGLPRGTIK 244 Query: 243 ATVLIETITAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKNDKDFCLADRAKVTMT 302 ATVLIETI AAFEM+EILYELREHSAGLNAGRWDYIFSCIKKFK D++FCLADRA+VTMT Sbjct: 245 ATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKVDQNFCLADRARVTMT 304 Query: 303 SPFMRAYALLLLKTCHKRGAPAIGGMSALIPIKNDPEKNAIAMQGIINDKRRDATDGYDG 362 SPFMRAYALLLLKTCHKR APA+GGMSALIPIKNDPEKNA+AMQGIINDK+RDATDGYDG Sbjct: 305 SPFMRAYALLLLKTCHKRNAPAMGGMSALIPIKNDPEKNAVAMQGIINDKKRDATDGYDG 364 Query: 363 GWVAHPGLVEASMKEFVAVLGDKPNQFEKQRPDVEVKAADLLNFQPETPITEAGLRYNIN 422 GWVAHPGLVEA+MKEFV VLGD PNQ KQRPDV V AADLLNFQPE PITEAGLR NIN Sbjct: 365 GWVAHPGLVEAAMKEFVDVLGDAPNQIGKQRPDVNVAAADLLNFQPEQPITEAGLRMNIN 424 Query: 423 VGIHYLGAWLAGNGCVPIHNLMEDAATAEISRAQVWQWIRSAKGNLEDGRKVTADMVRAM 482 VGIHYLGAWLAGNGCVPIHNLMEDAATAEISR+QVWQWIRS KG LEDG KVTA++VR + Sbjct: 425 VGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGTLEDGTKVTAELVRKL 484 Query: 483 IPEELAKVKQVAGDGPTYDRAAKIFEEMSTSETFAEFLTLPLYEEI 528 IPEELAKVK G +DRAA+IFE+MSTSE+F+EFLTLPLYEE+ Sbjct: 485 IPEELAKVKADGAVG-HFDRAAQIFEQMSTSESFSEFLTLPLYEEL 529 >DRA0277 [C] COG2225 Malate synthase Dra Deinococcus radiodurans Length = 521 Score = 651 bits (1679), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/530 (61%), Positives = 400/530 (75%), Gaps = 12/530 (2%) Query: 1 MTQLKLPTGMEISGEIKPGYENVLTFEALSLVAKLSRAFEGRRQELLAARAERVKRLDAG 60 MT L LP GM I+ IKP Y +LT EAL+ VA+L R FE RR+EL+ R ER +RLDAG Sbjct: 1 MTDLNLPAGMTINAPIKPEYAEILTPEALAFVAELHRRFEPRRRELMQKREERQQRLDAG 60 Query: 61 ERPDFLKETESIRSGDWKIAPIPEALKCRRVEITGPVERKMVINAFNSGADSYMTDFEDS 120 E PDFL ET IR+GDWKI+P+P+ALK RRVEITGPV+RKM+INA NSGA +M DFED+ Sbjct: 61 ELPDFLPETAEIRAGDWKISPLPDALKDRRVEITGPVDRKMIINALNSGAKMFMADFEDA 120 Query: 121 NSPVWDNQISGQINLYDAIRRTISLESNGKSYKLNDKIATLVVRPRGWHLDEKHVTLDGK 180 +SP WDN + GQINL DA+R TISLE NGKSYKLND ATL+VRPRGWHL EKHV +DG+ Sbjct: 121 SSPTWDNCVQGQINLRDAVRGTISLEQNGKSYKLNDTTATLLVRPRGWHLPEKHVQVDGE 180 Query: 181 RISGGIFDFALFLFHNAKEQLARGAGPYFYLPKMESHLEARLWNDIFVMAQNEVGLPQGT 240 + G FDF LF FHNAKEQLARG GPYFYLPKMESHLEARLWND+F A++ + +P+G+ Sbjct: 181 TLYGAFFDFGLFFFHNAKEQLARGKGPYFYLPKMESHLEARLWNDVFNYAEDTIQIPRGS 240 Query: 241 IKATVLIETITAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKNDKDFCLADRAKVT 300 I+ATVLIETI AAFEM+EILYELREHSAGLN GRWDYIFS IKK +N D L DRAKVT Sbjct: 241 IRATVLIETIVAAFEMDEILYELREHSAGLNCGRWDYIFSYIKKLRNHSDRVLPDRAKVT 300 Query: 301 MTSPFMRAYALLLLKTCHKRGAPAIGGMSALIPIKNDPEKNAIAMQGIINDKRRDATDGY 360 M P M+ Y+ L ++TCHKRGAPAIGGMSA IP+K D EKNA A + + DK R+AT+G+ Sbjct: 301 MAVPMMQNYSKLAIQTCHKRGAPAIGGMSAFIPVKGDEEKNAAAFEQVRVDKEREATNGH 360 Query: 361 DGGWVAHPGLVEASMKEFVAVLGDKPNQF--EKQRPDVEVKAADLLNFQPETPITEAGLR 418 DG WVAHPG+V+ + + F ++ +PNQ +KQ+ D + A DLL PE +TEAG+R Sbjct: 361 DGTWVAHPGMVQLATEVFDRIM-PQPNQIGSDKQK-DFSLTATDLLQ-APEGSVTEAGVR 417 Query: 419 YNINVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRAQVWQWIRSAKGNLEDGRKVTADM 478 NINVGI YL AWL G+G VPIHNLMEDAATAEISRAQ+WQW++ LEDGR + + Sbjct: 418 TNINVGIQYLAAWLRGSGAVPIHNLMEDAATAEISRAQLWQWLKQGV-TLEDGRTLDQGL 476 Query: 479 VRAMIPEELAKVKQVAGDGPTYDRAAKIFEEMSTSETFAEFLTLPLYEEI 528 + EE K+ G + A+ +F++ +T +T +FLTLP Y+++ Sbjct: 477 FDQLYTEETDKL------GAEFADASALFKQTATGDTLVDFLTLPGYQQL 520 >CC1765 Non COG Length = 525 Score = 532 bits (1370), Expect = e-151, Method: Compositional matrix adjust. Identities = 262/525 (49%), Positives = 353/525 (67%), Gaps = 6/525 (1%) Query: 4 LKLPTGMEISGEIKPGYENVLTFEALSLVAKLSRAFEGRRQELLAARAERVKRLDAGERP 63 L + ++++ ++ VLT EAL+ VA L R F+ RR++LL AR R R DAGE P Sbjct: 3 LDIAANIQVTAPVEGRAAEVLTPEALAFVADLHRRFDARRRQLLEARQARQARFDAGELP 62 Query: 64 DFLKETESIRSGDWKIAPIPEALKCRRVEITGPVERKMVINAFNSGADSYMTDFEDSNSP 123 DFL ET SIRS DW IA IP L RRVEITGPV RKM+INA N GA +M DFED+ +P Sbjct: 63 DFLDETASIRSADWTIAAIPSDLLDRRVEITGPVNRKMIINALNCGAKVFMADFEDATAP 122 Query: 124 VWDNQISGQINLYDAIRRTISL--ESNGKSYKLNDKIATLVVRPRGWHLDEKHVTLDGKR 181 W N + GQINL D + ++ +GK+Y + A L +RPRGWHL E+H+ +DG+ Sbjct: 123 TWANVVEGQINLKDRWQGELTHVDAKSGKAYAMGPTSAVLKIRPRGWHLMERHLEVDGQA 182 Query: 182 ISGGIFDFALFLFHNAKEQLARGAGPYFYLPKMESHLEARLWNDIFVMAQNEVGLPQGTI 241 ++G +FDF L+ FHNAK +A+G+GPYFYLPK+ESHLEARLWND+F+ AQ + LP GTI Sbjct: 183 VAGALFDFGLYAFHNAKAAIAKGSGPYFYLPKLESHLEARLWNDVFIRAQEVLDLPVGTI 242 Query: 242 KATVLIETITAAFEMEEILYELREHSAGLNAGRWDYIFSCIKKFKNDKDFCLADRAKVTM 301 KATVLIETI AAFEM+EIL+EL++H GLN GRWDYIFS IK+ + F DR+ + M Sbjct: 243 KATVLIETIPAAFEMDEILFELKDHIVGLNCGRWDYIFSFIKRLGRREAFLTPDRSAMVM 302 Query: 302 TSPFMRAYALLLLKTCHKRGAPAIGGMSALIPIKNDPEKNAIAMQGIINDKRRDATDGYD 361 F+ AY+L L++TCH+RGA A+GGM+A IP+K D N A + DK R+A G+D Sbjct: 303 GKAFLGAYSLKLIQTCHRRGAFAMGGMAAQIPVKGDEAANNAAFAKVRADKEREAGAGHD 362 Query: 362 GGWVAHPGLVEASMKEFVAVLGDKPNQFEKQRPDVEVKAADLLNFQPETPITEAGLRYNI 421 G WVAHP LV +M+ F ++ PNQ +++ D+ + A +L E TEAG+R NI Sbjct: 363 GTWVAHPDLVPVAMEVFNRLM-PTPNQLDRKLDDLSITQAQMLELH-EGSRTEAGVRENI 420 Query: 422 NVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRAQVWQWIRSAKGNLEDGRKVTADMVRA 481 VG+ Y AW+ G G VP++NLMEDAATAEI R Q+WQW+R L+DGR +++++ + Sbjct: 421 RVGVRYTQAWIEGRGAVPLYNLMEDAATAEICRTQLWQWVRLGAA-LDDGRVLSSELFVS 479 Query: 482 MIPEELAKVKQVAGDGPTYDRAAKIFEEMSTSETFAEFLTLPLYE 526 + +E++ +++ P + AA IF M S+ EFLT+P YE Sbjct: 480 LFSQEMSDLRREFA-SPRLEEAAHIFTRMVLSDDLEEFLTIPAYE 523 >YPO3726 [C] COG2225 Malate synthase Ype Yersinia pestis Length = 532 Score = 525 bits (1351), Expect = e-148, Method: Compositional matrix adjust. Identities = 268/511 (52%), Positives = 344/511 (67%), Gaps = 11/511 (2%) Query: 22 NVLTFEALSLVAKLSRAFEGRRQELLAARAERVKRLDAGERPDFLKETESIRSGDWKIAP 81 VL +A+ + +L F R +LLA RA + +D G PDF+ ET SIR GDWKI Sbjct: 22 QVLPDKAIEFLTELVVKFSESRNQLLATRASWQQTIDQGALPDFILETNSIRDGDWKIQG 81 Query: 82 IPEALKCRRVEITGPVERKMVINAFNSGADSYMTDFEDSNSPVWDNQISGQINLYDAIRR 141 IP L+ RRVEITGPVERKMVINA N+ +M DFEDS +P WD I GQINL+DA++ Sbjct: 82 IPADLRDRRVEITGPVERKMVINALNANVKVFMADFEDSLAPSWDKVIDGQINLHDAVKG 141 Query: 142 TISLES-NGKSYKLNDKIATLVVRPRGWHLDEKHVTLDGKRISGGIFDFALFLFHNAKEQ 200 TIS + +GK Y+L A L+ R RG HL EKHV + I GG+FDFAL+ +HN ++ Sbjct: 142 TISYTNESGKVYQLKPNPAVLIARVRGLHLPEKHVKWQDEAIPGGLFDFALYFYHNYQQL 201 Query: 201 LARGAGPYFYLPKMESHLEARLWNDIFVMAQNEVGLPQGTIKATVLIETITAAFEMEEIL 260 LA+G+GPYFYLPKM+S+ EA W+D+F + GL QGTIKATVLIET+ A F+M+EIL Sbjct: 202 LAKGSGPYFYLPKMQSYQEAAWWSDVFNFTEQRFGLSQGTIKATVLIETLPAVFQMDEIL 261 Query: 261 YELREHSAGLNAGRWDYIFSCIKKFKNDKDFCLADRAKVTMTSPFMRAYALLLLKTCHKR 320 Y LR H GLN GRWDYIFS IK KN D L DR VTM PF+ AY+ LL+KTCHKR Sbjct: 262 YHLRHHIVGLNCGRWDYIFSYIKTLKNHPDRVLPDRQSVTMNKPFLSAYSRLLIKTCHKR 321 Query: 321 GAPAIGGMSALIPIKNDPEKNAIAMQGIINDKRRDATDGYDGGWVAHPGLVEASMKEFVA 380 GA A+GGM+A IP K D EKN + + + DK +A++G+DG WVAHPGL + M F Sbjct: 322 GALAMGGMAAFIPDK-DAEKNKLVLDKVRADKELEASNGHDGTWVAHPGLADTVMDVFNK 380 Query: 381 VLGDKPNQFEKQR-PDVEVKAADLLNFQPET-PITEAGLRYNINVGIHYLGAWLAGNGCV 438 VLG +PNQ E R D + AA+LL +P + TEAG+R NI V + Y+ AW++GNGCV Sbjct: 381 VLGSRPNQLEVSREQDKPITAAELL--EPCSGERTEAGMRANIRVAVQYIEAWISGNGCV 438 Query: 439 PIHNLMEDAATAEISRAQVWQWIRSAKGNLEDGRKVTADMVRAMIPEELAKVKQVAG--- 495 PI+ LMEDAATAEISR +WQWI K +L +G+ VT ++ R+M+ EE+ VK G Sbjct: 439 PIYGLMEDAATAEISRTSIWQWIHHQK-SLSNGQTVTKELFRSMLSEEIQVVKLELGAER 497 Query: 496 -DGPTYDRAAKIFEEMSTSETFAEFLTLPLY 525 D ++ AA++ E ++T + +FLTLP Y Sbjct: 498 FDRGRFEEAARLMERITTQDELIDFLTLPGY 528 >YNL117w [C] COG2225 Malate synthase Sce Saccharomyces cerevisiae Length = 554 Score = 518 bits (1334), Expect = e-146, Method: Compositional matrix adjust. Identities = 258/515 (50%), Positives = 351/515 (68%), Gaps = 11/515 (2%) Query: 22 NVLTFEALSLVAKLSRAFEGRRQELLAARAERVKRLDAGE-RPDFLKETESIRSG-DWKI 79 ++LT +AL + L R F +R++LL R K+LD+G DFL ET +IR+ W+ Sbjct: 30 DILTKDALEFIVLLHRTFNNKRKQLLENRQVVQKKLDSGSYHLDFLPETANIRNDPTWQG 89 Query: 80 APIPEALKCRRVEITGPVERKMVINAFNSGADSYMTDFEDSNSPVWDNQISGQINLYDAI 139 + L R EITGP R M+INA N+ ++YMTDFEDS SP W+N + GQ+NLYDAI Sbjct: 90 PILAPGLINRSTEITGPPLRNMLINALNAPVNTYMTDFEDSASPTWNNMVYGQVNLYDAI 149 Query: 140 RRTISLESNGKSYKLNDKIA---TLVVRPRGWHLDEKHVTLDGKRISGGIFDFALFLFHN 196 R I ++ KSYKLN +A T++VRPRGWH+ EKH+ +D + IS IFDF L+ +HN Sbjct: 150 RNQIDFDTPRKSYKLNGNVANLPTIIVRPRGWHMVEKHLYVDDEPISASIFDFGLYFYHN 209 Query: 197 AKEQLARGAGPYFYLPKMESHLEARLWNDIFVMAQNEVGLPQGTIKATVLIETITAAFEM 256 AKE + G GPYFYLPKME HLEA+LWND+F +AQ+ +G+P+GTI+ATVLIET+ AAF+M Sbjct: 210 AKELIKLGKGPYFYLPKMEHHLEAKLWNDVFCVAQDYIGIPRGTIRATVLIETLPAAFQM 269 Query: 257 EEILYELREHSAGLNAGRWDYIFSCIKKFKNDKDFCLADRAKVTMTSPFMRAYALLLLKT 316 EEI+Y+LR+HS+GLN GRWDYIFS IK+ +ND + L +R +VTMTSPFM AY L+ T Sbjct: 270 EEIIYQLRQHSSGLNCGRWDYIFSTIKRLRNDPNHILPNRNQVTMTSPFMDAYVKRLINT 329 Query: 317 CHKRGAPAIGGMSALIPIKNDPEKNAIAMQGIINDKRRDATDGYDGGWVAHPGLVEASMK 376 CH+RG A+GGM+A IPIK+DP N AM + NDK R+ T+G+DG WVAHP L + Sbjct: 330 CHRRGVHAMGGMAAQIPIKDDPAANEKAMTKVRNDKIRELTNGHDGSWVAHPALAPICNE 389 Query: 377 EFVAVLGDKPNQFEKQRPDVEVKAADLLNFQ-PETPITEAGLRYNINVGIHYLGAWLAGN 435 F+ + PNQ P+ V AA+LL + P IT G+ N+++G+ Y+ AWL G+ Sbjct: 390 VFINM--GTPNQI-YFIPENVVTAANLLETKIPNGEITTEGIVQNLDIGLQYMEAWLRGS 446 Query: 436 GCVPIHNLMEDAATAEISRAQVWQWIRSAKGNLEDGRKVTADMVRAMIPEELAKVKQVA- 494 GCVPI+NLMEDAATAE+SR Q++QW++ + G KVT ++ ++ E++ ++ + + Sbjct: 447 GCVPINNLMEDAATAEVSRCQLYQWVKHGVTLKDTGEKVTPELTEKILKEQVERLSKASP 506 Query: 495 -GDGPTYDRAAKIFEEMSTSETFAEFLTLPLYEEI 528 GD + AAK F E F+EFLT LY+EI Sbjct: 507 LGDKNKFALAAKYFLPEIRGEKFSEFLTTLLYDEI 541