BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0001.p (452 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2451 [M] COG3264 Small-conductance mechanosensitive channel R... 263 5e-70 SMa1582 [M] COG3264 Small-conductance mechanosensitive channel S... 162 1e-39 AGc3014 [M] COG3264 Small-conductance mechanosensitive channel A... 129 2e-29 YPO3129 [M] COG3264 Small-conductance mechanosensitive channel Y... 114 3e-25 mll1272 [M] COG3264 Small-conductance mechanosensitive channel M... 113 8e-25 >RSc2451 [M] COG3264 Small-conductance mechanosensitive channel Rso Ralstonia solanacearum Length = 447 Score = 263 bits (673), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 163/401 (40%), Positives = 250/401 (62%), Gaps = 7/401 (1%) Query: 37 WLRARFV---LPGDGEAQSLVMQIGVKSFARVLSPLLALGLLTGAYYILKRGHHAVTLWE 93 WL AR + L S ++ S R + PLL L+ A Y L G ++++ Sbjct: 42 WLAARPIARRLHARHAGDSFALRFAWASLERAVFPLLGWLLVLAARYALT-GVMPISVFR 100 Query: 94 IMFPLTIAQVAMRFVFYVLRGIFVKDATVGALLHLSEKVIALLVWLCVVLWITGLWPDFV 153 + + FVFYVLR + + + +L L E+V+ L+W+ +VL++ G+ D V Sbjct: 101 LAVVPLFGLAMLYFVFYVLRRVLSANGDLHGMLVLVERVLTTLMWIGMVLYVVGVLSDVV 160 Query: 154 DYLDTTTFPLA-RHKVSLLTMLQGAASVLVTLIIALWIGTVVENRLMKFSGMHSSLRTVV 212 YL+ F + + KV+L ML +L+T+++A+W G+ +++R+ + + + ++L+ V+ Sbjct: 161 SYLEGIQFSVGGKQKVNLAAMLMAVVWILLTVLVAMWFGSWLDSRITRAAAIDANLKVVL 220 Query: 213 ARMMRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERS 272 +R+ +A L+L++ L+SLSLVGIDLTVLSVFGGALGVG+G GLQKIAS+YVSGF+ILLERS Sbjct: 221 SRVAKAALLLVSLLLSLSLVGIDLTVLSVFGGALGVGLGFGLQKIASNYVSGFIILLERS 280 Query: 273 MVIGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRLAT 332 + +GD + V Y G VTQI TRYT++ DG ++ +PNEL V+ VQN++ VR+A Sbjct: 281 LKLGDQITVSTYTGIVTQIRTRYTVVRNGDG-DTFVPNELMVAQAVQNHN-ERGTVRVAV 338 Query: 333 QLTILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGLELELGFWITDPENGR 392 ++ Y D E VL ++ A GV RV AP A L G+E ELG WI DP NG+ Sbjct: 339 RVQAAYPADPEVVLGLLVACAEGVPRVLADPAPAAFLAAFADSGIEYELGVWIADPHNGK 398 Query: 393 LNVLSDVNRAIWAAFKQHGIQVAHPKRDIRIMDERSFRQST 433 L V SD+NRAI+ FK G+++ +P+R++R++ + +QS+ Sbjct: 399 LGVQSDLNRAIYKRFKAAGVEIPYPQREVRLLGPLAVQQSS 439 >SMa1582 [M] COG3264 Small-conductance mechanosensitive channel Sme Sinorhizobium meliloti Length = 432 Score = 162 bits (411), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 112/345 (32%), Positives = 191/345 (55%), Gaps = 11/345 (3%) Query: 78 AYYILKRGHHAVTLWEIMFPLTIAQVAMRFVFYVLRGIFVKDATVGALLHLSEKVIALLV 137 A ILK+G W I L++A A F+ VL I +++ T+ +++A++ Sbjct: 84 ASVILKQGTWPSQRWLIATALSLA--AAWFIISVLTKI-IRNPTL-------SRLVAMVS 133 Query: 138 WLCVVLWITGLWPDFVDYLDTTTFPLARHKVSLLTMLQGAASVLVTLIIALWIGTVVENR 197 W + ++ TGL + LD L ++SLL +L+ + + +A+++G V+ + Sbjct: 134 WGYLAVYATGLDGPILSALDAAAVNLGVMRLSLLIVLKAVVLTVALIWVAVFVGNVLAHW 193 Query: 198 LMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKI 257 + + + S + +++++++ LI+IA ++LS GIDLT L+VF GA+GVG+G GLQK+ Sbjct: 194 VQRSGDLSPSFKVLISKVIKIALIMIAGAIALSATGIDLTALTVFSGAVGVGVGFGLQKV 253 Query: 258 ASSYVSGFVILLERSMVIGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNP 317 S+++SG +ILL++S+ GD + + + FG + + R+ + DG E ++PNE F+S Sbjct: 254 VSNFISGIIILLDKSIKPGDTITLGETFGSIRDLRARFVSVITRDGKEYLIPNEDFISQQ 313 Query: 318 VQNYSLTHRIVRLATQLTILYQDDVETVLSIMEQAALGVQR-VSQQTAPQALLIKIGADG 376 V N+S + VR+ Y D V+ I + A V R V+ AP + GA Sbjct: 314 VVNWSFSSDYVRIDVDFGTSYDSDPHEVVRIAVETASAVPRVVNDYNAPVCWMTAFGASS 373 Query: 377 LELELGFWITDPENGRLNVLSDVNRAIWAAFKQHGIQVAHPKRDI 421 L+ L FWI+DP NG NV V A+W +FK+ G+ + P R+I Sbjct: 374 LDFRLRFWISDPANGLTNVRGQVLMALWDSFKEAGVSIPFPHREI 418 >AGc3014 [M] COG3264 Small-conductance mechanosensitive channel Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 881 Score = 129 bits (324), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 108/409 (26%), Positives = 188/409 (45%), Gaps = 35/409 (8%) Query: 21 LVVIALSLVGGIGLSRWLRARFVLPGDGEAQSLVMQIGVKSFARVLSPLLALGLLTGAYY 80 L++I L G +GL+R+L + V G A M IG+ S + +S A G Y Sbjct: 443 LILIGACLTGYVGLARFLATQIVATG---AVLATMYIGILS-GKAISRQGAFGESLAGRY 498 Query: 81 ILKRGHHAVTLWEIMFPLTIAQVAMRFVFYVLRGIFVKDATVGALLHLSEKVIALLVWLC 140 + +R ++ VA+ G L V+AL + Sbjct: 499 LARR-------------FSLGPVALD--------------QAGLAAGLGIYVVALAFGVP 531 Query: 141 VVLWITGLWPDFVD---YLDTTTFPLARHKVSLLTMLQGAASVLVTLIIALWIGTVVENR 197 ++L+ G P ++ Y T + +SL+ + G + II W ++N Sbjct: 532 LILFSWGFQPGDIESWAYRLLTGITVGNASISLIGLFGGVLVFAIGYIITRWFQKWLDNN 591 Query: 198 LMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKI 257 +M + + +R V + L I +A + +S G++L+ L++ AL VGIG GLQ I Sbjct: 592 VMARGQVDAGVRNSVKTGIGYLGIAVAAIFGVSSAGLNLSSLALVASALSVGIGFGLQNI 651 Query: 258 ASSYVSGFVILLERSMVIGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNP 317 S++VSG ++L+ER +GD V G V +++ R T +E G ++PN F+++ Sbjct: 652 VSNFVSGLILLVERPFKVGDWVVTGTTEGTVKRLSVRATEIETFRGQSIIVPNSEFINSS 711 Query: 318 VQNYSLTHRIVRLATQLTILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGL 377 V N++ +RI+R +++ Y D + V+ I+ + V + P ++ G L Sbjct: 712 VGNWTHRNRIMRAEIPVSVAYDSDPQQVMDILLELVRAQPPVLRNPEPHVEFLRFGDFSL 771 Query: 378 ELELGFWITDPENGRLNVLSDVNRAIWAAFKQHGIQVAHPKRDIRIMDE 426 + EL F + D NG L V + + AI F++ GI + P+R++ I E Sbjct: 772 DFELRFHLADLSNG-LAVKNALRIAILHRFREEGIAIPFPQRNLNIHVE 819 >YPO3129 [M] COG3264 Small-conductance mechanosensitive channel Ype Yersinia pestis Length = 1139 Score = 114 bits (286), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 85/303 (28%), Positives = 149/303 (49%), Gaps = 24/303 (7%) Query: 135 LLVWLCVVLWITGLWPDFV---DYLDTT-----TFPLARHKVSLLTMLQGAASVLVTLII 186 L++ WI W D + YLD+ T +A V+ L L+ L++ Sbjct: 808 FLIFATCFYWI---WADLITVISYLDSISLWHYTATVAGASVTQAVTLGNMLVALMALVV 864 Query: 187 AL--------WIGTVVENRLMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTV 238 A + VV +RL G ++ T++ ++ A + + +LS +G+ Sbjct: 865 AYVLTRNLPGLLEVVVLSRLQLRQGTSYAITTILTYLITA----VGGITALSSLGVSWDK 920 Query: 239 LSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKYFGKVTQINTRYTIL 298 L AL VG+G GLQ+I +++VSG +IL ER + IGD + + Y G V++I R T + Sbjct: 921 LQWLVAALSVGLGFGLQEIFANFVSGLIILFERPVRIGDTITIGTYSGTVSRIRIRATTI 980 Query: 299 EGLDGIESVLPNELFVSNPVQNYSLTHRIVRLATQLTILYQDDVETVLSIMEQAALGVQR 358 D E ++PN+ FV+ + N+SL+ I R+ ++ + Y D+E V ++ QAA R Sbjct: 981 TDFDRKEVIVPNKAFVTERLINWSLSDTITRIIIKVGVAYGSDLEKVKEVLLQAAHANSR 1040 Query: 359 VSQQTAPQALLIKIGADGLELELGFWITDPENGRLNVLSDVNRAIWAAFKQHGIQVAHPK 418 V PQ + G+ L+ EL ++ + + R + ++NRAI +++ I +A + Sbjct: 1041 VMTDPEPQVFFLNFGSSTLDHELRLYVRELRD-RSYTVDELNRAIDKLCRENDINIAFNQ 1099 Query: 419 RDI 421 D+ Sbjct: 1100 LDV 1102 >mll1272 [M] COG3264 Small-conductance mechanosensitive channel Mlo Mesorhizobium loti Length = 850 Score = 113 bits (283), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 78/303 (25%), Positives = 149/303 (49%), Gaps = 4/303 (1%) Query: 122 VGALLHLSEKVIALLVWLCVVLWITGLWPDFVD---YLDTTTFPLARHKVSLLTMLQGAA 178 +G ++ ++ ++ +LV+L ++L + G P + Y T + +S+ +L G Sbjct: 499 LGLVVSVAINLMIVLVFLPLILLMWGFQPGDIQAWAYKLATGITIGSVTISVTGILSGII 558 Query: 179 SVLVTLIIALWIGTVVENRLMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTV 238 ++ + W ++ +M + + +R + + + +A LV +S GIDL+ Sbjct: 559 VFIIGYFLTRWFQGWLDGSVMARGKVDTGVRNSIRLAVGYAGVAVAGLVGISAAGIDLSS 618 Query: 239 LSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKYFGKVTQINTRYTIL 298 L++ GAL +GIG GLQ + S++VSG ++L ER +GD + G V +I+ R T + Sbjct: 619 LALVAGALSLGIGFGLQNVVSNFVSGLILLAERPFKVGDWIVAGDVSGTVKKISVRATEI 678 Query: 299 EGLDGIESVLPNELFVSNPVQNYSLTHRIVRLATQLTILYQDDVETVLSIMEQAALGVQR 358 E +LPN ++N V N++ +++ R+ ++ + Y DV+ V +I+ + A Sbjct: 679 ETFQRQSVILPNSNLINNAVGNWTHRNKLGRIDIKVGVAYGSDVKQVHAILLEIARSHPM 738 Query: 359 VSQQTAPQALLIKIGADGLELELGFWITDPENGRLNVLSDVNRAIWAAFKQHGIQVAHPK 418 V + P L G LE E+ ++ D NG + V +DV A+ F I++ Sbjct: 739 VLKNPEPFVLFSNFGPAALEFEIRVFLADVTNGNI-VQNDVRFAVLEKFSDQHIEIPSTP 797 Query: 419 RDI 421 R + Sbjct: 798 RAV 800