BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0109.p (244 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2227 [P] COG0428 Predicted divalent heavy-metal cations trans... 270 3e-72 YIL023c [P] COG0428 Predicted divalent heavy-metal cations trans... 87 3e-17 SPAP8A3.03 [P] COG0428 Predicted divalent heavy-metal cations tr... 75 1e-13 TM1738 [P] COG0428 Predicted divalent heavy-metal cations transp... 64 3e-10 BB0219 Non COG 64 4e-10 >RSc2227 [P] COG0428 Predicted divalent heavy-metal cations transporter Rso Ralstonia solanacearum Length = 268 Score = 270 bits (689), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 161/246 (65%), Positives = 199/246 (80%), Gaps = 4/246 (1%) Query: 3 SITAAAVFSFALLSKVVERMVSLSVGIMLSTSLLHALPEAFES----QAEPHTLFAVLLG 58 S+ AA S +L++VV+RMVS SVG++L+TSLLH+LPEAFES + H LFA LL Sbjct: 21 SMAGAAALSLTVLARVVDRMVSFSVGVLLATSLLHSLPEAFESHHGIEPNTHALFATLLA 80 Query: 59 GLLVFFLLEKTAILRHSHHHEDDGHGHHHGHDKKEAGKAGWMILVGDGLHNFTDGILIAA 118 GLL FFLLEK A++RHSHHHE DGHGHHHGHD++EAG++G MILVGDGLHNF+DGI+IAA Sbjct: 81 GLLGFFLLEKIALIRHSHHHEGDGHGHHHGHDRQEAGRSGLMILVGDGLHNFSDGIVIAA 140 Query: 119 AFLADPHLGLVTGLAIIAHEIPQEIGDFIVLLNAGFSRTRAYVYNLICSLMAVAGGVLGY 178 AFLAD +G+VT LAI AHEIPQEIGDF+VLLNAGFS+TRA+VYNLICS+ A+ G VLGY Sbjct: 141 AFLADTRVGIVTALAIAAHEIPQEIGDFMVLLNAGFSKTRAFVYNLICSVCALVGAVLGY 200 Query: 179 FMLERGMEWIPFVLVFASSGFIYIAVSDLMPQMQRRATLRETIPQVILIAAGVLIVLALT 238 ++L+R WIP+VLV ASS FIYIAV DLMPQM+RR +E+ Q+ L+ AG++++ LT Sbjct: 201 YLLDRMSAWIPYVLVVASSSFIYIAVCDLMPQMKRRPRWQESAVQIALVTAGIVMIFLLT 260 Query: 239 HQAHVH 244 H H Sbjct: 261 DGLHHH 266 >YIL023c [P] COG0428 Predicted divalent heavy-metal cations transporter Sce Saccharomyces cerevisiae Length = 346 Score = 87.4 bits (215), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 59/209 (28%), Positives = 100/209 (47%), Gaps = 20/209 (9%) Query: 22 MVSLSVGIMLSTSLLHALPEAFESQAEPHTLFAVLLGGLLVFFLLEKTAILRHSHHHEDD 81 +VS S+G +L LLH +PE+ + + + G + F L+KT + S DD Sbjct: 103 LVSFSLGTLLGDILLHVIPESLSGVTDVTMVGGAIFLGFISFLTLDKTMRIL-SGTSNDD 161 Query: 82 GHG---------HHHGHDKKEAGKAGWMILVGDGLHNFTDGILIAAAFLADPHLGLVTGL 132 G K + ++ ++ H+ TDGI +A +F + +G++T + Sbjct: 162 GSIHSHSHSHTPQQTAEKKAGFNMSAYLNVISGIAHHITDGIALATSFYSSTQVGIMTSI 221 Query: 133 AIIAHEIPQEIGDFIVLLNAGFSRTRAYVYNLICSLMAVAGGVLGYFMLERG-------- 184 A+ HEIP E+GDF +LL++GF+ +A + + AV G +G +M E G Sbjct: 222 AVTFHEIPHELGDFAILLSSGFTFPQAIRAQAVTAFGAVVGTSIGCWMNEIGNNSHKATS 281 Query: 185 --MEWIPFVLVFASSGFIYIAVSDLMPQM 211 +L F + G IYIA + ++PQ+ Sbjct: 282 SSANASELMLPFTAGGLIYIATTSVVPQI 310 >SPAP8A3.03 [P] COG0428 Predicted divalent heavy-metal cations transporter Spo Schizosaccharomyces pombe Length = 453 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 41/151 (27%), Positives = 75/151 (49%), Gaps = 12/151 (7%) Query: 73 RHSHHHEDD--GHGHHHGHDKKEAGKAGWMILVGDGLHNFTDGILIAAAFLADPHLGLVT 130 ++S HE D H ++ ++ L+ D HNF DG+ I +AF + +G+ T Sbjct: 270 QNSKGHEPDLIRHVVEEVSEEYNDKTVVYLNLLCDSFHNFMDGLAITSAFFTNTSIGIST 329 Query: 131 GLAIIAHEIPQEIGDFIVLLNAGFSRTRAYVYNLICSLMAVAGGVLGYFMLERGMEWIPF 190 A++ HEIP EIGD +LL G+++++ V +I + + G ++ ++ P+ Sbjct: 330 TFAVLLHEIPAEIGDLAILLRNGYTKSQVLVLQMITMVTGLLGAIVATYIYTASSSSSPY 389 Query: 191 ----------VLVFASSGFIYIAVSDLMPQM 211 +L F + GF+YIA + P++ Sbjct: 390 GSFLLQLEDKLLPFTAGGFLYIAYLGVFPEL 420 >TM1738 [P] COG0428 Predicted divalent heavy-metal cations transporter Tma Thermotoga maritima Length = 245 Score = 63.9 bits (154), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 45/201 (22%), Positives = 91/201 (45%), Gaps = 11/201 (5%) Query: 16 SKVVERMVSLSVGIMLSTSLLHALPEAFESQAEPHTLFAVLLGGLLVFFLLEKTAILRHS 75 KV++ + + G+ML+ S + + E L +LGGL V L +K ++ H Sbjct: 33 DKVIDSFLGFAAGVMLAASAFSLVAPSLEMGGIVRFLIGFVLGGLFVN-LADK--LIPHE 89 Query: 76 HHHEDDGHGHHHGHDKKEAGKAGWMILVGDGLHNFTDGILIAAAFLADPHLGLVTGLAII 135 H + H G D K K W+ ++ +HNF +G+ + + L + +AI Sbjct: 90 HLLKG-----HEGPDTKRL-KGVWLFIIAITIHNFPEGMAVGVSAFTPQALAIA--IAIG 141 Query: 136 AHEIPQEIGDFIVLLNAGFSRTRAYVYNLICSLMAVAGGVLGYFMLERGMEWIPFVLVFA 195 IP+ L+ + + +A++ + L+ GG+LG ++ +P+++ FA Sbjct: 142 VQNIPEGAAVMASLIPMKYKKGKAFLITFLTGLVEAIGGLLGAGIVSISQRLLPYMMAFA 201 Query: 196 SSGFIYIAVSDLMPQMQRRAT 216 + IY+ +++P+ + Sbjct: 202 AGAMIYVVSDEVIPETHSKGN 222 >BB0219 Non COG Length = 273 Score = 63.5 bits (153), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 48/223 (21%), Positives = 97/223 (43%), Gaps = 24/223 (10%) Query: 16 SKVVERMVSLSVGIMLSTSLLHALPEAFESQAE------PHTLFAVLLGGLLVFFLLEKT 69 +K+++ M+ S GIM++ S + A E E +F L+G ++ + Sbjct: 44 NKIMDAMLGFSAGIMIAASFFSLIQPAIERAEELGYITWVPAVFGFLVGAFFIYIVDVFV 103 Query: 70 AILRHSHHHEDDGHGHHHGHDKKEAGKAGWMILVGDGLHNFTDGILIAAAFLA---DPHL 126 L ++D H GK +++ LHNF +G+ + AF A +P + Sbjct: 104 PDLDKLTFIDEDLTKH---------GKKDFLLFTAVTLHNFPEGLAVGVAFGALASNPDI 154 Query: 127 GLVTG-----LAIIAHEIPQEIGDFIVLLNAGFSRTRAYVYNLICSLMAVAGGVLGYFML 181 + G L I IP+ + L + + + Y + L+ + GG++G + + Sbjct: 155 QTLVGAMLLTLGIGIQNIPEGAAISLPLRRGNVALAKCFNYGQMSGLVEIVGGLMGAYAV 214 Query: 182 ERGMEWIPFVLVFASSGFIYIAVSDLMPQMQRRATLRETIPQV 224 +PF L F++ IY+++ L+P+ +R+ + +P + Sbjct: 215 YSFTRILPFALAFSAGAMIYVSIEQLIPEAKRK-DIDNKVPSI 256