BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0069.p (196 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2684 [E] COG0345 Pyrroline-5-carboxylate reductase Rso Ralsto... 201 1e-51 PA0393 [E] COG0345 Pyrroline-5-carboxylate reductase Pae Pseudom... 175 7e-44 NMB0055 [E] COG0345 Pyrroline-5-carboxylate reductase Nme Neisse... 161 1e-39 NMA0215 [E] COG0345 Pyrroline-5-carboxylate reductase NmA Neisse... 156 2e-38 HI0307 [E] COG0345 Pyrroline-5-carboxylate reductase Hin Haemoph... 142 5e-34 >RSc2684 [E] COG0345 Pyrroline-5-carboxylate reductase Rso Ralstonia solanacearum Length = 274 Score = 201 bits (510), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 104/192 (54%), Positives = 133/192 (69%) Query: 1 VKIAFIGGGNMAAALIGGLAGKVAAAEDIHVVDLNADALQALKDKFGVTTAQSIDGTLAN 60 +K FIGGGNMA+ALIGGL + A +I VVD A +G TT + LA+ Sbjct: 5 LKFGFIGGGNMASALIGGLVSRGAPGANIVVVDPTEPARAHAAQTWGATTIAAPGAELAD 64 Query: 61 ADVVVLAVKPQQMREVVASLKPFIGAQLIVSIAAGIRMVDLSRWLDGHQAIVRAMPNTPA 120 DV+VLAVKPQQMREV A L P + L++S+AAGIR+ DLSRWL GH +VRAMPNTPA Sbjct: 65 RDVIVLAVKPQQMREVCAQLAPHLRDSLVLSVAAGIRIQDLSRWLGGHARVVRAMPNTPA 124 Query: 121 LIGKGISGVVPHAQVSTLQRAQADSILKAVGETLWLDSEEKLDAVTAVSGSGPAYVFYFL 180 L G G++G+ A + R A +I AVG+++W+ E ++DAVTA+SGSGPAYVFYF+ Sbjct: 125 LSGMGMTGLAARAGLGGTDRDIASAIANAVGKSVWVPDEAQIDAVTAISGSGPAYVFYFI 184 Query: 181 EAMEQAAAQLGL 192 EAM+Q A +LGL Sbjct: 185 EAMQQVALELGL 196 >PA0393 [E] COG0345 Pyrroline-5-carboxylate reductase Pae Pseudomonas aeruginosa Length = 273 Score = 175 bits (443), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 97/194 (50%), Positives = 124/194 (63%), Gaps = 1/194 (0%) Query: 2 KIAFIGGGNMAAALIGGLAGKVAAAEDIHVVDLNADALQALKDKFGVTTAQSIDGTLANA 61 +IAFIG GNMAA+LIGGL + A I D A+ + +F + +S +A+A Sbjct: 5 RIAFIGAGNMAASLIGGLRAQGVPAAQIRASDPGAEQRAKIAGEFAIDVVESNAEAVADA 64 Query: 62 DVVVLAVKPQQMREVVASLKPFIGA-QLIVSIAAGIRMVDLSRWLDGHQAIVRAMPNTPA 120 DVVVL+VKPQ M+ V +L P + QLIVSIAAGI L WL + +VR MPNTPA Sbjct: 65 DVVVLSVKPQAMKAVCQALAPALKPEQLIVSIAAGIPCASLEAWLGQPRPVVRCMPNTPA 124 Query: 121 LIGKGISGVVPHAQVSTLQRAQADSILKAVGETLWLDSEEKLDAVTAVSGSGPAYVFYFL 180 L+ +G SG+ +AQVS Q QA +L AVG LWLD E ++DAVTAVSGSGPAY F + Sbjct: 125 LLRQGASGLYANAQVSAAQCEQAGQLLSAVGIALWLDDEAQIDAVTAVSGSGPAYFFLLM 184 Query: 181 EAMEQAAAQLGLRK 194 +AM A +LGL + Sbjct: 185 QAMTDAGEKLGLSR 198 >NMB0055 [E] COG0345 Pyrroline-5-carboxylate reductase Nme Neisseria meningitidis MC58 Length = 263 Score = 161 bits (407), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 84/192 (43%), Positives = 125/192 (65%), Gaps = 5/192 (2%) Query: 1 VKIAFIGGGNMAAALIGGLAGKVAAAEDIHVVDLNADALQALKDKFGVTTAQSIDGTLAN 60 + + F+GGGNMAAA+ GGL + I++ + A+ + L+ + GV T+ ++ L + Sbjct: 1 MNVYFLGGGNMAAAVAGGLVKQ--GGYRIYIANRGAEKRERLEKELGVETSATLP-ELHS 57 Query: 61 ADVVVLAVKPQQMREVVASLKPFIGAQLIVSIAAGIRMVDLSRWLDGHQAIVRAMPNTPA 120 DV++LAVKPQ M +++ L++S+AAG+ + LSR+L G + IVR MPNTP Sbjct: 58 DDVLILAVKPQDMEAACKNIR--TNGALVLSVAAGLSVGTLSRYLGGTRRIVRVMPNTPG 115 Query: 121 LIGKGISGVVPHAQVSTLQRAQADSILKAVGETLWLDSEEKLDAVTAVSGSGPAYVFYFL 180 IG G+SG+ A+VS R AD I+K+VG T+WLD EEK+ +T +SGSGPAYVFY L Sbjct: 116 KIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLDDEEKMHGITGISGSGPAYVFYLL 175 Query: 181 EAMEQAAAQLGL 192 +A++ AA + G Sbjct: 176 DALQNAAIRQGF 187 >NMA0215 [E] COG0345 Pyrroline-5-carboxylate reductase NmA Neisseria meningitidis Z2491 Length = 263 Score = 156 bits (395), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 82/192 (42%), Positives = 124/192 (64%), Gaps = 5/192 (2%) Query: 1 VKIAFIGGGNMAAALIGGLAGKVAAAEDIHVVDLNADALQALKDKFGVTTAQSIDGTLAN 60 + + F+GGGNMAAA+ GGL + IH+ + A+ + L + GV T+ ++ L + Sbjct: 1 MNVYFLGGGNMAAAVAGGLVKQ--GGYRIHIANRGAEKRERLGKELGVETSATLP-ELHS 57 Query: 61 ADVVVLAVKPQQMREVVASLKPFIGAQLIVSIAAGIRMVDLSRWLDGHQAIVRAMPNTPA 120 DV++LAVKPQ M +++ L++S+AAG+ + LSR+L G + IVR MPNTP Sbjct: 58 DDVLILAVKPQDMEAACKNIR--TNGALVLSVAAGLSVDTLSRYLGGTRRIVRVMPNTPG 115 Query: 121 LIGKGISGVVPHAQVSTLQRAQADSILKAVGETLWLDSEEKLDAVTAVSGSGPAYVFYFL 180 IG G+SG+ A+VS R AD I+K+VG T+WL+ E ++ ++T +SGSGPAYVFY L Sbjct: 116 KIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSITGISGSGPAYVFYLL 175 Query: 181 EAMEQAAAQLGL 192 +A++ AA + G Sbjct: 176 DALQNAAIRQGF 187 >HI0307 [E] COG0345 Pyrroline-5-carboxylate reductase Hin Haemophilus influenzae Length = 271 Score = 142 bits (358), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 84/191 (43%), Positives = 118/191 (61%), Gaps = 2/191 (1%) Query: 3 IAFIGGGNMAAALIGGLAGKVAAAEDIHVVDLNADALQALKDKFGVTTAQSIDGTLANAD 62 IAFIGGGNMA A+I GL + AE I V D N + +A V T+++ G+ A+ Sbjct: 6 IAFIGGGNMAQAIILGLLKQGYPAEQIIVNDPNEEK-RAFFANLDVATSENNVGSAIKAE 64 Query: 63 VVVLAVKPQQMREVVASLKPF-IGAQLIVSIAAGIRMVDLSRWLDGHQAIVRAMPNTPAL 121 VV+LAVKPQ M EV + L +L++SIAAGI L+ + ++IVR MPNTPAL Sbjct: 65 VVLLAVKPQMMAEVCSPLSAVDFSDKLLISIAAGISTERLNALIPSVKSIVRVMPNTPAL 124 Query: 122 IGKGISGVVPHAQVSTLQRAQADSILKAVGETLWLDSEEKLDAVTAVSGSGPAYVFYFLE 181 +G+G++G+ S R A +L AVG T+W++ E ++ AVTA SGS PAY F LE Sbjct: 125 VGEGMAGLFAPKNTSENYRTFAQDLLGAVGRTVWVNDETQMHAVTAASGSSPAYFFLMLE 184 Query: 182 AMEQAAAQLGL 192 AM++A ++ + Sbjct: 185 AMQKALIKMNI 195