BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0005.p (465 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cgl0416 [GEPR] COG0477 Permeases of the major facilitator superf... 324 2e-88 STM0212 [GEPR] COG0477 Permeases of the major facilitator superf... 241 4e-63 ECs2778 [GEPR] COG0477 Permeases of the major facilitator superf... 226 7e-59 PA3749 [GEPR] COG0477 Permeases of the major facilitator superfa... 225 2e-58 ZshiA [GEPR] COG0477 Permeases of the major facilitator superfam... 225 2e-58 >Cgl0416 [GEPR] COG0477 Permeases of the major facilitator superfamily Cgl Corynebacterium glutamicum Length = 467 Score = 324 bits (831), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 180/436 (41%), Positives = 268/436 (61%), Gaps = 7/436 (1%) Query: 4 SHS-SDHSQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFP-KGDPTTAIIASLA 61 +HS S + K+AA S WIGSALEYYDF +Y TAA+L+ LFFP P AI+A++ Sbjct: 9 THSESTPGKTPKRAALSSWIGSALEYYDFAVYGTAAALVLNHLFFPADTSPGIAILAAMG 68 Query: 62 TYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALL 121 T GV YV RP+GA ++G LGD +GRK VL+ C+FL+G ST VG LPT+DQVG APALL Sbjct: 69 TVGVAYVVRPLGALIMGPLGDRYGRKFVLMLCLFLIGASTFAVGCLPTFDQVGYLAPALL 128 Query: 122 VIMRLAQGFAVAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYM 181 V+ R+ QG + +GE S A S+ LEH+ R + +S+TL G Q G +LA VF+P ++ Sbjct: 129 VLCRVIQGLSASGEQSSAISVSLEHADERHRAFTASWTLHGTQFGTLLATGVFIPFTLFL 188 Query: 182 PAEQFNVWGWRIPFLLSFLVIIAGWIIRRTVEETPLFKAADKNELKRRSPVVEAFRDSSP 241 + WGWR+PF LS V++ ++IRR +EE P F+ + SP+ R Sbjct: 189 SEDALMSWGWRVPFWLSAAVVLVAFLIRRGLEEPPAFRENKEAVAGAASPLAMTLRYHKA 248 Query: 242 NMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGK 301 + RV A++N + +V T++ ++A +G + L +PVV N+VA++ IP G Sbjct: 249 AVARVAIAAMINSVNIVFTVWALSFATNI---VGLDRSTVLLVPVVANLVALIAIPLSGM 305 Query: 302 LSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFY 361 L+DRIGRRP I+G++ GL YL AI N + F M +LM G++Y NAV+P+FY Sbjct: 306 LADRIGRRPVFIMGAIGGGLAMNGYLGAIYSGNWTMIFFMGVLMSGLLYSMGNAVWPAFY 365 Query: 362 PELFPARSRVSAMAIAQNVGTAIT-AMLPALFTAVAPPGSSNIWLTVGSIAFGITIVSAL 420 E+FP RV+ +A+ +G A++ +P + +A+A + W+ V + ++SAL Sbjct: 366 AEMFPTSVRVTGLALGTQIGFAVSGGFVPVIASALAGD-QGDQWMKVSIFVGVVCVISAL 424 Query: 421 AALSARETYRIPLEKL 436 A++A+ET + L+++ Sbjct: 425 VAMTAKETKALTLDEI 440 >STM0212 [GEPR] COG0477 Permeases of the major facilitator superfamily Sty Salmonella typhimurium LT2 Length = 433 Score = 241 bits (614), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 149/430 (34%), Positives = 229/430 (53%), Gaps = 11/430 (2%) Query: 6 SSDHSQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGV 65 S ++ ++ S WIG+++EYYDF +Y A++L+F LFFP P TA ++S A++GV Sbjct: 2 SGTYNATIRRVVVSAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGV 61 Query: 66 GYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMR 125 G+++RP+GA G+ GDT GRK LL + MG T +G LP+Y +G APALLVI+R Sbjct: 62 GFISRPLGALFFGNRGDTLGRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILR 121 Query: 126 LAQGFAVAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYMPAEQ 185 QGF V GE GA M++E++ RG S+ + G GQ+LA VF+ + +P E Sbjct: 122 FLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLTGQLLATGVFIVVTQ-LPQEA 180 Query: 186 FNVWGWRIPFLLSFLVIIAGWIIRRTVEETPLFKAADK----NELKRRS--PVVEAFRDS 239 WGWRIPFLLS L+++ G +R ++ETP+F+A K N ++R PVV+ R+ Sbjct: 181 LLSWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAINHRQQREERPVVKVVREQ 240 Query: 240 SPNMLRVVCMALMNVIP-VVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPY 298 ++L ++ + +P +AT+F ++A +G A L+I + ++A + Sbjct: 241 WRSILLIIILRFAESVPFFLATVFAVSWATTQ---LGIASLTILYIVMFTCLLAYPMHVL 297 Query: 299 VGKLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFP 358 G +SDR G R I G+L AF + + + R++ L + +L+ + + NAV P Sbjct: 298 FGIMSDRRGCRQVYIFGALFVAAMAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQP 357 Query: 359 SFYPELFPARSRVSAMAIAQNVGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFGITIVS 418 SF+ LF R S +I +G + N W V + S Sbjct: 358 SFFAGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDNSWALVAGYVVLTALAS 417 Query: 419 ALAALSARET 428 A AA A ET Sbjct: 418 AFAAKIAPET 427 >ECs2778 [GEPR] COG0477 Permeases of the major facilitator superfamily Ecs Escherichia coli O157:H7 Length = 438 Score = 226 bits (577), Expect = 7e-59, Method: Compositional matrix adjust. Identities = 127/423 (30%), Positives = 226/423 (53%), Gaps = 6/423 (1%) Query: 11 QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVAR 70 ++++AA + G+ +++YDF +Y A+L+F + FFP+ P +A+ AT+GVG++ R Sbjct: 18 SRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFATFGVGFLFR 77 Query: 71 PIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQGF 130 P+G + GH GD GRK++L+ +++MG +T +G+LP++ +G WAP LLV +R QGF Sbjct: 78 PLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLVTLRAIQGF 137 Query: 131 AVAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVWG 190 AV GE GA+ + +E +P ++ +YSS G G +L+ + ++ EQF WG Sbjct: 138 AVGGEWGGAALLSVESAPKNKKAFYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWG 197 Query: 191 WRIPFLLSFLVIIAGWIIRRTVEETPLFKAADKNE--LKRRSPVVEAFRDSSPNMLRVVC 248 WRIPFL S ++++ +R +EE+ F+ N+ K+R PV+EA L+++ Sbjct: 198 WRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIA 257 Query: 249 MALMNVIPV-VATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIG 307 + L ++ + + T F Y+ Q +G ++++L I ++ ++ L IP L+DR G Sbjct: 258 LRLCELLTMYIVTAFALNYSTQ---NMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFG 314 Query: 308 RRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFYPELFPA 367 RR I G+L+ L AF + A+ +++ SI++ + + V + E+F A Sbjct: 315 RRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGA 374 Query: 368 RSRVSAMAIAQNVGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFGITIVSALAALSARE 427 R S + V + + A + W +V ++SA+ AL ++ Sbjct: 375 SYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD 434 Query: 428 TYR 430 R Sbjct: 435 NQR 437 >PA3749 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 437 Score = 225 bits (573), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 130/378 (34%), Positives = 212/378 (56%), Gaps = 11/378 (2%) Query: 8 DHSQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGY 67 + + + + A + +IG+A+E+YDF++YATAA+L+ +FFP+ T ++++ T+G+ + Sbjct: 13 EPANSTTRVAVASFIGTAIEFYDFYVYATAAALVIGPVFFPQTSGTAQMLSAFLTFGIAF 72 Query: 68 VARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLA 127 +ARP+G+ + GH GD GRK L+ + LMG ST +G+LP YD +G WAP LL ++R Sbjct: 73 LARPLGSALFGHFGDRIGRKSTLVASLLLMGVSTTLIGVLPGYDSIGYWAPLLLCVLRFG 132 Query: 128 QGFAVAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFN 187 QG + GE GA+ + E++P G+R ++ F G G + A +FL LA + EQF Sbjct: 133 QGLGLGGEWGGAALLATENAPAGKRAWFGMFPQLGPSIGFLAANGLFLALAMLLSEEQFR 192 Query: 188 VWGWRIPFLLSFLVIIAGWIIRRTVEETPLFKAADKNELKRRSPVVEAFRDS-SPNMLRV 246 WGWRIPFLLS +++ G +R + ETP+F A + R P+ E F P +L Sbjct: 193 EWGWRIPFLLSAALVVVGLYVRLKLAETPVFAKAMAKHERVRLPIAELFAQHWRPTLLGA 252 Query: 247 VCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRI 306 + M + + ++T+F +Y V +GF+++ +L + + + P LSDR Sbjct: 253 LAMVVCYALFYISTVFSLSYGVA---SLGFSREEFLGLLCLAVLFMAAATPLSAWLSDRF 309 Query: 307 GRRPPIIVGSLLAGLFAFA---YLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFYPE 363 GR+P +++GSL A FA L S +V L + + + GV + A+ PE Sbjct: 310 GRKPVLLLGSLAAIASGFAMEPLLSQGSTFSVALFLCIELFLMGVTFAPMGALL----PE 365 Query: 364 LFPARSRVSAMAIAQNVG 381 +FP R + + A N+G Sbjct: 366 IFPTHVRYTGASAAYNLG 383 >ZshiA [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 438 Score = 225 bits (573), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 126/423 (29%), Positives = 225/423 (53%), Gaps = 6/423 (1%) Query: 11 QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVAR 70 ++++AA + G+ +++YDF +Y A+L+F + FFP+ P +A+ AT+GVG++ R Sbjct: 18 SRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFATFGVGFLFR 77 Query: 71 PIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQGF 130 P+G + GH GD GRK++L+ +++MG +T +G+LP++ +G WAP LLV +R QGF Sbjct: 78 PLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLVTLRAIQGF 137 Query: 131 AVAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVWG 190 AV GE GA+ + +E +P ++ +YSS G G +L+ + ++ EQF WG Sbjct: 138 AVGGEWGGAALLSVESAPKNKKAFYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWG 197 Query: 191 WRIPFLLSFLVIIAGWIIRRTVEETPLFKAADKNE--LKRRSPVVEAFRDSSPNMLRVVC 248 WRIPFL S ++++ +R +EE+ F+ N+ K+R PV+EA L+++ Sbjct: 198 WRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIA 257 Query: 249 MALMNVIPV-VATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIG 307 + L ++ + + T F Y+ Q +G ++++L I ++ ++ L IP +DR G Sbjct: 258 LRLCELLTMYIVTAFALNYSTQ---NMGLPRELFLNIGLLVGGLSCLTIPCFAWXADRFG 314 Query: 308 RRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFYPELFPA 367 RR I G+L+ L AF + A+ +++ SI++ + + V + E+F A Sbjct: 315 RRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGA 374 Query: 368 RSRVSAMAIAQNVGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFGITIVSALAALSARE 427 R S + V + + A + W +V ++SA+ AL ++ Sbjct: 375 SYRYSGAGVGYQVASVVXGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD 434 Query: 428 TYR 430 R Sbjct: 435 NQR 437