BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0178.p (235 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Ta0124m [R] COG2872 Predicted metal-dependent hydrolases related... 79 1e-14 PH0108 [R] COG2872 Predicted metal-dependent hydrolases related ... 77 3e-14 PAB0066 [R] COG2872 Predicted metal-dependent hydrolases related... 77 4e-14 SSO0450 [R] COG2872 Predicted metal-dependent hydrolases related... 73 5e-13 MA2014 [R] COG2872 Predicted metal-dependent hydrolases related ... 73 6e-13 >Ta0124m [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Tac Thermoplasma acidophilum Length = 241 Score = 78.6 bits (192), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 54/191 (28%), Positives = 86/191 (45%), Gaps = 9/191 (4%) Query: 9 MTHKLFWSAPYQTELDTTVTAVDGDQVQIAQTIFFAFSGGQESDAGTLGG----HPVLQA 64 MT KL++ Y E V +VD + + + +T F+ GGQ +D G + + V+ Sbjct: 1 MTEKLYYDDMYMKEFQAEVVSVDSNGIVLDRTAFYPTGGGQPNDTGIIVNDDKTYRVVDV 60 Query: 65 EKRDQTIVYTLPPGHGLRVGDAVYLQIDWARRYRLMRLHFAAEMVLQLVYQMRPGIERI- 123 +K +V+ L GL+ GD +Y +IDW RRY MR H A +L + + E + Sbjct: 61 QKHGDDVVHILESVDGLKAGDRIYGKIDWERRYAHMRYHTAIH-ILDGIVSAKHNSEALL 119 Query: 124 -GAHIAMDKARVDFASDTSLAPLFPAIEAAAQELVAAGLPIVTA-FSDEAAGRIPS-DRA 180 G I D++R+D + I A E ++ G + S E A + P+ R Sbjct: 120 TGGQIYEDRSRIDMNIENFTKEFVDQIIQEANEFISEGHRVYAENISREEAMKRPNLART 179 Query: 181 WPAVARIRAPP 191 P I + P Sbjct: 180 EPGRKLIESLP 190 >PH0108 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Pho Pyrococcus horikoshii Length = 216 Score = 77.4 bits (189), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 45/156 (28%), Positives = 78/156 (50%), Gaps = 3/156 (1%) Query: 8 NMTHKLFWSAPYQTELDTTVTAVDGDQVQIAQTIFFAFSGGQESDAGTLGGHPVLQAEKR 67 NMT KL++ Y E V + + + + QTIF+ GGQ D GT+ G VL K Sbjct: 3 NMTRKLYYEDAYLKEAKGRVLEIRDNAILLDQTIFYPTGGGQPHDRGTINGVEVLDVYKD 62 Query: 68 DQ-TIVYTLPPGHGLRVGDAVYLQIDWARRYRLMRLHFAAEMVLQLVYQM--RPGIERIG 124 ++ + + + +VGD V L+IDW RY+LMR+H ++ ++ ++ + +G Sbjct: 63 EEGNVWHVVKEPEKFKVGDEVELKIDWDYRYKLMRIHTGLHLLEHVLNEVLGEGNWQLVG 122 Query: 125 AHIAMDKARVDFASDTSLAPLFPAIEAAAQELVAAG 160 + ++++K R D A +L I + + V G Sbjct: 123 SGMSVEKGRYDIAYPENLNKYKEQIISLFNKYVDEG 158 >PAB0066 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Pab Pyrococcus abyssi Length = 213 Score = 76.6 bits (187), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 46/166 (27%), Positives = 80/166 (48%), Gaps = 3/166 (1%) Query: 9 MTHKLFWSAPYQTELDTTVTAVDGDQVQIAQTIFFAFSGGQESDAGTLGGHPVLQAEKRD 68 MT KL++ Y E V + + + + QTIF+ GGQ D GT+ G VL K + Sbjct: 1 MTRKLYYEDAYLKEAKAKVVEIKDNALLLDQTIFYPTGGGQPHDRGTINGVEVLDVYKDE 60 Query: 69 Q-TIVYTLPPGHGLRVGDAVYLQIDWARRYRLMRLHFAAEMVLQLVYQM--RPGIERIGA 125 + + + + +VGD V L+IDW RY+LMR+H ++ ++ ++ E +G+ Sbjct: 61 EGNVWHVVKEPEKFKVGDEVELKIDWDYRYKLMRIHTGLHLLEHVLNEVLGEGKWELVGS 120 Query: 126 HIAMDKARVDFASDTSLAPLFPAIEAAAQELVAAGLPIVTAFSDEA 171 ++++K R D A +L I + + V G + + E Sbjct: 121 GMSVEKGRYDIAYPENLNKYKEQIISLFNKYVDEGGEVKIWWEGET 166 >SSO0450 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Sso Sulfolobus solfataricus Length = 236 Score = 72.8 bits (177), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 58/200 (29%), Positives = 95/200 (47%), Gaps = 26/200 (13%) Query: 9 MTHKLFWSAPYQTELDTTVTAVDGDQVQIAQTIFFAFSGGQESDAGTL----GGHPVLQA 64 MT +L+ Y E + V ++G+ V + +T F+ GG ++D G L G + Sbjct: 1 MTEELYLKDSYIKEFEGRVVRIEGNYVILDKTAFYPGGGGLDNDTGFLVNEKGERISVTE 60 Query: 65 EKRDQT--IVYTLPPGHGLRVGDAVYLQIDWARRYRLMRLHFAAEMVLQLVYQMRPGIER 122 KR + I++ + L V + V IDW RRYR+MRLH A+ +V L Y+ + G Sbjct: 61 VKRGENGEILHKIDQNGSLNVNEKVIGTIDWDRRYRMMRLHTASHIVAALAYR-KFGALI 119 Query: 123 IGAHIAMDKARVDFASDT--SLAPLFPAIEAAAQELVAAGLPIVTAFSDEAAGRIPSDRA 180 G HI+ ++A+ DF + +L L A E++ + + F +P + A Sbjct: 120 TGGHISPEQAKDDFNVENKDTLIELI----NEANEIIKKDIELKIYF-------LPREEA 168 Query: 181 W--PAVARI--RAPP--PKW 194 PA+ ++ R PP P W Sbjct: 169 LMIPAIVKLAGRNPPQIPIW 188 >MA2014 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Mac Methanosarcina acetivorans str.C2A Length = 245 Score = 72.8 bits (177), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 52/167 (31%), Positives = 81/167 (48%), Gaps = 13/167 (7%) Query: 9 MTHKLFWSAPYQTELDTTVTAVDGDQ-VQIAQTIFFAFSGGQESDAGTL------GGHPV 61 MT L++ Y E + V V D+ V + +T F+ SGGQ SD G L V Sbjct: 1 MTEVLYFLDCYLREFEAIVEKVTDDKYVVLDRTAFYPESGGQPSDTGKLVREEDGAEFEV 60 Query: 62 LQAEKRDQTIVYTLPP--GH---GLRVGDAVYLQIDWARRYRLMRLHFAAEMVLQLVYQM 116 + K + I + + P G+ GL+ GD V IDW RRYR MR+H A ++ ++ + Sbjct: 61 VYVGKFNGDISHEITPVDGNAALGLKAGDRVRGIIDWDRRYRHMRMHTATHVIANVI-EK 119 Query: 117 RPGIERIGAHIAMDKARVDFASDTSLAPLFPAIEAAAQELVAAGLPI 163 G + G + +DK+RVDF+ + F E A +++ P+ Sbjct: 120 EAGAQITGNQLGLDKSRVDFSLEAFDRDKFAEYEKIANKVITENHPV 166