BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0161.p (360 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc3025 [L] COG0514 Superfamily II DNA helicase Rso Ralstonia so... 483 e-136 DR1289 [L] COG0514 Superfamily II DNA helicase Dra Deinococcus r... 475 e-134 PA3344 [L] COG0514 Superfamily II DNA helicase Pae Pseudomonas a... 442 e-124 XF1381 [L] COG0514 Superfamily II DNA helicase Xfa Xylella fasti... 438 e-123 NMB0274 [L] COG0514 Superfamily II DNA helicase Nme Neisseria me... 424 e-118 >RSc3025 [L] COG0514 Superfamily II DNA helicase Rso Ralstonia solanacearum Length = 637 Score = 483 bits (1242), Expect = e-136, Method: Compositional matrix adjust. Identities = 246/344 (71%), Positives = 276/344 (80%), Gaps = 10/344 (2%) Query: 4 FDSLPPGATQQERALHVLETVFGYSSFRGHQGEIVQHVAGGGDALVLMPTGGGKSLCYQV 63 D+LP RA VL VFGYS+FRG Q +IV HVA GGD LVLMPTGGGKSLCYQ+ Sbjct: 11 LDALPEDTAAATRA--VLHDVFGYSAFRGPQADIVAHVADGGDCLVLMPTGGGKSLCYQI 68 Query: 64 PALLR----EGTGVVVSPLIALMQDQVDALAEVGVRAAFLNSTQSFDEALQIERRLRQGD 119 PAL+R +G G+VVSPLIALMQDQV AL E GVRAA+LNS S EA Q+ER L G Sbjct: 69 PALVRHRRGQGAGIVVSPLIALMQDQVAALEEAGVRAAYLNSALSGAEAAQVERDLAAGR 128 Query: 120 LDLLYVAPERLMTPRCLDLLESARISLFAIDEAHCVSQWGHDFRPEYIKLSILHERFPQV 179 LDL+YVAPERLMTPR L+LLE +RI LFAIDEAHCVSQWGHDFRPEYI+LS+LHERFP V Sbjct: 129 LDLVYVAPERLMTPRFLELLERSRIGLFAIDEAHCVSQWGHDFRPEYIQLSVLHERFPHV 188 Query: 180 PRIALTATADQQTREEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKNEH 239 PRIALTATAD TR+EI+ RL L AR F+SSFDRPNIRY IVEK + R+QLL FI+ EH Sbjct: 189 PRIALTATADAVTRDEIVERLALGGARVFLSSFDRPNIRYTIVEKDSARQQLLRFIRAEH 248 Query: 240 PE----DAGIVYCLSRKKVEETAEFLRGEGLNALAYHAGMDYALRTKNQARFLREDKIVM 295 DAGI+YCLSRKKVEETA++L +GL AL YHAGMD R ++QA F +E+ +VM Sbjct: 249 LGEDGCDAGIIYCLSRKKVEETAQWLAEQGLRALPYHAGMDAETRARHQAIFRKEEGVVM 308 Query: 296 VATIAFGMGIDKPDVRFVCHLDLPKSVEGYYQETGRAGRDGLPA 339 VATIAFGMGIDKPDVRFV HLDLPKS+EGYYQETGRAGRDGLPA Sbjct: 309 VATIAFGMGIDKPDVRFVAHLDLPKSLEGYYQETGRAGRDGLPA 352 >DR1289 [L] COG0514 Superfamily II DNA helicase Dra Deinococcus radiodurans Length = 824 Score = 475 bits (1223), Expect = e-134, Method: Compositional matrix adjust. Identities = 229/329 (69%), Positives = 277/329 (84%) Query: 11 ATQQERALHVLETVFGYSSFRGHQGEIVQHVAGGGDALVLMPTGGGKSLCYQVPALLREG 70 A +RALH+L+T++GY +FRG QGEIVQ VA GG+ALVLMPTGGGKSLCYQ+P+LLR G Sbjct: 6 AALHDRALHLLQTIWGYPAFRGVQGEIVQQVAEGGNALVLMPTGGGKSLCYQLPSLLRPG 65 Query: 71 TGVVVSPLIALMQDQVDALAEVGVRAAFLNSTQSFDEALQIERRLRQGDLDLLYVAPERL 130 TG+VVSPLIALM+DQVD L + GVRAAFLNST EA ++E L +GDLDLLYVAPERL Sbjct: 66 TGIVVSPLIALMKDQVDTLRQNGVRAAFLNSTLLPHEAREVEDALLRGDLDLLYVAPERL 125 Query: 131 MTPRCLDLLESARISLFAIDEAHCVSQWGHDFRPEYIKLSILHERFPQVPRIALTATADQ 190 + PR LDLLE A ++LFAIDEAHCVSQWGHDFRPEY +LS+L ERFP++PR+ALTATAD+ Sbjct: 126 LMPRTLDLLERAPVALFAIDEAHCVSQWGHDFRPEYQQLSVLAERFPELPRVALTATADE 185 Query: 191 QTREEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKNEHPEDAGIVYCLS 250 +TR +I L+LEDA QFVSSFDRPNI+Y++ K + + QLL FI+ EHP DAGIVYCLS Sbjct: 186 RTRADIKSVLRLEDAPQFVSSFDRPNIQYRVGLKDSPKTQLLHFIREEHPGDAGIVYCLS 245 Query: 251 RKKVEETAEFLRGEGLNALAYHAGMDYALRTKNQARFLREDKIVMVATIAFGMGIDKPDV 310 RK VEETA++L+ +G++ALAYHAG+ R Q RFL E+ +++ AT+AFGMGIDKP+V Sbjct: 246 RKSVEETAKWLQAQGIDALAYHAGLSSTERNNVQERFLNEEGVIVCATVAFGMGIDKPNV 305 Query: 311 RFVCHLDLPKSVEGYYQETGRAGRDGLPA 339 RFV HLDLPKS+EGYYQETGRAGRDGLP+ Sbjct: 306 RFVAHLDLPKSMEGYYQETGRAGRDGLPS 334 >PA3344 [L] COG0514 Superfamily II DNA helicase Pae Pseudomonas aeruginosa Length = 712 Score = 442 bits (1136), Expect = e-124, Method: Compositional matrix adjust. Identities = 221/326 (67%), Positives = 261/326 (80%), Gaps = 1/326 (0%) Query: 14 QERALHVLETVFGYSSFRGHQGEIVQHVAGGGDALVLMPTGGGKSLCYQVPALLREGTGV 73 +E+AL +L+ VFGY +FRG+Q I++ VA GGDALVLMPTGGGKSLC+QVPALLREG V Sbjct: 2 REQALRILKDVFGYDAFRGNQARIIERVAEGGDALVLMPTGGGKSLCFQVPALLREGLTV 61 Query: 74 VVSPLIALMQDQVDALAEVGVRAAFLNSTQSFDEALQIERRLRQGDLDLLYVAPERLMTP 133 VVSPLIALM+DQV L E+GV A LNST + ++ I RL++G++ LLY+APERL+ P Sbjct: 62 VVSPLIALMEDQVATLDELGVPAVALNSTLNPEQQRDIAERLQRGEIKLLYLAPERLVQP 121 Query: 134 RCLDLLESARISLFAIDEAHCVSQWGHDFRPEYIKLSILHERFPQVPRIALTATADQQTR 193 R L L+ + LFAIDEAHCVSQWGHDFRPEY++L L E FPQVPRIALTATAD +TR Sbjct: 122 RMLAFLQRLPVGLFAIDEAHCVSQWGHDFRPEYLQLGQLAELFPQVPRIALTATADMRTR 181 Query: 194 EEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKNEHPEDAGIVYCLSRKK 253 EE+I RL L++A QF+SSFDRPNI Y+IV K RKQLL F+ +E DAGIVYCLSRKK Sbjct: 182 EEMIQRLHLQNAEQFLSSFDRPNIFYRIVPKEQPRKQLLGFL-SERRGDAGIVYCLSRKK 240 Query: 254 VEETAEFLRGEGLNALAYHAGMDYALRTKNQARFLREDKIVMVATIAFGMGIDKPDVRFV 313 VEE AEFL +G AL YHAG+ LR +Q RFL E+ ++MVATIAFGMGIDKP+VRFV Sbjct: 241 VEEVAEFLGNQGFPALPYHAGLSNELRAHHQKRFLNEEGLIMVATIAFGMGIDKPNVRFV 300 Query: 314 CHLDLPKSVEGYYQETGRAGRDGLPA 339 HLDLPKS+E YYQETGRAGRDGLPA Sbjct: 301 AHLDLPKSLEAYYQETGRAGRDGLPA 326 >XF1381 [L] COG0514 Superfamily II DNA helicase Xfa Xylella fastidiosa 9a5c Length = 645 Score = 438 bits (1127), Expect = e-123, Method: Compositional matrix adjust. Identities = 215/317 (67%), Positives = 253/317 (79%), Gaps = 1/317 (0%) Query: 20 VLETVFGYSSFRGHQGEIVQHVAGGGDALVLMPTGGGKSLCYQVPALLREGTGVVVSPLI 79 VL+ VFGY +FRG Q I++HVA G DALVLMPTGGGKSLCYQVPALLR+G GVV+SPLI Sbjct: 55 VLQRVFGYDTFRGPQQAIIEHVAAGHDALVLMPTGGGKSLCYQVPALLRKGIGVVISPLI 114 Query: 80 ALMQDQVDALAEVGVRAAFLNSTQSFDEALQIERRLRQGDLDLLYVAPERLMTPRCLDLL 139 ALMQDQV+ L ++GVRAA+LNST +A +IE L DLDLLY+APERL+T L LL Sbjct: 115 ALMQDQVETLRQLGVRAAYLNSTLDATQAQRIEHALVTADLDLLYIAPERLLTAHFLSLL 174 Query: 140 ESARISLFAIDEAHCVSQWGHDFRPEYIKLSILHERFPQVPRIALTATADQQTREEIIHR 199 E ++I+LFAIDEAHCVSQWGHDFRPEY +L++LHER+P VPRIALTATAD T+ EI R Sbjct: 175 ERSQIALFAIDEAHCVSQWGHDFRPEYRQLTVLHERWPHVPRIALTATADPPTQREIAER 234 Query: 200 LQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKNEHPEDAGIVYCLSRKKVEETAE 259 L L DA F+SSFDRPNIRY +V+K N R+QL +F+ H AGIVY +SR+K EE A Sbjct: 235 LDLSDAHHFISSFDRPNIRYTVVQKNNMRRQLQEFL-GRHRNTAGIVYAMSRRKTEEIAA 293 Query: 260 FLRGEGLNALAYHAGMDYALRTKNQARFLREDKIVMVATIAFGMGIDKPDVRFVCHLDLP 319 +L +G NAL YHAG+ R K+Q FLRE+ IVM ATIAFGMGI+KPDVRFV H+DLP Sbjct: 294 YLCTQGYNALPYHAGLPVETRAKHQRCFLREEGIVMCATIAFGMGINKPDVRFVAHIDLP 353 Query: 320 KSVEGYYQETGRAGRDG 336 KS+EGYYQETGRAGRDG Sbjct: 354 KSLEGYYQETGRAGRDG 370 >NMB0274 [L] COG0514 Superfamily II DNA helicase Nme Neisseria meningitidis MC58 Length = 766 Score = 424 bits (1091), Expect = e-118, Method: Compositional matrix adjust. Identities = 217/334 (64%), Positives = 254/334 (76%), Gaps = 2/334 (0%) Query: 17 ALHVLETVFGYSSFRGHQGEIVQHVAGGGDALVLMPTGGGKSLCYQVPALLREGTGVVVS 76 A +L VFGY FRG Q ++ +AGGG VLMPTGGGKSLCYQ+PAL+REG VVVS Sbjct: 6 AKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGGGKSLCYQIPALMREGVAVVVS 65 Query: 77 PLIALMQDQVDALAEVGVRAAFLNSTQSFDEALQIERRLRQGDLDLLYVAPERLMTPRCL 136 PLIALM DQV L G+ AA +NS S DEA +I RL QG L LLYVAPERL+T R L Sbjct: 66 PLIALMNDQVANLHAAGIEAAAVNSGTSADEAREIADRLAQGRLKLLYVAPERLVTDRFL 125 Query: 137 DLLESARISLFAIDEAHCVSQWGHDFRPEYIKLSILHERFPQVPRIALTATADQQTREEI 196 L+ +SLFAIDEAHCVSQWGHDFRPEY +L +L ER+P VPRIALTATAD TR +I Sbjct: 126 RFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGMLAERYPNVPRIALTATADAATRADI 185 Query: 197 IHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKNEHPEDAGIVYCLSRKKVEE 256 H L L+DA +FVSSFDRPNI YQ++EK NG+KQLLDFI+ E +GIVYCLSRKKVE+ Sbjct: 186 KHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKKQLLDFIRKEMTGQSGIVYCLSRKKVED 245 Query: 257 TAEFLRGEGLNALAYHAGMDYALRTKNQARFLREDKIVMVATIAFGMGIDKPDVRFVCHL 316 A+FLR GLNA+ YHAG+ +R +NQ RF ED I++VAT+AFGMGIDKPDVRFV HL Sbjct: 246 VAQFLRENGLNAIPYHAGLSMDVREENQRRFTHEDNIIVVATVAFGMGIDKPDVRFVAHL 305 Query: 317 DLPKSVEGYYQETGRAGRDGLPA--FXCRPTPGW 348 D+P+SVE +YQE+GRAGRDGLPA + C W Sbjct: 306 DMPQSVEHFYQESGRAGRDGLPAASWLCYGLNDW 339