BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0081.p (206 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20005 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium... 263 3e-70 PA3035 [O] COG0625 Glutathione S-transferase Pae Pseudomonas aer... 171 1e-42 SMa2115 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium ... 115 7e-26 SMa1497 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium ... 109 3e-24 mll2536 [O] COG0625 Glutathione S-transferase Mlo Mesorhizobium ... 89 5e-18 >SMb20005 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium meliloti Length = 203 Score = 263 bits (671), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 134/203 (66%), Positives = 154/203 (75%) Query: 1 MKIHHFSLSGHAHRAVLFASLLGLEAELIHVDLAAGAHKQPAFLTLNPFGQVPVLEDGDI 60 MK++H LSGHAHRA LF SLLG+E EL+ VD A HKQ FL LN FGQVPVL+D Sbjct: 1 MKLYHHPLSGHAHRARLFLSLLGIEHELVVVDFARREHKQADFLKLNSFGQVPVLDDAGT 60 Query: 61 VIADSNAILVYLAKKAGRTDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLITVFGARF 120 +I+DSNAILVYLAKK GR DWLPEDA GAAAVQRWLSVAAG++A+GPA ARLI VF A + Sbjct: 61 IISDSNAILVYLAKKTGRPDWLPEDAVGAAAVQRWLSVAAGQIAHGPAQARLINVFKAPY 120 Query: 121 NPEEVIQRAHLLLGRMEQHLANRAWLAADHPTIADVAVYSYLVAAPEGNVDLSGYPSVLR 180 PEEVI R+H +L +EQ L R W+AAD PTIADVA+YSY+ APEG+VDL YP + Sbjct: 121 RPEEVIPRSHAILALIEQELEGRGWIAADRPTIADVALYSYVARAPEGDVDLQPYPEIRA 180 Query: 181 LLQGIEALPGFVPFPRTAAGLRA 203 L IEALPGFV F +T GL A Sbjct: 181 WLARIEALPGFVEFEKTPVGLAA 203 >PA3035 [O] COG0625 Glutathione S-transferase Pae Pseudomonas aeruginosa Length = 198 Score = 171 bits (433), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 91/196 (46%), Positives = 124/196 (63%), Gaps = 1/196 (0%) Query: 1 MKIHHFSLSGHAHRAVLFASLLGLEAELIHVDLAAGAHKQPAFLTLNPFGQVPVLEDGDI 60 MK+H +LSG+A++ LF SL+G EA L VD HK AFL +NP GQ+PVLEDG++ Sbjct: 1 MKLHDLALSGNAYKVRLFLSLIGREATLRPVDFLNREHKTQAFLRMNPRGQIPVLEDGEL 60 Query: 61 VIADSNAILVYLAKKAGRTDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLITVFGARF 120 + DS+AILVYLA++ W P D + WLS +A E+ GPA+ARLIT F Sbjct: 61 QLGDSHAILVYLARRHADPVWYPLDPVSQGRIAYWLSYSANEVQNGPASARLITRFNLPL 120 Query: 121 NPEEVIQRAHLLLGRMEQHLANRAWLA-ADHPTIADVAVYSYLVAAPEGNVDLSGYPSVL 179 + E+ QR +L +++HLA WLA + P+IADVA+Y YL APEG + L YP++ Sbjct: 121 DREQTHQRGLEVLRLVDRHLAEHRWLAQGEQPSIADVALYPYLALAPEGGLALDHYPALN 180 Query: 180 RLLQGIEALPGFVPFP 195 + I ALPG++P P Sbjct: 181 AWIARIRALPGYIPMP 196 >SMa2115 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium meliloti Length = 215 Score = 115 bits (288), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 67/201 (33%), Positives = 109/201 (54%), Gaps = 10/201 (4%) Query: 1 MKIHHFSLSGHAHRAVLFASLLGLEAELIHVDLAAG-AHKQPAFLTLNPFGQVPVLEDGD 59 +K++ + LSG+ ++ L S+LG++ + + VD G HK FL NP GQ+PV++D Sbjct: 2 IKLYDYELSGNCYKLRLLMSILGVDYKTVPVDFYPGREHKSEWFLKFNPLGQLPVIDDDG 61 Query: 60 IVIADSNAILVYLAKKAGRT-DWLPEDA-QGAAAVQRWLSVAAGELAYGPAAARLITVFG 117 +V+ D+ AILVYLA K W P D + + +WL+ A + +AARL Sbjct: 62 LVLRDAQAILVYLAAKYDTAGSWYPRDNPELLGEISQWLAF-ADSITSTASAARLHDALF 120 Query: 118 ARFNPEEVIQRAHLLLGRMEQHL-----ANRAWL-AADHPTIADVAVYSYLVAAPEGNVD 171 F+ E AH L +++HL R W+ +A HPTIAD+A + Y++ + EG + Sbjct: 121 YDFDIETARAGAHRLFRILDEHLWFGERQERQWICSAKHPTIADIACFPYIMLSEEGGIS 180 Query: 172 LSGYPSVLRLLQGIEALPGFV 192 YP++ R ++ + GF+ Sbjct: 181 RQDYPAIRRWCDRVKRINGFI 201 >SMa1497 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium meliloti Length = 215 Score = 109 bits (273), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 66/201 (32%), Positives = 107/201 (53%), Gaps = 10/201 (4%) Query: 1 MKIHHFSLSGHAHRAVLFASLLGLEAELIHVDLAAG-AHKQPAFLTLNPFGQVPVLEDGD 59 + ++ + LSG+ ++ L S+LG+E + + VD G HK FL LNP GQ+PV+ED Sbjct: 2 ITLYDYELSGNCYKLRLLMSMLGIEYKTVPVDFYPGREHKSDWFLKLNPLGQLPVIEDDG 61 Query: 60 IVIADSNAILVYLAKKAGRT-DWLPEDAQG-AAAVQRWLSVAAGELAYGPAAARLITVFG 117 +V+ D+ AILVY+ K + W P D + +WL+ A G + +AARL Sbjct: 62 LVLRDAQAILVYIGSKYDPSGTWYPRDNPALLGEISQWLAFADG-ITGTASAARLHDGLF 120 Query: 118 ARFNPEEVIQRAHLLLGRMEQHL-----ANRAWL-AADHPTIADVAVYSYLVAAPEGNVD 171 + E AH L +++HL R W+ +A HPT+AD+A + Y++ + EG + Sbjct: 121 YELDVEAARAGAHRLFRILDEHLWFGEQEGRDWICSAPHPTVADIACFPYIMLSEEGGIP 180 Query: 172 LSGYPSVLRLLQGIEALPGFV 192 YP++ R ++ + GF Sbjct: 181 RQDYPAIRRWCDRLKRIKGFT 201 >mll2536 [O] COG0625 Glutathione S-transferase Mlo Mesorhizobium loti Length = 202 Score = 89.4 bits (220), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 66/202 (32%), Positives = 103/202 (50%), Gaps = 14/202 (6%) Query: 1 MKIHHFSLSGHAHRAVLFASLLGLEAELIHVDLAAGAHKQPAFLTLNPFGQVPVLE-DGD 59 M ++ SG+ ++ L + LGL I V G ++ F+ NP VP+LE D Sbjct: 1 MILYSMIDSGNCYKPRLLMAKLGLAFTTIEVSSHTGETRKADFVAKNPNAMVPLLELDDG 60 Query: 60 IVIADSNAILVYLAKKAGRTDWLPEDAQGAAAVQRWLSVAAGELAYGP--AAARLITVFG 117 +A+SNAIL+YLA+ T +LP D +WL + ++ P A + + F Sbjct: 61 RRLAESNAILLYLAEG---TRFLPADKYERGLAYQWLFFE--QYSHEPYIAVRKALLTFP 115 Query: 118 ARF---NPEEV---IQRAHLLLGRMEQHLANRAWLAADHPTIADVAVYSYLVAAPEGNVD 171 R PE + ++R H LG M ++L N A+ A + ++AD+A+Y+Y A +G Sbjct: 116 ERARDATPERLAMTLERGHKALGVMNRYLENNAFFAGNAFSVADIALYAYTHTAEQGGFQ 175 Query: 172 LSGYPSVLRLLQGIEALPGFVP 193 L YP+V+ L +EA PG VP Sbjct: 176 LDAYPAVVAWLGRVEADPGHVP 197