BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0061.p (286 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc01175 [IQR] COG1028 Dehydrogenases with different specificiti... 146 6e-35 mll0352 [IQR] COG1028 Dehydrogenases with different specificitie... 113 4e-25 FN0216 [IQR] COG1028 Dehydrogenases with different specificities... 112 1e-24 VC1119 [IQR] COG1028 Dehydrogenases with different specificities... 106 5e-23 BS_yqjQ [R] COG0300 Short-chain dehydrogenases of various substr... 97 4e-20 >SMc01175 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 282 Score = 146 bits (368), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 92/243 (37%), Positives = 129/243 (53%), Gaps = 3/243 (1%) Query: 45 WLVGASSGIGAELARQALQAGARVALSARRADVL---QEVAAAHKNAFIAPLDVLCHDAW 101 W+ GASSGIG +A + + G V ++AR + L Q AA + DV Sbjct: 15 WITGASSGIGRAVALRLAEEGYSVVVTARSHEKLVDLQHQAAGPGRMIVLDGDVTDPKDM 74 Query: 102 RDQHARIVEAFGGVDLLVFCAAKYRPERTWEVQEEEAEHTLRTNVASVYSALGAALPDML 161 A+I G V L V A P R +++ E + + N+ V + L + M Sbjct: 75 ERLLAKIEYEHGQVALAVLNAGVALPVRGDDLRREPFDRSFAVNLNGVVNCLVPLVEHMK 134 Query: 162 ARGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILYSDLRPKGLNVYLVNPGFVKT 221 +G G IA+++SVAGY GLP + YG KAALIN+AE L DL G+ + ++ PGFV T Sbjct: 135 TKGHGQIAIMSSVAGYGGLPMSAAYGASKAALINMAESLKFDLDRIGIRLQVICPGFVDT 194 Query: 222 DLTDKNDFPMPAIQTPQQAATAIWRGISEGRFEIHFPRRFTAWLKILRLLPFRLRFMLFQ 281 K FP PA+ + Q+AA I G+ RFEI FP+RFT +K+LR+LP+ L F L Sbjct: 195 QAAPKGGFPRPALVSTQEAADRICAGLKSSRFEITFPKRFTYLVKLLRILPYGLYFALLD 254 Query: 282 RFM 284 R + Sbjct: 255 RLV 257 >mll0352 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 273 Score = 113 bits (283), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 81/233 (34%), Positives = 107/233 (45%), Gaps = 6/233 (2%) Query: 45 WLVGASSGIGAELARQALQAGARVALSARRAD----VLQEVAAAHKNAFIAPLDVLCHDA 100 W+ G SSGIG LAR G VA++A D ++ E A + P DV Sbjct: 14 WVTGGSSGIGRSLARDLASQGYAVAVTALDDDPIDTLIVETAQMPGSVTAFPCDVTDEPR 73 Query: 101 WRDQHARIVEAFGGVDLLVFCAAKYRPERTWEVQEEEAEHTLRTNVASVYSALGAALPDM 160 A I + G + L VF A Y + ++ T N + + L + M Sbjct: 74 MARTVAAIEKELGPIVLAVFNAGNYISTPGEALVVKDFRRTFEVNYFGIINGLVPVVEYM 133 Query: 161 LARGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILYSDLRPKGLNVYLVNPGFVK 220 R G + LV SV Y G P YG KAA+ LAE L D + V ++NPGFV Sbjct: 134 RVRSRGHVVLVGSVTAYFGWPTTAAYGGTKAAINILAESLKYDFDKMNIRVQVINPGFVD 193 Query: 221 TDLTDKNDFPMPAIQTPQQAATAIWRGISEGRFEIHFPRRFTAW-LKILRLLP 272 T LT+KN PMP + +A + + I G FE+ FP R T+W LK L LLP Sbjct: 194 TPLTEKNMLPMPGLMPVTRATRRMIKAIKSGGFEVTFPYR-TSWPLKFLALLP 245 >FN0216 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Fnu Fusobacterium nucleatum Length = 250 Score = 112 bits (279), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 64/241 (26%), Positives = 121/241 (50%), Gaps = 12/241 (4%) Query: 42 LRIWLVGASSGIGAELARQALQAGARVALSARRADVLQEVAAAHKNAFIAPLDVLCHDAW 101 ++I++VG SSGIG LA++ G VA+ + L+++ +KN I +DV + Sbjct: 1 MKIFIVGGSSGIGLSLAKRYASLGNEVAICGTNEEKLKKIEECNKNIKIYKVDVRNKEEL 60 Query: 102 R---DQHARIVEAFGGVDLLVFCAAKYRPERTWEVQEEEAEHTLRTNVASVYSALGAALP 158 + D ++ G +DL++ A Y RT ++ ++EA + N+ V + A Sbjct: 61 KSAIDDFSK-----GNLDLIINSAGIYTNNRTTKLTDKEAYAMIDINLTGVLNTFEAVRD 115 Query: 159 DMLARGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILYSDLRPKGLNVYLVNPGF 218 M G IA+++SVAG + P A+VY K ++ + E S R +N+ + PG+ Sbjct: 116 VMFKNNRGHIAIISSVAGLLDYPKASVYARTKMTIMGVCETYRSFFRNYNINITTIVPGY 175 Query: 219 VKTD----LTDKNDFPMPAIQTPQQAATAIWRGISEGRFEIHFPRRFTAWLKILRLLPFR 274 + TD L++++ P + + +++ I + I E + +I +P + ++R LP + Sbjct: 176 IATDKLKSLSEEDITKKPTVLSEEESTNIIIKAIEEKKEKIIYPLSMKILISVIRKLPKK 235 Query: 275 L 275 L Sbjct: 236 L 236 >VC1119 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Vch Vibrio cholerae Length = 241 Score = 106 bits (265), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 76/243 (31%), Positives = 118/243 (48%), Gaps = 9/243 (3%) Query: 44 IWLVGASSGIGAELARQALQAGARVALSARRADVLQEVAAAHKNAFIAPLDVLCHDAWRD 103 +++ GA+SGIG +LA + G +V R VL + + N F DV H + Sbjct: 4 VFITGATSGIGKQLALDYAKQGWQVIACGRNQSVLDSLHTQYANIFPLAFDVTDHPGTK- 62 Query: 104 QHARIVEAFGGVDLLVFCAAKYRPERTWEVQEEEAEHTLRTNVASVYSALGAALPDMLAR 163 A + + +L + A ++ NV V + + P + Sbjct: 63 --AALAQLPCQPELWILNAGDCEYIDDGKMDVTLMARVFNINVLGVAAVIEGIQPHL--- 117 Query: 164 GSGG--IALVASVAGYVGLPGATVYGPGKAALINLAEILYSDLRPKGLNVYLVNPGFVKT 221 S G +A+V S+A + LP A YG KAA+ LA L D RP G+ V + PGFV T Sbjct: 118 -SCGHRVAIVGSIASELALPRAEAYGASKAAVAYLARTLQLDWRPLGIEVTTIFPGFVAT 176 Query: 222 DLTDKNDFPMPAIQTPQQAATAIWRGISEGRFEIHFPRRFTAWLKILRLLPFRLRFMLFQ 281 LTD+N F MP I T ++AA I G++ G +++FP+RFT +++L LP+ + L + Sbjct: 177 PLTDRNTFAMPMIITVERAAQEIKAGLARGVSQLYFPKRFTWLIRLLGALPYAWQGRLVR 236 Query: 282 RFM 284 R + Sbjct: 237 RLL 239 >BS_yqjQ [R] COG0300 Short-chain dehydrogenases of various substrate specificities Bsu Bacillus subtilis Length = 259 Score = 97.1 bits (240), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 72/240 (30%), Positives = 111/240 (46%), Gaps = 8/240 (3%) Query: 41 GLRIWLVGASSGIGAELARQALQAGARVALSARRADVLQEVAAAHKNAFIAPLDVLCHDA 100 G RIW+ GAS G+G +A GA V LSARR D L E+ + ++ D Sbjct: 6 GKRIWITGASGGLGERIAYLCAAEGAHVLLSARREDRLIEIKRKITEEWSGQCEIFPLDV 65 Query: 101 WR-DQHARIVEAFGGVDLLVFCAAKYRPERTWEVQEEEAEHTLRTNVASVYSALGAALPD 159 R + AR+ + G +D+L+ A E + ++ + NV + + A LP Sbjct: 66 GRLEDIARVRDQIGSIDVLINNAGFGIFETVLDSTLDDMKAMFDVNVFGLIACTKAVLPQ 125 Query: 160 MLARGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILYSDLRPKGLNVYLVNPGFV 219 ML + G I +AS AG + P +++Y K A++ + L +L G+ V VNPG + Sbjct: 126 MLEQKKGHIINIASQAGKIATPKSSLYSATKHAVLGYSNALRMELSGTGIYVTTVNPGPI 185 Query: 220 KTD---LTDK-NDFPMPA---IQTPQQAATAIWRGISEGRFEIHFPRRFTAWLKILRLLP 272 +TD + DK D+ + P A I I + EI+ PR A K+ +L P Sbjct: 186 QTDFFSIADKGGDYAKNVGRWMLDPDDVAAQITAAIFTKKREINLPRLMNAGTKLYQLFP 245