BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0127.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310334|ref|YP_003774426.1| hemolysin activation/secretion ... 593 e-170 gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ... 57 3e-09 >gi|300310334|ref|YP_003774426.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 565 Score = 593 bits (1529), Expect = e-170, Method: Compositional matrix adjust. Identities = 302/540 (55%), Positives = 401/540 (74%), Gaps = 2/540 (0%) Query: 9 LASAQTAP-DAGSILQQLQPALPVAPATSAPSLKLEPRDEPALPQTAPFEVKRFQMTGNT 67 LA+ P DAGS+LQ+LQ A APA + P LK++ D LP +APF VK ++ GNT Sbjct: 26 LAAGPAVPTDAGSLLQELQTAPRTAPAPNQPELKIQSPDASQLPPSAPFLVKEIRIVGNT 85 Query: 68 VFATESLHELVAGEEGKSLTLVQINTLAERITRYYQQRGYPLARAIIPVQTIRDGVVVIQ 127 F +LH LVA +EGK+LTL ++ LA RI+ YYQ G+PLARAIIP QTI +GVVVIQ Sbjct: 86 AFDAATLHALVADQEGKTLTLADLSALAARISSYYQAHGFPLARAIIPAQTISEGVVVIQ 145 Query: 128 VVEARYGQVRLNNKSAVRDSLLQATLDGLQSGAVIEDEALNRSLLLLSDVPGVGVAATLE 187 VVEARYG++ LNN S V LL++ L LQ ++ + +L+RSLLLLSDVPGVGV A L+ Sbjct: 146 VVEARYGKIHLNNSSPVNSRLLESILSSLQKDQMVAERSLDRSLLLLSDVPGVGVEAVLK 205 Query: 188 PGAAVGSSDVTVNTTVNPATLGTVAVDNYGNRYVDRVRVSGNLNVVNPLHHGDLLSATVL 247 PG AVG+SD+ V TT ++G++++DN+GNRY+ R R+SG ++ NPLHHGD+LSA ++ Sbjct: 206 PGEAVGTSDMDVVTTPKAVSIGSMSIDNFGNRYIGRARLSGTASLFNPLHHGDVLSANLV 265 Query: 248 STGSGMNYGRLSYDILVNGQGTHLGAAYSLLRYKLGSELSALDANGTASVASLWGRHPLM 307 STG M+YGR+SYD L+NGQGTH+G AYS++ YKLG + ALDA+G A VASLW +HPL+ Sbjct: 266 STGERMSYGRISYDTLLNGQGTHVGGAYSMVHYKLGDSVRALDAHGNALVASLWLKHPLL 325 Query: 308 RSRQANVYAQVQVDGKRLRDHVDASALRTDRHLLNWVMSINGDIRDSLLAGGISIWSLGW 367 RSR AN+Y Q+Q D K+L D + A+ LRTDRHL NWV+S++G++RDS+L GG+S W +GW Sbjct: 326 RSRDANLYGQIQYDTKKLEDRIGATGLRTDRHLDNWVLSLSGNLRDSVLGGGVSAWGMGW 385 Query: 368 TSGRLFFDDTAAAAADAAAGQTRGGFAKWNANFSRLQGISAGHALYLNLAAQWSDGNLDS 427 T+GR+ +DD AA A+DA QTRGGF+KWN NFSRLQ + +LY N + QWSD NLDS Sbjct: 386 TAGRVGYDDAAAEASDALTAQTRGGFSKWNFNFSRLQFLGRRDSLYANFSVQWSDANLDS 445 Query: 428 AEKMTAGGPYTVRGYDVGAMSGDSGYVGTIEWRHELGNGLSGQWQAIAFLDSAYIKVNQH 487 AEKM+ GGPY+VR YD+GA+SGD+GY+G+IE RHE+G+ G Q +AF+DSA + +N+ Sbjct: 446 AEKMSVGGPYSVRAYDIGAISGDTGYLGSIELRHEMGSMAGGTLQVLAFVDSARVNINRR 505 Query: 488 PWSAGANSGSLSGAGVGLNWNGPDQWRASVSVASRVG-AKPSMVASQSSVRAWATLSKAF 546 W+ G NS +LSG GVG+ W+ W AS VA+++G A S++ S +S RAW +SK F Sbjct: 506 QWTTGQNSVTLSGTGVGMRWSNDALWEASAYVATKIGSANSSLIESSASTRAWLVVSKGF 565 >gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 569 Score = 57.4 bits (137), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 104/464 (22%), Positives = 176/464 (37%), Gaps = 49/464 (10%) Query: 53 TAPFEVKRFQMTGNTVFATESLHELVAGEEGKSLTLVQINTLAERITRYYQQRGYPLARA 112 T FE++RF + GNT+ + + VA G+ I E + Y+ GY + Sbjct: 50 TERFEIRRFVVAGNTLLPQDQVQAAVAPFSGQGRVYGDIQQALEALENLYRSAGYTAVQV 109 Query: 113 IIPVQTIRDGVVVIQVVEARYGQVRLNNKSAVRDSLLQATLDGLQSGAVIEDEALNRSLL 172 +P Q + G V I VVE G+V+++ + ++A++ L+ G ++ S+ Sbjct: 110 HVPEQELSSGEVRIDVVETVVGKVQVSENRHFSQANIRASIPALKEGVPPNLRRISESVQ 169 Query: 173 LLSDVPGVGVAATLEPGAAVGSSDVTVNTTVNPATLGTVAVDNYGNRYVDRVRVSGNLNV 232 L +D P V TL + D + + + +DN G+ R R + Sbjct: 170 LANDNPAKQVNVTLSAAQSGDQIDAKIEVKDDDPLRVMLNLDNTGSADTGRWRTGVAIQH 229 Query: 233 VNPLHHGDLLSATVLST------GSGMNYGRLSYDILVNGQGTHLGAAYSLLRYKLGSEL 286 N L + D ++ +T GS ++ L Y + + G L Y G L Sbjct: 230 SN-LFNRDHVATVAYTTSPDSPSGSKVDLYSLGYRLPLYELGDSLDFIYGKSSVSSGQTL 288 Query: 287 ---SALDANGTASVASLWGRHPLMRSRQANVYAQVQVDGKRLRDHVDASALRTDRHLLNW 343 S L+ G V +L H R+ + + + D K+ VD+S +N Sbjct: 289 ALNSTLNITGRGEVYALRWNHFFARNGEWSSKLVLGADYKK----VDSSC------AING 338 Query: 344 VMSINGDIRDSLLAGGISIWSLGWTSGRL----FFDDTAAAAADAAAGQTRGG------- 392 + +NG ++ L SL +++ R D A + A GQ R Sbjct: 339 GL-LNGATLNTCLPYSTLPLSLTYSAQRQGVGEVIDANIGVARNMATGQARRNDALQVTD 397 Query: 393 ----FAKWNANFSRLQGISAGHALYLNLAAQWS---DGNLD-------SAEKMTAGGPYT 438 F + + G + W GNL + E+ G Sbjct: 398 RYSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQMRLAGNLQLSPHALPAVEQFGLAGATA 457 Query: 439 VRGYDVGAMSGDSGYVGTIE-WRHEL--GNGLSGQWQAIAFLDS 479 VRG+ A+S DSG V E + +L + L G + +AF+D+ Sbjct: 458 VRGFTERAVSADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDA 501 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,727,135 Number of extensions: 73556 Number of successful extensions: 324 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 322 Number of HSP's successfully gapped: 3 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)