BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0037.0119.p
         (323 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310550|ref|YP_003774642.1| TolA-related transport transmem...   348   4e-97

>gi|300310550|ref|YP_003774642.1| TolA-related transport
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 318

 Score =  348 bits (892), Expect = 4e-97,   Method: Compositional matrix adjust.
 Identities = 276/323 (85%), Positives = 295/323 (91%), Gaps = 5/323 (1%)

Query: 1   MSDNAHYTIPKPPGRWRAITLALVMHVALFLFFWIGIRWQSETPLTVEAEIWDPQYKEAA 60
           MSDNA Y IPK PGRWRAITLALVMHVALFLFFWIGIRWQSETPLTVEAEIWDPQYKEAA
Sbjct: 1   MSDNAPYNIPKAPGRWRAITLALVMHVALFLFFWIGIRWQSETPLTVEAEIWDPQYKEAA 60

Query: 61  PLPTPPQPQPEPDPVVEPPKPQPVIEPKTVAPKVIDEPKLDKPDIALQKEKEKKRKEEQE 120
           PLPTPP+PQP+P       KPQP  EPK V PKVIDEPK++KPDIALQKEKE+KRKEEQE
Sbjct: 61  PLPTPPEPQPQPVVEPP--KPQPQPEPKPVPPKVIDEPKVEKPDIALQKEKERKRKEEQE 118

Query: 121 KQEKADKLKAEKEKAEREKKEQAEKDKADREKKEKLEAEKQKKLKEDKQKEEEAKKKADA 180
           KQEK    + EK +  + +KE+AEK+KA+REKKEKLEA+KQKKLKE+KQKE+EAKKKADA
Sbjct: 119 KQEKL---EKEKAEKLKAEKEKAEKEKAEREKKEKLEADKQKKLKEEKQKEDEAKKKADA 175

Query: 181 EKQAADKKKQQQAAADAKAAEARRQEDLKRMMGQATSATGGSGTAEKSQGPKGSPNYANK 240
           EKQAADKKKQQQAAADAKAAEARRQEDLKRMMGQATSATGG+GTAEKSQGPKGSPNYANK
Sbjct: 176 EKQAADKKKQQQAAADAKAAEARRQEDLKRMMGQATSATGGTGTAEKSQGPKGSPNYANK 235

Query: 241 LRAKIRTNTFFDTSGVSGNPAGEYTVELFPDGTVRSVRVNKSSGVPGFDDAVRTAIMKSQ 300
           LRAKIR+NT FD SGVSGNPAGEYTVELFPDGTVRSVRVNK SGVPGFD+AVRTA+MKSQ
Sbjct: 236 LRAKIRSNTVFDNSGVSGNPAGEYTVELFPDGTVRSVRVNKPSGVPGFDEAVRTAVMKSQ 295

Query: 301 PFPADTDGKVPSSFTFTHYPKDQ 323
           PFP DTD KVPSSFTFTHYPKDQ
Sbjct: 296 PFPPDTDNKVPSSFTFTHYPKDQ 318


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.307    0.126    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 632,687
Number of extensions: 21029
Number of successful extensions: 64
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 62
Number of HSP's successfully gapped: 1
Length of query: 323
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 238
Effective length of database: 1,217,395
Effective search space: 289740010
Effective search space used: 289740010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 131 (55.1 bits)