BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0041.p (140 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310602|ref|YP_003774694.1| transporter family protein [Her... 233 3e-63 >gi|300310602|ref|YP_003774694.1| transporter family protein [Herbaspirillum seropedicae SmR1] Length = 140 Score = 233 bits (595), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 118/140 (84%), Positives = 128/140 (91%) Query: 1 MERWIAYAFISMAFAGFTSVIAKLGLTGISSDLGLAIRTCFVFVFVLMFAAAVVPAAQLS 60 MERWI YAF+SMAFAGFTSVIAKLGLTGIS+DLGLAIRTCFVFVFVL+FAAAVVP +QL Sbjct: 1 MERWILYAFVSMAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPTSQLD 60 Query: 61 SVTWQNVLWLGLSGVTTAVSWVFYYKAIKAGDVSTVALIDKGSVIVALLMAVWILNEVIT 120 +TW+N WLGLSGVTTA SWVFYYKAIK GDVSTVALIDKGSV+VA+L+A WILNE IT Sbjct: 61 MLTWKNFFWLGLSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAIT 120 Query: 121 PAKLIGAALIAAGLLVISRG 140 PAKL GAALIA GLLVI+RG Sbjct: 121 PAKLAGAALIAGGLLVIARG 140 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 338,322 Number of extensions: 9510 Number of successful extensions: 53 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 1 Length of query: 140 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 64 Effective length of database: 1,260,010 Effective search space: 80640640 Effective search space used: 80640640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 126 (53.1 bits)