BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0039.p (253 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 68 8e-13 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 65 4e-12 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 64 9e-12 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 62 5e-11 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 60 2e-10 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 67.8 bits (164), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 58/173 (33%), Positives = 84/173 (48%), Gaps = 7/173 (4%) Query: 17 KTVVIVGGKTGIGLGIARAALAAGATVIVASRRITSTQERPELAGLQQVTLDMRDETAVR 76 KT+ I G +G GL IAR L VI A+ R P L+ + LD+ D +++ Sbjct: 2 KTIFITGCSSGFGLEIARYFLQRDWKVI-ATMRTPRHDLLPASPHLRILPLDVADAHSIQ 60 Query: 77 SAFAAIGPFDHLVVTAAPDVGSWGSFMDADMRGVRSYVEGKFLGTWACARYAAPLLRAGG 136 +A A GP D LV A +G+ G+ M VR E LG+ A + P +RA G Sbjct: 61 AAVEAAGPVDVLVNNAG--IGNLGALEAQPMAQVRELFETNTLGSIAMTQAFIPQMRARG 118 Query: 137 T--MTFLSGGMAVRPKPGFTAVTSSFAAVEALAASLAVELAP--TRVNTIRPG 185 + ++ + +R P T+S AA+ A SLA+ELAP +V + PG Sbjct: 119 QGLVINVTSSVTLRALPLLAVYTASKAAINAFTESLALELAPLGVQVRLVLPG 171 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 65.5 bits (158), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 78/245 (31%), Positives = 107/245 (43%), Gaps = 23/245 (9%) Query: 17 KTVVIVGGKTGIGLGIARAALAAGATVIVASRRITSTQERPELAGLQQV-------TLDM 69 K VV+ GG GIGL A+ A GA V V+ RR ++ G V LD Sbjct: 10 KVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPGDAAKLDD 69 Query: 70 RDETAVRSAFAAIGPFDHLVV---TAAPDVGSWGSFMDADMRGVRSYVEGKFLGTWACAR 126 D R A G D L TA+ + G + D G+ + G + Sbjct: 70 LDRLYARVQ-ADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNL---NVRGLLFSVQ 125 Query: 127 YAAPLLRAGGTMTFLSGGMA-VRPKPGFTAVTSSFAAVEALAASLAVELAP--TRVNTIR 183 A PL+ GG + L+G +A V+ PG + +S AAV + A S +L RVN + Sbjct: 126 KALPLMSQGGAIV-LNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVNVVA 184 Query: 184 PGFVETD-MWNFLPADVRAGMMQQVAASFPARRAGQAGDIAHAALFLMT--NPYVTGTVL 240 PG ET M ++L A R GM + P R G+ +I A LFL + + Y+ G L Sbjct: 185 PGGTETRLMRDYLEA--RPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAGVEL 242 Query: 241 DVTGG 245 V GG Sbjct: 243 FVDGG 247 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 64.3 bits (155), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 74/247 (29%), Positives = 111/247 (44%), Gaps = 32/247 (12%) Query: 14 LSGKTVVIVGGKTGIGLGIARAALAAGATVIVASRRITSTQERPELAGLQQVTLDMR--D 71 L GKT ++ GG GIG +AR +A GA VI A + +E AG + ++M Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEE---FAGSEVTLVEMNVAS 64 Query: 72 ETAVRSAFAAIGPFDHLVVTAAPDVGSWGSFMDADMRGVRSYVEGKFLGTWACARYAAPL 131 E +VR+A A + D +V A + G+ ++ ++ + GT A L Sbjct: 65 EESVRAALAGLTQLDIVVNCAG--IIKRGAELETEV--FEQVIAVNLTGTMRVCAAAREL 120 Query: 132 LR--------AGGTMTFLSGGMAVRPKPGFTAVTSSFAAVEALAASLAVELAP--TRVNT 181 LR ++F GG+ PG++A S V L SLA+ A RVN Sbjct: 121 LRQSRGCIVNTASMLSFFGGGLV----PGYSA---SKGGVAQLTKSLAIAYAADGIRVNA 173 Query: 182 IRPGFVETDMWNFLPAD-VRAGMMQQVAASFPARRAGQAGDIAHAALFLMT--NPYVTGT 238 + PG++ T + L D VRAG + A P R GQ D+ FL + ++TG Sbjct: 174 VAPGWIATPLTQALQDDPVRAG---PILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGA 230 Query: 239 VLDVTGG 245 ++ V GG Sbjct: 231 IVPVDGG 237 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 61.6 bits (148), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 45/136 (33%), Positives = 70/136 (51%), Gaps = 13/136 (9%) Query: 120 GTWACARYAAPLLR-----AGGTMTFLSGGMAVRPKPG-FTAVTSSFAAVEALAASLAVE 173 G+ CAR A + GG + LS A PG + +S A++A LA E Sbjct: 118 GSLLCAREAVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKE 177 Query: 174 LAPT--RVNTIRPGFVETDMWNFLPADVRAGMMQQVAASFPARRAGQAGDIAHAALFLMT 231 +A RVN +RPG + TD+ + AG + ++ + P +R G A ++A+A L+L++ Sbjct: 178 VAAEGIRVNAVRPGLIYTDIH---ASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLS 234 Query: 232 NP--YVTGTVLDVTGG 245 Y TGT +DV+GG Sbjct: 235 EEASYATGTFIDVSGG 250 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 78/261 (29%), Positives = 110/261 (42%), Gaps = 47/261 (18%) Query: 14 LSGKTVVIVGGKTGIGLGIARAALAA--GATVIVASRRITSTQERPELAGLQQVTL--DM 69 L K VI GG GLG A A L A GA V+V G Q + L D+ Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADV 62 Query: 70 RDETAVRSAFAAI----GPFDHLVVTAAPDV---------GSWGSFMDADMRGVRSYVEG 116 D+ + +A AAI G D LV A + +D +RG Sbjct: 63 TDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRG------- 115 Query: 117 KFLGTWACARYAAPLLRA--GGTMTFLSG-----GMAVRPKPGFTAVTSSFAAVEALAAS 169 T ++ P +RA G++ +S G + P ++A A V LA + Sbjct: 116 ----TLYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAK---AGVLGLARA 168 Query: 170 LAVELAPT--RVNTIRPGFVETDMWN-FLPADVRAGMMQQVAASFPARRAGQAGDIAHAA 226 +A E P RVN+I PG + TD+ L + +A ++A + P R G+A DI A Sbjct: 169 MAREFGPEGIRVNSITPGLIGTDIIKGKLTEEKKA----EIAETIPLARLGRADDIGGAC 224 Query: 227 LFLMTN--PYVTGTVLDVTGG 245 +FL ++ Y TG LDV GG Sbjct: 225 VFLGSDLSQYCTGITLDVNGG 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 644,345 Number of extensions: 24880 Number of successful extensions: 122 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 13 Length of query: 253 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 170 Effective length of database: 1,226,865 Effective search space: 208567050 Effective search space used: 208567050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)