BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0016.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydr... 430 e-122 gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydro... 151 4e-38 gi|300311050|ref|YP_003775142.1| esterase_lipase superfamily pro... 69 3e-13 gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 68 6e-13 gi|300313799|ref|YP_003777891.1| arylesterase [Herbaspirillum se... 66 3e-12 >gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydrolase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 430 bits (1106), Expect = e-122, Method: Compositional matrix adjust. Identities = 222/258 (86%), Positives = 238/258 (92%) Query: 1 MPFATLNGIKLHYQLDGDAALPVLVLSNSLGTSLAMWDPQIPELSKHFRVLRYDTRGHGQ 60 MPFA +N I+LHYQL+GDA+LPVLVLSNSLGTSL+MWDPQ+P+ +HFRVLRYDTRGHGQ Sbjct: 1 MPFANVNAIRLHYQLEGDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQ 60 Query: 61 SGVTPGPYTIAQLGSDVIALLDQLDIKQAHFCGLSMGGSTFMWLAVHHPDRVAKLILCNT 120 S VTPG +IAQLGSDVIALLD L I QAHFCGLSMGGSTFMWLAVHHP R+ KLILCNT Sbjct: 61 SEVTPGECSIAQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRINKLILCNT 120 Query: 121 GAKIGTPEAWNSRIETVQRDGLSAIAAAVVSRWLTPEYAEQHPEQVQALTAMLLATPAEG 180 GAKIGT EAWNSRIETV+R+GL AIA AVVSRWLTPEYA+ HP+QVQALTAMLLATPAEG Sbjct: 121 GAKIGTAEAWNSRIETVRREGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEG 180 Query: 181 YAGACAAVRDNDLREAIAGIRAPTLVIAGSGDVPTPPADAQFMRATIPGALYVEFDAAHI 240 YA ACAAVRD+DLREAIAGIRAPTL+IAGSGDVPTPPADAQFMRATIPGA YVEFDAAHI Sbjct: 181 YAAACAAVRDHDLREAIAGIRAPTLIIAGSGDVPTPPADAQFMRATIPGAQYVEFDAAHI 240 Query: 241 SNQQQPRAFTQAVVQFLT 258 SNQQQ AFTQAVVQFLT Sbjct: 241 SNQQQAAAFTQAVVQFLT 258 >gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 151 bits (382), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 101/254 (39%), Positives = 130/254 (51%), Gaps = 7/254 (2%) Query: 11 LHYQL----DGDAALPVLVLSNSLGTSLAMWDPQIPELSKHFRVLRYDTRGHGQSGVTPG 66 LHY + +G A +VLS++LGT L MWD +L+ RV+ YD RGHG S G Sbjct: 60 LHYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADG 119 Query: 67 PYTIAQLGSDVIALLDQLDIKQAHFCGLSMGGSTFMWLAVHHPDRVAKLILCNTGAKI-- 124 Y++A L D LL +LD + GLSMGG LA+ HP V L+L NT + Sbjct: 120 LYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPD 179 Query: 125 GTPEAWNSRIETVQRDGLSAIAAAVVSRWLTPEYAEQHPEQVQALTAMLLATPAEGYAGA 184 EAW RI TV+ +G+ IA AV+ R+ + V L+ T A GY G Sbjct: 180 AAREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGC 239 Query: 185 CAAVRDNDLREAIAGIRAPTLVIAGSGDVPTPPADAQFMRATIPGA-LYVEFDAAHISNQ 243 C AV D + IR PTLVIAG D TP A AQ + I GA L V A+H+S Sbjct: 240 CHAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAV 299 Query: 244 QQPRAFTQAVVQFL 257 +QP F + V F+ Sbjct: 300 EQPALFAELVCGFI 313 >gi|300311050|ref|YP_003775142.1| esterase_lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 69.3 bits (168), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 43/121 (35%), Positives = 64/121 (52%), Gaps = 6/121 (4%) Query: 1 MPFATLNGIKLHYQLDGDAALPVLVLSNSLGTSLAMWD--PQIPELSKHFRVLRYDTRGH 58 MPFA + K+HYQ++G+ P L+L + +SL W L +H+RV+ +D GH Sbjct: 1 MPFAISDQRKIHYQVEGEG--PPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGH 58 Query: 59 GQSGV--TPGPYTIAQLGSDVIALLDQLDIKQAHFCGLSMGGSTFMWLAVHHPDRVAKLI 116 GQS Y+ Q D +A+LD +++ HF G S+GG L P+R+ LI Sbjct: 59 GQSDKPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLI 118 Query: 117 L 117 L Sbjct: 119 L 119 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 68.2 bits (165), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 84/275 (30%), Positives = 111/275 (40%), Gaps = 24/275 (8%) Query: 5 TLNGIKLHYQLDGD-AALPVLVLSNSLGTSLAMWDPQIPELSKHFRVLRYDTRGHGQSGV 63 TL+ + H GD P+L+L G D L +R + D RG GQS Sbjct: 9 TLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGD-LAQRLPPGYRCIALDQRGRGQSDW 67 Query: 64 TPGP--YTIAQLGSDVIALLDQLDIKQAHFCGLSMGGSTFMWLAVHHPDRVAKLILCNT- 120 G YT A + +DV AL+ +L+++Q G SMGG + A HP +V +LI+ + Sbjct: 68 DAGANYYTDAYV-ADVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAG 126 Query: 121 -GAKIGTPEAWNSRIE--TVQRDGLSAIAAAVVSRWLTPEYAEQHPEQVQALTAMLLATP 177 GA +P A R E T S A+ R L P E+ + Q L ML Sbjct: 127 PGAFENSPGATRIRRELATTPSHFASWDEASTFMRALRPSVTEE--ARQQRLHNMLKPAA 184 Query: 178 AEGYAGACAAV------------RDNDLREAIAGIRAPTLVIAGSGDVPTPPADAQFMRA 225 GY V R DLR I + PTLV+ G A MR Sbjct: 185 QGGYTWRYDHVGISSTRLSPDLSRVVDLRPHIMALMCPTLVVRGERSDYLSAEMAAEMRQ 244 Query: 226 TIPGALYVEF-DAAHISNQQQPRAFTQAVVQFLTE 259 L DA H + QP AF + V QFL + Sbjct: 245 LNARILTSTIADAGHYIHDDQPEAFAEVVRQFLAQ 279 >gi|300313799|ref|YP_003777891.1| arylesterase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 62/242 (25%), Positives = 100/242 (41%), Gaps = 18/242 (7%) Query: 5 TLNGIKLHYQLDGDAALPVLVLSNSLGTSLAMWDPQIPELSKH-FRVLRYDTRGHGQSGV 63 T +G +++Y+ D + PV V + MW+ Q+ L++ +RV+ YD RG G+S Sbjct: 6 TRDGTEIYYK-DWGSGQPV-VFGHGWPLDGDMWEYQMNFLAERGYRVIAYDRRGFGRSSQ 63 Query: 64 TPGPYTIAQLGSDVIALLDQLDIKQAHFCGLSMGGSTF-MWLAVHHPDRVAKLILCNT-- 120 Y D+ L++ LD++ A G SMGG ++ H RVAK L Sbjct: 64 PWSGYDYDTFADDLAELMEHLDLQGATLVGFSMGGGDVARYIGRHGSKRVAKAALLGAVT 123 Query: 121 ---GAKIGTPEAWNSRIETVQRDGLSAIAAAVVSRWLTPEYAEQHPEQVQALTAMLLA-- 175 G P+ + + ++GL A +S + + P + A++ +LA Sbjct: 124 PIFGQAPDFPQGVPAEVFAGIKEGLLKDRAQFISDFAAVFFGTNRPGKADAVSPGVLAQT 183 Query: 176 ------TPAEGYAGACAAVRDNDLREAIAGIRAPTLVIAGSGDVPTP-PADAQFMRATIP 228 +G A D R I PTLVI G D P + + + +P Sbjct: 184 FNIAMLASLKGTLDCVTAFSGTDFRADIKKFNFPTLVIHGDDDQIVPLQSTGKLVAEMVP 243 Query: 229 GA 230 GA Sbjct: 244 GA 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 926,949 Number of extensions: 41134 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 6 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)