BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0013.p (397 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 747 0.0 gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 500 e-143 gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 66 5e-12 gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas... 56 4e-09 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 747 bits (1929), Expect = 0.0, Method: Compositional matrix adjust. Identities = 375/398 (94%), Positives = 385/398 (96%), Gaps = 1/398 (0%) Query: 1 MDTPQH-LHAAAPALIDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGTVGVGE 59 M+T H + AA PALIDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDG++GVGE Sbjct: 1 METTLHNITAAGPALIDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSIGVGE 60 Query: 60 GTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADASLIPAIMARLNKAIQDNRFAKCAIET 119 GTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADAS IPAIMARLNKAI DNRFAKCAIET Sbjct: 61 GTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADASQIPAIMARLNKAICDNRFAKCAIET 120 Query: 120 ALFDALGQRTGLPVSQLLGGRARESLPIAWTLASGDTAKDIDEAERMLAARRHKIFKLKI 179 ALFDALGQRTGLPVSQLLGGR+RESLPIAWTLASGDTAKDIDEAERMLA RRH IFKLKI Sbjct: 121 ALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRHNIFKLKI 180 Query: 180 GRREVEADVAHVAAIKKALGDRGSVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASA 239 GRREV+ADVAHVAAIKKALGDRG+VRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVAS Sbjct: 181 GRREVDADVAHVAAIKKALGDRGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASP 240 Query: 240 PALARLKGKHAIAIMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDA 299 ALARLKG+HAIAIMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDA Sbjct: 241 QALARLKGRHAIAIMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDA 300 Query: 300 AGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAI 359 AGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAI Sbjct: 301 AGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAI 360 Query: 360 PNRPGLGIVLDEERVQYFRRDRKQGATVTAVRPATLAG 397 P+RPGLGI LDEER+QYFRRDRKQGATVTAVRPA LAG Sbjct: 361 PHRPGLGITLDEERLQYFRRDRKQGATVTAVRPAKLAG 398 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 500 bits (1288), Expect = e-143, Method: Compositional matrix adjust. Identities = 251/370 (67%), Positives = 294/370 (79%), Gaps = 2/370 (0%) Query: 15 IDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGTVGVGEGTTIGGLAYGAESPE 74 I+ VE L+D+PTIRPH+LSM TM+ QTL+LVR+ +DG G GE TTIGGLAYG ESPE Sbjct: 10 IETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGGLAYGEESPE 69 Query: 75 GMKLAIDTYFAPLLLGADASLIPAIMARLNKAIQDNRFAKCAIETALFDALGQRTGLPVS 134 +K+ IDTY PLLLGADA+ + A M +++ +Q NRFAKCAIETAL DAL +R G+P+S Sbjct: 70 SIKVNIDTYITPLLLGADANQVAARMHHIDEHVQGNRFAKCAIETALSDALARRLGVPLS 129 Query: 135 QLLGGRARESLPIAWTLASGDTAKDIDEAERMLAARRHKIFKLKIGRREVEADVAHVAAI 194 L GGR R+SLPIAWTLASGDTA+DIDEAE+MLA RRH +FKLKIG R V+ D AHVAAI Sbjct: 130 TLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQQDCAHVAAI 189 Query: 195 KKALGDRGSVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASAP--ALARLKGKHAIA 252 K+ALG+RGSVRVDVN AW+E EA G+ L DAG +LVEQP+A++ L RL+ K+ IA Sbjct: 190 KRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAASNHDGLRRLRQKNRIA 249 Query: 253 IMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEG 312 IMADESL GP A+ LAR G ADVFA+KI QSGGL AQQVAAI AA +ELYGGTMLEG Sbjct: 250 IMADESLHGPQDAYQLARAGAADVFAVKINQSGGLAGAQQVAAIASAAQVELYGGTMLEG 309 Query: 313 AIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAIPNRPGLGIVLDEE 372 AIGT+ASA FATF L WG+ELFGPLLLTEEILA PL+Y+DF L +P PGLGI LDE+ Sbjct: 310 AIGTMASAQLFATFGTLAWGSELFGPLLLTEEILATPLRYRDFALELPTGPGLGITLDED 369 Query: 373 RVQYFRRDRK 382 RV RRDRK Sbjct: 370 RVNALRRDRK 379 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 66.2 bits (160), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 74/310 (23%), Positives = 129/310 (41%), Gaps = 27/310 (8%) Query: 90 GADASLIPAIMARLNKAIQDNRFAKCAIETALFDALGQRTGLPVSQLLGGRARESLPIAW 149 A A L P A +KA+ + A A E A DA + +P+ LLGG R+ +P + Sbjct: 92 NAGAELAPGSHA--SKAVSN---AYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSA 146 Query: 150 TL---------------ASGDTAKD---IDEAERMLAARRHKIFKLKIGRREVEADVAHV 191 L A G+ + + +A +M+ + KLK G E +VA + Sbjct: 147 YLFFKYAQHIDSPYAPDAWGEALSEEQIVAQARKMIEENGFQSIKLKAGALAPEHEVACI 206 Query: 192 AAIKKALGDRGSVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASAPALARLKGKHAI 251 A++KA D +R+D N WS A R +A L+ + E P +A L + + Sbjct: 207 KALRKAFPD-APLRIDPNGNWSLQTAIR-MAELLGDDLQYYEDPTPGLEGMAELHRRTGL 264 Query: 252 AIMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLE 311 + + +T + + GGLR QQ+A + D G+ + + Sbjct: 265 PLATNMVVTDFDELRRSVALDSVQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNSH 324 Query: 312 GAIGTIASAHAFATFRELQWGTELFGPLLLTEE--ILAEPLQYQDFQLAIPNRPGLGIVL 369 I +A +H A L + + P +E I L ++ + I + PGLG+ + Sbjct: 325 LGISLMAMSHVAAAVENLSYACDTHYPWQEPDEEVIQGGKLPIRNGCVQITDAPGLGVEV 384 Query: 370 DEERVQYFRR 379 D+++++ + Sbjct: 385 DQDQLRKLHQ 394 >gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 77/286 (26%), Positives = 123/286 (43%), Gaps = 32/286 (11%) Query: 113 AKCAIETALFDALGQRTGLPVSQLLGGRARESLPIAWTLASGDTAKDIDEAERMLAARRH 172 A I+ AL+D G+ G+PV QLLGG R S+ + ++ GD D A + AR Sbjct: 82 ALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRV-YSWIGGDRPADTAAAAKSAVARGF 140 Query: 173 KIFKLKIGRRE---------VEADVAHVAAIKKALGDRGSVRVDVNMAWSELEAQRGLAG 223 K+ G E +E +A+V A+++A+G + VD + + A+ + Sbjct: 141 TAVKMN-GTEELQFIDSHDKIELTLANVQAVREAVGPNVGIGVDFHGRVHKPMAKALIKE 199 Query: 224 LVDAGCELVEQPVAS--APALARLKGKHAIAIMADESLTGPASAFALARI---GGADVFA 278 L +E+PV S A AL + + I E L S + RI G D+ Sbjct: 200 LEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLY---SRWDFKRILSEGYVDIIQ 256 Query: 279 IKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEGAIGTIASAHAF--------ATFRELQ 330 + +GG+ +++A + +A + L L +G IA A A +E Sbjct: 257 PDVSHAGGITETRKIAMMAEAYDVAL----ALHCPLGPIALASCLQVDAGSYNAFIQEQS 312 Query: 331 WGTELFGPLLLTEEILAEP-LQYQDFQLAIPNRPGLGIVLDEERVQ 375 G L + I + Y+D + IP PGLGI ++EE V+ Sbjct: 313 LGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVR 358 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,198,458 Number of extensions: 48627 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 4 Length of query: 397 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 310 Effective length of database: 1,207,925 Effective search space: 374456750 Effective search space used: 374456750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)