BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0012.p (311 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba... 613 e-177 gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba... 317 6e-88 gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy... 126 2e-30 gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase... 63 4e-11 >gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 311 Score = 613 bits (1582), Expect = e-177, Method: Compositional matrix adjust. Identities = 297/311 (95%), Positives = 304/311 (97%) Query: 1 MAAKIFSTPEVQDFLRLASGLDTEGGSPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVA 60 MAAKIFST EVQDFLRLASGLDTEGG+PRTKQIVHRILSDLFKTIEDLEITSDEYWAGVA Sbjct: 1 MAAKIFSTREVQDFLRLASGLDTEGGNPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVA 60 Query: 61 YVNRLGAASEAGLLSPGLGLDRFLDMRMDAEDAALGISGGTPRTIEGPLYVAGAPVEQGF 120 Y+NRLG A EAGLLSPGLGLDRFLDMRMDAEDAALGI+GGTPRTIEGPLYVAGAPVEQGF Sbjct: 61 YINRLGTAHEAGLLSPGLGLDRFLDMRMDAEDAALGIAGGTPRTIEGPLYVAGAPVEQGF 120 Query: 121 ARLDDGSDKNGQTIIMHGTVYGSDGKPMAGAQVEVWHANTKGFYSHFDPTGEQKPFNMRR 180 ARLDDGSDK+GQTIIMHGTVYG+DGKPM GAQVEVWHANTKGFYSHFDPTGEQKPFNMRR Sbjct: 121 ARLDDGSDKSGQTIIMHGTVYGADGKPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRR 180 Query: 181 TIITDAQGRYKFRSIMPAAYGCPPAGPTQGLLNQLGRHGNRPAHIHFFVTADGHRKLTTQ 240 TIITDAQGRYKFRSIMP AYGCPPAGPTQGLLN LGRHGNRPAHIHFFVTA+GHRKLTTQ Sbjct: 181 TIITDAQGRYKFRSIMPTAYGCPPAGPTQGLLNALGRHGNRPAHIHFFVTAEGHRKLTTQ 240 Query: 241 INIEGDPLIYDDFAYATREGLVPPLVERTDAASIKAEGLEGPFAEIVFDINLTSLVNGAD 300 INIEGDPLIYDDFAYATREGLVPPLVERTDAASIKAEGLEGPFAEIVFDI LTSLVNGAD Sbjct: 241 INIEGDPLIYDDFAYATREGLVPPLVERTDAASIKAEGLEGPFAEIVFDIKLTSLVNGAD 300 Query: 301 NQVVDRPRLAA 311 NQ+VDRPRLAA Sbjct: 301 NQIVDRPRLAA 311 >gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 317 bits (813), Expect = 6e-88, Method: Compositional matrix adjust. Identities = 155/286 (54%), Positives = 199/286 (69%), Gaps = 3/286 (1%) Query: 26 GSPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYVNRLGAASEAGLLSPGLGLDRFLD 85 G+ R K +V+R++ D+F TIE+L++ +E+WA V Y+ G + E GL++ GLG + FLD Sbjct: 21 GNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAGKSGEFGLIAAGLGFEHFLD 80 Query: 86 MRMDAEDAALGISGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKNGQTIIMHGTVYGSDG 145 +RMD +A G+ GGTPRTIEGPLYV+GAP ++GFAR+D + G T+ M G V+ G Sbjct: 81 LRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDKDP-QPGDTLFMQGQVFDEHG 139 Query: 146 KPMAGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPAAYGCPPA 205 KP+A A VEVWHAN G YS FDP +Q PFN+R +I TD QGRY+FRS +P Y PP Sbjct: 140 KPLANALVEVWHANHLGRYSFFDP--DQSPFNLRCSIRTDEQGRYRFRSRVPVGYSVPPG 197 Query: 206 GPTQGLLNQLGRHGNRPAHIHFFVTADGHRKLTTQINIEGDPLIYDDFAYATREGLVPPL 265 G T LL +LGRHG+RPAHIHFFVT G+RKLTTQINI GDP ++DDFA+ATREGLVP L Sbjct: 198 GATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPYLWDDFAFATREGLVPEL 257 Query: 266 VERTDAASIKAEGLEGPFAEIVFDINLTSLVNGADNQVVDRPRLAA 311 TD A I+ ++ PF I F+ NL ++RPRLAA Sbjct: 258 KHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQADLPKADINRPRLAA 303 >gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 82/272 (30%), Positives = 132/272 (48%), Gaps = 14/272 (5%) Query: 29 RTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYVNRLGAAS-----EAGLLSPGLGLDRF 83 R ++I+ ++ L ++++T E++AG+ ++ G E LLS LGL Sbjct: 32 RLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSDQRQEFILLSDTLGLSTL 91 Query: 84 LDMRMDAEDAALGISGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKNGQTIIMHGTVYGS 143 + + + + A G T T+ GP +V AP A + +G+ G+ + G+V G Sbjct: 92 VMAQQNHKPA-----GCTEATVFGPFHVEDAPQYPLGADIANGA--KGEPCFVSGSVRGR 144 Query: 144 DGKPMAGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPAAYGCP 203 +G+P+A A++EVW A+ GFY + + R T+ TDA+GRY FRSI+ Y P Sbjct: 145 NGRPLAHARMEVWQADENGFYD--VQYADLDQYRARGTLFTDAEGRYHFRSILANPYPIP 202 Query: 204 PAGPTQGLLNQLGRHGNRPAHIHFFVTADGHRKLTTQINIEGDPLIYDDFAYATREGLVP 263 GP +L +LGRH RPAH+HF + A+G+ L T + D + D + R L+ Sbjct: 203 HDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLDSDAVFGVRSTLIA 262 Query: 264 PLVERTDAASIKAEGLEGPFAEIVFDINLTSL 295 + E PF + +D L L Sbjct: 263 DWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPL 294 >gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase beta chain protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 62.8 bits (151), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 46/136 (33%), Positives = 61/136 (44%), Gaps = 33/136 (24%) Query: 131 GQTIIMHGTVYGSDGKPMAGAQVEVWHANTKGFYSH--------FDPTGEQKPFNMRRTI 182 G+ I++ G V DGKP+ + +EVW N G Y H DP F + Sbjct: 68 GEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPN-----FTGFGKM 122 Query: 183 ITDAQGRYKFRSIMPAAYGCPPAGPTQGLLNQLGRHGN--RPAHIHFFVTADGH-RKLTT 239 +TD GRY+F SI P Y G H RPAHIHF + + + ++L T Sbjct: 123 LTDDNGRYRFVSIKPGPY-------------PWGNHHKAWRPAHIHFSLFGNVYAQRLVT 169 Query: 240 QINIEGDPLIYDDFAY 255 Q+ DPL FAY Sbjct: 170 QMYFPNDPL----FAY 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.138 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,094,255 Number of extensions: 50766 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 122 Number of HSP's successfully gapped: 4 Length of query: 311 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 226 Effective length of database: 1,217,395 Effective search space: 275131270 Effective search space used: 275131270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)