BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0007.p (262 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310643|ref|YP_003774735.1| cis-diol dehydrogenase [Herbasp... 426 e-121 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 101 7e-23 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 94 1e-20 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 85 6e-18 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 85 7e-18 >gi|300310643|ref|YP_003774735.1| cis-diol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 426 bits (1096), Expect = e-121, Method: Compositional matrix adjust. Identities = 208/256 (81%), Positives = 231/256 (90%) Query: 7 RARFAGKVAVVTGAAQGILRGVALGIAAEGGQVLMVDRAGFIHEVAMEAPEGRATGFVAD 66 ++RFAGKV VVTGAAQGILRGVALGIA EGGQVL+VDRA F+H+VA EA GR G VAD Sbjct: 2 KSRFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAEAAGGRGKGLVAD 61 Query: 67 LETHDGAQAAMARAIELHGRIDLLVNGVGGAIRMRPFAEFEADQIDAEIRRSLMPTLYGC 126 LE++ GAQAAM A++ +GRID+L+NGVGGAIRMRP+A++ QIDAEIRRSLMPTLYGC Sbjct: 62 LESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYGC 121 Query: 127 HAVLPHMLRAGRGTIVNVSSNATRGIRRVPYSAAKGGVNAMTMALAMEYGEQGIRVVATA 186 HAVLP +L+ GRG IVNVSSNATRGIRRVPYSAAKGGVNA+T ALAMEYGE GIRVVATA Sbjct: 122 HAVLPQLLQQGRGVIVNVSSNATRGIRRVPYSAAKGGVNALTAALAMEYGELGIRVVATA 181 Query: 187 PGGTKAPPRRVPRNVEGDSAQEQAWMQEAVQQVTGSTFFKRYATLEEQIAPILFMASDEA 246 PGGT+APPRRVPRN EGDS QEQ WMQEAVQQVTGS +FKRY+TLEEQIAPILF+ASD+A Sbjct: 182 PGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDDA 241 Query: 247 SYITGAVLPVAGGDNG 262 SYITGAVLPVAGGDNG Sbjct: 242 SYITGAVLPVAGGDNG 257 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 101 bits (251), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 87/269 (32%), Positives = 127/269 (47%), Gaps = 28/269 (10%) Query: 1 MSAALPR--ARFAGKVAVVTGAAQGILRGVALGIAAEGGQVLMVDR-AGFIHEVAMEAPE 57 MSA P RF GKV +VTGAA GI A + EG +VL+ DR A + +V P Sbjct: 1 MSALNPSRLQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPP 60 Query: 58 GRATGFVADLETHDGAQAAMARAIELHGRIDLLVNGVGGAIRMRPFAEFEADQIDAEIRR 117 R D+ H+ + + AIE G++D+LV+ G FAE ++ E Sbjct: 61 ERTAARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAG------VFAEGNVTEVSPEDWH 114 Query: 118 SLMPT-----LYGCHAVLPHMLRAGRGTIVNVSSNA--TRGIRRVPYSAAKGGVNAMTMA 170 + T YG LPH L RG IVNV+S + Y+A+KG V +T A Sbjct: 115 RVQATNVNGVFYGAREALPH-LEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRA 173 Query: 171 LAMEYGEQGIRVVATAPGGTKAPPRRVPRNVEGDSAQEQAWMQEAVQQVTGSTFFKRYAT 230 +A+++G +G+R+ A P T + D A + + + +++ R A Sbjct: 174 MALDFGRKGVRINAVCPSLTHTA-------MTADMADDPPLLDKFAERIA----LGRGAD 222 Query: 231 LEEQIAPILFMASDEASYITGAVLPVAGG 259 E A I F+AS +AS++ G LPV GG Sbjct: 223 PLEIAAVITFLASPDASFVNGVNLPVDGG 251 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 94.0 bits (232), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 87/259 (33%), Positives = 123/259 (47%), Gaps = 27/259 (10%) Query: 12 GKVAVVTGAAQGILRGVALGIAAEGGQ-VLMVDRAGFIHEVA--MEAPEGRAT---GFVA 65 GKVA++TGA+ GI R AL A G +L R + +VA + A GRA+ G V Sbjct: 6 GKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAGDVG 65 Query: 66 DLETHDGAQAAMARAIELHGRIDLLVNGVGGAIRMRPFAEFEADQIDAEIRRSLMPTLYG 125 + TH + +A A++ G +D+ +N G +P AE Q + +L G Sbjct: 66 EAATH---ERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLG 122 Query: 126 CHAVLPHMLRAGRGTIVNVSS--NATRGIRRV-PYSAAKGGVNAMTMALAMEYGEQGIRV 182 +P ML G G IV SS ++ G+ + Y AAK G+ + +A +Y QGIR Sbjct: 123 ARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRA 182 Query: 183 VATAPGGTKAPPRRVPRNVEGDSAQEQAWMQEAVQQVTGSTFFKRYATLEEQIAPILFMA 242 A PGG V ++ GD Q+Q W G KR A EE A LF+A Sbjct: 183 NALLPGG-------VDTDMGGDPQQKQ-W-------AAGLHAMKRIAQPEEIAAAALFLA 227 Query: 243 SDEASYITGAVLPVAGGDN 261 S AS++TG L GG++ Sbjct: 228 SPMASFVTGTALYADGGNS 246 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 85.1 bits (209), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 76/262 (29%), Positives = 120/262 (45%), Gaps = 25/262 (9%) Query: 9 RFAGKVAVVTGAAQGILRGVALGIAAEGGQVLMVD--RAGFIHEVA-MEAPEGRATGFVA 65 R KVA+VTG G G+A A EG +++ D AG + V ++A GRA A Sbjct: 2 RLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAKA 61 Query: 66 DLETHDGAQAAMARAIELHGRIDLLVNGVGGAIRMRPFAEFEADQIDAEIRRSLMPTLYG 125 D+ +A A+E G++D++VN G R RP E E D+ D ++ Sbjct: 62 DVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFLS 121 Query: 126 CHAVLPHMLRAGRGTIVNVSSNATRGIRRVP----YSAAKGGVNAMTMALAMEYGEQGIR 181 +P+ + G G +N++S A GIR P Y+ +KG V + ++A E G IR Sbjct: 122 AKTFVPYFRQVGGGAFINIASTA--GIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIR 179 Query: 182 VVATAP----GGTKAPPRRVPRNVEGDSAQEQAWMQEAVQQVTGSTFFKRYATLEEQIAP 237 V P G + VP D+ + + ++ + R++T E+ Sbjct: 180 VNCVNPVISATGLLSEFMGVP-----DTPENR-------KKFVATIPMGRFSTPEDIANA 227 Query: 238 ILFMASDEASYITGAVLPVAGG 259 L++ SDEA ++TG + V GG Sbjct: 228 CLYLGSDEAEFVTGVCIEVDGG 249 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 84.7 bits (208), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 83/252 (32%), Positives = 120/252 (47%), Gaps = 28/252 (11%) Query: 12 GKVAVVTGAAQGILRGVALGIAAEGGQVLMVDRAGFIHEVAMEAPEGRATGFVADLETHD 71 GK A+VTG QGI VA + A G +V+ F HE E T ++ + + Sbjct: 10 GKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHE---EFAGSEVTLVEMNVASEE 66 Query: 72 GAQAAMARAIELHGRIDLLVNGVGGAIRMRPFAEFEADQIDAEIRRSLMPTLYGCHAVLP 131 +AA+A +L D++VN G ++ AE E + + I +L T+ C A Sbjct: 67 SVRAALAGLTQL----DIVVNCAG---IIKRGAELETEVFEQVIAVNLTGTMRVCAAAR- 118 Query: 132 HMLRAGRGTIVNVSSNATR-GIRRVP-YSAAKGGVNAMTMALAMEYGEQGIRVVATAPGG 189 +LR RG IVN +S + G VP YSA+KGGV +T +LA+ Y GIRV A APG Sbjct: 119 ELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGW 178 Query: 190 TKAPPRRVPRNVEGDSAQEQAWMQEAVQQ--VTGSTFFKRYATLEEQIAPILFMASDEAS 247 P QA + V+ + T R+ E+ + F+ S AS Sbjct: 179 IATP-------------LTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAAS 225 Query: 248 YITGAVLPVAGG 259 ++TGA++PV GG Sbjct: 226 FMTGAIVPVDGG 237 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 873,484 Number of extensions: 37046 Number of successful extensions: 272 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 40 Length of query: 262 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 179 Effective length of database: 1,226,865 Effective search space: 219608835 Effective search space used: 219608835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)