BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0005.p (455 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 766 0.0 gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 236 3e-63 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 171 8e-44 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 120 2e-28 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 119 5e-28 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 766 bits (1977), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/455 (90%), Positives = 428/455 (94%) Query: 1 MKTQEVSQIIDGARFTQFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA 60 MKT +VSQIIDGARFT+FHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA Sbjct: 1 MKTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA 60 Query: 61 LGSYALFGMMFGAFVFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPGEFGIFRFLAG 120 LGSYALFGMMFGAF+FGPLADRIGRKKGVAISFVLFSVSTFLSGFATSP EFG++RFLAG Sbjct: 61 LGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAG 120 Query: 121 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA 180 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA Sbjct: 121 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA 180 Query: 181 AAVPLVLLPVILWKLPESVGFLLRAGKQEQARAMLAQVAPQAALGADVQLVQSDAKGSGA 240 AAVPLVLLPVILWKLPESVGFLLR G+ EQARAML QV PQA + D QLVQ+D KG+GA Sbjct: 181 AAVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQARIATDTQLVQADVKGAGA 240 Query: 241 PMLDLFKQERTLGTLMIWVAFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGG 300 PML LFKQ+R +GTLMIWV+FFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGG Sbjct: 241 PMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGG 300 Query: 301 VVGAIGGGWLGDRFTLPKVVVSFFAVGTVAIALLGFNSPTPVLYLLIIVAGATTIGTQIL 360 VVGAIGGGWLGDRFTLPKVVVSFF VGTVAIALLGFNSPTPVLYLLIIVAGATTIGTQIL Sbjct: 301 VVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLIIVAGATTIGTQIL 360 Query: 361 LYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAVPGLVA 420 LYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFA+PGLVA Sbjct: 361 LYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPGLVA 420 Query: 421 ALAIGIFLLRYRHGQRAVAAETAPTVTATPISRSV 455 ALA+G+F+LRYRHG +A A A SRSV Sbjct: 421 ALAMGLFMLRYRHGSQASHAAPAAKAPPAAASRSV 455 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 236 bits (602), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 147/428 (34%), Positives = 243/428 (56%), Gaps = 5/428 (1%) Query: 1 MKTQEVSQIIDGARFTQFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA 60 + T V Q+++ RF F + ++ LC L L+ DG+D+ G V P ++ +W +S G Sbjct: 9 LPTVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGP 68 Query: 61 LGSYALFGMMFGAFVFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPGEFGIFRFLAG 120 + LFGM+ G+ V P+ DR GR+ + +S + F++ + T+ + + RF+ G Sbjct: 69 VFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITG 128 Query: 121 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA 180 G G +MPNA+AL+ E++P R T + ++ G+++G + ++P +GW ++F+ Sbjct: 129 FGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWV 188 Query: 181 -AAVPLVL-LPVILWKLPESVGFL-LRAGKQEQARAMLAQVAPQAALGADVQLVQSDAKG 237 AVPLVL L +++W LPES+ FL L + Q L ++ P + ++V + K Sbjct: 189 GGAVPLVLGLAMLVW-LPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKA 247 Query: 238 SGAPMLDLFKQERTLGTLMIWVAFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALN 297 G P+ LF+ R T+++W+ F L+ +Y L +WLP L+ AGYS ++ +L Sbjct: 248 EGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSLQ 307 Query: 298 FGGVVGAIGGGWLGDRFTLPKVVVSFFAVGTVAIALLGFNSPTP-VLYLLIIVAGATTIG 356 GGV+G + G RF +V+ S F + ++IAL+G + P +L++ +IVAG +G Sbjct: 308 VGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIVG 367 Query: 357 TQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAVP 416 Q + A FYP +RSTG+GWA G+GR G++VGP++GG L+A FL AVP Sbjct: 368 GQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAAAVP 427 Query: 417 GLVAALAI 424 L++AL I Sbjct: 428 ALISALTI 435 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 171 bits (434), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 127/400 (31%), Positives = 198/400 (49%), Gaps = 5/400 (1%) Query: 2 KTQEVSQIIDGARFTQFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGAL 61 +T ++ +++D F+ F +V+CL AL +V DG+D + G +P L+KEW ++ Sbjct: 10 QTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPA 69 Query: 62 GSYALFGMMFGAFVFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPGEFGIFRFLAGL 121 + L GM G+ G ADR GR+ + S +F V+T L F+ I RF AGL Sbjct: 70 VAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAGL 129 Query: 122 GCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAA 181 G GG +P++ + E++P R R+ V LGG+L +LP++GW+ +F Sbjct: 130 GIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLIG 189 Query: 182 AVPLVLLPVILW-KLPESVGFLLRAGKQ-EQARAMLAQVAPQAALGADVQLVQSDAKGSG 239 + V V+LW +PES +L R + ++ R +L +++ + + A + Sbjct: 190 GLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADAREQAIETR 249 Query: 240 APMLDLFKQERTLGTLMIWVAFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFG 299 A LF + T+ +WVAFF LL VY+ SWLP ++A G S + + L A NFG Sbjct: 250 AGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTAYNFG 309 Query: 300 GVVGAIGGGWLGDRF--TLPKVVVSFFAVGT-VAIALLGFNSPTPVLYLLIIVAGATTIG 356 GV GA+ RF LP ++ A + + + L S T +L + I + G Sbjct: 310 GVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGIGLHGLFVNA 369 Query: 357 TQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLG 396 Q +YA A YP +R+TG A GR GAI+ G Sbjct: 370 VQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAG 409 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 112/425 (26%), Positives = 194/425 (45%), Gaps = 4/425 (0%) Query: 5 EVSQIIDGARFTQFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGALGSY 64 V Q + A +F + + + L+ + D + G P + K + L+ QA G+ Sbjct: 7 SVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTM 66 Query: 65 ALFGMMFGAFVFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPGEFGIFRFLAGLGCG 124 GM+ GAFVFG LADRIGR+ + + V+ + S FA R L G+G G Sbjct: 67 FFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVG 126 Query: 125 GLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLP--NFGWQSMFFAAA 182 G +P +M E+ P R + ++ S +++G + A L + + N W+ +FF Sbjct: 127 GTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRVIFFVTG 186 Query: 183 VPLVLLPVILWKLPESVGFLLRAGKQEQARAMLAQVAPQAALGADVQLVQSDAKGSGAPM 242 +P ++ V+ + +PES +L R+GK + AR +L +VA + +Q + PM Sbjct: 187 LPALVGVVLRFYVPESPMYLNRSGKSDAARQVLERVAKVNGSSTPIPPLQPEVV-ERKPM 245 Query: 243 LDLFKQERTLGTLMIWVAFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGGVV 302 LF +L +++A+ + Y + +LP + S G+ FL+ L + Sbjct: 246 SALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLALVQLP 305 Query: 303 GAIGGGWLGDRFTLPKVVVSFFAVGTVAIALLGFNSPTPVLYLLIIVAGATTIGTQILLY 362 G + +R+ +V F + V L ++ ++ + +GT LY Sbjct: 306 GYALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGKDPMLVVGSTLLLSFSLLGTWAALY 365 Query: 363 ANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFL-VFAVPGLVAA 421 A T + YP +R++G+G A V R G + P + +MA+ L + L F V G VA Sbjct: 366 AFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAFLVAGAVAI 425 Query: 422 LAIGI 426 L + + Sbjct: 426 LCVDV 430 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 119 bits (298), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 114/431 (26%), Positives = 195/431 (45%), Gaps = 20/431 (4%) Query: 10 IDGARFTQFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGALGSYALFGM 69 I+ F+++H + +FD + VLPVL+ W LS Q G L + G Sbjct: 33 IENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQ 92 Query: 70 MFGAFVFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPGEFGIFRFLAGLGCGGLMPN 129 + GA FG +A+R GR +++ L SV + + S + RFL G+G GG +P Sbjct: 93 VIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPV 152 Query: 130 AVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAAAVPLVLLP 189 A +NE + R + + LG + A +G +++P FGW+ MF +P +L+ Sbjct: 153 AATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLVGGIPGILVA 212 Query: 190 VILWKLPESVGFLLRAGKQEQARAM---LAQVAPQAALGADV------QLVQSDAKGSGA 240 +++ +LPES +L+ G+ + A + L P+ L A Q + + A G+G Sbjct: 213 LLITRLPESPRWLIARGRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGK 272 Query: 241 PML----DLFKQERTLGTLMIWVAFFCCLLMVYALGSWLPKLMASAGY-SLGSSLSFLLA 295 +LF T+++W+ + + + +WLP L + + L SL Sbjct: 273 RQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASV 332 Query: 296 LNFGGVVGAIGGGWLGDRFTLPKVVVSFFAVGTVAIALLGFNSPTPVLYLLIIVAGATTI 355 N +G L DR + ++ F + +ALLG ++ + + A + Sbjct: 333 SNVLSACAVLGCALLIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLASSAYAV 392 Query: 356 --GTQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVF 413 T +LLY T + YP +R+ G G A+ R+ + P + G ++A + FL+F Sbjct: 393 MGTTTVLLYLYTPEIYPTRMRAIGTGLATSWLRAASAAAPAMVGVVLAGH-GIATVFLMF 451 Query: 414 A---VPGLVAA 421 A V GL+AA Sbjct: 452 AGVTVLGLLAA 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,216,975 Number of extensions: 46694 Number of successful extensions: 425 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 402 Number of HSP's successfully gapped: 31 Length of query: 455 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 367 Effective length of database: 1,203,190 Effective search space: 441570730 Effective search space used: 441570730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)