BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0538.p (341 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 601 e-173 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 428 e-121 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 108 5e-25 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 105 7e-24 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 88 8e-19 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 601 bits (1550), Expect = e-173, Method: Compositional matrix adjust. Identities = 287/341 (84%), Positives = 313/341 (91%) Query: 1 MAKQTILVTGGAGYIGSHTCVELLHAGYDVVVLDNLCNSRRSVIDRVAQISGRTPVFIEG 60 M + TILVTGGAGYIGSHTCVEL+HAGY VVVLDNLCNSR SVIDR+AQISG+ P F++G Sbjct: 1 MTQATILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQG 60 Query: 61 DIRDAALLDQIFGTHAIDAVIHFAGLKAVGESVQQPVRYYDNNVNGSNLLFAAMARHGVK 120 DIRD A+LD IF +H I+AVIHFAGLKAVGESV QP+RYYDNNV+GSN+LF AMA HGVK Sbjct: 61 DIRDRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVK 120 Query: 121 KLVFSSSATVYGDPASVPITEDFPLSATNPYGRSKLMVEQILGDIHVSDPDWHIALLRYF 180 +VFSSSATVYGDPASVPITE+FPLSATNPYGRSKLMVEQILGD+HV+DP W IALLRYF Sbjct: 121 NIVFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYF 180 Query: 181 NPVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRASLAVYGSDYPTVDGTGVRDYIHVVD 240 NPVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRA+L+VYGSDYPT DGTGVRDYIHVVD Sbjct: 181 NPVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVD 240 Query: 241 LALGHLKTLVKLGEGPGVRIYNLGTGRGNSVLEMLAAFEKACGKKLPHQLTARRPGDIAM 300 LALGHLKTL KL +GPGV YNLGTGRGNSVLEMLAAFE+ACG KLP+QL RRPGDIA Sbjct: 241 LALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIAC 300 Query: 301 CYAATDRAERELGWRAVRDVDAMCADTWRWQSLAKKLATEE 341 CYAAT+RAERELGWRA R ++AMCADTWRWQ+LAK+LA EE Sbjct: 301 CYAATERAERELGWRAQRGIEAMCADTWRWQALAKRLAQEE 341 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 428 bits (1100), Expect = e-121, Method: Compositional matrix adjust. Identities = 205/329 (62%), Positives = 249/329 (75%), Gaps = 1/329 (0%) Query: 5 TILVTGGAGYIGSHTCVELLHAGYDVVVLDNLCNSRRSVIDRVAQISGRTPVFIEGDIRD 64 ILVTGGAGYIGSHTCVELL AG++V+V DN CNS + RV QI+G+T ++GDIR+ Sbjct: 2 NILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIRN 61 Query: 65 AALLDQIFGTHAIDAVIHFAGLKAVGESVQQPVRYYDNNVNGSNLLFAAMARHGVKKLVF 124 L+ + +AVIHFAGLKAVGESV+QP+ YYDNNV G+ L AM GVK +VF Sbjct: 62 REQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIVF 121 Query: 125 SSSATVYGDPASVPITEDFPLSATNPYGRSKLMVEQILGDIHVSDPDWHIALLRYFNPVG 184 SSSATVYGDP +P+ E PLSA+NPYGRSK+++E +L D + + PDW IALLRYFNPVG Sbjct: 122 SSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPVG 181 Query: 185 AHESGLIGEDPSGIPNNLLPFIAQVADGRRASLAVYGSDYPTVDGTGVRDYIHVVDLALG 244 AHESGLIGEDP GIPNNL+PF+AQVA GRR L ++G DYPT DGTGVRD+IHVVDLA G Sbjct: 182 AHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLARG 241 Query: 245 HLKTLVKLGEGPGVRIYNLGTGRGNSVLEMLAAFEKACGKKLPHQLTARRPGDIAMCYAA 304 H+ L L + P NLGTG G SVL++L A+EKACGK LP+ + RRPGDIA CYA Sbjct: 242 HVAALQAL-DAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN 300 Query: 305 TDRAERELGWRAVRDVDAMCADTWRWQSL 333 A+R+LGW A ++AMCAD+W+WQ + Sbjct: 301 PAEAKRKLGWEATLGLEAMCADSWKWQQM 329 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 108 bits (271), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 91/348 (26%), Positives = 149/348 (42%), Gaps = 35/348 (10%) Query: 1 MAKQTILVTGGAGYIGSHTCVELLHAGYDVVVLDNLCNSRRSVIDRVAQISGRTPV---- 56 + +T LVTG AG+IGS+ LL VV LDN + +D V + TP Sbjct: 14 LTPRTWLVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALV--TPEQWAN 71 Query: 57 --FIEGDIRD-----AALLDQIFGTHAIDAVIHFAGLKAVGESVQQPVRYYDNNVNGSNL 109 F+EGDIR AA+ + G++ ++ V+H A L +V S++ P+ NN++G Sbjct: 72 FRFVEGDIRKLDDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLN 131 Query: 110 LFAAMARHGVKKLVFSSSATVYGDPASVPITEDFPLSATNPYGRSKLMVEQILGDIHVSD 169 + A VK+ V+++S++ YGD +P ED +PY +K V ++ D+ Sbjct: 132 MLVAARDARVKRFVYAASSSTYGDHPGLPKVEDRIGKPLSPYAVTKF-VNELYADVFART 190 Query: 170 PDWHIALLRYFNPVGAHESGLIGEDPSGIPNNLLP-FIAQVADGRRASLAVYGSDYPTVD 228 LRYFN G ++P G ++P +IA G + Y D Sbjct: 191 YGLQTIGLRYFNIFGRR------QNPDGAYAAVIPKWIAAAIKGEQI--------YINGD 236 Query: 229 GTGVRDYIHVVDLALGHLKTLVKLGEGPGVRIYNLGTGRGNSVLEMLAAFEKACGKKLPH 288 G RD+ +V + +L L + ++YN+ G ++ E+ PH Sbjct: 237 GETSRDFCYVDNAVQANLLAACTLQDEAANQVYNVAVGDRTTLKELYQFLRDNLSATFPH 296 Query: 289 QLTAR------RPGDIAMCYAATDRAERELGWRAVRDVDAMCADTWRW 330 A+ R GD+ A + R LG+ + ++ W Sbjct: 297 LRDAQPIFREFRAGDVRHSLADIGKGRRLLGYEPSHRIGRGLKESMNW 344 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 105 bits (261), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 94/334 (28%), Positives = 144/334 (43%), Gaps = 27/334 (8%) Query: 7 LVTGGAGYIGSHTCVELLHAGYDVVVLDNLCNSRRSVIDRVAQISGRTP----VFIEGDI 62 L+TG AG+IGS+ LL G V LDN R++ ++ V G FIEGDI Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAWANFRFIEGDI 78 Query: 63 RDAALLDQIFGTHAIDAVIHFAGLKAVGESVQQPVRYYDNNVNGSNLLFAAMARHGVKKL 122 DAA + D V+H A L +V S++ PVR NNVNG + A GV + Sbjct: 79 CDAATC--LRACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVARF 136 Query: 123 VFSSSATVYGDPASVPITEDFPLSATNPYGRSKLMVEQILGDIHVSDPDWHIALLRYFNP 182 V+++S++ YGD +P E+ +PY +K V ++ ++ LRYFN Sbjct: 137 VYAASSSTYGDHPGLPKVEEVIGKPLSPYAVTK-YVNELYAEVFARCYGMESIGLRYFNV 195 Query: 183 VGAHESGLIGEDPSGIPNNLLPFIAQVADGRRASLAVYGSDYPTVDGTGVRDYIHVVDLA 242 G +DP G ++P A R L + G DG RD+ ++ ++ Sbjct: 196 FGRR------QDPHGAYAAVIPQWV-TAMIRNGELRING------DGQTSRDFCYIDNVV 242 Query: 243 LGHLKTLVKLGEGPGVRIYNLGTGRGNSVLEMLAAFEKACGKKLPHQLTAR------RPG 296 +L GE ++YN+ S+ ++ A + ++ PH R R G Sbjct: 243 QANLLAATA-GEEAVNQVYNVALNDRTSLNDLYAMMRELLVQRFPHLQEHRPSYVGFRAG 301 Query: 297 DIAMCYAATDRAERELGWRAVRDVDAMCADTWRW 330 DI A +A + LG+ + A W Sbjct: 302 DIRDSQADIGKARQRLGYAPTHRLRAGLEQAMSW 335 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 88.2 bits (217), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 89/334 (26%), Positives = 144/334 (43%), Gaps = 28/334 (8%) Query: 4 QTILVTGGAGYIGSHTCVELLHAGY-DVVVLDNLCNSRRSVIDRVAQISGRTPVF-IEGD 61 + I+V GGAG IGSHT L+ ++++ DN R + + R ++ + GD Sbjct: 6 KKIVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNALK-DPRVKIYDVGGD 64 Query: 62 IRDAALLDQIFGTHAIDAVIHFAGLKAVGESVQQPVRYYDNNVNGSNLLFAAMARHGVKK 121 I +L D V H A L + + + P +D NV G+ + A GVK+ Sbjct: 65 IMQTDILQSAL--EGADGVFHLAALWLL-QCHEYPRSAFDVNVRGTFNVMEACVAKGVKR 121 Query: 122 LVFSSSATVYGDPASVPITEDFPLSATNPYGRSKLMVEQILGDIHVSDPDWHIALLRYFN 181 LV+SSSA+VYGD P+ ED P + N YG +K+ E +L H ++ LRY N Sbjct: 122 LVYSSSASVYGDAVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVG-LRYMN 180 Query: 182 PVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRASLAVYGSDYPTV--DGTGVRDYIHVV 239 G +D G + I ++ D A+ + PT+ DG+ D++ V Sbjct: 181 VYGPR------QDYHGA---YIAVIMKMLD------AIDKGESPTIMGDGSEAFDFVAVE 225 Query: 240 DLALGHLKTLVKLGEGPGVRIYNLGTGRGNSVLEMLAAFEKACGKKLPHQLTARRPGDIA 299 D L + + + + YN+GTG+ S+ E+ + G P R + Sbjct: 226 DCGLAN---VCAMKAETADQYYNVGTGKRTSLKELAELLLEITGCDKPITFAPRSQATLV 282 Query: 300 MC-YAATDRAERELGWRAVRDVDAMCADTWRWQS 332 +A E+G+ A D+ W++ Sbjct: 283 RNRIGCPKKASSEIGFTATIDLREGLKRLIEWRA 316 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,160,055 Number of extensions: 52376 Number of successful extensions: 213 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 208 Number of HSP's successfully gapped: 6 Length of query: 341 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 255 Effective length of database: 1,212,660 Effective search space: 309228300 Effective search space used: 309228300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)