BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0466.p (266 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 250 1e-67 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 100 9e-23 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 92 6e-20 gi|300312077|ref|YP_003776169.1| response regulator containing a... 58 8e-10 gi|300309415|ref|YP_003773507.1| two component response regulato... 57 1e-09 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 250 bits (638), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 127/128 (99%), Positives = 128/128 (100%) Query: 5 VDDSALIRSVMREIIGNQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFL 64 VDDSALIRSVMREIIG+QPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFL Sbjct: 8 VDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFL 67 Query: 65 EKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADKIR 124 EKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADKIR Sbjct: 68 EKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADKIR 127 Query: 125 AAAKARVR 132 AAAKARVR Sbjct: 128 AAAKARVR 135 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 100 bits (250), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 57/135 (42%), Positives = 81/135 (60%), Gaps = 1/135 (0%) Query: 5 VDDSALIRSVMREIIGNQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFL 64 +DDSAL+R + ++ ++ + + A EL + PDV+TLD+ MP MDGL L Sbjct: 7 IDDSALMRRQLMQVFEQAGGFDIC-LGRNGREAVELNRSFAPDVVTLDINMPEMDGLSAL 65 Query: 65 EKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADKIR 124 +M RP+PVVMVSSLTE+G+ T A+ LGA+D+V KP +I + E + K+R Sbjct: 66 SLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQDLLAKVR 125 Query: 125 AAAKARVRWGIPDPH 139 AAKAR+R P P Sbjct: 126 VAAKARIRSRAPAPE 140 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 91.7 bits (226), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 45/119 (37%), Positives = 77/119 (64%) Query: 12 RSVMREIIGNQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFLEKLMRLR 71 R +M++++ PD+ V+G APDP+ A ++Q PDV+ LD++MPRMDG+ FL ++M R Sbjct: 19 RQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMDGVSFLRQIMATR 78 Query: 72 PMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADKIRAAAKAR 130 P PV+M S+ + + AL GA+ + +P++ ++ +Q+ + +A IRAA +AR Sbjct: 79 PTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEELASAIRAAMQAR 137 >gi|300312077|ref|YP_003776169.1| response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 217 Score = 57.8 bits (138), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 34/99 (34%), Positives = 61/99 (61%), Gaps = 3/99 (3%) Query: 6 DDSALIRSVMREIIGNQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFLE 65 DD A++R +R+I D+ V G A + + A +L ++ N DVL LD+ +P +G+D L+ Sbjct: 15 DDHAIVREGLRQICAGSKDIMVAGNAANGLEAIKLAREGNGDVLLLDIVLPDRNGIDILK 74 Query: 66 KLMRLRP-MPVVMVSSLTERGSEITLRALELGAIDFVTK 103 ++ + +P +PV+++S R + +RAL+ GA F+ K Sbjct: 75 QIRKEQPELPVLILS--IHREDQYAIRALKAGAAGFLNK 111 >gi|300309415|ref|YP_003773507.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 39/111 (35%), Positives = 64/111 (57%), Gaps = 7/111 (6%) Query: 5 VDDSALIRSVMREIIGNQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFL 64 VDD IR ++ EI+G++ VV A + AREL + PD++ LD+ MP DG+ L Sbjct: 7 VDDEMGIRELLSEILGDEG--HVVTTAENAQQARELRQAGVPDLVLLDIWMPDTDGVTLL 64 Query: 65 EKLMR--LRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQ 113 ++ R L MPV+M+S + + A +GA++F+ KP I++Q ++ Sbjct: 65 KEWQRDGLLTMPVIMMSG--HATIDTAVEATRIGALNFLEKP-IALQKLLK 112 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.140 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 971,260 Number of extensions: 45584 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 231 Number of HSP's successfully gapped: 9 Length of query: 266 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 182 Effective length of database: 1,222,130 Effective search space: 222427660 Effective search space used: 222427660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)